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EPR Plasmid #2: pCasper4-hsp70 promoter-SV40 polyA
Purpose of plasmid To make transgenic Drosophila with heatshock inducible expression of an ORF of interest
Depositing Lab Aurelio Teleman
DKFZ
Depositing Scientist Aurelio Teleman
Antibiotic Resistance Ampicillin
Bacterial Growth Temp 37
Copy Number High
Selectable Marker Drosophila w+
Reference Insulin/IGF signaling drives cell proliferation in part via Yorkie/YAP.
Stra├čburger K et al. Dev Biol 2012
PubMed DOI
MTA and Terms MTA (PDF download)

How to cite this reagent:
In the Materials & Methods:
pCasper4-hsp70 promoter-SV40 polyA was a gift from Aurelio Teleman (European Plasmid Repository plasmid #2).

In the References:
Stra├čburger K et al.. Insulin/IGF signaling drives cell proliferation in part via Yorkie/YAP.. Dev Biol 2012 doi:10.1016/j.ydbio.2012.05.008 PubMed 22609549


Sequence: download sequence
gaattcGGTACCGAGCTCTTACGCGTGCTAGCCCGGGCTCGAGATCTGAGCGCCGGAGTATAAATAGAGGCGCTTCGTCTACGGAGCGACAATTCAATTCAAACAAGCAAAGTGAACACGTCGCTAAGCGAAAGCTAAGCAAATAAACAAGCGCAGCTGAACAAGCTAAACAATCTGCAGAAGCTggccgcataggccactagtggatctggatcctctagagtcgacctcgaacgttaacgttaacgtaacgttaaCTCGAGCTAGATCATAATCAGCCATACCACATTTGTAAAGGTTTTACCGCGAGAAAAAACCTCCCACACCTCCCCCTGAACCTGAAACATAACTTGTCTGCGTTGTTGTTGTTAACTTGTTTATTGCAGCTTATAATGGTTACAAATAAAGCAATAGCATCACAAATTTCACAAATAAAGCATTTTTTTCACTGCATTCTAGTTGTGGTTTGTCCAAACTCATCAATGTATgtcgaggtcgacctgcagcccaagctttgcgtactcgcaaattattaaaaataaaactttaaaaataatttcgtctaattaatattatgagttaattcaaaccccacggacatgctaagggttaatcaacaatcatatcgctgtctcactcagactcaatacgacactcagaatactattcctttcactcgcacttattgcaagcatacgttaagtggatgtctcttgccgacgggaccaccttatgttatttcatcatggtctggccattctcatcgtgagcttccgggtgctcgcatatctggctctaagacttcgggcccgacgcaaggagtagccgacatatatccgaaataactgcttgtttttttttttaccattattaccatcgtgtttactgtttattgccccctcaaaaagctaatgtaattatatttgtgccaataaaaacaagatatgacctatagaatacaagtatttccccttcgaacatccccacaagtagactttggatttgtcttctaaccaaaagacttacacacctgcataccttacatcaaaaactcgtttatcgctacataaaacaccgggatatattttttatatacatacttttcaaatcgcgcgccctcttcataattcacctccaccacaccacgtttcgtagttgctctttcgctgtctcccacccgctctccgcaacacattcaccttttgttcgacgaccttggagcgactgtcgttagttccgcgcgattcggtgcggtatttcacaccgcatatggtgcactctcagtacaatctgctctgatgccgcatagttaagccagccccgacacccgccaacacccgctgacgcgccctgacgggcttgtctgctcccggcatccgcttacagacaagctgtgaccgtctccgggagctgcatgtgtcagaggttttcaccgtcatcaccgaaacgcgcgagacgaaagggcctcgtgatacgcctatttttataggttaatgtcatgataataatggtttcttagacgtcaggtggcacttttcggggaaatgtgcgcggaacccctatttgtttatttttctaaatacattcaaatatgtatccgctcatgagacaataaccctgataaatgcttcaataatattgaaaaaggaagagtatgagtattcaacatttccgtgtcgcccttattcccttttttgcggcattttgccttcctgtttttgctcacccagaaacgctggtgaaagtaaaagatgctgaagatcagttgggtgcacgagtgggttacatcgaactggatctcaacagcggtaagatccttgagagttttcgccccgaagaacgttttccaatgatgagcacttttaaagttctgctatgtggcgcggtattatcccgtattgacgccgggcaagagcaactcggtcgccgcatacactattctcagaatgacttggttgagtactcaccagtcacagaaaagcatcttacggatggcatgacagtaagagaattatgcagtgctgccataaccatgagtgataacactgcggccaacttacttctgacaacgatcggaggaccgaaggagctaaccgcttttttgcacaacatgggggatcatgtaactcgccttgatcgttgggaaccggagctgaatgaagccataccaaacgacgagcgtgacaccacgatgcctgtagcaatggcaacaacgttgcgcaaactattaactggcgaactacttactctagcttcccggcaacaattaatagactggatggaggcggataaagttgcaggaccacttctgcgctcggcccttccggctggctggtttattgctgataaatctggagccggtgagcgtgggtctcgcggtatcattgcagcactggggccagatggtaagccctcccgtatcgtagttatctacacgacggggagtcaggcaactatggatgaacgaaatagacagatcgctgagataggtgcctcactgattaagcattggtaactgtcagaccaagtttactcatatatactttagattgatttaaaacttcatttttaatttaaaaggatctaggtgaagatcctttttgataatctcatgaccaaaatcccttaacgtgagttttcgttccactgagcgtcagaccccgtagaaaagatcaaaggatcttcttgagatcctttttttctgcgcgtaatctgctgcttgcaaacaaaaaaaccaccgctaccagcggtggtttgtttgccggatcaagagctaccaactctttttccgaaggtaactggcttcagcagagcgcagataccaaatactgtccttctagtgtagccgtagttaggccaccacttcaagaactctgtagcaccgcctacatacctcgctctgctaatcctgttaccagtggctgctgccagtggcgataagtcgtgtcttaccgggttggactcaagacgatagttaccggataaggcgcagcggtcgggctgaacggggggttcgtgcacacagcccagcttggagcgaacgacctacaccgaactgagatacctacagcgtgagcattgagaaagcgccacgcttcccgaagggagaaaggcggacaggtatccggtaagcggcagggtcggaacaggagagcgcacgagggagcttccagggggaaacgcctggtatctttatagtcctgtcgggtttcgccacctctgacttgagcgtcgatttttgtgatgctcgtcaggggggcggagcctatggaaaaacgccttcttcttgaactcgggctcggtgccagtatacctcaaatggttgtcgtacctctcatggttccgttacgccaacgagggtctgctgattaaccaatgggcggacgtggagccgggcgaaattagctgcacatcgtcgaacaccacgtgccccagttcgggcaaggtcatcctggagacgcttaacttctccgccgccgatctgccgctggactacgtgggtctggcccatgatgaaataacataaggtggtcccgtcgatagccgaagcttaccgaagtatacacttaaattcagtgcacgtttgcttgttgagaggaaaggttgtgtgcggacgaatttttttttgaaaacattaacccttacgtggaataaaaaaaaatgaaatattgcaaattttgctgcaaagctgtgactggagtaaaattaattcacgtgccgaagtgtgctattaagagaaaattgtgggagcagagccttgggtgcagccttggtgaaaactcccaaatttgtgatacccactttaaagattcgcagtggaaggctgcacctgcaaaaggtcagacatttaaaaggaggcgactcaacgcagatgccgtacctagtaaagtgatagagcctgaaccagaaaagataaaagaaggctataccagtgggagtacacaaacagagtaagtttgaatagtaaaaaaaatcatttatgtaaacaataacgtgactgtgcgttaggtcctgttcattgtttaatgaaaataagagcttgagggaaaaaattcgtactttggagtacgaaatgcgtcgtttagagcagcagccgaattaattctagttccagtgaaatccaagcattttctaaattaaatgtattcttattattatagttgttatttttgatatatataaacaacactattatgcccaccatttttttgagatgcatctacacaaggaacaaacactggatgtcactttcagttcaaattgtaacgctaatcactccgaacaggtcacaaaaaattaccttaaaaagtcataatattaaattagaataaatatagctgtgagggaaatatatacaaatatattggagcaaataaattgtacatacaaatatttattactaatttctattgagacgaaatgaaccactcggaaccatttgagcgaaccgaatcgcgcggaactaacgacagtcgctccaaggtcgtcgaacaaaaggtgaatgtgttgcggagagcgggtgggagacagcgaaagagcaactacgaaacgtggtgtggtggaggtgaattatgaagagggcgcgcgatttgaaaagtatgtatataaaaaatatatcccggtgttttatgtagcgataaacgagtttttgatgtaaggtatgcaggtgtgtaagtcttttggttagaagacaaatccaaagtctacttgtggggatgttcgaaggggaaatacttgtattctataggtcatatcttgtttttattggcacaaatataattacattagctttttgagggggcaataaacagtaaacacgatggtaataatggtaaaaaaaaaaacaagcagttatttcggatatatgtcggctactccttgcgtcgggcccgaagtcttagagccagatatgcgagcacccggaagctcacgatgagaatggccagacccacgtagtccagcggcagatcggcggcggagaagttaagcgtctccaggatgaccttgcccgaactggggcacgtggtgttcgacgatgtgcagctaatttcgcccggctccacgtccgcccattggttaatcagcagaccctcgttggcgtaacggaaccatgagaggtacgacaaccatttgaggtatactggcaccgagcccgagttcaagaagaagccgccaaagagcaggaatggtatgataaccggcggacccacagacagcgccatcgaggtcgaggagctggcgcaggatattagatatccgaaggacgttgacacattggccaccagagtgaccagcgccaggcagttgaagaagtgcagcactccggcccgcagtccgatcatcggataggcaatcgccgtgaagaccagtggcactgtgagaaaaagcggtaattcggcaatcgttttgcccagaaagtatgtgtcacagcgataaagtcgacttcgggcctccctcataaaaactggcagctctgaggtgaacacctaaatcgaatcgattcattagaaagttagtaaattattaatatgcaaatgtattctaaacaagacttacatttatcgtggcaaagacgttttgaaaggtcatgttggtcaggaagaggaagatggctccgttgatattcatcacgcccacttgcgtgagttgttggcccaaaaagatgaggccaatcaagatggcaaccatctgcaaattaaaatgttactcgcatctcattaatattcatatcttcaacatgttcgcgagttaaatgaaatttatttattttctgcaaaactataaactatacatctcattgaaaaaaactaagaagggtgtggaatcaggcaattctaactaaaatctagcgaatttgtttccaagaattgtaagcgttatatcatttgtttccactggaaccactcaccgttgtctgaataagtcgcacttttacgaggagtggttccttgagcaccgacagccaggatcgccacaggaccgcccggaactgcatgaaccaggtggccttgtaggtgtacccattctccggctgctccagtggcttctccaaatttttggtggccaacaactgctccatatcccgggctactttgctaatagcaaaattgtcgccatatcttggcgatccgatcacgggactcgatctcccgtccgggcacaacggccaacacctgtacgtaaaagtccgccggattgtagttggtaggacactgggcacccacgctggataggagttgagatgtaatgtaatgctagatacccttaataaacacatcgaactcactaggaaaagaagtcgacggcttcgctgggagtgcccaagaaagctaccctgccctcggccatcagaaggatcttgtcaaagagctcaaacagctcggaagacggctgatgaatggtcaggatgacggtcttgcccttctgcgacagcttcttcagcacctggacgacgctgtgggcggtaaatgagtccagtccggaggtgggctcatcgcagatcagaagcggcggatcggttagtgcctcggaggcgaatgccagacgcttcctttctccgccggacagacctttcaccctgccgggcacaccgatgatcgtgtgctgacatttgctgagcgaaagctcctggatcacctgatccacgcgggccactcgctgccgataggtcagatgtcgtggcatccgcaccatggcctggaaaatcaggtgttccctggccgttagggagccgataaagaggtcatcctgctggacataggcgcacctggcctgcatctccttggcgtccacaggttggccattgagcagtcgcatcccggatggcgatacttggatgccctgcggcgatcgaaaggcaagggcattcagcagggtcgtctttccggcaccggaactgcccatcacggccaaaagttcgcccggataggccacgccgcaaactgagtttcaaattggtaattggaccctttattaagatttcacacagatcagccgactgcgaatagaaactcaccgttcttgagcaaatgtttcctgggcgccggtatgtgtcgctcgttgcagaatagtccgcgtgtccggttgaccagctgccgccatccggagcccggctgattgaccgccccaaagatgtccatattgtgccaggcataggtgaggttctcggctagttggccgctccctgaaccggagtcctccggcggactgggtggcaggagcgtgccgtagtttttggcctgcccgaagccctggttaatgcagctctgcgaagcgtccgctgtcaccctgcaatgataggggatctcaaatatcaactacaagcgttatgctcatctaaccccgaacaaaacgaagtatcctacgaagtaggtttatacttttatttattttttgtgcatctaggatcagcttaaaatatctggttgttatattttttgtaaaaaagaatgtagtcgaaaatgaatgcctttagatgtcttgatcatgatatgatcttaaaaattgtcttatatagcgagcacagctaccagaataatctgtttcgtgtcactatttgtttgtgcgattgcggtttgggatttttgtgggtcgcagttctcacgccgcagacaatttgatgttgcaatcgcagttcctatagatcaagtgaacttaagatgtatgcacatgtactactcacattgttcagatgctcggcagatgggtgtttgctgcctccgcgaattaatagctcctgatcctcttggcccattgccgggatttttcacactttcccctgcttacccacccaaaaccaatcaccaccccaatcactcaaaaaacaaacaaaaataagaagcgagaggagttttggcacagcactttgtgtttaattgatggcgtaaaccgcttggagcttcgtcacgaaaccgctgacaaagtgcaactgaaggcggacattgacgctaggtaacgctacaaacggtggcgaaagagatagcggacgcagcggcgaaagagacggcgatatttctgtggacagagaaggaggcaaacagcgctgactttgagtggaatgtcattttgagtgagaggtaatcgaaagaacctggtacttcaaatacccttggatcgaagtaaatttaaaactgatcagataagttcaatgatatccagtgcagtaaaaaataaaaaaaaaatatgtttttttaaatctacattctccaaaaaagggttttattaacttacatacatacta



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