EPR Plasmid #353: pRSFDuet-1 6xHis-HRV3C-Naa35 | Naa38ΔC11 | Naa30ΔC17
Plasmid Details
| Purpose | Coexpression of the three NatC subunits from S. cerevisiae in E. coli for affinity purification via a cleavable His-tag on Naa35 |
| Depositing Lab |
Oliver Daumke Max-Delbrueck-Center for Molecular Medicine |
| Depositing Scientist | Oliver Daumke |
| Antibiotic Resistance | Kanamycin |
| Bacterial Growth Temp | 37 |
| Copy Number | High |
| Selectable Marker | |
| Reference |
Divergent architecture of the heterotrimeric NatC complex explains N-terminal acetylation of cognate substrates. Grunwald S et al. Nat Commun 2020 PubMed DOI See other plasmids from this publication |
| MTA and Terms |
MTA (PDF download) |
How to Cite This Reagent
IN THE MATERIALS & METHODS:
pRSFDuet-1 6xHis-HRV3C-Naa35 | Naa38ΔC11 | Naa30ΔC17 was a gift from Oliver Daumke (European Plasmid Repository plasmid #353).
pRSFDuet-1 6xHis-HRV3C-Naa35 | Naa38ΔC11 | Naa30ΔC17 was a gift from Oliver Daumke (European Plasmid Repository plasmid #353).
IN THE REFERENCES:
Grunwald S et al.. Divergent architecture of the heterotrimeric NatC complex explains N-terminal acetylation of cognate substrates.. Nat Commun 2020 doi:10.1038/s41467-020-19321-8 PubMed 33139728
Grunwald S et al.. Divergent architecture of the heterotrimeric NatC complex explains N-terminal acetylation of cognate substrates.. Nat Commun 2020 doi:10.1038/s41467-020-19321-8 PubMed 33139728
Sequences
Provided by depositor
download sequence
GGGGAATTGTGAGCGGATAACAATTCCCCTGTAGAAATAATTTTGTTTAACTTTAATAAGGAGATATACCATGGGCAGCA GCCATCACCATCATCACCACAGCCAGGATCCGCTGGAAGTTCTGTTCCAGGGGCCCATGGAAGTAGACAGTATATTAGGT TCCTTGAGCATAACTGATGATTTTGACCAATTGGTTGACGTCACAAGTCTGTTTGATGAATTGTGTTCAAAACTAAAACC AGAGGCTATCGTGAAAGATCCCAGGTTTGATTTGTTCGAGGGTACACATTCTTTAGAGGTCAACAACTCCAAATTAGACT CCAGTCTGATAGAATTGACAGCAGAAGAAATAGAATTTGATGTGAACGTGGCCTACGATCCACCGTTGGCAAGTGTAGCA GCCATAGCGGATAGATTACTTAGATGTGTTATCTCCTGGCTGAATGACTACCAGACTTTACCCACTACTGTATTAAGCTG CAGGTATACAGAAAGCTTATTATCGTCATTAGTTAAGGGAACAACTGCCGGTTCGTCCTGGTGTACAGGGAATATTTTGT