EPR Plasmid #196: pHD1701
Plasmid Details
| Purpose | Inducible (pol I) expression of N-terminally 2x-myc-tagged proteins in Trypanosoma brucei. ACT 3'-UTR, hyg resistance. ATG:2xMyc:Hin-Apa-Hpa-BamMCS:Stop. Can add extra stop before MCS if wish. |
| Depositing Lab |
Christine Clayton Heidelberg University |
| Depositing Scientist | Christine Clayton |
| Antibiotic Resistance | Ampicillin |
| Bacterial Growth Temp | 37 |
| Copy Number | High |
| Selectable Marker | Hygromycin |
| Reference |
Depletion of GIM5 causes cellular fragility, a decreased glycosome number, and reduced levels of ether-linked phospholipids in trypanosomes. Voncken F et al. J Biol Chem 2003 PubMed See other plasmids from this publication |
| MTA and Terms |
MTA (PDF download) |
How to Cite This Reagent
IN THE MATERIALS & METHODS:
pHD1701 was a gift from Christine Clayton (European Plasmid Repository plasmid #196).
pHD1701 was a gift from Christine Clayton (European Plasmid Repository plasmid #196).
IN THE REFERENCES:
Voncken F et al.. Depletion of GIM5 causes cellular fragility, a decreased glycosome number, and reduced levels of ether-linked phospholipids in trypanosomes.. J Biol Chem 2003 PubMed 12829709
Voncken F et al.. Depletion of GIM5 causes cellular fragility, a decreased glycosome number, and reduced levels of ether-linked phospholipids in trypanosomes.. J Biol Chem 2003 PubMed 12829709
Sequences
Provided by depositor
download sequence
GAATTCGAGCTCATATAGTTGGTATGTATTCTAATTCCAGACTACTGGCG TGGGATAAACATGTCCCCCTGATTAAAAGGAAAGATTCCATAGCCCATTA GTGCAAAGATAATTGGTACACCGTCAAAAACACATGGGAGACCACACGAT ACGACACTGTGACCGTAGCATCAATTCCGCACTCGATATAACTCTATCGA AGAACTTCCATGGTACAAACTGGTCGCCACGGTCTGCTCCACACGGAGAT CATCGTATCATTTTTATCGATAGCGGCCGCTATCGATGTATGCCTTGGCC CTGATGGCATGCCAATTTCACTACAACGGACTATGTGGACCCCGTTATCA TGGAAATGCGCTAGTTGGAGGAAGTTAGACCGCGCCGGAAAAGAGAGGGG TAGAGAAAATGACAACTTGGAAGATATCCACACACGCACGGTGAAACGTT AGCAACAATTATTAGGGAAGCACGCTTGCCCTAGTCCCACGAGTAACCAA GACTCCAAAAGCCTTTCTGGCACAGAGAGCGAGCCGAAATGGAAAAGAGA AACAATGCCTGCACTAACACTACTGAGCGATTCGCCTCGCCGCGGAGGAC