EPR Plasmid #184: pcDNA3-Flag-TSC22D4 (wild type, mus musculus)
Plasmid Details
| Purpose | Overexpression of Flag tagged TSC22D4 cDNA under the CMV promoter. (NCBI Reference Sequence: NM_023910.7) |
| Depositing Lab |
Bilgen Ekim Üstünel Helmholtz Center for Environmental Health |
| Depositing Scientist | Bilgen Ekim Üstünel |
| Antibiotic Resistance | Ampicillin |
| Bacterial Growth Temp | 37 |
| Copy Number | High |
| Selectable Marker | |
| Reference |
TSC22D4 interacts with Akt1 in response to metabolic and stress signals Demir, S.; Wolff, G.; Wieder, A.; Maida, A.; Rahm, M.; Schnoelzer, M.; Hauck, S.; Szendroedi, J.; H... DOI See other plasmids from this preprint |
| MTA and Terms |
MTA (PDF download) |
How to Cite This Reagent
IN THE MATERIALS & METHODS:
pcDNA3-Flag-TSC22D4 (wild type, mus musculus) was a gift from Bilgen Ekim Üstünel (European Plasmid Repository plasmid #184).
pcDNA3-Flag-TSC22D4 (wild type, mus musculus) was a gift from Bilgen Ekim Üstünel (European Plasmid Repository plasmid #184).
IN THE REFERENCES:
Demir, S.; Wolff, G.; Wieder, A.; Maida, A.; Rahm, M.; Schnoelzer, M.; Hauck, S.; Szendroedi, J.; H.... TSC22D4 interacts with Akt1 in response to metabolic and stress signals . doi: 10.1101/2021.12.20.473554
Demir, S.; Wolff, G.; Wieder, A.; Maida, A.; Rahm, M.; Schnoelzer, M.; Hauck, S.; Szendroedi, J.; H.... TSC22D4 interacts with Akt1 in response to metabolic and stress signals . doi: 10.1101/2021.12.20.473554
Sequences
Provided by depositor
download sequence
insert sequence: CTCTCTGGCTACTAGAGAACCCACTGCTTACTGGCTTATCGAAATTAATACGACTCACTATAGGGAGACCCAAGCTTGGTACCGAGCTCGGATCCACTAGTAACGGCCGCCAGTGTGCTGGAATTCTGCCACCATGGACTACAAGGACGACGATGACAAGCCAGCGGCCGCGAGTGGTGGTAAGAAGAAAAGTAGTTTTCAGATCACCAGCGTCACCACGGACTATGAGGGCCCAGGAAGCCCAGGGGCTTCGGATTCCCCTGTCCCACCGGCTCTCGCTGGGCCCCCGCCCCGCCTCCC CAATGGGGACCCCAACCCTGATCCAGGGGGCAGGGGCACCCCCCGGAATGGCTCTCCGCCACCTGGAGCCCCTGCCTCCCGTTTCCGGGTGGTGAAGCTGCCCCAAGGTTTGGGAGAGCCTTATCGTCGAGGTCGGTGGACGTGTGTGGATGTTTACGAGAGAGATCTGGAGCCTCCAAGCTTCGGTCGGCTTCTGGAGGGCATTCGAGGGGCCTCTGGAGGCACCGGAGGCAGATCATTGGATTCTAGGTTGGAGCTGGCTAGTTTGGGCATAAGCACCCCTATCCCGCAGCCAGGCCT