EPR Plasmid #1075: pTRIP-mNFM-EGFP-StrepII
Plasmid Details
| Purpose | mammalian expression and/or lentiviral production of mouse NFM fused to EGFP and a TwinStrep purification tag. The coding sequence of NFM is cloned from mus musculus brain cDNA corresponding to NP_032717 |
| Depositing Lab |
Carsten Janke Institut Curie |
| Depositing Scientist | Veronique Henriot |
| Antibiotic Resistance | Ampicillin |
| Bacterial Growth Temp | 37 |
| Copy Number | High |
| Selectable Marker | |
| Final expression system: | Mammalian cells |
| Vector type: | Lentiviral |
| Insert type: | cDNA / ORF |
| Insert species: | Mouse |
| Insert gene: | NFM |
| Reference |
SILAC-based discovery of a broad range of proteins that can be posttranslationally glutamylated or glycylated. Dey S et al. J Cell Sci 2026 PubMed DOI See other plasmids from this publication |
| MTA and Terms |
MTA (PDF download) |
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download annotated sequence file provided by depositor
Design a cloning with
BioNexio
How to Cite This Reagent
IN THE MATERIALS & METHODS:
pTRIP-mNFM-EGFP-StrepII was a gift from Carsten Janke (European Plasmid Repository plasmid #1075).
pTRIP-mNFM-EGFP-StrepII was a gift from Carsten Janke (European Plasmid Repository plasmid #1075).
IN THE REFERENCES:
Dey S et al.. SILAC-based discovery of a broad range of proteins that can be posttranslationally glutamylated or glycylated.. J Cell Sci 2026 doi:10.1242/jcs.264734 PubMed 42108952
Dey S et al.. SILAC-based discovery of a broad range of proteins that can be posttranslationally glutamylated or glycylated.. J Cell Sci 2026 doi:10.1242/jcs.264734 PubMed 42108952
Sequences
Provided by depositor
download sequence
acataattggacaaactacctacagagatttaaagctctaaggtaaatataaaatttttaagtgtataatgtgttaaactactgattctaattgtttgtgtattttagattccaacctatggaactgatgaatgggagcagtggtggaatgcctttaatgaggaaaacctgttttgctcagaagaaatgccatctagtgatgatgaggctactgctgactctcaacattctactcctccaaaaaagaagagaaaggtagaagaccccaaggactttccttcagaattgctaagttttttgagtcatgctgtgtttagtaatagaactcttgcttgctttgctatttacaccacaaaggaaaaagctgcactgctatacaagaaaattatggaaaaatattctgtaacctttataagtaggcataacagttataatcataacatactgttttttcttactccacacaggcatagagtgtctgctattaataactatgctcaaaaattgtgtacctttagctttttaatttgtaaaggggttaataaggaatatttgatgtatagtgccttgactagagatcataatcagccataccacatttgtagaggttttacttgctttaaaaaacctcccacacctccccctgaacctgaaacataaaatgaatgcaattgttgttgttaacttgtttattgcagcttataatggttacaaataaagcaatagcatcacaaatttcacaaataaagcatttttttcactgcattctagttgtggtttgtccaaactcatcaatgtatcttatcatgtctggatcaactggataactcaagctaaccaaaatcatcccaaacttcccaccccataccctattaccactgccaattacctgtggtttcatttactctaaacctgtgattcctctgaattattttcattttaaagaaattgtatttgttaaatatgtactacaaacttagtagttggaagggctaattcactcccaaagaagacaagatatccttgatctgtggatctaccacacacaaggctacttccctgattagcagaactacacaccagggccaggggtcagatatccactgacctttggatggtgctacaagctagtaccagttgagccagataaggtagaagaggccaataaaggagagaacaccagcttgttacaccctgtgagcctgcatgggatggatgacccggagagagaagtgttagagtggaggtttgacagccgcctagcatttcatcacgtggcccgagagctgcatccggagtacttcaagaactgctgatatcgagcttgctacaagggactttccgctggggactttccagggaggcgtggcctgggcgggactggggagtggcgagccctcagatcctgcatataagcagctgctttttgcctgtactgggtctctctggttagaccagatctgagcctgggagctctctggctaactagggaacccactgcttaagcctcaataaagcttgccttgagtgcttcaagtagtgtgtgcccgtctgttgtgtgactctggtaactagagatccctcagacccttttagtcagtgtggaaaatctctagcagtggcgcccgaacagggacttgaaagcgaaagggaaaccagaggagctctctcgacgcaggactcggcttgctgaagcgcgcacggcaagaggcgaggggcggcgactggtgagtacgccaaaaattttgactagcggaggctagaaggagagagatgggtgcgagagcgtcagtattaagcgggggagaattagatcgcgatgggaaaaaattcggttaaggccagggggaaagaaaaaatataaattaaaacatatagtatgggcaagcagggagctagaacgattcgcagttaatcctggcctgttagaaacatcagaaggctgtagacaaatactgggacagctacaaccatcccttcagacaggatcagaagaacttagatcattatataatacagtagcaaccctctattgtgtgcatcaaaggatagagataaaagacaccaaggaagctttagacaagatagaggaagagcaaaacaaaagtaagaccaccgcacagcaagcggccgctgatcttcagacctggaggaggagatatgagggacaattggagaagtgaattatataaatataaagtagtaaaaattgaaccattaggagtagcacccaccaaggcaaagagaagagtggtgcagagagaaaaaagagcagtgggaataggagctttgttccttgggttcttgggagcagcaggaagcactatgggcgcagcgtcaatgacgctgacggtacaggccagacaattattgtctggtatagtgcagcagcagaacaatttgctgagggctattgaggcgcaacagcatctgttgcaactcacagtctggggcatcaagcagctccaggcaagaatcctggctgtggaaagatacctaaaggatcaacagctcctggggatttggggttgctctggaaaactcatttgcaccactgctgtgccttggaatgctagttggagtaataaatctctggaacagatttggaatcacacgacctggatggagtgggacagagaaattaacaattacacaagcttaatacactccttaattgaagaatcgcaaaaccagcaagaaaagaatgaacaagaattattggaattagataaatgggcaagtttgtggaattggtttaacataacaaattggctgtggtatataaaattattcataatgatagtaggaggcttggtaggtttaagaatagtttttgctgtactttctatagtgaatagagttaggcagggatattcaccattatcgtttcagacccacctcccaaccccgaggggacccgacaggcccgaaggaatagaagaagaaggtggagagagagacagagacagatccattcgattagtgaacggatctcgacggtatcgccgaattcacaaatggcagtattcatccacaattttaaaagaaaaggggggattggggggtacagtgcaggggaaagaatagtagacataatagcaacagacatacaaactaaagaattacaaaaacaaattacaaaaattcaaaattttcgggtttattacagggacagcagagatccactttggctgatacgcgtTGACATTGATTATTGACTAGTTATTAATAGTAATCAATTACGGGGTCATTAGTTCATAGCCCATATATGGAGTTCCGCGTTACATAACTTACGGTAAATGGCCCGCCTGGCTGACCGCCCAACGACCCCCGCCCATTGACGTCAATAATGACGTATGTTCCCATAGTAACGCCAATAGGGACTTTCCATTGACGTCAATGGGTGGACTATTTACGGTAAACTGCCCACTTGGCAGTACATCAAGTGTATcatatgccaagtacgccccctattgacgtcaatgacggtaaatggcccgcctggcattatgcccagtacatgaccttatgggactttcctacttggcagtacatctacgtattagtcatcgctattaccatgggtcgaggtgagccccacgttctgcttcactctccccatctcccccccctccccacccccaattttgtatttatttattttttaattattttgtgcagcgatgggggcggggggggggggggcgcgcgccaggcggggcggggcggggcgaggggcggggcggggcgaggcggagaggtgcggcggcagccaatcagagcggcgcgctccgaaagtttccttttatggcgaggcggcggcggcggcggccctataaaaagcgaagcgcgcggcgggcgggagtcgctgcgttgccttcgccccgtgccccgctccgcgccgcctcgcgccgcccgccccggctctgactgaccgcgttactcccacaggtgagcgggcgggacggcccttctcctccgggctgtaattagcgcttggtttaatgacggctcgtttcttttctgtggctgcgtgaaagccttaaagggctccgggagggccctttgtgcgggggggagcggctcggggggtgcgtgcgtgtgtgtgtgcgtggggagcgccgcgtgcggcccgcgctgcccggcggctgtgagcgctgcgggcgcggcgcggggctttgtgcgctccgcgtgtgcgcgaggggagcgcggccgggggcggtgccccgcggtgcgggggggctgcgaggggaacaaaggctgcgtgcggggtgtgtgcgtgggggggtgagcagggggtgtgggcgcggcggtcgggctgtaacccccccctgcacccccctccccgagttgctgagcacggcccggcttcgggtgcggggctccgtgcggggcgtggcgcggggctcgccgtgccgggcggggggtggcggcaggtgggggtgccgggcggggcggggccgcctcgggccggggagggctcgggggaggggcgcggcggccccggagcgccggcggctgtcgaggcgcggcgagccgcagccattgccttttatggtaatcgtgcgagagggcgcagggacttcctttgtcccaaatctggcggagccgaaatctgggaggcgccgccgcaccccctctagcgggcgcgggcgaagcggtgcggcgccggcaggaaggaaatgggcggggagggccttcgtgcgtcgccgcgccgccgtccccttctccatctccagcctcggggctgccgcagggggacggctgccttcgggggggacggggcagggcggggttcggcttctggcgtgtgaccggcggctctagagcctctgctaaccatgttcatgccttcttctttttcctacagctcctgggcaacgtgctggttattgtgctgtctcatcattttggcaaagaattattccgctagCATGAGCTACACGCTGGACTCGCTGGGCAACCCGTCCGCCTACCGGCGGGTCACCGAGACCCGCTCCAGCTTCAGCCGCGTGAGCGGTTCCCCGTCCAGCGGCTTCCGCTCGCAGTCCTGGTCCCGCGGCTCGCCCAGCACCGTGTCCTCCTCCTACAAGCGCAGCGCGCTCGCCCCGCGTCTCGCCTACAGCTCGGCTATGCTCAGCTCGGCCGAGAGCAGCCTCGACTTCAGCCAGTCCTCGTCGCTGCTCAACGGCGGCTCCGGCGGCGACTACAAACTGTCCCGCTCTAACGAGAAAGAGCAGCTGCAGGGGCTGAACGACCGCTTCGCCGGCTACATCGAGAAAGTGCACTACTTGGAACAACAGAACAAGGAGATCGAAGCAGAGATCCAGGCACTGCGGCAGAAGCAGGCCTCGCACGCCCAGCTGGGTGATGCTTACGACCAGGAGATCCGAGAGCTGCGCGCCACCCTCGAGATGGTGAACCACGAGAAGGCTCAAGTGCAGCTGGACTCCGATCACTTGGAGGAAGACATCCACCGGCTCAAGGAGCGCTTCGAGGAGGAGGCGCGGCTGCGGGACGACACCGAGGCTGCCATTCGCGCGCTGCGCAAAGACATCGAAGAGTCGTCGATGGTTAAGGTGGAGCTGGACAAGAAGGTGCAGTCGCTGCAGGATGAGGTGGCTTTCCTGCGGAGCAATCACGAAGAGGAGGTGGCCGACCTGCTGGCTCAGATCCAGGCGTCGCACATCACGGTAGAGCGCAAAGATTACCTGAAGACAGACATCTCCACGGCGCTGAAGGAGATCCGCTCCCAGCTCGAGTGTCACTCAGACCAGAACATGCACCAGGCCGAAGAGTGGTTCAAATGCCGCTACGCCAAGCTCACCGAGGCGGCCGAGCAGAACAAGGAGGCCATTCGCTCTGCCAAGGAAGAGATCGCCGAGTACCGGCGCCAGCTGCAGTCCAAGAGCATCGAGCTCGAGTCGGTGCGAGGCACTAAGGAGTCCCTGGAGCGGCAGCTCAGCGACATCGAGGAGCGCCACAACCACGACCTCAGCAGCTACCAGGACACCATCCAGCAGTTGGAAAATGAACTTCGGGGAACCAAGTGGGAAATGGCTCGTCATTTGCGAGAATACCAGGATCTCCTTAACGTCAAGATGGCCCTGGACATCGAGATCGCCGCGTACAGGAAACTCCTAGAGGGGGAAGAGACCAGATTTAGCACATTTTCAGGAAGCATCACCGGGCCTCTGTACACACACCGACAGCCCTCAGTCACAATATCCAGTAAGATTCAGAAGACCAAAGTCGAGGCCCCCAAGCTCAAGGTCCAACACAAATTTGTGGAGGAGATCATCGAAGAAACTAAAGTGGAAGATGAGAAGTCAGAAATGGAAGAAACCCTCACAGCCATCGCAGAGGAGTTGGCAGCCTCCGCCAAAGAGGAGAAGGAAGAGGCCGAAGAAAAGGAGGAGGAACCAGAAGCCGAAAAGTCTCCCGTGAAGTCTCCTGAGGCTAAGGAAGAGGAGGAGGAAGGGGAAAAGGAGGAAGAAGAGGAAGGCCAGGAGGAAGAAGAGGAGGAAGATGAAGGTGTCAAGTCAGACCAGGCAGAAGAGGGGGGATCTGAGAAGGAAGGCTCCAGTGAAAAAGATGAAGGTGAGCAGGAAGAAGAAGAAGGAGAAACCGAGGCAGAAGGTGAAGGAGAGGAAGCAGAAGCTAAGGAGGAAAAGAAAATTGAGGGAAAGGTTGAGGAAGTGGCTGTCAAGGAGGAAATCAAGGTCGAGAAGCCTGAGAAAGCCAAATCCCCTATGCCCAAATCACCCGTGGAAGAAGTAAAGCCAAAACCAGAGGCCAAGGCCGGGAAGGGTGAGCACAAGGAGGAAGAGAAAGTTGAGGAAGAGAAGAAGGAAGTCACCAAAGAATCACCCAAGGAAGAGAAGGTGGAGAAAAAGGAGGAGAAGCCAAAAGATGTTGCAGATAAAAAGAAGGCCGAGTCCCCGGTGAAAGAGAAGGCTGTGGAGGAGGTGATCACCATCAGCAAGTCGGTAAAGGTGAGCCTAGAGAAAGACACCAAAGAGGAGAAGCTGCAGCCGCAGGAGAAGGTGAAGGAGAAGGCAGAGGAGGAGGGGGGCAGTGAGGAGGAAGGGAGTGACCGTAGCCCGCAGGAGTCCAAGAAGGAAGACATAGCTATCAATGGGGAGGTGGAAGGAAAAGAGGAGGAGGAGCAGGAAACTCAGGAGAAGGGCAGTGGGCGGGAGGAGGAGAAAGGGGTGGTCACTAATGGCTTAGATGTGAGCCCTGCAGAGGAGAAGAAAGGAGAGGATAGCAGTGATGATAAAGTGGTGGTCACCAAGAAGGTAGAAAAGATCACCAGCGAGGGGGGCGATGGTGCTACCAAATACATCACCAAATCTGTAACCGTCACTCAAAAGGTTGAAGAGCATGAGGAGACCTTTGAGGAGAAGCTGGTCTCAACTAAAAAGGTAGAAAAGGTCACTTCACACGCCATAGTCAAGGAAGTCACCCAGGGTGACGctagcGCCACCatggtgagcaagggcgaggagctgttcaccggggtggtgcccatcctggtcgagctggacggcgacgtaaacggccacaagttcagcgtgtccggcgagggcgagggcgatgccacctacggcaagctgaccctgaagttcatctgcaccaccggcaagctgcccgtgccctggcccaccctcgtgaccaccctgacctacggcgtgcagtgcttcagccgctaccccgaccacatgaagcagcacgacttcttcaagtccgccatgcccgaaggctacgtccaggagcgcaccatcttcttcaaggacgacggcaactacaagacccgcgccgaggtgaagttcgagggcgacaccctggtgaaccgcatcgagctgaagggcatcgacttcaaggaggacggcaacatcctggggcacaagctggagtacaactacaacagccacaacgtctatatcatggccgacaagcagaagaacggcatcaaggtgaacttcaagatccgccacaacatcgaggacggcagcgtgcagctcgccgaccactaccagcagaacacccccatcggcgacggccccgtgctgctgcccgacaaccactacctgagcacccagtccgccctgagcaaagaccccaacgagaagcgcgatcacatggtcctgctggagttcgtgaccgccgccgggatcactctcggcatggacgagcTGTACAAGACTAGTGGCAGCGCTTGGAGtCAtCCaCAaTTtGAgAAgggcggcggcagtggcggcggcagtggcggcagtgccTGGAGCCACCCGCAGTTCGAAAAATAAggatccactagtgtcgacctcgagacctagaaaaacatggagcaatcacaagtagcaatacagcagctaccaatgctgattgtgcctggctagaagcacaagaggaggaggaggtgggttttccagtcacacctcaggtacctttaagaccaatgacttacaaggcagctgtagatcttagccactttttaaaagaaaaggggggactggaagggctaattcactcccaacgaagacaaaatcgtcgagagatgctgcatataagcagctgctttttgcttgtactgggtctctctggttagaccagatctgagcctgggagctctctggctaactagggaacccactgcttaagcctcaataaagcttgccttgagtgcttcaagtagtgtgtgcccgtctgttgtgtgactctggtaactagagatccctcagacccttttagtcagtgtggaaaatctctagcagtagtagttcatgtcatcttattattcagtatttataacttgcaaagaaatgaatatcagagagtgagaggccttgacattataatagatttagcaggaattgaactaggagtggagcacacaggcaaagctgcagaagtacttggaagaagccaccagagatactcacgattctgcacatacctggctaatcccagatcctaaggattacattaagtttactaacatttatataatgatttatagtttaaagtataaacttatctaatttactattctgacagatattaattaatcctcaaatatcataagagatgattactattatccccatttaacacaagaggaaactgagagggaaagatgttgaagtaattttcccacaattacagcatccgttagttacgactctatgatcttctgacacaaattccatttactcctcaccctatgactcagtcgaatatatcaaagttatggacattatgctaagtaacaaattacccttttatatagtaaatactgagtagattgagagaagaaattgtttgcaaacctgaatagcttcaagaagaagagaagtgaggataagaataacagttgtcatttaacaagttttaacaagtaacttggttagaaagggattcaaatgcataaagcaagggataaatttttctggcaacaagactatacaatataaccttaaatatgacttcaaataattgttggaacttgataaaactaattaaatattattgaagattatcaatattataaatgtaatttacttttaaaaagggaacatagaaatgtgtatcattagagtagaaaacaatccttattatcacaatttgtcaaaacaagtttgttattaacacaagtagaatactgcattcaattaagttgactgcagattttgtgttttgttaaaattagaaagagataacaacaatttgaattattgaaagtaacatgtaaatagttctacatacgttcttttgacatcttgttcaatcattgatcgaagttctttatcttggaagaatttgttccaaagactctgaaataaggaaaacaatctattatatagtctcacacctttgttttacttttagtgatttcaatttaataatgtaaatggttaaaatttattcttctctgagatcatttcacattgcagatagaaaacctgagactggggtaatttttattaaaatctaatttaatctcagaaacacatctttattctaacatcaatttttccagtttgatattatcatataaagtcagccttcctcatctgcaggttccacaacaaaaatccaaccaactgtggatcaaaaatattgggaaaaaattaaaaatagcaatacaacaataaaaaaatacaaatcagaaaaacagcacagtataacaactttatttagcatttacaatctattaggtattataagtaatctagccagatcctctacgccggacgcatcgtggccggcatcaccggcgccacaggtgcggttgctggcgcctatatcgccgacatcaccgatggggaagatcgggctcgccacttcgggctcatgagcgcttgtttcggcgtgggtatggtggcaggccccgtggccgggggactgttgggcgccatctccttgcatgcaccattccttgcggcggcggtgctcaacggcctcaacctactactgggctgcttcctaatgcaggagtcgcataagggagagcgtcgaatggtgcactctcagtacaatctgctctgatgccgcatagttaagccagccccgacacccgccaacacccgctgacgcgccctgacgggcttgtctgctcccggcatccgcttacagacaagctgtgaccgtctccgggagctgcatgtgtcagaggttttcaccgtcatcaccgaaacgcgcgagacgaaagggcctcgtgatacgcctatttttataggttaatgtcatgataataatggtttcttagacgtcaggtggcacttttcggggaaatgtgcgcggaacccctatttgtttatttttctaaatacattcaaatatgtatccgctcatgagacaataaccctgataaatgcttcaataatattgaaaaaggaagagtatgagtattcaacatttccgtgtcgcccttattcccttttttgcggcattttgccttcctgtttttgctcacccagaaacgctggtgaaagtaaaagatgctgaagatcagttgggtgcacgagtgggttacatcgaactggatctcaacagcggtaagatccttgagagttttcgccccgaagaacgttttccaatgatgagcacttttaaagttctgctatgtggcgcggtattatcccgtattgacgccgggcaagagcaactcggtcgccgcatacactattctcagaatgacttggttgagtactcaccagtcacagaaaagcatcttacggatggcatgacagtaagagaattatgcagtgctgccataaccatgagtgataacactgcggccaacttacttctgacaacgatcggaggaccgaaggagctaaccgcttttttgcacaacatgggggatcatgtaactcgccttgatcgttgggaaccggagctgaatgaagccataccaaacgacgagcgtgacaccacgatgcctgtagcaatggcaacaacgttgcgcaaactattaactggcgaactacttactctagcttcccggcaacaattaatagactggatggaggcggataaagttgcaggaccacttctgcgctcggcccttccggctggctggtttattgctgataaatctggagccggtgagcgtgggtctcgcggtatcattgcagcactggggccagatggtaagccctcccgtatcgtagttatctacacgacggggagtcaggcaactatggatgaacgaaatagacagatcgctgagataggtgcctcactgattaagcattggtaactgtcagaccaagtttactcatatatactttagattgatttaaaacttcatttttaatttaaaaggatctaggtgaagatcctttttgataatctcatgaccaaaatcccttaacgtgagttttcgttccactgagcgtcagaccccgtagaaaagatcaaaggatcttcttgagatcctttttttctgcgcgtaatctgctgcttgcaaacaaaaaaaccaccgctaccagcggtggtttgtttgccggatcaagagctaccaactctttttccgaaggtaactggcttcagcagagcgcagataccaaatactgttcttctagtgtagccgtagttaggccaccacttcaagaactctgtagcaccgcctacatacctcgctctgctaatcctgttaccagtggctgctgccagtggcgataagtcgtgtcttaccgggttggactcaagacgatagttaccggataaggcgcagcggtcgggctgaacggggggttcgtgcacacagcccagcttggagcgaacgacctacaccgaactgagatacctacagcgtgagctatgagaaagcgccacgcttcccgaagggagaaaggcggacaggtatccggtaagcggcagggtcggaacaggagagcgcacgagggagcttccagggggaaacgcctggtatctttatagtcctgtcgggtttcgccacctctgacttgagcgtcgatttttgtgatgctcgtcaggggggcggagcctatggaaaaacgccagcaacgcggcctttttacggttcctggccttttgctggccttttgctcacatgttctttcctgcgttatcccctgattctgtggataaccgtattaccgcctttgagtgagctgataccgctcgccgcagccgaacgaccgagcgcagcgagtcagtgagcgaggaagcggaagagcgcccaatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctgtggaatgtgtgtcagttagggtgtggaaagtccccaggctccccagcaggcagaagtatgcaaagcatgcatctcaattagtcagcaaccaggtgtggaaagtccccaggctccccagcaggcagaagtatgcaaagcatgcatctcaattagtcagcaaccatagtcccgcccctaactccgcccatcccgcccctaactccgcccagttccgcccattctccgccccatggctgactaattttttttatttatgcagaggccgaggccgcctcggcctctgagctattccagaagtagtgaggaggcttttttggaggcctaggcttttgcaaaaagcttggacacaagacaggcttgcgagatatgtttgagaataccactttatcccgcgtcagggagaggcagtgcgtaaaaagacgcggactcatgtgaaatactggtttttagtgcgccagatctctataatctcgcgcaacctattttcccctcgaacactttttaagccgtagataaacaggctgggacacttcacatgagcgaaaaatacatcgtcacctgggacatgttgcagatccatgcacgtaaactcgcaagccgactgatgccttctgaacaatggaaaggcattattgccgtaagccgtggcggtctgtaccgggtgcgttactggcgcgtgaactgggtattcgtcatgtcgataccgtttgtatttccagctacgatcacgacaaccagcgcgagcttaaagtgctgaaacgcgcagaaggcgatggcgaaggcttcatcgttattgatgacctggtggataccggtggtactgcggttgcgattcgtgaaatgtatccaaaagcgcactttgtcaccatcttcgcaaaaccggctggtcgtccgctggttgatgactatgttgttgatatcccgcaagatacctggattgaacagccgtgggatatgggcgtcgtattcgtcccgccaatctccggtcgctaatcttttcaacgcctggcactgccgggcgttgttctttttaacttcaggcgggttacaatagtttccagtaagtattctggaggctgcatccatgacacaggcaaacctgagcgaaaccctgttcaaaccccgctttaaacatcctgaaacctcgacgctagtccgccgctttaatcacggcgcacaaccgcctgtgcagtcggcccttgatggtaaaaccatccctcactggtatcgcatgattaaccgtctgatgtggatctggcgcggcattgacccacgcgaaatcctcgacgtccaggcacgtattgtgatgagcgatgccgaacgtaccgacgatgatttatacgatacggtgattggctaccgtggcggcaactggatttatgagtgggccccggatctttgtgaaggaaccttacttctgtggtgtgacataattggacaaactacctacagagatttaaagctctaaggtaatataaa