CGAATACTAATAACGACACTTGCGGTCAAAAAGTAGAAGAACAAATGCTC AACGATGAGTGAATCAGGTTAGGGTAGTTGGAAAATTATACAGAATGTCT TTGGCAACACACCGGTACCGTCATTGGGGTTAAGCGGAAAGGTGTGTGTC AGTAGGTTGTGAGGTGAAAGCGTTTTCAGATGCATAGTGAGCTTAATGTC CTTTTCACAGTATATCGTGTCTGATAGGTATCTCTTATTAGTATAGTCGA ATACTAGTCAATAGTGCGTTTTGTGCAAAATGTCCATTTTGTGGCAGTGA TGGGGTTGTTTTATGCTATTCCGTGTCTCTGGGTGGGCGTGCATTGAAAA TAGGGGTTATCGGGTagggatctccctatcagtgatagagatctccctat cagtgatagagatccctGAGTACTGAGTTTAACATGTTCTCGTCCCGGGC TGCACGCGCCTTCGAGTTTTTTTTCCTTTTCCCCATTTTTTTCAACTTGA AGACTTCAATTACACCAAAAAGTAAAATTCACATGGGCGAGCAAAAGCTC ATTTCTGAAGAGGACTTGGGCGGAGGCTTAATTAACGGCGAGCAAAAGCT CATTTCTGAAGAGGACTTGAAGCTTcgcggggcccacgcgttctagagtt aacGGATCCTAACACCGGGTTGTGTGGCCAAAATTGTTCTGTAGTCGCTG TGAGTTGACACGGCTAGTGCTTATGATTTTCCTCGCGTGTGGTGCCTGTA CTCAGCCCTATGCCTTATTTGCAACACATTTACGTACAGCGCACAAGAGA AGAGAAGATCACTTGAAGATAATAAATATAGGGTTGTAGGCATCTTGTTT AACTCAAATTTTCTCGTCTTGGTGTGTCGACATGATTGAAATAGTGCCAC CAGTTGTGTTTGATGCGTTTGTTATCTATGCAGTATTCTGCAGTTTAATT TGTTGGATGAGCTATTTCATTAATTTTTTTGCCTTCTCTTCTTTTGGGTT CGCTCGAGTTTCCACCCAGCGCGGGTGCATTCTGGCTCTTATATATACTT ATTGTCATGACAGAGTATATTGTACTGTGTTGATAAGGGACGGGTAACTG TATTGAAGAGCCGATGCTTTTGACATGTTAGATATAATATGTTTTATTGT AAAGTCAATACAACACACAATAGGATAATAATGATAAAGTTAAAAAAGTA TATATAGTAATAGAAATATATCTTATATAGGAAAGATTAAGCAGTAAAAG TAGCGCTTACGGCGTACGGAGCAGGAGAGCAACTGACCGCTCTCAGAgcc cGGGCACAGCAAGGTCTTCTGAAATTCATGTTTTTTTTTTTTTTACTCTG CATTGCAGTCTCCGCTCTTATTTAGTTTTGCTTTACGTAAGGTCTCGTTG CTGCCATAAAATAAGCTctagaACTAGTGATGAAAAAGCCTGAACTCACC GCGACGTCTGTCGAGAAGTTTCTGATCGAAAAGTTCGACAGCGTCTCCGA CCTGATGCAGCTCTCGGAGGGCGAAGAATCTCGTGCTTTCAGCTTCGATG TAGGAGGGCGTGGATATGTCCTGCGGGTAAATAGCTGCGCCGATGGTTTC TACAAAGATCGTTATGTTTATCGGCACTTTGCATCGGCCGCGCTCCCGAT TCCGGAAGTGCTTGACATTGGGGAATTCAGCGAGAGCCTGACCTATTGCA TCTCCCGCCGTGCACAGGGTGTCACGTTGCAAGACCTGCCTGAAACCGAA CTGCCCGCTGTTCTGCAGCCGGTCGCGGAGGCCATGGATGCGATCGCTGC GGCCGATCTTAGCCAGACGAGCGGGTTCGGCCCATTCGGACCGCAAGGAA TCGGTCAATACACTACATGGCGTGATTTCATATGCGCGATTGCTGATCCC CATGTGTATCACTGGCAAACTGTGATGGACGACACCGTCAGTGCGTCCGT CGCGCAGGCTCTCGATGAGCTGATGCTTTGGGCCGAGGACTGCCCCGAAG TCCGGCACCTCGTGCACGCGGATTTCGGCTCCAACAATGTCCTGACGGAC AATGGCCGCATAACAGCGGTCATTGACTGGAGCGAGGCGATGTTCGGGGA TTCCCAATACGAGGTCGCCAACATCTTCTTCTGGAGGCCGTGGTTGGCTT GTATGGAGCAGCAGACGCGCTACTTCGAGCGGAGGCATCCGGAGCTTGCA GGATCGCCGCGGCTCCGGGCGTATATGCTCCGCATTGGTCTTGACCAACT CTATCAGAGCTTGGTTGACGGCAATTTCGATGATGCAGCTTGGGCGCAGG GTCGATGCGACGCAATCGTCCGATCCGGAGCCGGGACTGTCGGGCGTACA CAAATCGCCCGCAGAAGCGCGGCCGTCTGGACCGATGGCTGTGTAGAAGT ACTCGCCGATAGTGGAAACCGACGCCCCAGCACTCGTCCGAGGGCAAAGG AATAGAGTAGATGCCGACCGGGATCGATCCCCCGATCCTAACACCGGGTT GTGTGGCCAAAATTGTTCTGTAGTCGCTGTGAGTTGACACGGCTAGTGCT TATGATTTTCCTCGCGTGTGGTGCCTGTACTCAGCCCTATGCCTTATTTG CAACACATTTACGTACAGCGCACAAGAGAAGAGAAGATCACTTGAAGATA ATAAATATAGGGTTGTAGGCATCTTGTTTAACTCAAATTTTCTCGTCTTG GTGTGTCGACATGATTGAAATAGTGCCACCAGTTGTGTTTGATGCGTTTG TTATCTATGCAGTATTCTGCAAGGCCTTGCAAGGCCTTGCAGGCATGCAA GCTAGCTTGTATTCTATAGTGTCACCTAAATCGTATGTGTATGATACATA AGGTTATGTATTAATTGTAGCCGCGTTCTAACGACAATATGTACAAGCCT AATTGTGTAGCATCTGGCTTACTGAAGCAGACCCTATCATCTCTCTCGTA AACTGCCGTCAGAGTCGGTTTGGTTGGACGAACCTTCTGAGTTTCTGGTA ACGCCGTTCCGCACCCCGGAAATGGTCAGCGAACCAATCAGCAGGGTCAT CGCTAGCCAGATCCTCTACGCCGGACGCATCGTGGCCGGCATCACCGGCG CCACAGGTGCGGTTGCTGGCGCCTATATCGCCGACATCACCGATGGGGAA GATCGGGCTCGCCACTTCGGGCTCATGAGCGCTTGTTTCGGCGTGGGTAT GGTGGCAGGCCCCGTGGCCGGGGGACTGTTGGGCGCCATCTCCTTGCACC ATTCCTTGCGGCGGCGGTGCTCAACGGCCTCAACCTACTACTGGGCTGCT TCCTAATGCAGGAGTCGCATAAGGGAGAGCGTCGATATGGTGCACTCTCA GTACAATCTGCTCTGATGCCGCATAGTTAAGCCAGCCCCGACACCCGCCA ACACCCGCTGACGCGCCCTGACGGGCTTGTCTGCTCCCGGCATCCGCTTA CAGACAAGCTGTGACCGTCTCCGGGAGCTGCATGTGTCAGAGGTTTTCAC CGTCATCACCGAAACGCGCGAGACGAAAGGGCCTCGTGATACGCCTATTT TTATAGGTTAATGTCATGATAATAATGGTTTCTTAGACGTCAGGTGGCAC TTTTCGGGGAAATGTGCGCGGAACCCCTATTTGTTTATTTTTCTAAATAC ATTCAAATATGTATCCGCTCATGAGACAATAACCCTGATAAATGCTTCAA TAATATTGAAAAAGGAAGAGTATGAGTATTCAACATTTCCGTGTCGCCCT TATTCCCTTTTTTGCGGCATTTTGCCTTCCTGTTTTTGCTCACCCAGAAA CGCTGGTGAAAGTAAAAGATGCTGAAGATCAGTTGGGTGCACGAGTGGGT TACATCGAACTGGATCTCAACAGCGGTAAGATCCTTGAGAGTTTTCGCCC CGAAGAACGTTTTCCAATGATGAGCACTTTTAAAGTTCTGCTATGTGGCG CGGTATTATCCCGTATTGACGCCGGGCAAGAGCAACTCGGTCGCCGCATA CACTATTCTCAGAATGACTTGGTTGAGTACTCACCAGTCACAGAAAAGCA TCTTACGGATGGCATGACAGTAAGAGAATTATGCAGTGCTGCCATAACCA TGAGTGATAACACTGCGGCCAACTTACTTCTGACAACGATCGGAGGACCG AAGGAGCTAACCGCTTTTTTGCACAACATGGGGGATCATGTAACTCGCCT TGATCGTTGGGAACCGGAGCTGAATGAAGCCATACCAAACGACGAGCGTG ACACCACGATGCCTGTAGCAATGGCAACAACGTTGCGCAAACTATTAACT GGCGAACTACTTACTCTAGCTTCCCGGCAACAATTAATAGACTGGATGGA GGCGGATAAAGTTGCAGGACCACTTCTGCGCTCGGCCCTTCCGGCTGGCT GGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATC ATTGCAGCACTGGGGCCAGATGGTAAGCCCTCCCGTATCGTAGTTATCTA CACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTG AGATAGGTGCCTCACTGATTAAGCATTGGTAACTGTCAGACCAAGTTTAC TCATATATACTTTAGATTGATTTAAAACTTCATTTTTAATTTAAAAGGAT CTAGGTGAAGATCCTTTTTGATAATCTCATGACCAAAATCCCTTAACGTG AGTTTTCGTTCCACTGAGCGTCAGACCCCGTAGAAAAGATCAAAGGATCT TCTTGAGATCCTTTTTTTCTGCGCGTAATCTGCTGCTTGCAAACAAAAAA ACCACCGCTACCAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTC TTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTC CTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACC GCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTG GCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGAT AAGGCGCAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGCCCAGCTT GGAGCGAACGACCTACACCGAACTGAGATACCTACAGCGTGAGCTATGAG AAAGCGCCACGCTTCCCGAAGGGAGAAAGGCGGACAGGTATCCGGTAAGC GGCAGGGTCGGAACAGGAGAGCGCACGAGGGAGCTTCCAGGGGGAAACGC CTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTC GATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCAGC AACGCGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTCACAT GTTCTTTCCTGCGTTATCCCCTGATTCTGTGGATAACCGTATTACCGCCT TTGAGTGAGCTGATACCGCTCGCCGCAGCCGAACGACCGAGCGCAGCGAG TCAGTGAGCGAGGAAGCGGAAGAGCGCCCAATACGCAAACCGCCTCTCCC CGCGCGTTGGCCGATTCATTAATGCAGCTGGCTTATCGAAATTAATACGA CTCACTATAGGGAGACCG