GTCTCAGGGCCCCACCTCTTGGCTCCGCCCGCCCCCTACTTCTCCTGGACCACAGGCCCGCTCCTTCACAGGGGGGCTGGGCCAGCTGGCAGGACCTGGCAAGGCTAAGGTGGAGACACCCCCGCTGTCAGCATCTCCACCCCAGCAGCGCCCCCCAGGGCCTGGGACTGGAGATAGTGCTCAGACCCTGCCCTCTCTGAGGGTAGAAGTGGAGTCTGGGGGTTCAGCAGCCGCAACCCCTCCACTGTCACGGAGAAGAGATGGAGCAGTTCGGCTGAGGATGGAGTTAGTTGCTCCAGC GGAGACAGGAAAGGTACCTCCCACCGACTCTCGCCCCAACTCCCCAGCCCTCTACTTCGATGCCAGCCTGGTCCATAAGTCTCCAGACCCATTTGGAGCCGCAGCAGCCCAGAGCCTCAGCCTGGCCCGTTCCATGTTGGCTATCAGCGG TCACCTGGACAGCGATGACGATAGTGGTTCCGGAAGCCTGGTTGGCATTGACAACAAGATTGAACAAGCCATGGACTTGGTGAAGTCCCACCTCATGTTTGCCGTGCGAGAAGAGGTGGAGGTGCTGAAGGAGCAGATCCGAGACCTGGCAGAGCGGAACGCTGCATTGGAGCAGGAGAATGGATTGCTGCGCGCCCTGGCCAGCCCGGAGCAGCTGGCCCAGCTGCCATCCTCGGGGCTCCCCAGGCTCGGGCCCTCTGCACCCAATGGGCCCTCCATCTGACTCGAGGGGGGGCCC
insert sequence: CTCTCTGGCTACTAGAGAACCCACTGCTTACTGGCTTATCGAAATTAATACGACTCACTATAGGGAGACCCAAGCTTGGTACCGAGCTCGGATCCACTAGTAACGGCCGCCAGTGTGCTGGAATTCTGCCACCATGGACTACAAGGACGACGATGACAAGCCAGCGGCCGCGAGTGGTGGTAAGAAGAAAAGTAGTTTTCAGATCACCAGCGTCACCACGGACTATGAGGGCCCAGGAAGCCCAGGGGCTTCGGATTCCCCTGTCCCACCGGCTCTCGCTGGGCCCCCGCCCCGCCTCCC CAATGGGGACCCCAACCCTGATCCAGGGGGCAGGGGCACCCCCCGGAATGGCTCTCCGCCACCTGGAGCCCCTGCCTCCCGTTTCCGGGTGGTGAAGCTGCCCCAAGGTTTGGGAGAGCCTTATCGTCGAGGTCGGTGGACGTGTGTGGATGTTTACGAGAGAGATCTGGAGCCTCCAAGCTTCGGTCGGCTTCTGGAGGGCATTCGAGGGGCCTCTGGAGGCACCGGAGGCAGATCATTGGATTCTAGGTTGGAGCTGGCTAGTTTGGGCATAAGCACCCCTATCCCGCAGCCAGGCCT GTCTCAGGGCCCCACCTCTTGGCTCCGCCCGCCCCCTACTTCTCCTGGACCACAGGCCCGCTCCTTCACAGGGGGGCTGGGCCAGCTGGCAGGACCTGGCAAGGCTAAGGTGGAGACACCCCCGCTGTCAGCATCTCCACCCCAGCAGCGCCCCCCAGGGCCTGGGACTGGAGATAGTGCTCAGACCCTGCCCTCTCTGAGGGTAGAAGTGGAGTCTGGGGGTTCAGCAGCCGCAACCCCTCCACTGTCACGGAGAAGAGATGGAGCAGTTCGGCTGAGGATGGAGTTAGTTGCTCCAGC GGAGACAGGAAAGGTACCTCCCACCGACTCTCGCCCCAACTCCCCAGCCCTCTACTTCGATGCCAGCCTGGTCCATAAGTCTCCAGACCCATTTGGAGCCGCAGCAGCCCAGAGCCTCAGCCTGGCCCGTTCCATGTTGGCTATCAGCGG TCACCTGGACAGCGATGACGATAGTGGTTCCGGAAGCCTGGTTGGCATTGACAACAAGATTGAACAAGCCATGGACTTGGTGAAGTCCCACCTCATGTTTGCCGTGCGAGAAGAGGTGGAGGTGCTGAAGGAGCAGATCCGAGACCTGGCAGAGCGGAACGCTGCATTGGAGCAGGAGAATGGATTGCTGCGCGCCCTGGCCAGCCCGGAGCAGCTGGCCCAGCTGCCATCCTCGGGGCTCCCCAGGCTCGGGCCCTCTGCACCCAATGGGCCCTCCATCTGACTCGAGGGGGGGCCC
