EPR Plasmid #222: pHD2454
Plasmid Details
| Purpose | Inducible expression of N-terminally CFP-tagged protein in Trypanosoma brucei, Gateway destination vector. Check reading frame before using, since the stored sequence has a termination codon after the CFP! Suggest Primer CGGCATGGACGAGCT |
| Depositing Lab |
Christine Clayton Heidelberg University |
| Depositing Scientist | Christine Clayton |
| Antibiotic Resistance | Ampicillin |
| Bacterial Growth Temp | 37 |
| Copy Number | High |
| Selectable Marker | Hygromycin |
| Reference |
Stress susceptibility in Trypanosoma brucei lacking the RNA-binding protein ZC3H30. Chakraborty C et al. PLoS Negl Trop Dis 2018 PubMed DOI See other plasmids from this publication |
| MTA and Terms |
MTA (PDF download) |
How to Cite This Reagent
IN THE MATERIALS & METHODS:
pHD2454 was a gift from Christine Clayton (European Plasmid Repository plasmid #222).
pHD2454 was a gift from Christine Clayton (European Plasmid Repository plasmid #222).
IN THE REFERENCES:
Chakraborty C et al.. Stress susceptibility in Trypanosoma brucei lacking the RNA-binding protein ZC3H30.. PLoS Negl Trop Dis 2018 doi:10.1371/journal.pntd.0006835 PubMed 30273340
Chakraborty C et al.. Stress susceptibility in Trypanosoma brucei lacking the RNA-binding protein ZC3H30.. PLoS Negl Trop Dis 2018 doi:10.1371/journal.pntd.0006835 PubMed 30273340
Sequences
Provided by depositor
download sequence
gaattcgagctcatatagttggtatgtattctaattccagactactggcgtggataaacatgtcccctgattaaaaggaaagattccatagcccattagtgcaaagataattggtacaccgtcaaaaacacatgggagaccacacgatacgacactgtgaccgtagcatcaattccgcactcgatataactctatcgaagaacttccatggtacaaactggtcgccacggtctgctccacacggagatcatcgtatcatttttatcgatagcggccgctatcgatgtatgccttggccctgatggcatgccaatttcactacaacggactatgtggaccccgttatcatggaaatgcgctagttggaggaagttagaccgcgccggaaaagagaggggtagagaaaatgacaacttggaagatatccacacacgcacggtgaaacgttagcaacaattattagggaagcacgcttgccctagtcccacgagtaaccaagactccaaaagcctttctggcacagagagcgagccgaaatggaaaagagaaacaatgcctgcactaacactactgagcgattcgcctcgccgcggaggaccgaatactaataacgacacttgcggtcaaaaagtagaagaacaaatgctcaacgatgagtgaatcaggttagggtagttggaaaattatacagaatgtctttggcaacacaccggtaccgtcattggggttaagcggaaaggtgtgtgtcagtaggttgtgaggtgaaagcgttttcagatgcatagtgagcttaatgtccttttcacagtatatcgtgtctgataggtatctcttattagtatagtcgaatactagtcaatagtgcgttttgtgcaaaatgtccattttgtggcagtgatggggttgttttatgctattccgtgtctctgggtgggcgtgcattgaaaataggggttatcgggtAGGGATCTCCCTATCAGTGATAGAGATCTCCCTATCAGTGATAGAGATCCCTgagtactgagtttaacatgttctcgtcccgggctgcacgcgccttcgagttttttttccttttccccatttttttcaacttgaagacttcaattacaccaaaaagtaaaattcacaagctacgcgtatcgttaacATGGTGAGCAAGGGCGAGGAGCTGTTCACCGGGGTGGTGCCCATCCTGGTCGAGCTGGACGGCGACGTAAACGGCCACAAGTTCAGCGTGTCCGGCGAGGGCGAGGGCGATGCCACCTACGGCAAGCTGACCCTGAAGTTCATCTGCACCACCGGCAAGCTGCCCGTGCCCTGGCCCACCCTCGTGACCACCCTGACCTACGGCGTGCAGTGCTTCAGCCGCTACCCCGACCACATGAAGCAGCACGACTTCTTCAAGTCCGCCATGCCCGAAGGCTACGTCCAGGAGCGCACCATCTTCTTCAAGGACGACGGCAACTACAAGACCCGCGCCGAGGTGAAGTTCGAGGGCGACACCCTGGTGAACCGCATCGAGCTGAAGGGCATCGACTTCAAGGAGGACGGCAACATCCTGGGGCACAAGCTGGAGTACAACTACAACAGCCACAACGTCTATATCATGGCCGACAAGCAGAAGAACGGCATCAAGGTGAACTTCAAGATCCGCCACAACATCGAGGACGGCAGCGTGCAGCTCGCCGACCACTACCAGCAGAACACCCCCATCGGCGACGGCCCCGTGCTGCTGCCCGACAACCACTACCTGAGCACCCAGTCCGCCCTGAGCAAAGACCCCAACGAGAAGCGCGATCACATGGTCCTGCTGGAGTTCGTGACCGCCGCCGGGATCACTCTCGGCATGGACGAGCTGTACAAGTAAaagcttatcaacaagtttgtacaaaaaagctgaacgagaaacgtaaaatgatataaatatcaatatattaaattagattttgcataaaaaacagactacataatactgtaaaacacaacatatccagtcactatggcggccgcattaggcaccccaggctttacactttatgcttccggctcgtataatgtgtggattttgagttaggatccgtcgagattttcaggagctaaggaagctaaaatggagaaaaaaatcactggatataccaccgttgatatatcccaatggcatcgtaaagaacattttgaggcatttcagtcagttgctcaatgtacctataaccagaccgttcagctggatattacggcctttttaaagaccgtaaagaaaaataagcacaagttttatccggcctttattcacattcttgcccgcctgatgaatgctcatccggaattccgtatggcaatgaaagacggtgagctggtgatatgggatagtgttcacccttgttacaccgttttccatgagcaaactgaaacgttttcatcgctctggagtgaataccacgacgatttccggcagtttctacacatatattcgcaagatgtggcgtgttacggtgaaaacctggcctatttccctaaagggtttattgagaatatgtttttcgtctcagccaatccctgggtgagtttcaccagttttgatttaaacgtggccaatatggacaacttcttcgcccccgttttcaccatgggcaaatattatacgcaaggcgacaaggtgctgatgccgctggcgattcaggttcatcatgccgtttgtgatggcttccatgtcggcagaatgcttaatgaattacaacagtactgcgatgagtggcagggcggggcgtaaagatctggatccggcttactaaaagccagataacagtatgcgtatttgcgcgctgatttttgcggtataagaatatatactgatatgtatacccgaagtatgtcaaaaagaggtatgctatgaagcagcgtattacagtgacagttgacagcgacagctatcagttgctcaaggcatatatgatgtcaatatctccggtctggtaagcacaaccatgcagaatgaagcccgtcgtctgcgtgccgaacgctggaaagcggaaaatcaggaagggatggctgaggtcgcccggtttattgaaatgaacggctcttttgctgacgagaacaggggctggtgaaatgcagtttaaggtttacacctataaaagagagagccgttatcgtctgtttgtggatgtacagagtgatattattgacacgcccgggcgacggatggtgatccccctggccagtgcacgtctgctgtcagataaagtctcccgtgaactttacccggtggtgcatatcggggatgaaagctggcgcatgatgaccaccgatatggccagtgtgccggtctccgttatcggggaagaagtggctgatctcagccaccgcgaaaatgacatcaaaaacgccattaacctgatgttctggggaatataaatgtcaggctcccttatacacagccagtctgcaggtcgaccatagtgactggatatgttgtgttttacagtattatgtagtctgttttttatgcaaaatctaatttaatatattgatatttatatcattttacgtttctcgttcagctttcttgtacaaagtggttgatgatcctaacaccgggttgtgtggccaaaattgttctgtagtcgctgtgagttgacacggctagtgcttatgattttcctcgcgtgtggtgcctgtactcagccctatgccttatttgcaacacatttacgtacagcgcacaagagaagagaagatcacttgaagataataaatatagggttgtaggcatcttgtttaactcaaattttctcgtcttggtgtgtcgacatgattgaaatagtgccaccagttgtgtttgatgcgtttgttatctatgcagtattctgcagtttaatttgttggatgagctatttcattaatttttttgccttctcttcttttgggttcgctcgagtttccacccagcgcgggtgcattctggctcttatatatacttattgtcatgacagagtatattgtactgtgttgataagggacgggtaactgtattgaagagccgatgcttttgacatgttagatataatatgttttattgtaaagtcaatacaacacacaataggataataatgataaagttaaaaaagtatatatagtaatagaaatatatcttatataggaaagattaagcagtaaaagtagcgcttacggcgtacggagcaggagagcaactgaccgctctcagaGCCCgggcacagcaaggtcttctgaaattcatgttttttttttttttactctgcattgcagtctccgctcttatttagttttgctttacgtaaggtctcgttgctgccataaaataagctactagtgatgaaaaagcctgaactcaccgcgacgtctgtcgagaagtttctgatcgaaaagttcgacagcgtctccgacctgatgcagctctcggagggcgaagaatctcgtgctttcagcttcgatgtaggagggcgtggatatgtcctgcgggtaaatagctgcgccgatggtttctacaaagatcgttatgtttatcggcactttgcatcggccgcgctcccgattccggaagtgcttgacattggggaattcagcgagagcctgacctattgcatctcccgccgtgcacagggtgtcacgttgcaagacctgcctgaaaccgaactgcccgctgttctgcagccggtcgcggaggccatggatgcgatcgctgcggccgatcttagccagacgagcgggttcggcccattcggaccgcaaggaatcggtcaatacactacatggcgtgatttcatatgcgcgattgctgatccccatgtgtatcactggcaaactgtgatggacgacaccgtcagtgcgtccgtcgcgcaggctctcgatgagctgatgctttgggccgaggactgccccgaagtccggcacctcgtgcacgcggatttcggctccaacaatgtcctgacggacaatggccgcataacagcggtcattgactggagcgaggcgatgttcggggattcccaatacgaggtcgccaacatcttcttctggaggccgtggttggcttgtatggagcagcagacgcgctacttcgagcggaggcatccggagcttgcaggatcgccgcggctccgggcgtatatgctccgcattggtcttgaccaactctatcagagcttggttgacggcaatttcgatgatgcagcttgggcgcagggtcgatgcgacgcaatcgtccgatccggagccgggactgtcgggcgtacacaaatcgcccgcagaagcgcggccgtctggaccgatggctgtgtagaagtactcgccgatagtggaaaccgacgccccagcactcgtccgagggcaaaggaatagagtagatgccgaccgggatcgatccaagctagcttgtattctatagtgtcacctaaatcgtatgtgtatgatacataaggttatgtattaattgtagccgcgttctaacgacaatatgtacaagcctaattgtgtagcatctggcttactgaagcagaccctatcatctctctcgtaaactgccgtcagagtcggtttggttggacgaaccttctgagtttctggtaacgccgttccgcaccccggaaatggtcagcgaaccaatcagcagggtcatcgctagccagatcctctacgccggacgcatcgtggccggcatcaccggcgccacaggtgcggttgctggcgcctatatcgccgacatcaccgatggggaagatcgggctcgccacttcgggctcatgagcgcttgtttcggcgtgggtatggtggcaggccccgtggccgggggactgttgggcgccatctccttgcaccattccttgcggcggcggtgctcaacggcctcaacctactactgggctgcttcctaatgcaggagtcgcataagggagagcgtcgatatggtgcactctcagtacaatctgctctgatgccgcatagttaagccagccccgacacccgccaacacccgctgacgcgccctgacgggcttgtctgctcccggcatccgcttacagacaagctgtgaccgtctccgggagctgcatgtgtcagaggttttcaccgtcatcaccgaaacgcgcgagacgaaagggcctcgtgatacgcctatttttataggttaatgtcatgataataatggtttcttagacgtcaggtggcacttttcggggaaatgtgcgcggaacccctatttgtttatttttctaaatacattcaaatatgtatccgctcatgagacaataaccctgataaatgcttcaataatattgaaaaaggaagagtatgagtattcaacatttccgtgtcgcccttattcccttttttgcggcattttgccttcctgtttttgctcacccagaaacgctggtgaaagtaaaagatgctgaagatcagttgggtgcacgagtgggttacatcgaactggatctcaacagcggtaagatccttgagagttttcgccccgaagaacgttttccaatgatgagcacttttaaagttctgctatgtggcgcggtattatcccgtattgacgccgggcaagagcaactcggtcgccgcatacactattctcagaatgacttggttgagtactcaccagtcacagaaaagcatcttacggatggcatgacagtaagagaattatgcagtgctgccataaccatgagtgataacactgcggccaacttacttctgacaacgatcggaggaccgaaggagctaaccgcttttttgcacaacatgggggatcatgtaactcgccttgatcgttgggaaccggagctgaatgaagccataccaaacgacgagcgtgacaccacgatgcctgtagcaatggcaacaacgttgcgcaaactattaactggcgaactacttactctagcttcccggcaacaattaatagactggatggaggcggataaagttgcaggaccacttctgcgctcggcccttccggctggctggtttattgctgataaatctggagccggtgagcgtgggtctcgcggtatcattgcagcactggggccagatggtaagccctcccgtatcgtagttatctacacgacggggagtcaggcaactatggatgaacgaaatagacagatcgctgagataggtgcctcactgattaagcattggtaactgtcagaccaagtttactcatatatactttagattgatttaaaacttcatttttaatttaaaaggatctaggtgaagatcctttttgataatctcatgaccaaaatcccttaacgtgagttttcgttccactgagcgtcagaccccgtagaaaagatcaaaggatcttcttgagatcctttttttctgcgcgtaatctgctgcttgcaaacaaaaaaaccaccgctaccagcggtggtttgtttgccggatcaagagctaccaactctttttccgaaggtaactggcttcagcagagcgcagataccaaatactgtccttctagtgtagccgtagttaggccaccacttcaagaactctgtagcaccgcctacatacctcgctctgctaatcctgttaccagtggctgctgccagtggcgataagtcgtgtcttaccgggttggactcaagacgatagttaccggataaggcgcagcggtcgggctgaacggggggttcgtgcacacagcccagcttggagcgaacgacctacaccgaactgagatacctacagcgtgagctatgagaaagcgccacgcttcccgaagggagaaaggcggacaggtatccggtaagcggcagggtcggaacaggagagcgcacgagggagcttccagggggaaacgcctggtatctttatagtcctgtcgggtttcgccacctctgacttgagcgtcgatttttgtgatgctcgtcaggggggcggagcctatggaaaaacgccagcaacgcggcctttttacggttcctggccttttgctggccttttgctcacatgttctttcctgcgttatcccctgattctgtggataaccgtattaccgcctttgagtgagctgataccgctcgccgcagccgaacgaccgagcgcagcgagtcagtgagcgaggaagcggaagagcgcccaatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcttatcgaaattaatacgactcactatagggagaccg