GAATTCGAGCTCATATAGTTGGTATGTATTCTAATTCCAGACTACTGGCG TGGGATAAACATGTCCCCCTGATTAAAAGGAAAGATTCCATAGCCCATTA GTGCAAAGATAATTGGTACACCGTCAAAAACACATGGGAGACCACACGAT ACGACACTGTGACCGTAGCATCAATTCCGCACTCGATATAACTCTATCGA AGAACTTCCATGGTACAAACTGGTCGCCACGGTCTGCTCCACACGGAGAT CATCGTATCATTTTTATCGATAGCGGCCGCTATCGATGTATGCCTTGGCC CTGATGGCATGCCAATTTCACTACAACGGACTATGTGGACCCCGTTATCA TGGAAATGCGCTAGTTGGAGGAAGTTAGACCGCGCCGGAAAAGAGAGGGG TAGAGAAAATGACAACTTGGAAGATATCCACACACGCACGGTGAAACGTT AGCAACAATTATTAGGGAAGCACGCTTGCCCTAGTCCCACGAGTAACCAA GACTCCAAAAGCCTTTCTGGCACAGAGAGCGAGCCGAAATGGAAAAGAGA AACAATGCCTGCACTAACACTACTGAGCGATTCGCCTCGCCGCGGAGGAC CGAATACTAATAACGACACTTGCGGTCAAAAAGTAGAAGAACAAATGCTC AACGATGAGTGAATCAGGTTAGGGTAGTTGGAAAATTATACAGAATGTCT TTGGCAACACACCGGTACCGTCATTGGGGTTAAGCGGAAAGGTGTGTGTC AGTAGGTTGTGAGGTGAAAGCGTTTTCAGATGCATAGTGAGCTTAATGTC CTTTTCACAGTATATCGTGTCTGATAGGTATCTCTTATTAGTATAGTCGA ATACTAGTCAATAGTGCGTTTTGTGCAAAATGTCCATTTTGTGGCAGTGA TGGGGTTGTTTTATGCTATTCCGTGTCTCTGGGTGGGCGTGCATTGAAAA TAGGGGTTATCGGGTagggatctccctatcagtgatagagatctccctat cagtgatagagatccctGAGTACTGAGTTTAACATGTTCTCGTCCCGGGC TGCACGCGCCTTCGAGTTTTTTTTCCTTTTCCCCATTTTTTTCAACTTGA AGACTTCAATTACACCAAAAAGTAAAATTCACATGGGCGAGCAAAAGCTC ATTTCTGAAGAGGACTTGGGCGGAGGCTTAATTAACGGCGAGCAAAAGCT CATTTCTGAAGAGGACTTGAAGCTTcgcggggcccacgcgttctagagtt aacGGATCCTAACACCGGGTTGTGTGGCCAAAATTGTTCTGTAGTCGCTG TGAGTTGACACGGCTAGTGCTTATGATTTTCCTCGCGTGTGGTGCCTGTA CTCAGCCCTATGCCTTATTTGCAACACATTTACGTACAGCGCACAAGAGA AGAGAAGATCACTTGAAGATAATAAATATAGGGTTGTAGGCATCTTGTTT AACTCAAATTTTCTCGTCTTGGTGTGTCGACATGATTGAAATAGTGCCAC CAGTTGTGTTTGATGCGTTTGTTATCTATGCAGTATTCTGCAGTTTAATT TGTTGGATGAGCTATTTCATTAATTTTTTTGCCTTCTCTTCTTTTGGGTT CGCTCGAGTTTCCACCCAGCGCGGGTGCATTCTGGCTCTTATATATACTT ATTGTCATGACAGAGTATATTGTACTGTGTTGATAAGGGACGGGTAACTG TATTGAAGAGCCGATGCTTTTGACATGTTAGATATAATATGTTTTATTGT AAAGTCAATACAACACACAATAGGATAATAATGATAAAGTTAAAAAAGTA TATATAGTAATAGAAATATATCTTATATAGGAAAGATTAAGCAGTAAAAG TAGCGCTTACGGCGTACGGAGCAGGAGAGCAACTGACCGCTCTCAGAgcc cGGGCACAGCAAGGTCTTCTGAAATTCATGTTTTTTTTTTTTTTACTCTG CATTGCAGTCTCCGCTCTTATTTAGTTTTGCTTTACGTAAGGTCTCGTTG CTGCCATAAAATAAGCTctagaACTAGTGATGAAAAAGCCTGAACTCACC GCGACGTCTGTCGAGAAGTTTCTGATCGAAAAGTTCGACAGCGTCTCCGA CCTGATGCAGCTCTCGGAGGGCGAAGAATCTCGTGCTTTCAGCTTCGATG TAGGAGGGCGTGGATATGTCCTGCGGGTAAATAGCTGCGCCGATGGTTTC TACAAAGATCGTTATGTTTATCGGCACTTTGCATCGGCCGCGCTCCCGAT TCCGGAAGTGCTTGACATTGGGGAATTCAGCGAGAGCCTGACCTATTGCA