ATGATAAAGTACTAGGAAGTTGCATACTTGGCGTTTGTTATTTAACAAAATTTGTGCAGAAACTCTTGAGTGCTGGTATT GTTTTTGAAGAGGAGGATTTAAATTTTAATAACATGGGGTTTAATACATTCGATAATTTGCCGGGGCAAGATGTTGTTAT CAATTCTCTCACCGAGAGCCTTCAGATATTAGAGGCATATTCTGATGATAGCTTACATTTAACTATGCTGAAGCATATAC TAAAAATTATCATTTGCTTAGTCCACTTAGAAGACCATCTGACAGACTATTCCACAAAGACAAGCCACCTGGATGAGCTA ATTGAAAACGCTAACTCGGTTAATGGCATATTTCCACAATTACAGCTGTCTCCACCAAAGGGTGCGTTCTCAACATATAT ACAAAAGCACAGGTCTAATCAATTCCCTCCCAGGAAGATTACGAAGTTACCGACGGATTACAGCGGCTTTATTACACTGG CAAATGATGTTAAAACAATACTCCTTGTAGATAAAGCCGAGTCTGCGTTGGAGACCTACCAGTTTGCCAAATTCTTCAAC AAGTTGGAACAAAGGCATGTCATTGCAAGGATCTTATTCCCCTTATTTTTCATTAGAGACGACAGAACAGTTTTGGGTAA ATTTTCATATACCCAATTTTATTTGCTACATGTCAAGGAGTTTTCAGCACAAACTCCATCTGAATTTGAATCCTCTATTG GTAATGAACTTATCCAAGAAAGCTCTAATATGCTTTTAGAATGGTACCAAAACTGCTCCCAAAACACATGTAGATATAGA CAAGGTTTTAACAGGCAACTTATTCTATGGGATTCTCTTCAAGCCCAGTTTGAAAGTGTCAATTCACAAGTTTACTGTTC GTGGACATATTTTATGAAACTATCAAGTATGATCGAATTCTCTTTAAAGGGGTTTGATTTGGATATTTACAAACCCTTTG AAGCATACTCTATGTTTTGGTATGTTTATTATTTAAGTCACCACTTAGAAACCTTTTTGAAAGATAGTCAAAATGATATT GAATCAAATATTAATGCCATTCATTCCATGAACAAAAAACTTAAAAAATTGAAAGCTGGTGAAAAGAAGGACCAATTGAG GCTAAAGTACAGGTTTGCTATGGATAATGAAATGGAACAATTACAAGCTACCAAACAATTTTTAAACTATCTTTTGAAGG AAATTAACATTACCAAATCTTTATGTCTAATAGAGGTTTTCCAATTTGCAATTTTAAAGTCATTTGGTTTAATTGACAAC AAGAATAGTACTCCGTCAAAATTCTCTAATGAACGGTTAATCCATAATTTGAGATTCAAGCCATTTAATTCAATTGGTGT TCCTGAGTTACCAGAATATGAAGTATTTCAACAAACTTTAAAAGATTTTGTCATTGAAGAAAAAGGAGCTGCATTTGATA TAAAGTTAGAAAGGGCCACTAACTTTATCGAAACTGAAGTGCGCAATGTCGTTAGTTCTATTGATGAGATAATGCAAGGA ATCAAGGGAGGCGATAATAACGGCGTTCTAGTAACAGGAACAAGACTGGTTCAAGAGCTCTCTCTGGAGTACTATTGTAA ACTAAAACATACTTCTAAAGCATTATCAGTCAACTCAAAAGTTATCGTCAACACTTTGAAAAAGAACATTAAAAACAAGG ACTCCCACGAATACAAGGTTGAATTAGTTCATACAACAGAAGGATGGAACTATTTCCCCATACAAACATTGAGAATAAAG CAAGACCGCTATAAATAAGCGGCCGCATAATGCTTAAGTCGAACAGAAAGTAATCGTATTGTACACGGCCGCATAATCGA AATTAATACGACTCACTATAGGGGAATTGTGAGCGGATAACAATTCCCCATCTTAGTATATTAGTTAAGTATAAGAAGGA GATATACATATGGACATCTTGAAACTGTCAGATTTTATTGGAAATACTTTAATAGTTTCCCTTACAGAAGATCGTATTTT AGTTGGAAGCTTGGTTGCTGTAGATGCCCAAATGAATTTGCTATTAGATCATGTTGAGGAACGTATGGGCTCCAGTAGTA