gaattcgagctcatatagttggtatgtattctaattccagactactggcgtggataaacatgtcccctgattaaaaggaaagattccatagcccattagtgcaaagataattggtacaccgtcaaaaacacatgggagaccacacgatacgacactgtgaccgtagcatcaattccgcactcgatataactctatcgaagaacttccatggtacaaactggtcgccacggtctgctccacacggagatcatcgtatcatttttatcgatagcggccgctatcgatgtatgccttggccctgatggcatgccaatttcactacaacggactatgtggaccccgttatcatggaaatgcgctagttggaggaagttagaccgcgccggaaaagagaggggtagagaaaatgacaacttggaagatatccacacacgcacggtgaaacgttagcaacaattattagggaagcacgcttgccctagtcccacgagtaaccaagactccaaaagcctttctggcacagagagcgagccgaaatggaaaagagaaacaatgcctgcactaacactactgagcgattcgcctcgccgcggaggaccgaatactaataacgacacttgcggtcaaaaagtagaagaacaaatgctcaacgatgagtgaatcaggttagggtagttggaaaattatacagaatgtctttggcaacacaccggtaccgtcattggggttaagcggaaaggtgtgtgtcagtaggttgtgaggtgaaagcgttttcagatgcatagtgagcttaatgtccttttcacagtatatcgtgtctgataggtatctcttattagtatagtcgaatactagtcaatagtgcgttttgtgcaaaatgtccattttgtggcagtgatggggttgttttatgctattccgtgtctctgggtgggcgtgcattgaaaataggggttatcgggtAGGGATCTCCCTATCAGTGATAGAGATCTCCCTATCAGTGATAGAGATCCCTgagtactgagtttaacatgttctcgtcccgggctgcacgcgccttcgagttttttttccttttccccatttttttcaacttgaagacttcaattacaccaaaaagtaaaattcacaagctacgcgtatcgttaacATGGTGAGCAAGGGCGAGGAGCTGTTCACCGGGGTGGTGCCCATCCTGGTCGAGCTGGACGGCGACGTAAACGGCCACAAGTTCAGCGTGTCCGGCGAGGGCGAGGGCGATGCCACCTACGGCAAGCTGACCCTGAAGTTCATCTGCACCACCGGCAAGCTGCCCGTGCCCTGGCCCACCCTCGTGACCACCCTGACCTACGGCGTGCAGTGCTTCAGCCGCTACCCCGACCACATGAAGCAGCACGACTTCTTCAAGTCCGCCATGCCCGAAGGCTACGTCCAGGAGCGCACCATCTTCTTCAAGGACGACGGCAACTACAAGACCCGCGCCGAGGTGAAGTTCGAGGGCGACACCCTGGTGAACCGCATCGAGCTGAAGGGCATCGACTTCAAGGAGGACGGCAACATCCTGGGGCACAAGCTGGAGTACAACTACAACAGCCACAACGTCTATATCATGGCCGACAAGCAGAAGAACGGCATCAAGGTGAACTTCAAGATCCGCCACAACATCGAGGACGGCAGCGTGCAGCTCGCCGACCACTACCAGCAGAACACCCCCATCGGCGACGGCCCCGTGCTGCTGCCCGACAACCACTACCTGAGCACCCAGTCCGCCCTGAGCAAAGACCCCAACGAGAAGCGCGATCACATGGTCCTGCTGGAGTTCGTGACCGCCGCCGGGATCACTCTCGGCATGGACGAGCTGTACAAGTAAaagcttatcaacaagtttgtacaaaaaagctgaacgagaaacgtaaaatgatataaatatcaatatattaaattagattttgcataaaaaacagactacataatactgtaaaacacaacatatccagtcactatggcggccgcattaggcaccccaggctttacactttatgcttccggctcgtataatgtgtggattttgagttaggatccgtcgagattttcaggagctaaggaagctaaaatggagaaaaaaatcactggatataccaccgttgatatatcccaatggcatcgtaaagaacattttgaggcatttcagtcagttgctcaatgtacctataaccagaccgttcagctggatattacggcctttttaaagaccgtaaagaaaaataagcacaagttttatccggcctttattcacattcttgcccgcctgatgaatgctcatccggaattccgtatggcaatgaaagacggtgagctggtgatatgggatagtgttcacccttgttacaccgttttccatgagcaaactgaaacgttttcatcgctctggagtgaataccacgacgatttccggcagtttctacacatatattcgcaagatgtggcgtgttacggtgaaaacctggcctatttccctaaagggtttattgagaatatgtttttcgtctcagccaatccctgggtgagtttcaccagttttgatttaaacgtggccaatatggacaacttcttcgcccccgttttcaccatgggcaaatattatacgcaaggcgacaaggtgctgatgccgctggcgattcaggttcatcatgccgtttgtgatggcttccatgtcggcagaatgcttaatgaattacaacagtactgcgatgagtggcagggcggggcgtaaagatctggatccggcttactaaaagccagataacagtatgcgtatttgcgcgctgatttttgcggtataagaatatatactgatatgtatacccgaagtatgtcaaaaagaggtatgctatgaagcagcgtattacagtgacagttgacagcgacagctatcagttgctcaaggcatatatgatgtcaatatctccggtctggtaagcacaaccatgcagaatgaagcccgtcgtctgcgtgccgaacgctggaaagcggaaaatcaggaagggatggctgaggtcgcccggtttattgaaatgaacggctcttttgctgacgagaacaggggctggtgaaatgcagtttaaggtttacacctataaaagagagagccgttatcgtctgtttgtggatgtacagagtgatattattgacacgcccgggcgacggatggtgatccccctggccagtgcacgtctgctgtcagataaagtctcccgtgaactttacccggtggtgcatatcggggatgaaagctggcgcatgatgaccaccgatatggccagtgtgccggtctccgttatcggggaagaagtggctgatctcagccaccgcgaaaatgacatcaaaaacgccattaacctgatgttctggggaatataaatgtcaggctcccttatacacagccagtctgcaggtcgaccatagtgactggatatgttgtgttttacagtattatgtagtctgttttttatgcaaaatctaatttaatatattgatatttatatcattttacgtttctcgttcagctttcttgtacaaagtggttgatgatcctaacaccgggttgtgtggccaaaattgttctgtagtcgctgtgagttgacacggctagtgcttatgattttcctcgcgtgtggtgcctgtactcagccctatgccttatttgcaacacatttacgtacagcgcacaagagaagagaagatcacttgaagataataaatatagggttgtaggcatcttgtttaactcaaattttctcgtcttggtgtgtcgacatgattgaaatagtgccaccagttgtgtttgatgcgtttgttatctatgcagtattctgcagtttaatttgttggatgagctatttcattaatttttttgccttctcttcttttgggttcgctcgagtttccacccagcgcgggtgcattctggctcttatatatacttattgtcatgacagagtatattgtactgtgttgataagggacgggtaactgtattgaagagccgatgcttttgacatgttagatataatatgttttattgtaaagtcaatacaacacacaataggataataatgataaagttaaaaaagtatatatagtaatagaaatatatcttatataggaaagattaagcagtaaaagtagcgcttacggcgtacggagcaggagagcaactgaccgctctcagaGCCCgggcacagcaaggtcttctgaaattcatgttttttttttttttactctgcattgcagtctccgctcttatttagttttgctttacgtaaggtctcgttgctgccataaaataagctactagtgatgaaaaagcctgaactcaccgcgacgtctgtcgagaagtttctgatcgaaaagttcgacagcgtctccgacctgatgcagctctcggagggcgaagaatctcgtgctttcagcttcgatgtaggagggcgtggatatgtcctgcgggtaaatagctgcgccgatggtttctacaaagatcgttatgtttatcggcactttgcatcggccgcgctcccgattccggaagtgcttgacattggggaattcagcgagagcctgacctattgcatctcccgccgtgcacagggtgtcacgttgcaagacctgcctgaaaccgaactgcccgctgttctgcagccggtcgcggaggccatggatgcgatcgctgcggccgatcttagccagacgagcgggttcggcccattcggaccgcaaggaatcggtcaatacactacatggcgtgatttcatatgcgcgattgctgatccccatgtgtatcactggcaaactgtgatggacgacaccgtcagtgcgtccgtcgcgcaggctctcgatgagctgatgctttgggccgaggactgccccgaagtccggcacctcgtgcacgcggatttcggctccaacaatgtcctgacggacaatggccgcataacagcggt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