EPR Plasmid #12: pMT3b-REPTOR-HA
Plasmid Details
| Purpose | Copper-inducible expression of HA-tagged Drosophila REPTOR in fly cell culture |
| Depositing Lab |
Aurelio Teleman Deutsches Krebsforschungszentrum (DKFZ) |
| Depositing Scientist | Aurelio Teleman |
| Antibiotic Resistance | Ampicillin |
| Bacterial Growth Temp | 37 |
| Copy Number | High |
| Selectable Marker | |
| Reference |
REPTOR and REPTOR-BP Regulate Organismal Metabolism and Transcription Downstream of TORC1. Tiebe M et al. Dev Cell 2015 PubMed DOI See other plasmids from this publication |
| MTA and Terms |
MTA (PDF download) |
How to Cite This Reagent
IN THE MATERIALS & METHODS:
pMT3b-REPTOR-HA was a gift from Aurelio Teleman (European Plasmid Repository plasmid #12).
pMT3b-REPTOR-HA was a gift from Aurelio Teleman (European Plasmid Repository plasmid #12).
IN THE REFERENCES:
Tiebe M et al.. REPTOR and REPTOR-BP Regulate Organismal Metabolism and Transcription Downstream of TORC1.. Dev Cell 2015 doi:10.1016/j.devcel.2015.03.013 PubMed 25920570
Tiebe M et al.. REPTOR and REPTOR-BP Regulate Organismal Metabolism and Transcription Downstream of TORC1.. Dev Cell 2015 doi:10.1016/j.devcel.2015.03.013 PubMed 25920570
Sequences
Provided by depositor
download sequence
ggcactggccgtcgttttacaacgtcgtgactgggaaaaccctggcgttacccaacttaatcgccttgcagcacatccccctttcgccagctggcgtaatagcgaagaggcccgcaccgatcgcccttcccaacagttgcgcagcctgaatggcgaatggcgcctgatgcggtattttctccttacgcatctgtgcggtatttcacaccgcatatggtgcactctcagtacaatctgctctgatgccgcatagttaagccagccccgacacccgccaacacccgctgacgcgccctgacgggcttgtctgctcccggcatccgcttacagacaagctgtgaccgtctccgggagctgcatgtgtcagaggttttcaccgtcatcaccgaaacgcgcgagacgaaagggcctcgtgatacgcctatttttataggttaatgtcatgataataatggtttcttagacgtcaggtggcacttttcggggaaatgtgcgcggaacccctatttgtttatttttctaaatacattcaaatatgtatccgctcatgagacaataaccctgataaatgcttcaataatattgaaaaaggaagagtatgagtattcaacatttccgtgtcgcccttattcccttttttgcggcattttgccttcctgtttttgctcacccagaaacgctggtgaaagtaaaagatgctgaagatcagttgggtgcacgagtgggttacatcgaactggatctcaacagcggtaagatccttgagagttttcgccccgaagaacgttttccaatgatgagcacttttaaagttctgctatgtggcgcggtattatcccgtattgacgccgggcaagagcaactcggtcgccgcatacactattctcagaatgacttggttgagtactcaccagtcacagaaaagcatcttacggatggcatgacagtaagagaattatgcagtgctgccataaccatgagtgataacactgcggccaacttacttctgacaacgatcggaggaccgaaggagctaaccgcttttttgcacaacatgggggatcatgtaactcgccttgatcgttgggaaccggagctgaatgaagccataccaaacgacgagcgtgacaccacgatgcctgtagcaatggcaacaacgttgcgcaaactattaactggcgaactacttactctagcttcccggcaacaattaatagactggatggaggcggataaagttgcaggaccacttctgcgctcggcccttccggctggctggtttattgctgataaatctggagccggtgagcgtgggtctcgcggtatcattgcagcactggggccagatggtaagccctcccgtatcgtagttatctacacgacggggagtcaggcaactatggatgaacgaaatagacagatcgctgagataggtgcctcactgattaagcattggtaactgtcagaccaagtttactcatatatactttagattgatttaaaacttcatttttaatttaaaaggatctaggtgaagatcctttttgataatctcatgaccaaaatcccttaacgtgagttttcgttccactgagcgtcagaccccgtagaaaagatcaaaggatcttcttgagatcctttttttctgcgcgtaatctgctgcttgcaaacaaaaaaaccaccgctaccagcggtggtttgtttgccggatcaagagctaccaactctttttccgaaggtaactggcttcagcagagcgcagataccaaatactgtccttctagtgtagccgtagttaggccaccacttcaagaactctgtagcaccgcctacatacctcgctctgctaatcctgttaccagtggctgctgccagtggcgataagtcgtgtcttaccgggttggactcaagacgatagttaccggataaggcgcagcggtcgggctgaacggggggttcgtgcacacagcccagcttggagcgaacgacctacaccgaactgagatacctacagcgtgagctatgagaaagcgccacgcttcccgaagggagaaaggcggacaggtatccggtaagcggcagggtcggaacaggagagcgcacgagggagcttccagggggaaacgcctggtatctttatagtcctgtcgggtttcgccacctctgacttgagcgtcgatttttgtgatgctcgtcaggggggcggagcctatggaaaaacgccagcaacgcggcctttttacggttcctggccttttgctggccttttgctcacatgttctttcctgcgttatcccctgattctgtggataaccgtattaccgcctttgagtgagctgataccgctcgccgcagccgaacgaccgagcgcagcgagtcagtgagcgaggaagcggaagagcgcccaatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggctcgtatgttgtgtggaattgtgagcggataacaatttcacacaggaaacagctatgacatgattaccgttgcaggacaggatgtggtgcccgatgtgactagctctttgctgcaggccgtcctatcctctggttccgataagagacccagaactccggccccccaccgcccaccgccacccccatacatatgtggtacgcaagtaagagtgcctgcgcatgccccatgtgccccaccaagagttttgcatcccatacaagtccccaaagtggagaaccgaaccaattcttcgcgggcagaacaaaagcttctgcacacgtctccactcgaatttggagccggccggcgtgtgcaaaagaggtgaatcgaacgaaagacccgtgtgtaaagccgcgtttccaaaatgtataaaaccgagagcatctggccaatgtgcatcagttgtggtcagcagcaaaatcaagtgaatcatctcagtgcaactaaagggaattcATGACAGAGAATCAGCTGTATCCCATGTCATCGGAGTTCTTTGACACCGGCTCGAACAGCAGCAGCAACACACTTAAATACGATCTGTATTCCCTGGGCTCCACCGTTGTGGGGGCGGCGCTGCCCACGTTGACGATCAACACGGGCTACGGCAGCAGCAGCAGCAACAATAACAACACTAATAACAACAATAACAACAGCAGTAGTCACAGCAGCAGCAGCAACTGCTCCACCAGCACCACAAATACCTGTAATGTAATGCTAAGTACAACGGCCATAACAATACCACGCAGCAGCAACCACAACCAGATCCATCATCATCCGTATCAGCAGTCCGATTTGCAGACACCGCGCCATCATCCCAGTCCTCTACACACGTTGCCCAGCATGACCCATCAGCAAAATCAGCTTCAGCAGCAGCAGCAGCAGCAACACCACAACCAACAGCAGCAGCTGCAGCAATCGCATTTGGCACTGGGAGAGCTTTCAGACTTTGGACTAGATGCACTGGATGCCGCTTCGCTATCGCCCACTCTGCTGCAGGATGTGAGCCTGAGTGCTGTTTCTCCTCTAAGCACCACACTCTACAATGGCAACACCAGTGGAGCAGGCAGCAGCAACGGAATAGGATCCGGCAGTGGTGGCTACTTCACGCCCGACATGTCGCACAGCCTCAGCCTGAATGTGGTCAGTGAGCAGGTTCTGCTGCAGGAAGCCACAACCCCGAATGAGTTGCTCTACGAGATGACACCCAACTCGAATGCCATGTGGAGTGATATCAGCAGTGCCATTATCCATACCAAACACGAGCCATTCAGTCTGGACGATGACTATATCTTTCCCAACGATAAGGCCGAAATACAGGCAGCCGATTTGTCTGACCTTAATGGCGGCGATTTTCTGGATGTGATCGGAAATATAGAGGATTTCTTGCCGCAGACCGCAGTCACTCAAAGTGTCAATTTCCTACTGTCGCCGCAAGCTCAAGGACAGGATGCCCTGGTGGCACCGCCCATGGAACTTTTGCAACAGCAACAACAGAATCACCAGCAGCTGCAGGTCGGCAGCCTGCCGCAGTTGCAAACGCTGCTCACGCTTTCCCAGCAGCAGCAGTCGAACAGTTCCTCCACTAGTCCGTATGAGATCTATCACAGTACGCCCCAAAAGCCGCAGCAGCAACAGCTGTCCGCCAGCTTCTCGCCCGGCAGTCAGGCATCCCAATCGCCATTGACCCCACCGCCGCCACCGCACGCCAATCGACCCCAGTATCAGATGGTTAAGTCGCGCAACATGCAGGAATTGATCAAGAAGGGCTTTCCCATGTCCTCGCCGCCAGAACGCTCGATCCTAAGTCAGTCCGCCGCCTTGAGTCCGGGTGGTAGCAGTGGTTTTGGCAGCTCGGCTTCTGGCAACAGTACAACTACCAGTAATCAGACATCCGGATCAGCCGTGCGCAAATCGTTTGGCTACCAGAGCGCCGTTGAGAACTCCCAGCTGAGTCGCCTCAGCTCGTCGGCTCCGACGCACTTGGGATTGGAGCACATCTGGATGCGGCGGGAGCCACGACAGCATTTACTCTCCACCGGCTCGCTGGCCGAGGCCGAGAGCTTCTCTTCACTGAGCACGGGCAGTGTCTTGTCCCCGGATGGCATTGACTTCTCGCAGGACGATGAGGATGACAATTCGAGTGAGAATAGCGATAACTACGACGATTGTAGCAGCGACAATGGACTCTCTGAGGATGAAGATGAAACGCGTACCTCGACACCAAACCATTTGTCCAGTAGCAAGGGCAAAGAGCGATTTTTCTGGCAGTACAATGTTCAGGCCAAAGGACCCAAGGGAAAGAGACTGGTCTTTCAATCGAAGCTCGAGGATCCTCATGTGCTGAACGAGGTGACAGACCCCGTCTTCAGTCCAACGTGCTCCGTGCGAGGCATTAAGGTGTACAAGCACAGTGGCAAGGCCCGCAAGGGCGATGGTAACGATTTAACTCCCAATGCCAGGAAGCTGCACAATATTGGCAAGGAACTGGACAAGCTAAGCCGCACCATCAATGACATGACTCCTGTTTCCGAGCTGCCATTCAATGTGCGCCCCAAGTCGCGCAAGGAGAAGAACAAGCTGGCCTCACGCGCCTGTCGCCTGAAGAAGAAGGCGCAGCACGAGGCCAACAAAATCAAGTTGTTCGGCCTTGAAATTGAACACAAGCGCCTCATGAATGGTATAGCCGAGCTAAAGCAGGCCTTGGTCGTCAAGCATCGCACCAAGAACCTGGGCGAATCCACCGAGGAGGTGGACCAACAAATTGCACGCATTTACGCTACCGCCTCTTCTGGAATCCGCATTGCGGGTGGTTCAACGGATTTTGTGAACAAGGTGCTGGAGAATATGCGCGGCGGCATGCCGAATGGAGGCCTCGAGGAGCTGCGTAAATCGTCGggatcctacccctacgacgtgcccgactacgcctaagtcgacctgcaggcatgcaagctattcgatgcacactcacattcttctcctaatacgataataaaactttccatgaaaaatatggaaaaatatatgaaaattgagaaatccaaaaaactgataaacgctctacttaattaaaatagataaatgggagcggcaggaatggcggagcatggccaagttcctccgccaatcagtcgtaaaacagaagtcgtggaaagcggatagaaagaatgttcgatttgacgggcaagcatgtctgctatgtggcggattgcggaggaattgcactggagaccagcaaggttctcatgaccaagaatatagcggtgagtgagcgggaagctcggtttctgtccagatcgaactcaaaactagtccagccagtcgctgtcgaaactaattaagtaaatgagtttttcatgttagtttcgcgctgagcaacaattaagtttatgtttcagttcggcttagatttcgctgaaggacttgccactttcaatcaatactttagaacaaaatcaaaactcattctaatagcttggtgttcatctttttttttaatgataagcattttgtcgtttatactttttatatttcgatattaaaccacctatgaagttcattttaatcgccagataagcaatatattgtgtaaatatttgtattctttatcaggaaattcagggagacggggaagttactatctactaaaagccaaacaatttcttacagttttactctctctactctagagtagctt
ggcactggccgtcgttttacaacgtcgtgactgggaaaaccctggcgttacccaacttaatcgccttgcagcacatccccctttcgccagctggcgtaatagcgaagaggcccgcaccgatcgcccttcccaacagttgcgcagcctgaatggcgaatggcgcctgatgcggtattttctccttacgcatctgtgcggtatttcacaccgcatatggtgcactctcagtacaatctgctctgatgccgcatagttaagccagccccgacacccgccaacacccgctgacgcgccctgacgggcttgtctgctcccggcatccgcttacagacaagctgtgaccgtctccgggagctgcatgtgtcagaggttttcaccgtcatcaccgaaacgcgcgagacgaaagggcctcgtgatacgcctatttttataggttaatgtcatgataataatggtttcttagacgtcaggtggcacttttcggggaaatgtgcgcggaacccctatttgtttatttttctaaatacattcaaatatgtatccgctcatgagacaataaccctgataaatgcttcaataatattgaaaaaggaagagtatgagtattcaacatttccgtgtcgcccttattcccttttttgcggcattttgccttcctgtttttgctcacccagaaacgctggtgaaagtaaaagatgctgaagatcagttgggtgcacgagtgggttacatcgaactggatctcaacagcggtaagatccttgagagttttcgccccgaagaacgttttccaatgatgagcacttttaaagttctgctatgtggcgcggtattatcccgtattgacgccgggcaagagcaactcggtcgccgcatacactattctcagaatgacttggttgagtactcaccagtcacagaaaagcatcttacggatggcatgacagtaagagaattatgcagtgctgccataaccatgagtgataacactgcggccaacttacttctgacaacgatcggaggaccgaaggagctaaccgcttttttgcacaacatgggggatcatgtaactcgccttgatcgttgggaaccggagctgaatgaagccataccaaacgacgagcgtgacaccacgatgcctgtagcaatggcaacaacgttgcgcaaactattaactggcgaactacttactctagcttcccggcaacaattaatagactggatggaggcggataaagttgcaggaccacttctgcgctcggcccttccggctggctggtttattgctgataaatctggagccggtgagcgtgggtctcgcggtatcattgcagcactggggccagatggtaagccctcccgtatcgtagttatctacacgacggggagtcaggcaactatggatgaacgaaatagacagatcgctgagataggtgcctcactgattaagcattggtaactgtcagaccaagtttactcatatatactttagattgatttaaaacttcatttttaatttaaaaggatctaggtgaagatcctttttgataatctcatgaccaaaatcccttaacgtgagttttcgttccactgagcgtcagaccccgtagaaaagatcaaaggatcttcttgagatcctttttttctgcgcgtaatctgctgcttgcaaacaaaaaaaccaccgctaccagcggtggtttgtttgccggatcaagagctaccaactctttttccgaaggtaactggcttcagcagagcgcagataccaaatactgtccttctagtgtagccgtagttaggccaccacttcaagaactctgtagcaccgcctacatacctcgctctgctaatcctgttaccagtggctgctgccagtggcgataagtcgtgtcttaccgggttggactcaagacgatagttaccggataaggcgcagcggtcgggctgaacggggggttcgtgcacacagcccagcttggagcgaacgacctacaccgaactgagatacctacagcgtgagctatgagaaagcgccacgcttcccgaagggagaaaggcggacaggtatccggtaagcggcagggtcggaacaggagagcgcacgagggagcttccagggggaaacgcctggtatctttatagtcctgtcgggtttcgccacctctgacttgagcgtcgatttttgtgatgctcgtcaggggggcggagcctatggaaaaacgccagcaacgcggcctttttacggttcctggccttttgctggccttttgctcacatgttctttcctgcgttatcccctgattctgtggataaccgtattaccgcctttgagtgagctgataccgctcgccgcagccgaacgaccgagcgcagcgagtcagtgagcgaggaagcggaagagcgcccaatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggctcgtatgttgtgtggaattgtgagcggataacaatttcacacaggaaacagctatgacatgattaccgttgcaggacaggatgtggtgcccgatgtgactagctctttgctgcaggccgtcctatcctctggttccgataagagacccagaactccggccccccaccgcccaccgccacccccatacatatgtggtacgcaagtaagagtgcctgcgcatgccccatgtgccccaccaagagttttgcatcccatacaagtccccaaagtggagaaccgaaccaattcttcgcgggcagaacaaaagcttctgcacacgtctccactcgaatttggagccggccggcgtgtgcaaaagaggtgaatcgaacgaaagacccgtgtgtaaagccgcgtttccaaaatgtataaaaccgagagcatctggccaatgtgcatcagttgtggtcagcagcaaaatcaagtgaatcatctcagtgcaactaaagggaattcATGACAGAGAATCAGCTGTATCCCATGTCATCGGAGTTCTTTGACACCGGCTCGAACAGCAGCAGCAACACACTTAAATACGATCTGTATTCCCTGGGCTCCACCGTTGTGGGGGCGGCGCTGCCCACGTTGACGATCAACACGGGCTACGGCAGCAGCAGCAGCAACAATAACAACACTAATAACAACAATAACAACAGCAGTAGTCACAGCAGCAGCAGCAACTGCTCCACCAGCACCACAAATACCTGTAATGTAATGCTAAGTACAACGGCCATAACAATACCACGCAGCAGCAACCACAACCAGATCCATCATCATCCGTATCAGCAGTCCGATTTGCAGACACCGCGCCATCATCCCAGTCCTCTACACACGTTGCCCAGCATGACCCATCAGCAAAATCAGCTTCAGCAGCAGCAGCAGCAGCAACACCACAACCAACAGCAGCAGCTGCAGCAATCGCATTTGGCACTGGGAGAGCTTTCAGACTTTGGACTAGATGCACTGGATGCCGCTTCGCTATCGCCCACTCTGCTGCAGGATGTGAGCCTGAGTGCTGTTTCTCCTCTAAGCACCACACTCTACAATGGCAACACCAGTGGAGCAGGCAGCAGCAACGGAATAGGATCCGGCAGTGGTGGCTACTTCACGCCCGACATGTCGCACAGCCTCAGCCTGAATGTGGTCAGTGAGCAGGTTCTGCTGCAGGAAGCCACAACCCCGAATGAGTTGCTCTACGAGATGACACCCAACTCGAATGCCATGTGGAGTGATATCAGCAGTGCCATTATCCATACCAAACACGAGCCATTCAGTCTGGACGATGACTATATCTTTCCCAACGATAAGGCCGAAATACAGGCAGCCGATTTGTCTGACCTTAATGGCGGCGATTTTCTGGATGTGATCGGAAATATAGAGGATTTCTTGCCGCAGACCGCAGTCACTCAAAGTGTCAATTTCCTACTGTCGCCGCAAGCTCAAGGACAGGATGCCCTGGTGGCACCGCCCATGGAACTTTTGCAACAGCAACAACAGAATCACCAGCAGCTGCAGGTCGGCAGCCTGCCGCAGTTGCAAACGCTGCTCACGCTTTCCCAGCAGCAGCAGTCGAACAGTTCCTCCACTAGTCCGTATGAGATCTATCACAGTACGCCCCAAAAGCCGCAGCAGCAACAGCTGTCCGCCAGCTTCTCGCCCGGCAGTCAGGCATCCCAATCGCCATTGACCCCACCGCCGCCACCGCACGCCAATCGACCCCAGTATCAGATGGTTAAGTCGCGCAACATGCAGGAATTGATCAAGAAGGGCTTTCCCATGTCCTCGCCGCCAGAACGCTCGATCCTAAGTCAGTCCGCCGCCTTGAGTCCGGGTGGTAGCAGTGGTTTTGGCAGCTCGGCTTCTGGCAACAGTACAACTACCAGTAATCAGACATCCGGATCAGCCGTGCGCAAATCGTTTGGCTACCAGAGCGCCGTTGAGAACTCCCAGCTGAGTCGCCTCAGCTCGTCGGCTCCGACGCACTTGGGATTGGAGCACATCTGGATGCGGCGGGAGCCACGACAGCATTTACTCTCCACCGGCTCGCTGGCCGAGGCCGAGAGCTTCTCTTCACTGAGCACGGGCAGTGTCTTGTCCCCGGATGGCATTGACTTCTCGCAGGACGATGAGGATGACAATTCGAGTGAGAATAGCGATAACTACGACGATTGTAGCAGCGACAATGGACTCTCTGAGGATGAAGATGAAACGCGTACCTCGACACCAAACCATTTGTCCAGTAGCAAGGGCAAAGAGCGATTTTTCTGGCAGTACAATGTTCAGGCCAAAGGACCCAAGGGAAAGAGACTGGTCTTTCAATCGAAGCTCGAGGATCCTCATGTGCTGAACGAGGTGACAGACCCCGTCTTCAGTCCAACGTGCTCCGTGCGAGGCATTAAGGTGTACAAGCACAGTGGCAAGGCCCGCAAGGGCGATGGTAACGATTTAACTCCCAATGCCAGGAAGCTGCACAATATTGGCAAGGAACTGGACAAGCTAAGCCGCACCATCAATGACATGACTCCTGTTTCCGAGCTGCCATTCAATGTGCGCCCCAAGTCGCGCAAGGAGAAGAACAAGCTGGCCTCACGCGCCTGTCGCCTGAAGAAGAAGGCGCAGCACGAGGCCAACAAAATCAAGTTGTTCGGCCTTGAAATTGAACACAAGCGCCTCATGAATGGTATAGCCGAGCTAAAGCAGGCCTTGGTCGTCAAGCATCGCACCAAGAACCTGGGCGAATCCACCGAGGAGGTGGACCAACAAATTGCACGCATTTACGCTACCGCCTCTTCTGGAATCCGCATTGCGGGTGGTTCAACGGATTTTGTGAACAAGGTGCTGGAGAATATGCGCGGCGGCATGCCGAATGGAGGCCTCGAGGAGCTGCGTAAATCGTCGggatcctacccctacgacgtgcccgactacgcctaagtcgacctgcaggcatgcaagctattcgatgcacactcacattcttctcctaatacgataataaaactttccatgaaaaatatggaaaaatatatgaaaattgagaaatccaaaaaactgataaacgctctacttaattaaaatagataaatgggagcggcaggaatggcggagcatggccaagttcctccgccaatcagtcgtaaaacagaagtcgtggaaagcggatagaaagaatgttcgatttgacgggcaagcatgtctgctatgtggcggattgcggaggaattgcactggagaccagcaaggttctcatgaccaagaatatagcggtgagtgagcgggaagctcggtttctgtccagatcgaactcaaaactagtccagccagtcgctgtcgaaactaattaagtaaatgagtttttcatgttagtttcgcgctgagcaacaattaagtttatgtttcagttcggcttagatttcgctgaaggacttgccactttcaatcaatactttagaacaaaatcaaaactcattctaatagcttggtgttcatctttttttttaatgataagcattttgtcgtttatactttttatatttcgatattaaaccacctatgaagttcattttaatcgccagataagcaatatattgtgtaaatatttgtattctttatcaggaaattcagggagacggggaagttactatctactaaaagccaaacaatttcttacagttttactctctctactctagagtagctt