GAATGATGGGCCTAGTCAGCGTCCCTAGGCGTTCCGTTAAGACCATAATGATTGATAAGCCTGTTCTGCAGGAGCTTACT TAGCAATTGGATATCGGCCGGCCACGCGATCGAAGGAGATATACCATGGAAATAGTGTACAAGCCATTGGACATACGTAA CGAAGAGCAGTTTGCTAGTATTAAAAAACTAATAGATGCGGATCTATCAGAGCCGTACTCGATATACGTATATAGGTATT TCTTGAACCAATGGCCCGAGTTAACGTACATTGCCGTTGACAACAAGAGTGGAACGCCTAACATACCCATTGGTTGCATA GTGTGTAAGATGGACCCGCATAGAAACGTGAGACTGAGAGGATATATAGGAATGCTAGCCGTAGAGTCCACATACCGCGG GCATGGCATAGCAAAGAAACTAGTGGAAATTGCCATAGATAAAATGCAAAGGGAACATTGCGATGAGATCATGTTAGAAA CAGAGGTGGAAAATTCGGCGGCTCTAAACTTATATGAAGGAATGGGTTTCATCAGAATGAAACGGATGTTCCGCTACTAC TTGAATGAAGGGGACGCCTTTAAACTGATATTGCCGTTGACTTAATGACTCGAGTCTGGTAAAGAAACCGCTGCTGCGAA ATTTGAACGCCAGCACATGGACTCGTCTACTAGCGCAGCTTAATTAACCTAGGCTGCTGCCACCGCTGAGCAATAACTAG CATAACCCCTTGGGGCCTCTAAACGGGTCTTGAGGGGTTTTTTGCTGAAACCTCAGGCATTTGAGAAGCACACGGTCACA CTGCTTCCGGTAGTCAATAAACCGGTAAACCAGCAATAGACATAAGCGGCTATTTAACGACCCTGCCCTGAACCGACGAC AAGCTGACGACCGGGTCTCCGCAAGTGGCACTTTTCGGGGAAATGTGCGCGGAACCCCTATTTGTTTATTTTTCTAAATA CATTCAAATATGTATCCGCTCATGAATTAATTCTTAGAAAAACTCATCGAGCATCAAATGAAACTGCAATTTATTCATAT CAGGATTATCAATACCATATTTTTGAAAAAGCCGTTTCTGTAATGAAGGAGAAAACTCACCGAGGCAGTTCCATAGGATG GCAAGATCCTGGTATCGGTCTGCGATTCCGACTCGTCCAACATCAATACAACCTATTAATTTCCCCTCGTCAAAAATAAG GTTATCAAGTGAGAAATCACCATGAGTGACGACTGAATCCGGTGAGAATGGCAAAAGTTTATGCATTTCTTTCCAGACTT GTTCAACAGGCCAGCCATTACGCTCGTCATCAAAATCACTCGCATCAACCAAACCGTTATTCATTCGTGATTGCGCCTGA GCGAGACGAAATACGCGGTCGCTGTTAAAAGGACAATTACAAACAGGAATCGAATGCAACCGGCGCAGGAACACTGCCAG CGCATCAACAATATTTTCACCTGAATCAGGATATTCTTCTAATACCTGGAATGCTGTTTTCCCGGGGATCGCAGTGGTGA GTAACCATGCATCATCAGGAGTACGGATAAAATGCTTGATGGTCGGAAGAGGCATAAATTCCGTCAGCCAGTTTAGTCTG ACCATCTCATCTGTAACATCATTGGCAACGCTACCTTTGCCATGTTTCAGAAACAACTCTGGCGCATCGGGCTTCCCATA CAATCGATAGATTGTCGCACCTGATTGCCCGACATTATCGCGAGCCCATTTATACCCATATAAATCAGCATCCATGTTGG AATTTAATCGCGGCCTAGAGCAAGACGTTTCCCGTTGAATATGGCTCATACTCTTCCTTTTTCAATATTATTGAAGCATT TATCAGGGTTATTGTCTCATGAGCGGATACATATTTGAATGTATTTAGAAAAATAAACAAATAGGCATGCAGCGCTCTTC CGCTTCCTCGCTCACTGACTCGCTACGCTCGGTCGTTCGACTGCGGCGAGCGGTGTCAGCTCACTCAAAAGCGGTAATAC GGTTATCCACAGAATCAGGGGATAAAGCCGGAAAGAACATGTGAGCAAAAAGCAAAGCACCGGAAGAAGCCAACGCCGCA GGCGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCGACGCTCAAGCCAGAGGTGGCGAAACCCGACAG GACTATAAAGATACCAGGCGTTTCCCCCTGGAAGCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATAC CTGTCCGCCTTTCTCCCTTCGGGAAGCGTGGCGCTTTCTCATAGCTCACGCTGTTGGTATCTCAGTTCGGTGTAGGTCGT TCGCTCCAAGCTGGGCTGTGTGCACGAACCCCCCGTTCAGCCCGACCGCTGCGCCTTATCCGGTAACTATCGTCTTGAGT CCAACCCGGTAAGACACGACTTATCGCCACTGGCAGCAGCCATTGGTAACTGATTTAGAGGACTTTGTCTTGAAGTTATG CACCTGTTAAGGCTAAACTGAAAGAACAGATTTTGGTGAGTGCGGTCCTCCAACCCACTTACCTTGGTTCAAAGAGTTGG TAGCTCAGCGAACCTTGAGAAAACCACCGTTGGTAGCGGTGGTTTTTCTTTATTTATGAGATGATGAATCAATCGGTCTA TCAAGTCAACGAACAGCTATTCCGTTACTCTAGATTTCAGTGCAATTTATCTCTTCAAATGTAGCACCTGAAGTCAGCCC CATACGATATAAGTTGTAATTCTCATGTTAGTCATGCCCCGCGCCCACCGGAAGGAGCTGACTGGGTTGAAGGCTCTCAA GGGCATCGGTCGAGATCCCGGTGCCTAATGAGTGAGCTAACTTACATTAATTGCGTTGCGCTCACTGCCCGCTTTCCAGT CGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGCGTATTGGGCGCCAGGGT GGTTTTTCTTTTCACCAGTGAGACGGGCAACAGCTGATTGCCCTTCACCGCCTGGCCCTGAGAGAGTTGCAGCAAGCGGT CCACGCTGGTTTGCCCCAGCAGGCGAAAATCCTGTTTGATGGTGGTTAACGGCGGGATATAACATGAGCTGTCTTCGGTA TCGTCGTATCCCACTACCGAGATGTCCGCACCAACGCGCAGCCCGGACTCGGTAATGGCGCGCATTGCGCCCAGCGCCAT CTGATCGTTGGCAACCAGCATCGCAGTGGGAACGATGCCCTCATTCAGCATTTGCATGGTTTGTTGAAAACCGGACATGG CACTCCAGTCGCCTTCCCGTTCCGCTATCGGCTGAATTTGATTGCGAGTGAGATATTTATGCCAGCCAGCCAGACGCAGA CGCGCCGAGACAGAACTTAATGGGCCCGCTAACAGCGCGATTTGCTGGTGACCCAATGCGACCAGATGCTCCACGCCCAG TCGCGTACCGTCTTCATGGGAGAAAATAATACTGTTGATGGGTGTCTGGTCAGAGACATCAAGAAATAACGCCGGAACAT TAGTGCAGGCAGCTTCCACAGCAATGGCATCCTGGTCATCCAGCGGATAGTTAATGATCAGCCCACTGACGCGTTGCGCG AGAAGATTGTGCACCGCCGCTTTACAGGCTTCGACGCCGCTTCGTTCTACCATCGACACCACCACGCTGGCACCCAGTTG ATCGGCGCGAGATTTAATCGCCGCGACAATTTGCGACGGCGCGTGCAGGGCCAGACTGGAGGTGGCAACGCCAATCAGCA ACGACTGTTTGCCCGCCAGTTGTTGTGCCACGCGGTTGGGAATGTAATTCAGCTCCGCCATCGCCGCTTCCACTTTTTCC CGCGTTTTCGCAGAAACGTGGCTGGCCTGGTTCACCACGCGGGAAACGGTCTGATAAGAGACACCGGCATACTCTGCGAC ATCGTATAACGTTACTGGTTTCACATTCACCACCCTGAATTGACTCTCTTCCGGGCGCTATCATGCCATACCGCGAAAGG TTTTGCGCCATTCGATGGTGTCCGGGATCTCGACGCTCTCCCTTATGCGACTCCTGCATTAGGAAATTAATACGACTCAC TATA
