EPR Plasmid #35: pAc5.1-dTsc2[S1046A]-V5-His
Plasmid Details
| Purpose | Constitutive expression in insect cell culture of Drosophila full-length TSC2[S1046A] with V5 and HIS epitope tags |
| Depositing Lab |
Aurelio Teleman Deutsches Krebsforschungszentrum (DKFZ) |
| Depositing Scientist | Aurelio Teleman |
| Antibiotic Resistance | Ampicillin |
| Bacterial Growth Temp | 37 |
| Copy Number | High |
| Selectable Marker | |
| Retransformation Info: | Retransforms well in XL1blue. |
| Reference |
CycD/Cdk4 and Discontinuities in Dpp Signaling Activate TORC1 in the Drosophila Wing Disc. Romero-Pozuelo J et al. Dev Cell 2017 PubMed DOI See other plasmids from this publication |
| MTA and Terms |
MTA (PDF download) |
How to Cite This Reagent
IN THE MATERIALS & METHODS:
pAc5.1-dTsc2[S1046A]-V5-His was a gift from Aurelio Teleman (European Plasmid Repository plasmid #35).
pAc5.1-dTsc2[S1046A]-V5-His was a gift from Aurelio Teleman (European Plasmid Repository plasmid #35).
IN THE REFERENCES:
Romero-Pozuelo J et al.. CycD/Cdk4 and Discontinuities in Dpp Signaling Activate TORC1 in the Drosophila Wing Disc.. Dev Cell 2017 doi:10.1016/j.devcel.2017.07.019 PubMed 28829945
Romero-Pozuelo J et al.. CycD/Cdk4 and Discontinuities in Dpp Signaling Activate TORC1 in the Drosophila Wing Disc.. Dev Cell 2017 doi:10.1016/j.devcel.2017.07.019 PubMed 28829945
Sequences
Provided by depositor
download sequence
CTTCAAGGAATTAATTCTATATTCTAAAAACACAAATGATACTTCTAAAAAAAATCATGAATGGCATCAACTCTGAATCAAATCTTTGCAGATGCACCTACTTCTCATTTCCACTGTCACATCATTTTTCCAGATCTCGCTGCCTGTTATGTGGCCCACAAACCAAGACACGTTTTATGGCCATTAAAGCTGGCTGATCGTCGCCAAACACCAAATACATATCAATATGTACATTCGAGAAAGAAGCGATCAAAGAAGCGTCTTCGGGCGAGTAGGAGAATGCGGAGGAGAAGGAGAACGAGCTGATCTAGTATCTCTCCACAATCCAATGCCAACTGACCAACTGGCCATATTCGGAGCAATTTGAAGCCAATTTCCATCGCCTGGCGATCGCTCCATTCTTGGCTATATGTTTTTCACCGTTCCCGGGGCCATTTTCAAAGACTCGTCGGTAAGATAAGATTGTGTCACTCGCTGTCTCTCTTCATTTGTCGAAGAATGCTGAGGAATTTCGCGATGACGTCGGCGAGTATTTTGAAGAATGAGAATAATTTGTATTTATACGAAAATCAGTTAGTGGAATTTTCTACAAAAACATGTTATCTATAGATAATTTTGTTGCAAAATATGTTGACTATGACAAAGATTGTATGTATATACCTTTAATGTATTCTCATTTTCTTATGTATTTATAATGGCAATGATGATACTGATGATATTTTAAGATGATGCCAGACCACAGGCTGATTTCTGCGTCTTTTGCCGAACGCAGTGCATGTGCGGTTGTTGTTTTTTGGAATAGTTTCAATTTTCGGACTGTCCGCTTTGATTTCAGTTTCTTGGCTTATTCAAAAAGCAAAGTAAAGCCAAAAAAGCGAGATGGCAATACCAAATGCGGCAAAACGGTAGTGGAAGGAAAGGGGTGCGGGGCAGCGGAAGGAAGGGTGGGGCGGGGCGTGGCGGGGTCTGTGGCTGGGCGCGACGTCACCGACGTTGGAGCCACTCCTTTGACCATGTGTGCGTGTGTGTATTATTCGTGTCTCGCCACTCGCCGGTTGTTTTTTTCTTTTTATCTCGCTCTCTCTAGCGCCATCTCGTACGCATGCTCAACGCACCGCATGTTGCCGTGTCCTTTATGCGTCATTTTGGCTCGAAATAGGCAATTATTTAAACAAAGATTAGTCAACGAAAACGCTAAAATAAATAAGTCTACAATATGGTTACTTATTGCCATGTGTGTGCAGCCAACGATAGCAACAAAAGCAACAACACAGTGGCTTTCCCTCTTTCACTTTTTGTTTGCAAGCGCGTGCGAGCAAGACGGCACGACCGGCAAACGCAATTACGCTGACAAAGAGCAGACGAAGTTTTGGCCGAAAAACATCAAGGCGCCTGATACGAATGCATTTGCAATAACAATTGCGATATTTAATATTGTTTATGAAGCTGTTTGACTTCAAAACACACAAAAAAAAAAATAAAACAAATTATTTGAAAGAGAATTAGGAATCGGACAGCTTATCGTTACGGGCTAACAGCACACCGAGACGAAATAGCTTACCTGACGTCACAGCCTCTGGAAGAACTGCCGCCAAGCAGAGAGAGAGAGAAAAAGAGGGAGAGCAGCTTAGACCGCATGTGCTTGTGTGTGAGGCGTCTCTCTCTTCGTCTCCTGTTTGCGCAAACGCATAGACTGCACTGAGAAAATCGATTACCTATTTTTTATGAATGAATATTTGCACTATTACTATTCAAAACTATTAAGATAGCAATCACATTCAATAGCCAAATACTATACCACCTGAGCGATGCAACGAAATGATCAATTTGAGCAAAAATGCTGCATATTTAGGACGGCATCATTATAGAAATGCTTCTTGCTGTGTACTTTTCTCTCGTCTGGCAGCTGTTTCGCCGTTATTGTTAAAACCGGCTTAAGTTAGGTGTGTTTTCTACGACTAGTGATGCCCCTACTAGAAGATGTGTGTTGCACAAATGTCCCTGAATAACCAATTTGAAGTGCAGATAGCAGTAAACGTAAGCTAATATGAATATTATTTAACTGTAATGTTTTAATATCGCTGGACATTACTAATAAACCCACTATAAACACATGTACATATGTATGTTTTGGCATACAATGAGTAGTTGGGGAAAAAATGTGTAAAAGCACCGTGACCATCACAGCATAAAGATAACCAGCTGAAGTATCGAATATGAGTAACCCCCAAATTGAATCACATGCCGCAACTGATAGGACCCATGGAAGTACACTCTTCATGGCGATATACAAGACACACACAAGCACGAACACCCAGTTGCGGAGGAAATTCTCCGTAAATGAAAACCCAATCGGCGAACAATTCATACCCATATATGGTAAAAGTTTTGAACGCGACTTGAGAGCGGAGAGCATTGCGGCTGATAAGGTTTTAGCGCTAAGCGGGCTTTATAAAACGGGCTGCGGGACCAGTTTTCATATCACTACCGTTTGAGTTCTTGTGCTGTGTGGATACTCCTCCCGACACAAAGCCGCTCCATCAGCCAGCAGTCGTCTAATCCAGAGACcccggatcggggtacctactagtccagtgtggtggaattctgcagatatccagcacagtggcggccgcTCTAGAactagtggatcccccgggctgcaggaattcggcacgaggtgacttcctctgttctgtgctgtgtataaattgtttttaggacctaggctaggaattactggttgcacacactcagcgccacagctccaccgaccaccgcccttgcaaggacccaccATGAACTCCAAGGACAAGTCCAAGTTCAAGTTGTTCCTCAAATCGCTGCCGGCAGGTTACGTGGGCGAGCGGACCCTGCGGCCGGAGTTTGAGAGGGAACTGCGTCCGGAGCAGCCGGTGGCCCAGCGCTGCCGGATGCTGAAAGAGCTGGGCGACACGCAGCTGCACAACTTCAATCTGGACGAGTCCGCCATCACCATTCTGTTCAATCTCACCAACGATCTCATCGTGCCCAACAAGCCGGCAGAAACGCGGCAGATTGCCTTGAGCTTCTATAAACGGCTCATTCACACACAGTACAAGAACCTGACCATCATGCGGGAGAAGTTCTTCCTGGTCATCCAGAACCACGAAGCCCGCGAAGATCTGCGTCACCTTCTTGAGCTGCTCGACACCCTCACCGACAATGGGAAGGATATCACAAACTTCGAGGAGAAGATTGGCAAGTTTATGCTGCTCTGGATCCCAGCCATTACGGAGGCCAATCTGCTTACCCCCTACCTTGACATTCTGGTCAACCTGATCAAGTTCAATGCGGCGCATCTGGACAAAGACATCCTTGTGGGCATCGTGCAGAACGCTTGCGATCTGAGCTGTAGCGTTACCGTAAACGAAATCGGTCTGCAGTGCCTCACAATTCTGGAGATGGTCATCGGGTACACCATCTTCCCAAGCGAACCGCTGCCGCAGTGCATCACCACCTTGTGCCGGACTGTGAATCATGCTCCTTACTGCCCGTCTTCTTTCAAGATCATGAAGAACCTCTTGGGCACCCAGTTGGGATACCATTCCATGAAGATGATGTGCAGTATCCTCAACGATCGAGCTCTTTACGATGATGCCCACCTGTTGCGTGGGGCCGTGTTCCATCTAAACATGAACATCTTTGGCTCGAACATTATTTTCCAGGTTTCGCCTATGACCTATGCAACGAATGTGCTGACAGCCTTCCTGCGGGCACTAGACAGTCGTCAGGTGATCGTGAccttcgaagtcatcctcagcGTTCGCATGGTGATTACCAAGCGGCAGCTTTCAGAGATCATTTGGGATCTGATCTGCGATATAATGTCCTCGATCGTTAGCAATATCGAGTATTACGAAGAAGTCAATATAAACAAGGATCGTCTGCATCACCTGCAGATCAATTTCCACGAGAATATCGACTGCATCGAGAAGCTGTTGCAGCAGGATAGATCCCAGATTCTGGGCAATGTGGAGCGCATCTACGATCTCATCGAGCGAGTTGCCGACCGACGCCCAGAGGCTTCCGTTCTAGCCCTAATCGAGTACCGATCCCGACGGGTTACAGCAACGCGGCCAGATTGGCTTCAGGTGCTGGCCCAGTTCGTCCGGCGCTACtaccgcatgtccaacgttaaCGTGCGCATTAAGACGATCGAGGCTCTAGTTCAGATTATGGATCAAAACCGTGCCTGCTATGAAGAAGAAATCCTTAGTCGCGTGGTGCTGGTGCACCTTTCCCATATTCATCTGGAGTCGAGTGTGCAGGTGCGAGTGGCCGTGGCCCGGGCTCTTTCCAATTTTGCCACCCATTGCGACACGAAACGGTGCATGGATCTGCTAGATATCCTGGAGGCGCTCATCAATCGACCCTTTGAGCACACGCGGCATGGATCCTCATCTGGAGAGGGTACACTTGCAGAAGTGACCTTTGGATTGGTCAACAACGAATCCGAGATCTCGGATATTATCGCCGCAGTTGATGGTTTGGTCAAAGTGTTTGCCATCAAGCTGCATCGTCTGCCAGGGATCCATGCTCTTAAAATATTTAACATTCTAATGGACCACCTGGAACTGCACTACGAACGACCAAAGATTTTCGAGCACATAAGTGTTGTGCGCTATAAAATATTTGCTTGGTTGCTGAAGGCCCGGGCCAACGGCTCCTACCATATTGGTTATCCGGAGGGCAGCACCGAGGTGGTGAAATTTAGTCCATACCTGGGAATTGATTCACCACTGTTACCACAAGCCCACCCGACAGCGATATCCATACGACGAGTCTGCAAGCTGATAGTGAGGTGCCTGGAACACGACACCGATTATCAGGTGTTCCAGCTAGTCATCAGGGAACTGCCGAAGGTGCTGCAGAACAAGGCGCTGGTGCAGGGCAATGACATCGAGGAGCTGGCCAACACGCTCCTCAAGATCAATTTGGTTAGCAACAACAAGTTCAAGCGACCCACCGATGAGTTCCACGCCCTCGTTTTACCCGCCATAGCTTCATTGGTTATCTATCACGAAAGCCTGCAGCCGCAGCAGCACTACGGAATTATAACAGCTCTCAATTCGAGGGTACTTACTGGCATTGCCAGCGTCTGCATCAACACCATGACCATCCTTATACTAGAGATGCCGGAGGCGTTGATGCGAAAGCTGCCGGACGTACTGCTCCAAATGAGTAAGATGTCGGATACGAATGCGTTGGCCACTCCGGTTCTGGAATTTCTGTCGTTGCTCATACATCTGTCCAAGCATCTCTTCGCAAACTTCACTTCCATGCACAACATGTACGTGTTTGCCATCACGCTGCCGTACACCAAACCGCATCGCTACGATCACTATACAGTTTCCTTGGCCCACCATGTCATCGCTGGATGGTTCCTTAAGTGTAAGCTGGAGCTGCGCAGGAACTGCGTTAACTACATCAAAAGCTCGATTCAGTCGAATGCCAAGATGTTGTCCAGCGACATTGTGAACCTAAACTCCTTAAACGAGGATTCATCCAATCGCAAGCGGAGCACCAGTCTAACTGAGCGCGGTAGTCGCAACAATGCCAATGCCTGGAACGATCTGGAGATGCGACCGCAAATGAACAACGGTCTACGAAACTTCCACGCCGAACTGGCAGAGACGTGCTTTGATTTCTTGGCGCGACACACTTATTCGCCTTGTCCGTCGATGCCCAAGCGTCTTCCAGCGGCGGAATTCCTGCTGAAGGACGGCGTCTCGCAGACGTGGCTAGTGGGCAACAACCTGGTGACCATAACCACCTCCGGCTGTCCGTCGGCGCCCGCGCGCAGTGGGCTTTGCGAACGATGTGCCCAGCTAGGAAAGGCTCCCAGCATTAGTCTGAACTCGAAGAGCCTCAGTGATGCAGCTCCTCCACTTgCGCCAGAGCGGGAGAGACGATACACCAAGGTGAGCCTGCAGCACAGCAGCGGCAACGAGTCGGCTGGCAGCACGGAGCTCACCTCCTCCTCGTCGTCGAACTCAGCAGCCGCAGGTGGTCATCCACATCGACAGATTTCTAACAGTTCCACGGCATCGCTAGACGCGCTTTCCCGTCGGGGGTCTAATCCCGAAGCCTTGGGCAGTGCCTTGGGCGAGGGGGCACATACGGGGAGCAACACCTCGCTCCTGGGAAACTCTTTGTCCCAATCCTCCGTTAGCATGAGCCCTTCGTCGGGATCCGTGGTGCAGCAGCCGGTTTGCGTGCGCGCCTGCACTGGCTGGGCGGAGGTTTTGGTTCGTCGGCCCACGGGCAACGTATCGTGGATAACCCGCATTCAGAACCCCATCACCAACGATTGCTTTGGCCAGGAGTTGCCGTTCAATAATGTGGTGTCGCTGTTTCTTCCCACTGCCCATGGCGGAGTTTTTGGACCTGACAATACCATTCAGGCCCCACCACATACGTTGGCTGATCCCGAACCAGAACCAGAAGCAGAGGTTAATGCAGCAACAGCTCCTCAGGCAAGTGCATTGCGCAGCCGAATGGTTGCCAAGGCTCGTGCCCTTCAGCGACAGGAAGAAATTCACTCGGTCGGAGGAAATGGAAATGGCAACGGCAACGGTAATGGTAGTGGAAATGCTCCCAGCACTGGAGGTGCTGCTGCCATACCCATTCCGCGGGTCCCAGCATCTGGAAAACGCGGCAAGGAGGCAGCTCTTTgtggaagtgtgagtgatggTGAAGCCGACGATGACTCCCTGGCCTTTGAGGATGCTCAGAGTCGGGCACGAAATCCCGTCCGACGGGTAAATTCCAGCCCAGAGATGAGTTCCAGTTGGCGGCAGTCGTTCCTAACCAACAAACCCACGCCACTGTCCCAGGAGCCCGTGAAAACCACCGCCGATGAACCGCAATCGCTGTTGACTTTGAAGAAGAAGACTGTTCAGTACAGCACCAAGGATATGCGTGTGAGCTGTGAGGCCATTCCGGAGGAGATCGCCGGCTCCACACCACCGTCGCAGGCCGCAGCCTTGGCCCTGCAGCCGGAAAGCGGCACCCTACCTCCCAAGCAGCATTCGGCGGACGATGTCAGCAGTCATGTGGCTGGAGGAAGTAACTTGCAAGCAGGAGGGTCCACGCTAAAGCTGGGTAAACCACCGCTGTCGCCGGGGCAACCGCCTCTGCTGGGCACTGGTCGAGTGACCAGCTTTGGTGGCACCTCTGTTCCGCAGCCCGGTGCACTGGCAAAATCGAGCAGCAGTGGCAGCAATGGCACCAATGTTGGAGTGATAACCTCCGATTACGACAACGGCAACAATGGAAACGGAGACATGATGCGAGGTCGTTCGAAAACCATTTCGGTGGTGCGTGAAGTGAATAACGGAAATACGCGTCCGCCGCCGGCCAGCAGTTTCCGGAATTTCGGAGCCGCAAAGCCGCCCATCAACACCAAGCTGTGCATGAACCCGAGCTTTATTTTCATGCAACTGTACACCACTGGCCAGCTGGGAGTTACGGATGAGCCTCTAAAGGTGGGTCCGGAAAACAGCAGCGCTGTCTCCCTTATCGATTTAGTCCCACCGTTCGAGACGCACAAGATCGGCGTGCTGTACGTGGGCCAAGGGCAGTGCAACAACGAGGTGGAGATCTTGCGCAATTCGCACGGAAGTGCCAGGTATGTGGAGTTCCTGCGTAAcatcggcaccctggtcagtctgAAGGAAGCTGAGCAGAACAATCTATTTATAATGCTAGATAGAAACGGAGCAGATGGCAAGTTTGCCTATATCTGGAAGGATGACATACTGCAGGTTACGTTCCATGTCGCTACGCTGATGCCCACCAATCTGCAGGATGATCCCAACTGCAACGAGAAGAAGAGCCACATTGGCAATGACTTTGTGAAGATCATCTACAACGAAAgcggcgaggagtacaatctaaacacAATATCCGGCCAATTCAACTACGCCTGCGTGATCGTTGAGCCACTTGACCTGAACTCGAACAGGGTGTATGTGAAGGCGCGATCGGAGATTTCGAAATTCGTGTGCCACGCAGAATACCGGATAGTGTCCGATCGCAGTGCTCCTCTTTTGGCCCGACAGATGGCGCTGCACGccaatctcgcgtcgctagtctacCAAAGTGTGCAGAAAAAGCATCCGTACGCATCGAATTGGCTAGAACGATTGCGCAAACTAAAGCGCCTGCGATCGAAGCTAATTGAGGGCCTAAACAGCCAGCAAAAATCCGGAAGCGCCTCGAGcggcatcggaatcaacgcctcagccattggttcggatatggacgaccagcgaggtgacttcattaaatacacagagggcccgcggttcgaaggtaagcctatccctaaccctctcctcggtctcgattctacgcgtaccggtcatcatcaccatcaccattgagtttaaacccgctgatcagcctcgactgtgccttctaaggcctgagctcgcTGATCAGCCTCGATCGAGGATCCAGACATGATAAGATACATTGATGAGTTTGGACAAACCACAACTAGAATGCAGTGAAAAAAATGCTTTATTTGTGAAATTTGTGATGCTATTGCTTTATTTGTAACCATTATAAGCTGCAATAAACAAGTTAACAACAACAATTGCATTCATTTTATGTTTCAGGTTCAGGGGGAGGTGTGGGAGGTTTTTTAAAGCAAGTAAAACCTCTACAAATGTGGTATGGCTGATTATGATCAGTCGACCGATGCCCTTGAGAGCCTTCAACCCAGTCAGCTCCTTCCGGTGGGCGCGGGGCATGACTATCGTCGCCGCACTTATGACTGTCTTCTTTATCATGCAACTCGTAGGACAGGTGCCGGCAGCGCTCTGGGTCATTTTCGGCGAGGACCGCTTTCGCTGGAGCGCGACGATGATCGGCCTGTCGCTTGCGGTATTCGGAATCTTGCACGCCCTCGCTCAAGCCTTCGTCACTGGTCCCGCCACCAAACGTTTCGGCGAGAAGCAGGCCATTATCGCCGGCATGGCGGCCGACGCGCTGGGCTACGTCTTGCTGGCGTTCGCGACGCGAGGCTGGATGGCCTTCCCCATTATGATTCTTCTCGCTTCCGGCGGCATCGGGATGCCCGCGTTGCAGGCCATGCTGTCCAGGCAGGTAGATGACGACCATCAGGGACAGCTTCAAGGATCGCTCGCGGCTCTTACCAGCCAGCAAAAGGCCAGGAACCGTAAAAAGGCCGCGTTGCTGGCGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCGACGCTCAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCCTGGAAGCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCTTTCTCCCTTCGGGAAGCGTGGCGCTTTCTCAATGCTCACGCTGTAGGTATCTCAGTTCGGTGTAGGTCGTTCGCTCCAAGCTGGGCTGTGTGCACGAACCCCCCGTTCAGCCCGACCGCTGCGCCTTATCCGGTAACTATCGTCTTGAGTCCAACCCGGTAAGACACGACTTATCGCCACTGGCAGCAGCCACTGGTAACAGGATTAGCAGAGCGAGGTATGTAGGCGGTGCTACAGAGTTCTTGAAGTGGTGGCCTAACTACGGCTACACTAGAAGGACAGTATTTGGTATCTGCGCTCTGCTGAAGCCAGTTACCTTCGGAAAAAGAGTTGGTAGCTCTTGATCCGGCAAACAAACCACCGCTGGTAGCGGTGGTTTTTTTGTTTGCAAGCAGCAGATTACGCGCAGAAAAAAAGGATCTCAAGAAGATCCTTTGATCTTTTCTACGGGGTCTGACGCTCAGTGGAACGAAAACTCACGTTAAGGGATTTTGGTCATGAGATTATCAAAAAGGATCTTCACCTAGATCCTTTTAAATTAAAAATGAAGTTTTAAATCAATCTAAAGTATATATGAGTAAACTTGGTCTGACAGTTACCAATGCTTAATCAGTGAGGCACCTATCTCAGCGATCTGTCTATTTCGTTCATCCATAGTTGCCTGACTCCCCGTCGTGTAGATAACTACGATACGGGAGGGCTTACCATCTGGCCCCAGTGCTGCAATGATACCGCGAGACCCACGCTCACCGGCTCCAGATTTATCAGCAATAAACCAGCCAGCCGGAAGGGCCGAGCGCAGAAGTGGTCCTGCAACTTTATCCGCCTCCATCCAGTCTATTAATTGTTGCCGGGAAGCTAGAGTAAGTAGTTCGCCAGTTAATAGTTTGCGCAACGTTGTTGCCATTGCTGCAGGCATCGTGGTGTCACGCTCGTCGTTTGGTATGGCTTCATTCAGCTCCGGTTCCCAACGATCAAGGCGAGTTACATGATCCCCCATGTTGTGCAAAAAAGCGGTTAGCTCCTTCGGTCCTCCGATCGTTGTCAGAAGTAAGTTGGCCGCAGTGTTATCACTCATGGTTATGGCAGCACTGCATAATTCTCTTACTGTCATGCCATCCGTAAGATGCTTTTCTGTGACTGGTGAGTACTCAACCAAGTCATTCTGAGAATAGTGTATGCGGCGACCGAGTTGCTCTTGCCCGGCGTCAACACGGGATAATACCGCGCCACATAGCAGAACTTTAAAAGTGCTCATCATTGGAAAACGTTCTTCGGGGCGAAAACTCTCAAGGATCTTACCGCTGTTGAGATCCAGTTCGATGTAACCCACTCGTGCACCCAACTGATCTTCAGCATCTTTTACTTTCACCAGCGTTTCTGGGTGAGCAAAAACAGGAAGGCAAAATGCCGCAAAAAAGGGAATAAGGGCGACACGGAAATGTTGAATACTCATACTCTTCCTTTTTCAATATTATTGAAGCATTTATCAGGGTTATTGTCTCATGAGCGGATACATATTTGAATGTATTTAGAAAAATAAACAAATAGGGGTTCCGCGCACATTTCCCCGAAAAGTGCCACCTGACGTCTAAGAAACCATTATTATCATGACATTAACCTATAAAAATAGGCGTATCACGAGGCCCTTTCGT
CTTCAAGGAATTAATTCTATATTCTAAAAACACAAATGATACTTCTAAAAAAAATCATGAATGGCATCAACTCTGAATCAAATCTTTGCAGATGCACCTACTTCTCATTTCCACTGTCACATCATTTTTCCAGATCTCGCTGCCTGTTATGTGGCCCACAAACCAAGACACGTTTTATGGCCATTAAAGCTGGCTGATCGTCGCCAAACACCAAATACATATCAATATGTACATTCGAGAAAGAAGCGATCAAAGAAGCGTCTTCGGGCGAGTAGGAGAATGCGGAGGAGAAGGAGAACGAGCTGATCTAGTATCTCTCCACAATCCAATGCCAACTGACCAACTGGCCATATTCGGAGCAATTTGAAGCCAATTTCCATCGCCTGGCGATCGCTCCATTCTTGGCTATATGTTTTTCACCGTTCCCGGGGCCATTTTCAAAGACTCGTCGGTAAGATAAGATTGTGTCACTCGCTGTCTCTCTTCATTTGTCGAAGAATGCTGAGGAATTTCGCGATGACGTCGGCGAGTATTTTGAAGAATGAGAATAATTTGTATTTATACGAAAATCAGTTAGTGGAATTTTCTACAAAAACATGTTATCTATAGATAATTTTGTTGCAAAATATGTTGACTATGACAAAGATTGTATGTATATACCTTTAATGTATTCTCATTTTCTTATGTATTTATAATGGCAATGATGATACTGATGATATTTTAAGATGATGCCAGACCACAGGCTGATTTCTGCGTCTTTTGCCGAACGCAGTGCATGTGCGGTTGTTGTTTTTTGGAATAGTTTCAATTTTCGGACTGTCCGCTTTGATTTCAGTTTCTTGGCTTATTCAAAAAGCAAAGTAAAGCCAAAAAAGCGAGATGGCAATACCAAATGCGGCAAAACGGTAGTGGAAGGAAAGGGGTGCGGGGCAGCGGAAGGAAGGGTGGGGCGGGGCGTGGCGGGGTCTGTGGCTGGGCGCGACGTCACCGACGTTGGAGCCACTCCTTTGACCATGTGTGCGTGTGTGTATTATTCGTGTCTCGCCACTCGCCGGTTGTTTTTTTCTTTTTATCTCGCTCTCTCTAGCGCCATCTCGTACGCATGCTCAACGCACCGCATGTTGCCGTGTCCTTTATGCGTCATTTTGGCTCGAAATAGGCAATTATTTAAACAAAGATTAGTCAACGAAAACGCTAAAATAAATAAGTCTACAATATGGTTACTTATTGCCATGTGTGTGCAGCCAACGATAGCAACAAAAGCAACAACACAGTGGCTTTCCCTCTTTCACTTTTTGTTTGCAAGCGCGTGCGAGCAAGACGGCACGACCGGCAAACGCAATTACGCTGACAAAGAGCAGACGAAGTTTTGGCCGAAAAACATCAAGGCGCCTGATACGAATGCATTTGCAATAACAATTGCGATATTTAATATTGTTTATGAAGCTGTTTGACTTCAAAACACACAAAAAAAAAAATAAAACAAATTATTTGAAAGAGAATTAGGAATCGGACAGCTTATCGTTACGGGCTAACAGCACACCGAGACGAAATAGCTTACCTGACGTCACAGCCTCTGGAAGAACTGCCGCCAAGCAGAGAGAGAGAGAAAAAGAGGGAGAGCAGCTTAGACCGCATGTGCTTGTGTGTGAGGCGTCTCTCTCTTCGTCTCCTGTTTGCGCAAACGCATAGACTGCACTGAGAAAATCGATTACCTATTTTTTATGAATGAATATTTGCACTATTACTATTCAAAACTATTAAGATAGCAATCACATTCAATAGCCAAATACTATACCACCTGAGCGATGCAACGAAATGATCAATTTGAGCAAAAATGCTGCATATTTAGGACGGCATCATTATAGAAATGCTTCTTGCTGTGTACTTTTCTCTCGTCTGGCAGCTGTTTCGCCGTTATTGTTAAAACCGGCTTAAGTTAGGTGTGTTTTCTACGACTAGTGATGCCCCTACTAGAAGATGTGTGTTGCACAAATGTCCCTGAATAACCAATTTGAAGTGCAGATAGCAGTAAACGTAAGCTAATATGAATATTATTTAACTGTAATGTTTTAATATCGCTGGACATTACTAATAAACCCACTATAAACACATGTACATATGTATGTTTTGGCATACAATGAGTAGTTGGGGAAAAAATGTGTAAAAGCACCGTGACCATCACAGCATAAAGATAACCAGCTGAAGTATCGAATATGAGTAACCCCCAAATTGAATCACATGCCGCAACTGATAGGACCCATGGAAGTACACTCTTCATGGCGATATACAAGACACACACAAGCACGAACACCCAGTTGCGGAGGAAATTCTCCGTAAATGAAAACCCAATCGGCGAACAATTCATACCCATATATGGTAAAAGTTTTGAACGCGACTTGAGAGCGGAGAGCATTGCGGCTGATAAGGTTTTAGCGCTAAGCGGGCTTTATAAAACGGGCTGCGGGACCAGTTTTCATATCACTACCGTTTGAGTTCTTGTGCTGTGTGGATACTCCTCCCGACACAAAGCCGCTCCATCAGCCAGCAGTCGTCTAATCCAGAGACcccggatcggggtacctactagtccagtgtggtggaattctgcagatatccagcacagtggcggccgcTCTAGAactagtggatcccccgggctgcaggaattcggcacgaggtgacttcctctgttctgtgctgtgtataaattgtttttaggacctaggctaggaattactggttgcacacactcagcgccacagctccaccgaccaccgcccttgcaaggacccaccATGAACTCCAAGGACAAGTCCAAGTTCAAGTTGTTCCTCAAATCGCTGCCGGCAGGTTACGTGGGCGAGCGGACCCTGCGGCCGGAGTTTGAGAGGGAACTGCGTCCGGAGCAGCCGGTGGCCCAGCGCTGCCGGATGCTGAAAGAGCTGGGCGACACGCAGCTGCACAACTTCAATCTGGACGAGTCCGCCATCACCATTCTGTTCAATCTCACCAACGATCTCATCGTGCCCAACAAGCCGGCAGAAACGCGGCAGATTGCCTTGAGCTTCTATAAACGGCTCATTCACACACAGTACAAGAACCTGACCATCATGCGGGAGAAGTTCTTCCTGGTCATCCAGAACCACGAAGCCCGCGAAGATCTGCGTCACCTTCTTGAGCTGCTCGACACCCTCACCGACAATGGGAAGGATATCACAAACTTCGAGGAGAAGATTGGCAAGTTTATGCTGCTCTGGATCCCAGCCATTACGGAGGCCAATCTGCTTACCCCCTACCTTGACATTCTGGTCAACCTGATCAAGTTCAATGCGGCGCATCTGGACAAAGACATCCTTGTGGGCATCGTGCAGAACGCTTGCGATCTGAGCTGTAGCGTTACCGTAAACGAAATCGGTCTGCAGTGCCTCACAATTCTGGAGATGGTCATCGGGTACACCATCTTCCCAAGCGAACCGCTGCCGCAGTGCATCACCACCTTGTGCCGGACTGTGAATCATGCTCCTTACTGCCCGTCTTCTTTCAAGATCATGAAGAACCTCTTGGGCACCCAGTTGGGATACCATTCCATGAAGATGATGTGCAGTATCCTCAACGATCGAGCTCTTTACGATGATGCCCACCTGTTGCGTGGGGCCGTGTTCCATCTAAACATGAACATCTTTGGCTCGAACATTATTTTCCAGGTTTCGCCTATGACCTATGCAACGAATGTGCTGACAGCCTTCCTGCGGGCACTAGACAGTCGTCAGGTGATCGTGAccttcgaagtcatcctcagcGTTCGCATGGTGATTACCAAGCGGCAGCTTTCAGAGATCATTTGGGATCTGATCTGCGATATAATGTCCTCGATCGTTAGCAATATCGAGTATTACGAAGAAGTCAATATAAACAAGGATCGTCTGCATCACCTGCAGATCAATTTCCACGAGAATATCGACTGCATCGAGAAGCTGTTGCAGCAGGATAGATCCCAGATTCTGGGCAATGTGGAGCGCATCTACGATCTCATCGAGCGAGTTGCCGACCGACGCCCAGAGGCTTCCGTTCTAGCCCTAATCGAGTACCGATCCCGACGGGTTACAGCAACGCGGCCAGATTGGCTTCAGGTGCTGGCCCAGTTCGTCCGGCGCTACtaccgcatgtccaacgttaaCGTGCGCATTAAGACGATCGAGGCTCTAGTTCAGATTATGGATCAAAACCGTGCCTGCTATGAAGAAGAAATCCTTAGTCGCGTGGTGCTGGTGCACCTTTCCCATATTCATCTGGAGTCGAGTGTGCAGGTGCGAGTGGCCGTGGCCCGGGCTCTTTCCAATTTTGCCACCCATTGCGACACGAAACGGTGCATGGATCTGCTAGATATCCTGGAGGCGCTCATCAATCGACCCTTTGAGCACACGCGGCATGGATCCTCATCTGGAGAGGGTACACTTGCAGAAGTGACCTTTGGATTGGTCAACAACGAATCCGAGATCTCGGATATTATCGCCGCAGTTGATGGTTTGGTCAAAGTGTTTGCCATCAAGCTGCATCGTCTGCCAGGGATCCATGCTCTTAAAATATTTAACATTCTAATGGACCACCTGGAACTGCACTACGAACGACCAAAGATTTTCGAGCACATAAGTGTTGTGCGCTATAAAATATTTGCTTGGTTGCTGAAGGCCCGGGCCAACGGCTCCTACCATATTGGTTATCCGGAGGGCAGCACCGAGGTGGTGAAATTTAGTCCATACCTGGGAATTGATTCACCACTGTTACCACAAGCCCACCCGACAGCGATATCCATACGACGAGTCTGCAAGCTGATAGTGAGGTGCCTGGAACACGACACCGATTATCAGGTGTTCCAGCTAGTCATCAGGGAACTGCCGAAGGTGCTGCAGAACAAGGCGCTGGTGCAGGGCAATGACATCGAGGAGCTGGCCAACACGCTCCTCAAGATCAATTTGGTTAGCAACAACAAGTTCAAGCGACCCACCGATGAGTTCCACGCCCTCGTTTTACCCGCCATAGCTTCATTGGTTATCTATCACGAAAGCCTGCAGCCGCAGCAGCACTACGGAATTATAACAGCTCTCAATTCGAGGGTACTTACTGGCATTGCCAGCGTCTGCATCAACACCATGACCATCCTTATACTAGAGATGCCGGAGGCGTTGATGCGAAAGCTGCCGGACGTACTGCTCCAAATGAGTAAGATGTCGGATACGAATGCGTTGGCCACTCCGGTTCTGGAATTTCTGTCGTTGCTCATACATCTGTCCAAGCATCTCTTCGCAAACTTCACTTCCATGCACAACATGTACGTGTTTGCCATCACGCTGCCGTACACCAAACCGCATCGCTACGATCACTATACAGTTTCCTTGGCCCACCATGTCATCGCTGGATGGTTCCTTAAGTGTAAGCTGGAGCTGCGCAGGAACTGCGTTAACTACATCAAAAGCTCGATTCAGTCGAATGCCAAGATGTTGTCCAGCGACATTGTGAACCTAAACTCCTTAAACGAGGATTCATCCAATCGCAAGCGGAGCACCAGTCTAACTGAGCGCGGTAGTCGCAACAATGCCAATGCCTGGAACGATCTGGAGATGCGACCGCAAATGAACAACGGTCTACGAAACTTCCACGCCGAACTGGCAGAGACGTGCTTTGATTTCTTGGCGCGACACACTTATTCGCCTTGTCCGTCGATGCCCAAGCGTCTTCCAGCGGCGGAATTCCTGCTGAAGGACGGCGTCTCGCAGACGTGGCTAGTGGGCAACAACCTGGTGACCATAACCACCTCCGGCTGTCCGTCGGCGCCCGCGCGCAGTGGGCTTTGCGAACGATGTGCCCAGCTAGGAAAGGCTCCCAGCATTAGTCTGAACTCGAAGAGCCTCAGTGATGCAGCTCCTCCACTTgCGCCAGAGCGGGAGAGACGATACACCAAGGTGAGCCTGCAGCACAGCAGCGGCAACGAGTCGGCTGGCAGCACGGAGCTCACCTCCTCCTCGTCGTCGAACTCAGCAGCCGCAGGTGGTCATCCACATCGACAGATTTCTAACAGTTCCACGGCATCGCTAGACGCGCTTTCCCGTCGGGGGTCTAATCCCGAAGCCTTGGGCAGTGCCTTGGGCGAGGGGGCACATACGGGGAGCAACACCTCGCTCCTGGGAAACTCTTTGTCCCAATCCTCCGTTAGCATGAGCCCTTCGTCGGGATCCGTGGTGCAGCAGCCGGTTTGCGTGCGCGCCTGCACTGGCTGGGCGGAGGTTTTGGTTCGTCGGCCCACGGGCAACGTATCGTGGATAACCCGCATTCAGAACCCCATCACCAACGATTGCTTTGGCCAGGAGTTGCCGTTCAATAATGTGGTGTCGCTGTTTCTTCCCACTGCCCATGGCGGAGTTTTTGGACCTGACAATACCATTCAGGCCCCACCACATACGTTGGCTGATCCCGAACCAGAACCAGAAGCAGAGGTTAATGCAGCAACAGCTCCTCAGGCAAGTGCATTGCGCAGCCGAATGGTTGCCAAGGCTCGTGCCCTTCAGCGACAGGAAGAAATTCACTCGGTCGGAGGAAATGGAAATGGCAACGGCAACGGTAATGGTAGTGGAAATGCTCCCAGCACTGGAGGTGCTGCTGCCATACCCATTCCGCGGGTCCCAGCATCTGGAAAACGCGGCAAGGAGGCAGCTCTTTgtggaagtgtgagtgatggTGAAGCCGACGATGACTCCCTGGCCTTTGAGGATGCTCAGAGTCGGGCACGAAATCCCGTCCGACGGGTAAATTCCAGCCCAGAGATGAGTTCCAGTTGGCGGCAGTCGTTCCTAACCAACAAACCCACGCCACTGTCCCAGGAGCCCGTGAAAACCACCGCCGATGAACCGCAATCGCTGTTGACTTTGAAGAAGAAGACTGTTCAGTACAGCACCAAGGATATGCGTGTGAGCTGTGAGGCCATTCCGGAGGAGATCGCCGGCTCCACACCACCGTCGCAGGCCGCAGCCTTGGCCCTGCAGCCGGAAAGCGGCACCCTACCTCCCAAGCAGCATTCGGCGGACGATGTCAGCAGTCATGTGGCTGGAGGAAGTAACTTGCAAGCAGGAGGGTCCACGCTAAAGCTGGGTAAACCACCGCTGTCGCCGGGGCAACCGCCTCTGCTGGGCACTGGTCGAGTGACCAGCTTTGGTGGCACCTCTGTTCCGCAGCCCGGTGCACTGGCAAAATCGAGCAGCAGTGGCAGCAATGGCACCAATGTTGGAGTGATAACCTCCGATTACGACAACGGCAACAATGGAAACGGAGACATGATGCGAGGTCGTTCGAAAACCATTTCGGTGGTGCGTGAAGTGAATAACGGAAATACGCGTCCGCCGCCGGCCAGCAGTTTCCGGAATTTCGGAGCCGCAAAGCCGCCCATCAACACCAAGCTGTGCATGAACCCGAGCTTTATTTTCATGCAACTGTACACCACTGGCCAGCTGGGAGTTACGGATGAGCCTCTAAAGGTGGGTCCGGAAAACAGCAGCGCTGTCTCCCTTATCGATTTAGTCCCACCGTTCGAGACGCACAAGATCGGCGTGCTGTACGTGGGCCAAGGGCAGTGCAACAACGAGGTGGAGATCTTGCGCAATTCGCACGGAAGTGCCAGGTATGTGGAGTTCCTGCGTAAcatcggcaccctggtcagtctgAAGGAAGCTGAGCAGAACAATCTATTTATAATGCTAGATAGAAACGGAGCAGATGGCAAGTTTGCCTATATCTGGAAGGATGACATACTGCAGGTTACGTTCCATGTCGCTACGCTGATGCCCACCAATCTGCAGGATGATCCCAACTGCAACGAGAAGAAGAGCCACATTGGCAATGACTTTGTGAAGATCATCTACAACGAAAgcggcgaggagtacaatctaaacacAATATCCGGCCAATTCAACTACGCCTGCGTGATCGTTGAGCCACTTGACCTGAACTCGAACAGGGTGTATGTGAAGGCGCGATCGGAGATTTCGAAATTCGTGTGCCACGCAGAATACCGGATAGTGTCCGATCGCAGTGCTCCTCTTTTGGCCCGACAGATGGCGCTGCACGccaatctcgcgtcgctagtctacCAAAGTGTGCAGAAAAAGCATCCGTACGCATCGAATTGGCTAGAACGATTGCGCAAACTAAAGCGCCTGCGATCGAAGCTAATTGAGGGCCTAAACAGCCAGCAAAAATCCGGAAGCGCCTCGAGcggcatcggaatcaacgcctcagccattggttcggatatggacgaccagcgaggtgacttcattaaatacacagagggcccgcggttcgaaggtaagcctatccctaaccctctcctcggtctcgattctacgcgtaccggtcatcatcaccatcaccattgagtttaaacccgctgatcagcctcgactgtgccttctaaggcctgagctcgcTGATCAGCCTCGATCGAGGATCCAGACATGATAAGATACATTGATGAGTTTGGACAAACCACAACTAGAATGCAGTGAAAAAAATGCTTTATTTGTGAAATTTGTGATGCTATTGCTTTATTTGTAACCATTATAAGCTGCAATAAACAAGTTAACAACAACAATTGCATTCATTTTATGTTTCAGGTTCAGGGGGAGGTGTGGGAGGTTTTTTAAAGCAAGTAAAACCTCTACAAATGTGGTATGGCTGATTATGATCAGTCGACCGATGCCCTTGAGAGCCTTCAACCCAGTCAGCTCCTTCCGGTGGGCGCGGGGCATGACTATCGTCGCCGCACTTATGACTGTCTTCTTTATCATGCAACTCGTAGGACAGGTGCCGGCAGCGCTCTGGGTCATTTTCGGCGAGGACCGCTTTCGCTGGAGCGCGACGATGATCGGCCTGTCGCTTGCGGTATTCGGAATCTTGCACGCCCTCGCTCAAGCCTTCGTCACTGGTCCCGCCACCAAACGTTTCGGCGAGAAGCAGGCCATTATCGCCGGCATGGCGGCCGACGCGCTGGGCTACGTCTTGCTGGCGTTCGCGACGCGAGGCTGGATGGCCTTCCCCATTATGATTCTTCTCGCTTCCGGCGGCATCGGGATGCCCGCGTTGCAGGCCATGCTGTCCAGGCAGGTAGATGACGACCATCAGGGACAGCTTCAAGGATCGCTCGCGGCTCTTACCAGCCAGCAAAAGGCCAGGAACCGTAAAAAGGCCGCGTTGCTGGCGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCGACGCTCAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCCTGGAAGCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCTTTCTCCCTTCGGGAAGCGTGGCGCTTTCTCAATGCTCACGCTGTAGGTATCTCAGTTCGGTGTAGGTCGTTCGCTCCAAGCTGGGCTGTGTGCACGAACCCCCCGTTCAGCCCGACCGCTGCGCCTTATCCGGTAACTATCGTCTTGAGTCCAACCCGGTAAGACACGACTTATCGCCACTGGCAGCAGCCACTGGTAACAGGATTAGCAGAGCGAGGTATGTAGGCGGTGCTACAGAGTTCTTGAAGTGGTGGCCTAACTACGGCTACACTAGAAGGACAGTATTTGGTATCTGCGCTCTGCTGAAGCCAGTTACCTTCGGAAAAAGAGTTGGTAGCTCTTGATCCGGCAAACAAACCACCGCTGGTAGCGGTGGTTTTTTTGTTTGCAAGCAGCAGATTACGCGCAGAAAAAAAGGATCTCAAGAAGATCCTTTGATCTTTTCTACGGGGTCTGACGCTCAGTGGAACGAAAACTCACGTTAAGGGATTTTGGTCATGAGATTATCAAAAAGGATCTTCACCTAGATCCTTTTAAATTAAAAATGAAGTTTTAAATCAATCTAAAGTATATATGAGTAAACTTGGTCTGACAGTTACCAATGCTTAATCAGTGAGGCACCTATCTCAGCGATCTGTCTATTTCGTTCATCCATAGTTGCCTGACTCCCCGTCGTGTAGATAACTACGATACGGGAGGGCTTACCATCTGGCCCCAGTGCTGCAATGATACCGCGAGACCCACGCTCACCGGCTCCAGATTTATCAGCAATAAACCAGCCAGCCGGAAGGGCCGAGCGCAGAAGTGGTCCTGCAACTTTATCCGCCTCCATCCAGTCTATTAATTGTTGCCGGGAAGCTAGAGTAAGTAGTTCGCCAGTTAATAGTTTGCGCAACGTTGTTGCCATTGCTGCAGGCATCGTGGTGTCACGCTCGTCGTTTGGTATGGCTTCATTCAGCTCCGGTTCCCAACGATCAAGGCGAGTTACATGATCCCCCATGTTGTGCAAAAAAGCGGTTAGCTCCTTCGGTCCTCCGATCGTTGTCAGAAGTAAGTTGGCCGCAGTGTTATCACTCATGGTTATGGCAGCACTGCATAATTCTCTTACTGTCATGCCATCCGTAAGATGCTTTTCTGTGACTGGTGAGTACTCAACCAAGTCATTCTGAGAATAGTGTATGCGGCGACCGAGTTGCTCTTGCCCGGCGTCAACACGGGATAATACCGCGCCACATAGCAGAACTTTAAAAGTGCTCATCATTGGAAAACGTTCTTCGGGGCGAAAACTCTCAAGGATCTTACCGCTGTTGAGATCCAGTTCGATGTAACCCACTCGTGCACCCAACTGATCTTCAGCATCTTTTACTTTCACCAGCGTTTCTGGGTGAGCAAAAACAGGAAGGCAAAATGCCGCAAAAAAGGGAATAAGGGCGACACGGAAATGTTGAATACTCATACTCTTCCTTTTTCAATATTATTGAAGCATTTATCAGGGTTATTGTCTCATGAGCGGATACATATTTGAATGTATTTAGAAAAATAAACAAATAGGGGTTCCGCGCACATTTCCCCGAAAAGTGCCACCTGACGTCTAAGAAACCATTATTATCATGACATTAACCTATAAAAATAGGCGTATCACGAGGCCCTTTCGT
