EPR Plasmid #189: pHD918
Sequence: download sequence
| Purpose of plasmid | Inducible expression of tandem-affinity-tagged protein in Trypanosoma brucei. IgG binding domain - TEV- calmodulin binging peptide from pBS1479 (Seraphin).. Cut with Not I to transfect.. Polylinker for ORF. |
| Depositing Lab |
Christine Clayton Heidelberg University |
| Depositing Scientist | Christine Clayton |
| Antibiotic Resistance | Ampicillin |
| Bacterial Growth Temp | 37 |
| Copy Number | High |
| Selectable Marker | Hygromycin |
| Reference |
The exosome of Trypanosoma brucei. Estévez AM et al. EMBO J 2001 PubMed |
| MTA and Terms |
MTA (PDF download) |
How to cite this reagent:
In the Materials & Methods:
pHD918 was a gift from Christine Clayton (European Plasmid Repository plasmid #189).
In the References:
Estévez AM et al.. The exosome of Trypanosoma brucei.. EMBO J 2001 PubMed 11447124
pHD918 was a gift from Christine Clayton (European Plasmid Repository plasmid #189).
In the References:
Estévez AM et al.. The exosome of Trypanosoma brucei.. EMBO J 2001 PubMed 11447124
Sequence: download sequence
gaattcgagctcatatagttggtatgtattctaattccagactactggcgtgggataaacatgtccccctgattaaaaggaaagattccatagcccattagtgcaaagataattggtacaccgtcaaaaacacatgggagaccacacgatacgacactgtgaccgtagcatcaattccgcactcgatataactctatcgaagaacttccatggtacaaactggtcgccacggtctgctccacacggagatcatcgtatcatttttatcgatagcggccgctatcgatgtatgccttggccctgatggcatgccaatttcactacaacggactatgtggaccccgttatcatggaaatgcgctagttggaggaagttagaccgcgccggaaaagagaggggtagagaaaatgacaacttggaagatatccacacacgcacggtgaaacgttagcaacaattattagggaagcacgcttgccctagtcccacgagtaaccaagactccaaaagcctttctggcacagagagcgagccgaaatggaaaagagaaacaatgcctgcactaacactactgagcgattcgcctcgccgcggaggaccgaatactaataacgacacttgcggtcaaaaagtagaagaacaaatgctcaacgatgagtgaatcaggttagggtagttggaaaattatacagaatgtctttggcaacacaccggtaccgtcattggggttaagcggaaaggtgtgtgtcagtaggttgtgaggtgaaagcgttttcagatgcatagtgagcttaatgtccttttcacagtatatcgtgtctgataggtatctcttattagtatagtcgaatactagtcaatagtgcgttttgtgcaaaatgtccattttgtggcagtgatggggttgttttatgctattccgtgtctctgggtgggcgtgcattgaaaataggggttatcgggtagggatctccctatcagtgatagagatctccctatcagtgatagagatccctgagtactgagtttaacatgttctcgtcccgggctgcacgcgccttcgagttttttttccttttccccatttttttcaacttgaagacttcaattacaccaaaaagtaaaattcacaagcttcgcggggcccacgcgttctagagttaacatggaaaagcgacgatggaaaaagaatttcatagccgtctcagcagccaaccgctttaagaaaatctcatcctccggggcacttgattatgatattccaactactgctagcgagaatttgtattttcagggtgagctcaaaaccgcggctcttgcgcaacacgatgaagccgtggacaacaaattcaacaaagaacaacaaaacgcgttctatgagatcttacatttacctaacttaaacgaagaacaacgaaacgccttcatccaaagtttaaaagatgacccaagccaaagcgctaaccttttagcagaagctaaaaagctaaatgatgctcaggcgccgaaagtagacaacaaattcaacaaagaacaacaaaacgcgttctatgagatcttacatttacctaacttaaacgaagaacaacgaaacgccttcatccaaagtttaaaagatgacccaagccaaagcgctaaccttttagcagaagctaaaaagctaaatggtgctcaggcgccgaaagtagacgcgaattccgcggggaagtcaacctaaggatcctaacaccgggttgtgtggccaaaattgttctgtagtcgctgtgagttgacacggctagtgcttatgattttcctcgcgtgtggtgcctgtactcagccctatgccttatttgcaacacatttacgtacagcgcacaagagaagagaagatcacttgaagataataaatatagggttgtaggcatcttgtttaactcaaattttctcgtcttggtgtgtcgacatgattgaaatagtgccaccagttgtgtttgatgcgtttgttatctatgcagtattctgcagtttaatttgttggatgagctatttcattaatttttttgccttctcttcttttgggttcgctcgagagagagagggtgggatttttttctttaaataaagggagatgtattcggaggagttgcattaacgttgtcccatgttttacaaaaaacgagtctgaaagaacgccgactcaatgcccatcaagcggaataaccttctaaaagaatcatatccctattaccacaccagtttatattacaggggaggttattacagaaatctaggatatcagactcaaggtgtggcggacgtctcgaaccgatcgtgagttggcccgggcacagcaaggtcttctgaaattcatgttttttttttttttactctgcattgcagtctccgctcttatttagttttgctttacgtaaggtctcgttgctgccataaaataagctctagaactagtgatgaaaaagcctgaactcaccgcgacgtctgtcgagaagtttctgatcgaaaagttcgacagcgtctccgacctgatgcagctctcggagggcgaagaatctcgtgctttcagcttcgatgtaggagggcgtggatatgtcctgcgggtaaatagctgcgccgatggtttctacaaagatcgttatgtttatcggcactttgcatcggccgcgctcccgattccggaagtgcttgacattggggaattcagcgagagcctgacctattgcatctcccgccgtgcacagggtgtcacgttgcaagacctgcctgaaaccgaactgcccgctgttctgcagccggtcgcggaggccatggatgcgatcgctgcggccgatcttagccagacgagcgggttcggcccattcggaccgcaaggaatcggtcaatacactacatggcgtgatttcatatgcgcgattgctgatccccatgtgtatcactggcaaactgtgatggacgacaccgtcagtgcgtccgtcgcgcaggctctcgatgagctgatgctttgggccgaggactgccccgaagtccggcacctcgtgcacgcggatttcggctccaacaatgtcctgacggacaatggccgcataacagcggtcattgactggagcgaggcgatgttcggggattcccaatacgaggtcgccaacatcttcttctggaggccgtggttggcttgtatggagcagcagacgcgctacttcgagcggaggcatccggagcttgcaggatcgccgcggctccgggcgtatatgctccgcattggtcttgaccaactctatcagagcttggttgacggcaatttcgatgatgcagcttgggcgcagggtcgatgcgacgcaatcgtccgatccggagccgggactgtcgggcgtacacaaatcgcccgcagaagcgcggccgtctggaccgatggctgtgtagaagtactcgccgatagtggaaaccgacgccccagcactcgtccgagggcaaaggaatagagtagatgccgaccgggatcgatcccccgatcctaacaccgggttgtgtggccaaaattgttctgtagtcgctgtgagttgacacggctagtgcttatgattttcctcgcgtgtggtgcctgtactcagccctatgccttatttgcaacacatttacgtacagcgcacaagagaagagaagatcacttgaagataataaatatagggttgtaggcatcttgtttaactcaaattttctcgtcttggtgtgtcgacatgattgaaatagtgccaccagttgtgtttgatgcgtttgttatctatgcagtattctgcaaggccttgcaaggccttgcaggcatgcaagctagcttgtattctatagtgtcacctaaatcgtatgtgtatgatacataaggttatgtattaattgtagccgcgttctaacgacaatatgtacaagcctaattgtgtagcatctggcttactgaagcagaccctatcatctctctcgtaaactgccgtcagagtcggtttggttggacgaaccttctgagtttctggtaacgccgttccgcaccccggaaatggtcagcgaaccaatcagcagggtcatcgctagccagatcctctacgccggacgcatcgtggccggcatcaccggcgccacaggtgcggttgctggcgcctatatcgccgacatcaccgatggggaagatcgggctcgccacttcgggctcatgagcgcttgtttcggcgtgggtatggtggcaggccccgtggccgggggactgttgggcgccatctccttgcaccattccttgcggcggcggtgctcaacggcctcaacctactactgggctgcttcctaatgcaggagtcgcataagggagagcgtcgatatggtgcactctcagtacaatctgctctgatgccgcatagttaagccagccccgacacccgccaacacccgctgacgcgccctgacgggcttgtctgctcccggcatccgcttacagacaagctgtgaccgtctccgggagctgcatgtgtcagaggttttcaccgtcatcaccgaaacgcgcgagacgaaagggcctcgtgatacgcctatttttataggttaatgtcatgataataatggtttcttagacgtcaggtggcacttttcggggaaatgtgcgcggaacccctatttgtttatttttctaaatacattcaaatatgtatccgctcatgagacaataaccctgataaatgcttcaataatattgaaaaaggaagagtatgagtattcaacatttccgtgtcgcccttattcccttttttgcggcattttgccttcctgtttttgctcacccagaaacgctggtgaaagtaaaagatgctgaagatcagttgggtgcacgagtgggttacatcgaactggatctcaacagcggtaagatccttgagagttttcgccccgaagaacgttttccaatgatgagcacttttaaagttctgctatgtggcgcggtattatcccgtattgacgccgggcaagagcaactcggtcgccgcatacactattctcagaatgacttggttgagtactcaccagtcacagaaaagcatcttacggatggcatgacagtaagagaattatgcagtgctgccataaccatgagtgataacactgcggccaacttacttctgacaacgatcggaggaccgaaggagctaaccgcttttttgcacaacatgggggatcatgtaactcgccttgatcgttgggaaccggagctgaatgaagccataccaaacgacgagcgtgacaccacgatgcctgtagcaatggcaacaacgttgcgcaaactattaactggcgaactacttactctagcttcccggcaacaattaatagactggatggaggcggataaagttgcaggaccacttctgcgctcggcccttccggctggctggtttattgctgataaatctggagccggtgagcgtgggtctcgcggtatcattgcagcactggggccagatggtaagccctcccgtatcgtagttatctacacgacggggagtcaggcaactatggatgaacgaaatagacagatcgctgagataggtgcctcactgattaagcattggtaactgtcagaccaagtttactcatatatactttagattgatttaaaacttcatttttaatttaaaaggatctaggtgaagatcctttttgataatctcatgaccaaaatcccttaacgtgagttttcgttccactgagcgtcagaccccgtagaaaagatcaaaggatcttcttgagatcctttttttctgcgcgtaatctgctgcttgcaaacaaaaaaaccaccgctaccagcggtggtttgtttgccggatcaagagctaccaactctttttccgaaggtaactggcttcagcagagcgcagataccaaatactgtccttctagtgtagccgtagttaggccaccacttcaagaactctgtagcaccgcctacatacctcgctctgctaatcctgttaccagtggctgctgccagtggcgataagtcgtgtcttaccgggttggactcaagacgatagttaccggataaggcgcagcggtcgggctgaacggggggttcgtgcacacagcccagcttggagcgaacgacctacaccgaactgagatacctacagcgtgagctatgagaaagcgccacgcttcccgaagggagaaaggcggacaggtatccggtaagcggcagggtcggaacaggagagcgcacgagggagcttccagggggaaacgcctggtatctttatagtcctgtcgggtttcgccacctctgacttgagcgtcgatttttgtgatgctcgtcaggggggcggagcctatggaaaaacgccagcaacgcggcctttttacggttcctggccttttgctggccttttgctcacatgttctttcctgcgttatcccctgattctgtggataaccgtattaccgcctttgagtgagctgataccgctcgccgcagccgaacgaccgagcgcagcgagtcagtgagcgaggaagcggaagagcgcccaatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcttatcgaaattaatacgactcactatagggagaccgtcggtaccgtcatt
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