GGGGAATTGTGAGCGGATAACAATTCCCCTGTAGAAATAATTTTGTTTAACTTTAATAAGGAGATATACCATGGGCAGCA GCCATCACCATCATCACCACAGCCAGGATCCGCTGGAAGTTCTGTTCCAGGGGCCCATGGAAGTAGACAGTATATTAGGT TCCTTGAGCATAACTGATGATTTTGACCAATTGGTTGACGTCACAAGTCTGTTTGATGAATTGTGTTCAAAACTAAAACC AGAGGCTATCGTGAAAGATCCCAGGTTTGATTTGTTCGAGGGTACACATTCTTTAGAGGTCAACAACTCCAAATTAGACT CCAGTCTGATAGAATTGACAGCAGAAGAAATAGAATTTGATGTGAACGTGGCCTACGATCCACCGTTGGCAAGTGTAGCA GCCATAGCGGATAGATTACTTAGATGTGTTATCTCCTGGCTGAATGACTACCAGACTTTACCCACTACTGTATTAAGCTG CAGGTATACAGAAAGCTTATTATCGTCATTAGTTAAGGGAACAACTGCCGGTTCGTCCTGGTGTACAGGGAATATTTTGT ATGATAAAGTACTAGGAAGTTGCATACTTGGCGTTTGTTATTTAACAAAATTTGTGCAGAAACTCTTGAGTGCTGGTATT GTTTTTGAAGAGGAGGATTTAAATTTTAATAACATGGGGTTTAATACATTCGATAATTTGCCGGGGCAAGATGTTGTTAT CAATTCTCTCACCGAGAGCCTTCAGATATTAGAGGCATATTCTGATGATAGCTTACATTTAACTATGCTGAAGCATATAC TAAAAATTATCATTTGCTTAGTCCACTTAGAAGACCATCTGACAGACTATTCCACAAAGACAAGCCACCTGGATGAGCTA ATTGAAAACGCTAACTCGGTTAATGGCATATTTCCACAATTACAGCTGTCTCCACCAAAGGGTGCGTTCTCAACATATAT ACAAAAGCACAGGTCTAATCAATTCCCTCCCAGGAAGATTACGAAGTTACCGACGGATTACAGCGGCTTTATTACACTGG CAAATGATGTTAAAACAATACTCCTTGTAGATAAAGCCGAGTCTGCGTTGGAGACCTACCAGTTTGCCAAATTCTTCAAC AAGTTGGAACAAAGGCATGTCATTGCAAGGATCTTATTCCCCTTATTTTTCATTAGAGACGACAGAACAGTTTTGGGTAA ATTTTCATATACCCAATTTTATTTGCTACATGTCAAGGAGTTTTCAGCACAAACTCCATCTGAATTTGAATCCTCTATTG GTAATGAACTTATCCAAGAAAGCTCTAATATGCTTTTAGAATGGTACCAAAACTGCTCCCAAAACACATGTAGATATAGA CAAGGTTTTAACAGGCAACTTATTCTATGGGATTCTCTTCAAGCCCAGTTTGAAAGTGTCAATTCACAAGTTTACTGTTC GTGGACATATTTTATGAAACTATCAAGTATGATCGAATTCTCTTTAAAGGGGTTTGATTTGGATATTTACAAACCCTTTG AAGCATACTCTATGTTTTGGTATGTTTATTATTTAAGTCACCACTTAGAAACCTTTTTGAAAGATAGTCAAAATGATATT GAATCAAATATTAATGCCATTCATTCCATGAACAAAAAACTTAAAAAATTGAAAGCTGGTGAAAAGAAGGACCAATTGAG GCTAAAGTACAGGTTTGCTATGGATAATGAAATGGAACAATTACAAGCTACCAAACAATTTTTAAACTATCTTTTGAAGG AAATTAACATTACCAAATCTTTATGTCTAATAGAGGTTTTCCAATTTGCAATTTTAAAGTCATTTGGTTTAATTGACAAC AAGAATAGTACTCCGTCAAAATTCTCTAATGAACGGTTAATCCATAATTTGAGATTCAAGCCATTTAATTCAATTGGTGT TCCTGAGTTACCAGAATATGAAGTATTTCAACAAACTTTAAAAGATTTTGTCATTGAAGAAAAAGGAGCTGCATTTGATA