acataattggacaaactacctacagagatttaaagctctaaggtaaatataaaatttttaagtgtataatgtgttaaactactgattctaattgtttgtgtattttagattccaacctatggaactgatgaatgggagcagtggtggaatgcctttaatgaggaaaacctgttttgctcagaagaaatgccatctagtgatgatgaggctactgctgactctcaacattctactcctccaaaaaagaagagaaaggtagaagaccccaaggactttccttcagaattgctaagttttttgagtcatgctgtgtttagtaatagaactcttgcttgctttgctatttacaccacaaaggaaaaagctgcactgctatacaagaaaattatggaaaaatattctgtaacctttataagtaggcataacagttataatcataacatactgttttttcttactccacacaggcatagagtgtctgctattaataactatgctcaaaaattgtgtacctttagctttttaatttgtaaaggggttaataaggaatatttgatgtatagtgccttgactagagatcataatcagccataccacatttgtagaggttttacttgctttaaaaaacctcccacacctccccctgaacctgaaacataaaatgaatgcaattgttgttgttaacttgtttattgcagcttataatggttacaaataaagcaatagcatcacaaatttcacaaataaagcatttttttcactgcattctagttgtggtttgtccaaactcatcaatgtatcttatcatgtctggatcaactggataactcaagctaaccaaaatcatcccaaacttcccaccccataccctattaccactgccaattacctgtggtttcatttactctaaacctgtgattcctctgaattattttcattttaaagaaattgtatttgttaaatatgtactacaaacttagtagttggaagggctaattcactcccaaagaagacaagatatccttgatctgtggatctaccacacacaaggctacttccctgattagcagaactacacaccagggccaggggtcagatatccactgacctttggatggtgctacaagctagtaccagttgagccagataaggtagaagaggccaataaaggagagaacaccagcttgttacaccctgtgagcctgcatgggatggatgacccggagagagaagtgttagagtggaggtttgacagccgcctagcatttcatcacgtggcccgagagctgcatccggagtacttcaagaactgctgatatcgagcttgctacaagggactttccgctggggactttccagggaggcgtggcctgggcgggactggggagtggcgagccctcagatcctgcatataagcagctgctttttgcctgtactgggtctctctggttagaccagatctgagcctgggagctctctggctaactagggaacccactgcttaagcctcaataaagcttgccttgagtgcttcaagtagtgtgtgcccgtctgttgtgtgactctggtaactagagatccctcagacccttttagtcagtgtggaaaatctctagcagtggcgcccgaacagggacttgaaagcgaaagggaaaccagaggagctctctcgacgcaggactcggcttgctgaagcgcgcacggcaagaggcgaggggcggcgactggtgagtacgccaaaaattttgactagcggaggctagaaggagagagatgggtgcgagagcgtcagtattaagcgggggagaattagatcgcgatgggaaaaaattcggttaaggccagggggaaagaaaaaatataaattaaaacatatagtatgggcaagcagggagctagaacgattcgcagttaatcctggcctgttagaaacatcagaaggctgtagacaaatactgggacagctacaaccatcccttcagacaggatcagaagaacttagatcattatataatacagtagcaaccctctattgtgtgcatcaaaggatagagataaaagacaccaaggaagctttagacaagatagaggaagagcaaaacaaaagtaagaccaccgcacagcaagcggccgctgatcttcagacctggaggaggagatatgagggacaattggagaagtgaattatataaatataaagtagtaaaaattgaaccattaggagtagcacccaccaaggcaaagagaagagtggtgcagagagaaaaaagagcagtgggaataggagctttgttccttgggttcttgggagcagcaggaagcactatgggcgcagcgtcaatgacgctgacggtacaggccagacaattattgtctggtatagtgcagcagcagaacaatttgctgagggctattgaggcgcaacagcatctgttgcaactcacagtctggggcatcaagcagctccaggcaagaatcctggctgtggaaagatacctaaaggatcaacagctcctggggatttggggttgctctggaaaactcatttgcaccactgctgtgccttggaatgctagttggagtaataaatctctggaacagatttggaatcacacgacctggatggagtgggacagagaaattaacaattacacaagcttaatacactccttaattgaagaatcgcaaaaccagcaagaaaagaatgaacaagaattattggaattagataaatgggcaagtttgtggaattggtttaacataacaaattggctgtggtatataaaattattcataatgatagtaggaggcttggtaggtttaagaatagtttttgctgtactttctatagtgaatagagttaggcagggatattcaccattatcgtttcagacccacctcccaaccccgaggggacccgacaggcccgaaggaatagaagaagaaggtggagagagagacagagacagatccattcgattagtgaacggatctcgacggtatcgccgaattcacaaatggcagtattcatccacaattttaaaagaaaaggggggattggggggtacagtgcaggggaaagaatagtagacataatagcaacagacatacaaactaaagaattacaaaaacaaattacaaaaattcaaaattttcgggtttattacagggacagcagagatccactttggctgatacgcgtTGACATTGATTATTGACTAGTTATTAATAGTAATCAATTACGGGGTCATTAGTTCATAGCCCATATATGGAGTTCCGCGTTACATAACTTACGGTAAATGGCCCGCCTGGCTGACCGCCCAACGACCCCCGCCCATTGACGTCAATAATGACGTATGTTCCCATAGTAACGCCAATAGGGACTTTCCATTGACGTCAATGGGTGGACTATTTACGGTAAACTGCCCACTTGGCAGTACATCAAGTGTATcatatgccaagtacgccccctattgacgtcaatgacggtaaatggcccgcctggcattatgcccagtacatgaccttatgggactttcctacttggcagtacatctacgtattagtcatcgctattaccatgggtcgaggtgagccccacgttctgcttcactctccccatctcccccccctccccacccccaattttgtatttatttattttttaattattttgtgcagcgatgggggcggggggggggggggcgcgcgccaggcggggcggggcggggcgaggggcggggcggggcgaggcggagaggtgcggcggcagccaatcagagcggcgcgctccgaaagtttccttttatggcgaggcggcggcggcggcggccctataaaaagcgaagcgcgcggcgggcgggagtcgctgcgttgccttcgccccgtgccccgctccgcgccgcctcgcgccgcccgccccggctctgactgaccgcgttactcccacaggtgagcgggcgggacggcccttctcctccgggctgtaattagcgcttggtttaatgacggctcgtttcttttctgtggctgcgtgaaagccttaaagggctccgggagggccctttgtgcgggggggagcggctcggggggtgcgtgcgtgtgtgtgtgcgtggggagcgccgcgtgcggcccgcgctgcccggcggctgtgagcgctgcgggcgcggcgcggggctttgtgcgctccgcgtgtgcgcgaggggagcgcggccgggggcggtgccccgcggtgcgggggggctgcgaggggaacaaaggctgcgtgcggggtgtgtgcgtgggggggtgagcagggggtgtgggcgcggcggtcgggctgtaacccccccctgcacccccctccccgagttgctgagcacggcccggcttcgggtgcggggctccgtgcggggcgtggcgcggggctcgccgtgccgggcggggggtggcggcaggtgggggtgccgggcggggcggggccgcctcgggccggggagggctcgggggaggggcgcggcggccccggagcgccggcggctgtcgaggcgcggcgagccgcagccattgccttttatggtaatcgtgcgagagggcgcagggacttcctttgtcccaaatctggcggagccgaaatctgggaggcgccgccgcaccccctctagcgggcgcgggcgaagcggtgcggcgccggcaggaaggaaatgggcggggagggccttcgtgcgtcgccgcgccgccgtccccttctccatctccagcctcggggctgccgcagggggacggctgccttcgggggggacggggcagggcggggttcggcttctggcgtgtgaccggcggctctagagcctctgctaaccatgttcatgccttcttctttttcctacagctcctgggcaacgtgctggttattgtgctgtctcatcattttggcaaagaattattccgctagCATGAGCTACACGCTGGACTCGCTGGGCAACCCGTCCGCCTACCGGCGGGTCACCGAGACCCGCTCCAGCTTCAGCCGCGTGAGCGGTTCCCCGTCCAGCGGCTTCCGCTCGCAGTCCTGGTCCCGCGGCTCGCCCAGCACCGTGTCCTCCTCCTACAAGCGCAGCGCGCTCGCCCCGCGTCTCGCCTACAGCTCGGCTATGCTCAGCTCGGCCGAGAGCAGCCTCGACTTCAGCCAGTCCTCGTCGCTGCTCAACGGCGGCTCCGGCGGCGACTACAAACTGTCCCGCTCTAACGAGAAAGAGCAGCTGCAGGGGCTGAACGACCGCTTCGCCGGCTACATCGAGAAAGTGCACTACTTGGAACAACAGAACAAGGAGATCGAAGCAGAGATCCAGGCACTGCGGCAGAAGCAGGCCTCGCACGCCCAGCTGGGTGATGCTTACGACCAGGAGATCCGAGAGCTGCGCGCCACCCTCGAGATGGTGAACCACGAGAAGGCTCAAGTGCAGCTGGACTCCGATCACTTGGAGGAAGACATCCACCGGCTCAAGGAGCGCTTCGAGGAGGAGGCGCGGCTGCGGGACGACACCGAGGCTGCCATTCGCGCGCTGCGCAAAGACATCGAAGAGTCGTCGATGGTTAAGGTGGAGCTGGACAAGAAGGTGCAGTCGCTGCAGGATGAGGTGGCTTTCCTGCGGAGCAATCACGAAGAGGAGGTGGCCGACCTGCTGGCTCAGATCCAGGCGTCGCACATCACGGTAGAGCGCAAAGATTACCTGAAGACAGACATCTCCACGGCGCTGAAGGAGATCCGCTCCCAGCTCGAGTGTCACTCAGACCAGAACATGCACCAGGCCGAAGAGTGGTTCAAATGCCGCTACGCCAAGCTCACCGAGGCGGCCGAGCAGAACAAGGAGGCCATTCGCTCTGCCAAGGAAGAGATCGCCGAGTACCGGCGCCAGCTGCAGTCCAAGAGCATCGAGCTCGAGTCGGTGCGAGGCACTAAGGAGTCCCTGGAGCGGCAGCTCAGCGACATCGAGGAGCGCCACAACCACGACCTCAGCAGCTACCAGGACACCATCCAGCAGTTGGAAAATGAACTTCGGGGAACCAAGTGGGAAATGGCTCGTCATTTGCGAGAATACCAGGATCTCCTTAACGTCAAGATGGCCCTGGACATCGAGATCGCCGCGTACAGGAAACTCCTAGAGGGGGAAGAGACCAGATTTAGCACATTTTCAGGAAGCATCACCGGGCCTCTGTACACACACCGACAGCCCTCAGTCACAATATCCAGTAAGATTCAGAAGACCAAAGTCGAGGCCCCCAAGCTCAAGGTCCAACACAAATTTGTGGAGGAGATCATCGAAGAAACTAAAGTGGAAGATGAGAAGTCAGAAATGGAAGAAACCCTCACAGCCATCGCAGAGGAGTTGGCAGCCTCCGCCAAAGAGGAGAAGGAAGAGGCCGAAGAAAAGGAGGAGGAACCAGAAGCCGAAAAGTCTCCCGTGAAGTCTCCTGAGGCTAAGGAAGAGGAGGAGGAAGGGGAAAAGGAGGAAGAAGAGGAAGGCCAGGAGGAAGAAGAGGAGGAAGATGAAGGTGTCAAGTCAGACCAGGCAGAAGAGGGGGGATCTGAGAAGGAAGGCTCCAGTGAAAAAGATGAAGGTGAGCAGGAAGAAGAAGAAGGAGAAACCGAGGCAGAAGGTGAAGGAGAGGAAGCAGAAGCTAAGGAGGAAAAGAAAATTGAGGGAAAGGTTGAGGAAGTGGCTGTCAAGGAGGAAATCAAGGTCGAGAAGCCTGAGAAAGCCAAATCCCCTATGCCCAAATCACCCGTGGAAGAAGTAAAGCCAAAACCAGAGGCCAAGGCCGGGAAGGGTGAGCACAAGGAGGAAGAGAAAGTTGAGGAAGAGAAGAAGGAAGTCACCAAAGAATCACCCAAGGAAGAGAAGGTGGAGAAAAAGGAGGAGAAGCCAAAAGATGTTGCAGATAAAAAGAAGGCCGAGTCCCCGGTGAAAGAGAAGGCTGTGGAGGAGGTGATCACCATCAGCAAGTCGGTAAAGGTGAGCCTAGAGAAAGACACCAAAGAGGAGAAGCTGCAGCCGCAGGAGAAGGTGAAGGAGAAGGCAGAGGAGGAGGGGGGCAGTGAGGAGGAAGGGAGTGACCGTAGCCCGCAGGAGTCCAAGAAGGAAGACATAGCTATCAATGGGGAGGTGGAAGGAAAAGAGGAGGAGGAGCAGGAAACTCAGGAGAAGGGCAGTGGGCGGGAGGAGGAGAAAGGGGTGGTCACTAATGGCTTAGATGTGAGCCCTGCAGAGGAGAAGAAAGGAGAGGATAGCAGTGATGATAAAGTGGTGGTCACCAAGAAGGTAGAAAAGATCACCAGCGAGGGGGGCGATGGTGCTACCAAATACATCACCAAATCTGTAACCGTCACTCAAAAGGTTGAAGAGCATGAGGAGACCTTTGAGGAGAAGCTGGTCTCAACTAAAAAGGTAGAAAAGGTCACTTCACACGCCATAGTCAAGGAAGTCACCCAGGGTGACGctagcGCCACCatggtgagcaagggcgaggagctgttcaccggggtggtgcccatcctggtcgagctggacggcgacgtaaacggccacaagttcagcgtgtccggcgagggcgagggcgatgccacctacggcaagctgaccctgaagttcatctgcaccaccggcaagctgcccgtgccctggcccaccctcgtgaccaccctgacctacggcgtgcagtgcttcagccgctaccccgaccacatgaagcagcacgacttcttcaagtccgccatgcccgaaggctacgtccaggagcgcaccatcttcttcaaggacgacggcaactacaagacccgcgccgaggtgaagttcgagggcgacaccctggtgaaccgcatcgagctgaagggcatcgacttcaaggaggacggcaacatcctggggcacaagctggagtacaactacaacagccacaacgtctatatcatggccgacaagcagaagaacggcatcaaggtgaacttcaagatccgccacaacatcgaggacggcagcgtgcagctcgccgaccactaccagcagaacacccccatcggcgacggccccgtgctgctgcccgacaaccactacctgagcacccagtccgccctgagcaaagaccccaacgagaagcgcgatcacatggtcctgctggagttcgtgaccgccgccgggatcactctcggcatggacgagcTGTACAAGACTAGTGGCAGCGCTTGGAGtCAtCCaCAaTTtGAgAAgggcggcggcagtggcggcggcagtggcggcagtgccTGGAGCCACCCGCAGTTCGAAAAATAAggatccactagtgtcgacctcgagacctagaaaaacatggagcaatcacaagtagcaatacagcagctaccaatgctgattgtgcctggctagaagcacaagaggaggaggaggtgggttttccagtcacacctcaggtacctttaagaccaatgacttacaaggcagctgtagatcttagccactttttaaaagaaaaggggggactggaagggctaattcactcccaacgaagacaaaatcgtcgagagatgctgcatataagcagctgctttttgcttgtactgggtctctctggttagaccagatctgagcctgggagctctctggctaactagggaacccactgcttaagcctcaataaagcttgccttgagtgcttcaagtagtgtgtgcccgtctgttgtgtgactctggtaactagagatccctcagacccttttagtcagtgtggaaaatctctagcagtagtagttcatgtcatcttattattcagtatttataacttgcaaagaaatgaatatcagagagtgagaggccttgacattataatagatttagcaggaattgaactaggagtggagcacacaggcaaagctgcagaagtacttggaagaagccaccagagatactcacgattctgcacatacctggctaatcccagatcctaaggattacattaagtttactaacatttatataatgatttatagtttaaagtataaacttatctaatttactattctgacagatattaattaatcctcaaatatcataagagatgattactattatccccatttaacacaagaggaaactgagagggaaagatgttgaagtaattttcccacaattacagcatccgttagttacgactctatgatcttctgacacaaattccatttactcctcaccctatgactcagtcgaatatatcaaagttatggacattatgctaagtaacaaattacccttttatatagtaaatactgagtagattgagagaagaaattgtttgcaaacctgaatagcttcaagaagaagagaagtgaggataagaataacagttgtcatttaacaagttttaacaagtaacttggttagaaagggattcaaatgcataaagcaagggataaatttttctggcaacaagactatacaatataaccttaaatatgacttcaaataattgttggaacttgataaaactaattaaatattattgaagattatcaatattataaatgtaatttacttttaaaaagggaacatagaaatgtgtatcattagagtagaaaacaatccttattatcacaatttgtcaaaacaagtttgttattaacacaagtagaatactgcattcaattaagttgactgcagattttgtgttttgttaaaattagaaagagataacaacaatttgaattattgaaagtaacatgtaaatagttctacatacgttcttttgacatcttgttcaatcattgatcgaagttctttatcttggaagaatttgttccaaagactctgaaataaggaaaacaatctattatatagtctcacacctttgttttacttttagtgatttcaatttaataatgtaaatggttaaaatttattcttctctgagatcatttcacattgcagatagaaaacctgagactggggtaatttttattaaaatctaattt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Annotated sequence file provided by depositor