TCTCCCGCCGTGCACAGGGTGTCACGTTGCAAGACCTGCCTGAAACCGAA CTGCCCGCTGTTCTGCAGCCGGTCGCGGAGGCCATGGATGCGATCGCTGC GGCCGATCTTAGCCAGACGAGCGGGTTCGGCCCATTCGGACCGCAAGGAA TCGGTCAATACACTACATGGCGTGATTTCATATGCGCGATTGCTGATCCC CATGTGTATCACTGGCAAACTGTGATGGACGACACCGTCAGTGCGTCCGT CGCGCAGGCTCTCGATGAGCTGATGCTTTGGGCCGAGGACTGCCCCGAAG TCCGGCACCTCGTGCACGCGGATTTCGGCTCCAACAATGTCCTGACGGAC AATGGCCGCATAACAGCGGTCATTGACTGGAGCGAGGCGATGTTCGGGGA TTCCCAATACGAGGTCGCCAACATCTTCTTCTGGAGGCCGTGGTTGGCTT GTATGGAGCAGCAGACGCGCTACTTCGAGCGGAGGCATCCGGAGCTTGCA GGATCGCCGCGGCTCCGGGCGTATATGCTCCGCATTGGTCTTGACCAACT CTATCAGAGCTTGGTTGACGGCAATTTCGATGATGCAGCTTGGGCGCAGG GTCGATGCGACGCAATCGTCCGATCCGGAGCCGGGACTGTCGGGCGTACA CAAATCGCCCGCAGAAGCGCGGCCGTCTGGACCGATGGCTGTGTAGAAGT ACTCGCCGATAGTGGAAACCGACGCCCCAGCACTCGTCCGAGGGCAAAGG AATAGAGTAGATGCCGACCGGGATCGATCCCCCGATCCTAACACCGGGTT GTGTGGCCAAAATTGTTCTGTAGTCGCTGTGAGTTGACACGGCTAGTGCT TATGATTTTCCTCGCGTGTGGTGCCTGTACTCAGCCCTATGCCTTATTTG CAACACATTTACGTACAGCGCACAAGAGAAGAGAAGATCACTTGAAGATA ATAAATATAGGGTTGTAGGCATCTTGTTTAACTCAAATTTTCTCGTCTTG GTGTGTCGACATGATTGAAATAGTGCCACCAGTTGTGTTTGATGCGTTTG TTATCTATGCAGTATTCTGCAAGGCCTTGCAAGGCCTTGCAGGCATGCAA GCTAGCTTGTATTCTATAGTGTCACCTAAATCGTATGTGTATGATACATA AGGTTATGTATTAATTGTAGCCGCGTTCTAACGACAATATGTACAAGCCT AATTGTGTAGCATCTGGCTTACTGAAGCAGACCCTATCATCTCTCTCGTA AACTGCCGTCAGAGTCGGTTTGGTTGGACGAACCTTCTGAGTTTCTGGTA ACGCCGTTCCGCACCCCGGAAATGGTCAGCGAACCAATCAGCAGGGTCAT CGCTAGCCAGATCCTCTACGCCGGACGCATCGTGGCCGGCATCACCGGCG CCACAGGTGCGGTTGCTGGCGCCTATATCGCCGACATCACCGATGGGGAA GATCGGGCTCGCCACTTCGGGCTCATGAGCGCTTGTTTCGGCGTGGGTAT GGTGGCAGGCCCCGTGGCCGGGGGACTGTTGGGCGCCATCTCCTTGCACC ATTCCTTGCGGCGGCGGTGCTCAACGGCCTCAACCTACTACTGGGCTGCT TCCTAATGCAGGAGTCGCATAAGGGAGAGCGTCGATATGGTGCACTCTCA GTACAATCTGCTCTGATGCCGCATAGTTAAGCCAGCCCCGACACCCGCCA ACACCCGCTGACGCGCCCTGACGGGCTTGTCTGCTCCCGGCATCCGCTTA CAGACAAGCTGTGACCGTCTCCGGGAGCTGCATGTGTCAGAGGTTTTCAC CGTCATCACCGAAACGCGCGAGACGAAAGGGCCTCGTGATACGCCTATTT TTATAGGTTAATGTCATGATAATAATGGTTTCTTAGACGTCAGGTGGCAC TTTTCGGGGAAATGTGCGCGGAACCCCTATTTGTTTATTTTTCTAAATAC ATTCAAATATGTATCCGCTCATGAGACAATAACCCTGATAAATGCTTCAA