TAAAGTTAGAAAGGGCCACTAACTTTATCGAAACTGAAGTGCGCAATGTCGTTAGTTCTATTGATGAGATAATGCAAGGA ATCAAGGGAGGCGATAATAACGGCGTTCTAGTAACAGGAACAAGACTGGTTCAAGAGCTCTCTCTGGAGTACTATTGTAA ACTAAAACATACTTCTAAAGCATTATCAGTCAACTCAAAAGTTATCGTCAACACTTTGAAAAAGAACATTAAAAACAAGG ACTCCCACGAATACAAGGTTGAATTAGTTCATACAACAGAAGGATGGAACTATTTCCCCATACAAACATTGAGAATAAAG CAAGACCGCTATAAATAAGCGGCCGCATAATGCTTAAGTCGAACAGAAAGTAATCGTATTGTACACGGCCGCATAATCGA AATTAATACGACTCACTATAGGGGAATTGTGAGCGGATAACAATTCCCCATCTTAGTATATTAGTTAAGTATAAGAAGGA GATATACATATGGACATCTTGAAACTGTCAGATTTTATTGGAAATACTTTAATAGTTTCCCTTACAGAAGATCGTATTTT AGTTGGAAGCTTGGTTGCTGTAGATGCCCAAATGAATTTGCTATTAGATCATGTTGAGGAACGTATGGGCTCCAGTAGTA GAATGATGGGCCTAGTCAGCGTCCCTAGGCGTTCCGTTAAGACCATAATGATTGATAAGCCTGTTCTGCAGGAGCTTACT TAGCAATTGGATATCGGCCGGCCACGCGATCGAAGGAGATATACCATGGAAATAGTGTACAAGCCATTGGACATACGTAA CGAAGAGCAGTTTGCTAGTATTAAAAAACTAATAGATGCGGATCTATCAGAGCCGTACTCGATATACGTATATAGGTATT TCTTGAACCAATGGCCCGAGTTAACGTACATTGCCGTTGACAACAAGAGTGGAACGCCTAACATACCCATTGGTTGCATA GTGTGTAAGATGGACCCGCATAGAAACGTGAGACTGAGAGGATATATAGGAATGCTAGCCGTAGAGTCCACATACCGCGG GCATGGCATAGCAAAGAAACTAGTGGAAATTGCCATAGATAAAATGCAAAGGGAACATTGCGATGAGATCATGTTAGAAA CAGAGGTGGAAAATTCGGCGGCTCTAAACTTATATGAAGGAATGGGTTTCATCAGAATGAAACGGATGTTCCGCTACTAC TTGAATGAAGGGGACGCCTTTAAACTGATATTGCCGTTGACTTAATGACTCGAGTCTGGTAAAGAAACCGCTGCTGCGAA ATTTGAACGCCAGCACATGGACTCGTCTACTAGCGCAGCTTAATTAACCTAGGCTGCTGCCACCGCTGAGCAATAACTAG CATAACCCCTTGGGGCCTCTAAACGGGTCTTGAGGGGTTTTTTGCTGAAACCTCAGGCATTTGAGAAGCACACGGTCACA CTGCTTCCGGTAGTCAATAAACCGGTAAACCAGCAATAGACATAAGCGGCTATTTAACGACCCTGCCCTGAACCGACGAC AAGCTGACGACCGGGTCTCCGCAAGTGGCACTTTTCGGGGAAATGTGCGCGGAACCCCTATTTGTTTATTTTTCTAAATA CATTCAAATATGTATCCGCTCATGAATTAATTCTTAGAAAAACTCATCGAGCATCAAATGAAACTGCAATTTATTCATAT CAGGATTATCAATACCATATTTTTGAAAAAGCCGTTTCTGTAATGAAGGAGAAAACTCACCGAGGCAGTTCCATAGGATG GCAAGATCCTGGTATCGGTCTGCGATTCCGACTCGTCCAACATCAATACAACCTATTAATTTCCCCTCGTCAAAAATAAG GTTATCAAGTGAGAAATCACCATGAGTGACGACTGAATCCGGTGAGAATGGCAAAAGTTTATGCATTTCTTTCCAGACTT GTTCAACAGGCCAGCCATTACGCTCGTCATCAAAATCACTCGCATCAACCAAACCGTTATTCATTCGTGATTGCGCCTGA