EPR Plasmid #217: pHD2295
Sequence: download sequence
| Purpose of plasmid | Inducible expression of lambdaN-PABP1-2myc in Trypanosoma brucei. Positive control for tethering experiments with reporters with boxB in the 3'-UTR. Sequence was pasted together in 2022 and may not be completely correct. |
| Depositing Lab |
Christine Clayton Heidelberg University |
| Depositing Scientist | Christine Clayton |
| Antibiotic Resistance | Ampicillin |
| Bacterial Growth Temp | 37 |
| Copy Number | High |
| Selectable Marker | Hygromycin |
| Reference |
A genome-wide tethering screen reveals novel potential post-transcriptional regulators in Trypanosoma brucei. Erben ED et al. PLoS Pathog 2014 PubMed DOI |
| MTA and Terms |
MTA (PDF download) |
How to cite this reagent:
In the Materials & Methods:
pHD2295 was a gift from Christine Clayton (European Plasmid Repository plasmid #217).
In the References:
Erben ED et al.. A genome-wide tethering screen reveals novel potential post-transcriptional regulators in Trypanosoma brucei.. PLoS Pathog 2014 doi:10.1371/journal.ppat.1004178 PubMed 24945722
pHD2295 was a gift from Christine Clayton (European Plasmid Repository plasmid #217).
In the References:
Erben ED et al.. A genome-wide tethering screen reveals novel potential post-transcriptional regulators in Trypanosoma brucei.. PLoS Pathog 2014 doi:10.1371/journal.ppat.1004178 PubMed 24945722
Sequence: download sequence
GAATTCGAGCTCATATAGTTGGTATGTATTCTAATTCCAGACTACTGGCGTGGGATAAACATGTCCCCCTGATTAAAAGGAAAGATTCCATAGCCCATTAGTGCAAAGATAATTGGTACACCGTCAAAAACACATGGGAGACCACACGATACGACACTGTGACCGTAGCATCAATTCCGCACTCGATATAACTCTATCGAAGAACTTCCATGGTACAAACTGGTCGCCACGGTCTGCTCCACACGGAGATCATCGTATCATTTTTATCGATAGCGGCCGCTATCGATGTATGCCTTGGCCCTGATGGCATGCCAATTTCACTACAACGGACTATGTGGACCCCGTTATCATGGAAATGCGCTAGTTGGAGGAAGTTAGACCGCGCCGGAAAAGAGAGGGGTAGAGAAAATGACAACTTGGAAGATATCCACACACGCACGGTGAAACGTTAGCAACAATTATTAGGGAAGCACGCTTGCCCTAGTCCCACGAGTAACCAAGACTCCAAAAGCCTTTCTGGCACAGAGAGCGAGCCGAAATGGAAAAGAGAAACAATGCCTGCACTAACACTACTGAGCGATTCGCCTCGCCGCGGAGGACCGAATACTAATAACGACACTTGCGGTCAAAAAGTAGAAGAACAAATGCTCAACGATGAGTGAATCAGGTTAGGGTAGTTGGAAAATTATACAGAATGTCTTTGGCAACACACCGGTACCGTCATTGGGGTTAAGCGGAAAGGTGTGTGTCAGTAGGTTGTGAGGTGAAAGCGTTTTCAGATGCATAGTGAGCTTAATGTCCTTTTCACAGTATATCGTGTCTGATAGGTATCTCTTATTAGTATAGTCGAATACTAGTCAATAGTGCGTTTTGTGCAAAATGTCCATTTTGTGGCAGTGATGGGGTTGTTTTATGCTATTCCGTGTCTCTGGGTGGGCGTGCATTGAAAATAGGGGTTATCGGGTAGGGATCTCCCTATCAGTGATAGAGATCTCCCTATCAGTGATAGAGATCCCTGAGTACTGAGTTTAACATGTTCTCGTCCCGGGCTGCACGCGCCTTCGAGTTTTTTTTCCTTTTCCCCATTTTTTTCAACTTGAAGACTTCAATTACACCAAAAAGTAAAATTCACAAGCTTATGACAATCGCTGCACAGGGGGTTCCCTCCGGTCCCTCCGCCACGAAGCCGCTACAAGTGGCTTCATTGTACGTTGGCGACCTCGATCCTGCTATTAATGAGCCACAGTTGGTGGACATCTTTAAACCGTATGGAACTATTCTCAACGTCCGCGTCTGCCGTGACATCATCACGCAGCGCTCATTGGGATACGGTTATGTGAACTATGACGATGTGAATAGCGCGTCGAAAGCCATGGAGGAACTCAATTTCAAGCGAGTGGGTGAAAAGTGTATCCGAATCATGTGGCAGCAGCGTGACCCAGCACTGCGGTACAGCGGAAGTGGTAATATATTCGTCAAAAACCTTAAGGAGGAGGTAGACAGTCGGGAATTGAGTTTGATATTCAAGAAGTTTGGTGAGATCCTTTCCTGCAAGGTGATGGACGATGAGAGCGGGAAAAGTCGCGGTTACGGCTTTGTCCACTTCAAGGACGACGATGCAGCCAAAGCCGCCATCGAGAAAATGAACGGTGACAAGGAACACGCCGATGCGGATAAGGCAGCACTGTATGTTGCCAATTTCATTCGTCGTAATGCCCGTCTTGCCGCACTTGTGGCGAACTTCACGAACGTGTACATAAAGCAGCTCCTTCCCACAGTTGATAAAGACGTTATCGAAAAATTCTTTTCGAAGTTTGGCGGCATCACGAGCGCAGCGATATGCAAAGACAAGAACGGTCGTGCATTCGCCTTCTGTAACTTTGAAAAACACGATGACGCAGTTAAAGCCATTGAGGCGTCACATGATCAAGAGGTGGAAGGTGTTACGCAGCCTGGGGAGAAGTTGTACGTGCAGCGAGCTCAACCGAGAAGTGAGCGGCTTATTGCACTCCGCCAAAAATACATGCAGTGCCAGTCACTTGGAAACAACTTATACGTCCGTAACTTTGACGCTGAGTTCACGGAAAAAGACCTCAATGAACTGTTCAAAGAGTACGGTGTTATTCGCAGCTGCCGTGTGATGACCGATGCCAATGGCATAAGTCGCGGTTTCGGCTTTGTAAGTTTCGAAAATGCGGATCAGGCAAACGCCGCACTCCGTGAAATGAATGGTCGTATGCTGAACGGGAAACCTCTCGTTGTCAACATTGCGCAGCGCCGTGACCAGCGCCTCACCATGCTGAAGTTACAGCTCCAGCAACGCCTGCAAATGATGATGCACCATATGCATCCTCCTCCGTTCGGTATGCCGGGGCATCCACTGCAGAGACGCAATGCCCGCAGTGGCGGCCGGGGCAACCGTCCGCACCCACGTCAACAACAGCCACAACCTCAACCTGAGCCGCAACCACCACTCGCCACTACGGTGCCACCAGCAGCGGCTCACAGCGTTATGTTTACCGCACCCTCAATGGGTTTCTCCGCCGTACCCCGTACTCCTCAGGCATCACCTGCCATCGCTCCCGATACACCACCGCTGCCGCCCATCTCAGCTGAAGATCTGCAACAAATGTCGGTGGACGAACAACGCGCCGCTCTGGGTGACAGGCTCTACATTAAGGTGCATGAGCTTGCTCCGGATCACGCACCGAAAATTACTGGCATGTTCCTTGAGATGAACCCTAAGGAGGCGTTAGCCCTCCTTTCCAACCCTAAACTAATGCACGAGAAGGTTACAGAAGCCCTCTGTGTGCTAAAGGTACACGCCTCAAGCGCTTAAGATCCaacgagcaaaagctcatttctgaagaggacttgttaattaacgagcaaaagctcatttctgaagaggacttgTAACACCGGGTTGTGTGGCCAAAATTGTTCTGTAGTCGCTGTGAGTTGACACGGCTAGTGCTTATGATTTTCCTCGCGTGTGGTGCCTGTACTCAGCCCTATGCCTTATTTGCAACACATTTACGTACAGCGCACAAGAGAAGAGAAGATCACTTGAAGATAATAAATATAGGGTTGTAGGCATCTTGTTTAACTCAAATTTTCTCGTCTTGGTGTGTCGACATGATTGAAATAGTGCCACCAGTTGTGTTTGATGCGTTTGTTATCTATGCAGTATTCTGCAggGTTTAATTTGTTGGATGAGCTATTTCATTAATTTTTTTGCCTTCTCTTCTTTTGGGTTCGCTCGAGAGAGAGAGGGTGGGATTTTTTTCTTTAAATAAAGGGAGATGTATTCGGAGGAGTTGCATTAACGTTGTCCCATGTTTTACAAAAAACGAGTCTGAAAGAACGCCGACTCAATGgCCATCcAAGCGGAATAACCTTCTAAAAGAATCATATCCCTATTACCACACCAGTTTATATTACAGGGGAGGTTATTACAGAAATCTAGGATATCAGACTCAAGGTGTGGCGGACGTCTCGAACCGATCGTGAGTTGGCCCGGGCACAGCAAGGTCTTCTGAAATTCATGTTTTTTTTTTTTTTACTCTGCATTGCAGTCTCCGCTCTTATTTAGTTTTGCTTTACGTAAGGTCTCGTTGCTGCCATAAAATAAGCTctagaACTAGTGATGAAAAAGCCTGAACTCACCGCGACGTCTGTCGAGAAGTTTCTGATCGAAAAGTTCGACAGCGTCTCCGACCTGATGCAGCTCTCGGAGGGCGAAGAATCTCGTGCTTTCAGCTTCGATGTAGGAGGGCGTGGATATGTCCTGCGGGTAAATAGCTGCGCCGATGGTTTCTACAAAGATCGTTATGTTTATCGGCACTTTGCATCGGCCGCGCTCCCGATTCCGGAAGTGCTTGACATTGGGGAATTCAGCGAGAGCCTGACCTATTGCATCTCCCGCCGTGCACAGGGTGTCACGTTGCAAGACCTGCCTGAAACCGAACTGCCCGCTGTTCTGCAGCCGGTCGCGGAGGCCATGGATGCGATCGCTGCGGCCGATCTTAGCCAGACGAGCGGGTTCGGCCCATTCGGACCGCAAGGAATCGGTCAATACACTACATGGCGTGATTTCATATGCGCGATTGCTGATCCCCATGTGTATCACTGGCAAACTGTGATGGACGACACCGTCAGTGCGTCCGTCGCGCAGGCTCTCGATGAGCTGATGCTTTGGGCCGAGGACTGCCCCGAAGTCCGGCACCTCGTGCACGCGGATTTCGGCTCCAACAATGTCCTGACGGACAATGGCCGCATAACAGCGGTCATTGACTGGAGCGAGGCGATGTTCGGGGATTCCCAATACGAGGTCGCCAACATCTTCTTCTGGAGGCCGTGGTTGGCTTGTATGGAGCAGCAGACGCGCTACTTCGAGCGGAGGCATCCGGAGCTTGCAGGATCGCCGCGGCTCCGGGCGTATATGCTCCGCATTGGTCTTGACCAACTCTATCAGAGCTTGGTTGACGGCAATTTCGATGATGCAGCTTGGGCGCAGGGTCGATGCGACGCAATCGTCCGATCCGGAGCCGGGACTGTCGGGCGTACACAAATCGCCCGCAGAAGCGCGGCCGTCTGGACCGATGGCTGTGTAGAAGTACTCGCCGATAGTGGAAACCGACGCCCCAGCACTCGTCCGAGGGCAAAGGAATAGAGTAGATGCCGACCGGGATCGATCCCCCGATCCTAACACCGGGTTGTGTGGCCAAAATTGTTCTGTAGTCGCTGTGAGTTGACACGGCTAGTGCTTATGATTTTCCTCGCGTGTGGTGCCTGTACTCAGCCCTATGCCTTATTTGCAACACATTTACGTACAGCGCACAAGAGAAGAGAAGATCACTTGAAGATAATAAATATAGGGTTGTAGGCATCTTGTTTAACTCAAATTTTCTCGTCTTGGTGTGTCGACATGATTGAAATAGTGCCACCAGTTGTGTTTGATGCGTTTGTTATCTATGCAGTATTCTGCAAGGCCTTGCAAGGCCTTGCAGGCATGCAAGCTAGCTTGTATTCTATAGTGTCACCTAAATCGTATGTGTATGATACATAAGGTTATGTATTAATTGTAGCCGCGTTCTAACGACAATATGTACAAGCCTAATTGTGTAGCATCTGGCTTACTGAAGCAGACCCTATCATCTCTCTCGTAAACTGCCGTCAGAGTCGGTTTGGTTGGACGAACCTTCTGAGTTTCTGGTAACGCCGTTCCGCACCCCGGAAATGGTCAGCGAACCAATCAGCAGGGTCATCGCTAGCCAGATCCTCTACGCCGGACGCATCGTGGCCGGCATCACCGGCGCCACAGGTGCGGTTGCTGGCGCCTATATCGCCGACATCACCGATGGGGAAGATCGGGCTCGCCACTTCGGGCTCATGAGCGCTTGTTTCGGCGTGGGTATGGTGGCAGGCCCCGTGGCCGGGGGACTGTTGGGCGCCATCTCCTTGCACCATTCCTTGCGGCGGCGGTGCTCAACGGCCTCAACCTACTACTGGGCTGCTTCCTAATGCAGGAGTCGCATAAGGGAGAGCGTCGATATGGTGCACTCTCAGTACAATCTGCTCTGATGCCGCATAGTTAAGCCAGCCCCGACACCCGCCAACACCCGCTGACGCGCCCTGACGGGCTTGTCTGCTCCCGGCATCCGCTTACAGACAAGCTGTGACCGTCTCCGGGAGCTGCATGTGTCAGAGGTTTTCACCGTCATCACCGAAACGCGCGAGACGAAAGGGCCTCGTGATACGCCTATTTTTATAGGTTAATGTCATGATAATAATGGTTTCTTAGACGTCAGGTGGCACTTTTCGGGGAAATGTGCGCGGAACCCCTATTTGTTTATTTTTCTAAATACATTCAAATATGTATCCGCTCATGAGACAATAACCCTGATAAATGCTTCAATAATATTGAAAAAGGAAGAGTATGAGTATTCAACATTTCCGTGTCGCCCTTATTCCCTTTTTTGCGGCATTTTGCCTTCCTGTTTTTGCTCACCCAGAAACGCTGGTGAAAGTAAAAGATGCTGAAGATCAGTTGGGTGCACGAGTGGGTTACATCGAACTGGATCTCAACAGCGGTAAGATCCTTGAGAGTTTTCGCCCCGAAGAACGTTTTCCAATGATGAGCACTTTTAAAGTTCTGCTATGTGGCGCGGTATTATCCCGTATTGACGCCGGGCAAGAGCAACTCGGTCGCCGCATACACTATTCTCAGAATGACTTGGTTGAGTACTCACCAGTCACAGAAAAGCATCTTACGGATGGCATGACAGTAAGAGAATTATGCAGTGCTGCCATAACCATGAGTGATAACACTGCGGCCAACTTACTTCTGACAACGATCGGAGGACCGAAGGAGCTAACCGCTTTTTTGCACAACATGGGGGATCATGTAACTCGCCTTGATCGTTGGGAACCGGAGCTGAATGAAGCCATACCAAACGACGAGCGTGACACCACGATGCCTGTAGCAATGGCAACAACGTTGCGCAAACTATTAACTGGCGAACTACTTACTCTAGCTTCCCGGCAACAATTAATAGACTGGATGGAGGCGGATAAAGTTGCAGGACCACTTCTGCGCTCGGCCCTTCCGGCTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAACTGTCAGACCAAGTTTACTCATATATACTTTAGATTGATTTAAAACTTCATTTTTAATTTAAAAGGATCTAGGTGAAGATCCTTTTTGATAATCTCATGACCAAAATCCCTTAACGTGAGTTTTCGTTCCACTGAGCGTCAGACCCCGTAGAAAAGATCAAAGGATCTTCTTGAGATCCTTTTTTTCTGCGCGTAATCTGCTGCTTGCAAACAAAAAAACCACCGCTACCAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTCCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCGCAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGCCCAGCTTGGAGCGAACGACCTACACCGAACTGAGATACCTACAGCGTGAGCTATGAGAAAGCGCCACGCTTCCCGAAGGGAGAAAGGCGGACAGGTATCCGGTAAGCGGCAGGGTCGGAACAGGAGAGCGCACGAGGGAGCTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTCACATGTTCTTTCCTGCGTTATCCCCTGATTCTGTGGATAACCGTATTACCGCCTTTGAGTGAGCTGATACCGCTCGCCGCAGCCGAACGACCGAGCGCAGCGAGTCAGTGAGCGAGGAAGCGGAAGAGCGCCCAATACGCAAACCGCCTCTCCCCGCGCGTTGGCCGATTCATTAATGCAGCTGGCTTATCGAAATTAATACGACTCACTATAGGGAGACCGtcggtaccgtcatt
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