TAATATTGAAAAAGGAAGAGTATGAGTATTCAACATTTCCGTGTCGCCCT TATTCCCTTTTTTGCGGCATTTTGCCTTCCTGTTTTTGCTCACCCAGAAA CGCTGGTGAAAGTAAAAGATGCTGAAGATCAGTTGGGTGCACGAGTGGGT TACATCGAACTGGATCTCAACAGCGGTAAGATCCTTGAGAGTTTTCGCCC CGAAGAACGTTTTCCAATGATGAGCACTTTTAAAGTTCTGCTATGTGGCG CGGTATTATCCCGTATTGACGCCGGGCAAGAGCAACTCGGTCGCCGCATA CACTATTCTCAGAATGACTTGGTTGAGTACTCACCAGTCACAGAAAAGCA TCTTACGGATGGCATGACAGTAAGAGAATTATGCAGTGCTGCCATAACCA TGAGTGATAACACTGCGGCCAACTTACTTCTGACAACGATCGGAGGACCG AAGGAGCTAACCGCTTTTTTGCACAACATGGGGGATCATGTAACTCGCCT TGATCGTTGGGAACCGGAGCTGAATGAAGCCATACCAAACGACGAGCGTG ACACCACGATGCCTGTAGCAATGGCAACAACGTTGCGCAAACTATTAACT GGCGAACTACTTACTCTAGCTTCCCGGCAACAATTAATAGACTGGATGGA GGCGGATAAAGTTGCAGGACCACTTCTGCGCTCGGCCCTTCCGGCTGGCT GGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATC ATTGCAGCACTGGGGCCAGATGGTAAGCCCTCCCGTATCGTAGTTATCTA CACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTG AGATAGGTGCCTCACTGATTAAGCATTGGTAACTGTCAGACCAAGTTTAC TCATATATACTTTAGATTGATTTAAAACTTCATTTTTAATTTAAAAGGAT CTAGGTGAAGATCCTTTTTGATAATCTCATGACCAAAATCCCTTAACGTG AGTTTTCGTTCCACTGAGCGTCAGACCCCGTAGAAAAGATCAAAGGATCT TCTTGAGATCCTTTTTTTCTGCGCGTAATCTGCTGCTTGCAAACAAAAAA ACCACCGCTACCAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTC TTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTC CTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACC GCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTG GCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGAT AAGGCGCAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGCCCAGCTT GGAGCGAACGACCTACACCGAACTGAGATACCTACAGCGTGAGCTATGAG AAAGCGCCACGCTTCCCGAAGGGAGAAAGGCGGACAGGTATCCGGTAAGC GGCAGGGTCGGAACAGGAGAGCGCACGAGGGAGCTTCCAGGGGGAAACGC CTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTC GATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCAGC AACGCGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTCACAT GTTCTTTCCTGCGTTATCCCCTGATTCTGTGGATAACCGTATTACCGCCT TTGAGTGAGCTGATACCGCTCGCCGCAGCCGAACGACCGAGCGCAGCGAG TCAGTGAGCGAGGAAGCGGAAGAGCGCCCAATACGCAAACCGCCTCTCCC CGCGCGTTGGCCGATTCATTAATGCAGCTGGCTTATCGAAATTAATACGA CTCACTATAGGGAGACCG