GCGAGACGAAATACGCGGTCGCTGTTAAAAGGACAATTACAAACAGGAATCGAATGCAACCGGCGCAGGAACACTGCCAG CGCATCAACAATATTTTCACCTGAATCAGGATATTCTTCTAATACCTGGAATGCTGTTTTCCCGGGGATCGCAGTGGTGA GTAACCATGCATCATCAGGAGTACGGATAAAATGCTTGATGGTCGGAAGAGGCATAAATTCCGTCAGCCAGTTTAGTCTG ACCATCTCATCTGTAACATCATTGGCAACGCTACCTTTGCCATGTTTCAGAAACAACTCTGGCGCATCGGGCTTCCCATA CAATCGATAGATTGTCGCACCTGATTGCCCGACATTATCGCGAGCCCATTTATACCCATATAAATCAGCATCCATGTTGG AATTTAATCGCGGCCTAGAGCAAGACGTTTCCCGTTGAATATGGCTCATACTCTTCCTTTTTCAATATTATTGAAGCATT TATCAGGGTTATTGTCTCATGAGCGGATACATATTTGAATGTATTTAGAAAAATAAACAAATAGGCATGCAGCGCTCTTC CGCTTCCTCGCTCACTGACTCGCTACGCTCGGTCGTTCGACTGCGGCGAGCGGTGTCAGCTCACTCAAAAGCGGTAATAC GGTTATCCACAGAATCAGGGGATAAAGCCGGAAAGAACATGTGAGCAAAAAGCAAAGCACCGGAAGAAGCCAACGCCGCA GGCGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCGACGCTCAAGCCAGAGGTGGCGAAACCCGACAG GACTATAAAGATACCAGGCGTTTCCCCCTGGAAGCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATAC CTGTCCGCCTTTCTCCCTTCGGGAAGCGTGGCGCTTTCTCATAGCTCACGCTGTTGGTATCTCAGTTCGGTGTAGGTCGT TCGCTCCAAGCTGGGCTGTGTGCACGAACCCCCCGTTCAGCCCGACCGCTGCGCCTTATCCGGTAACTATCGTCTTGAGT CCAACCCGGTAAGACACGACTTATCGCCACTGGCAGCAGCCATTGGTAACTGATTTAGAGGACTTTGTCTTGAAGTTATG CACCTGTTAAGGCTAAACTGAAAGAACAGATTTTGGTGAGTGCGGTCCTCCAACCCACTTACCTTGGTTCAAAGAGTTGG TAGCTCAGCGAACCTTGAGAAAACCACCGTTGGTAGCGGTGGTTTTTCTTTATTTATGAGATGATGAATCAATCGGTCTA TCAAGTCAACGAACAGCTATTCCGTTACTCTAGATTTCAGTGCAATTTATCTCTTCAAATGTAGCACCTGAAGTCAGCCC CATACGATATAAGTTGTAATTCTCATGTTAGTCATGCCCCGCGCCCACCGGAAGGAGCTGACTGGGTTGAAGGCTCTCAA GGGCATCGGTCGAGATCCCGGTGCCTAATGAGTGAGCTAACTTACATTAATTGCGTTGCGCTCACTGCCCGCTTTCCAGT CGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGCGTATTGGGCGCCAGGGT GGTTTTTCTTTTCACCAGTGAGACGGGCAACAGCTGATTGCCCTTCACCGCCTGGCCCTGAGAGAGTTGCAGCAAGCGGT CCACGCTGGTTTGCCCCAGCAGGCGAAAATCCTGTTTGATGGTGGTTAACGGCGGGATATAACATGAGCTGTCTTCGGTA TCGTCGTATCCCACTACCGAGATGTCCGCACCAACGCGCAGCCCGGACTCGGTAATGGCGCGCATTGCGCCCAGCGCCAT CTGATCGTTGGCAACCAGCATCGCAGTGGGAACGATGCCCTCATTCAGCATTTGCATGGTTTGTTGAAAACCGGACATGG CACTCCAGTCGCCTTCCCGTTCCGCTATCGGCTGAATTTGATTGCGAGTGAGATATTTATGCCAGCCAGCCAGACGCAGA CGCGCCGAGACAGAACTTAATGGGCCCGCTAACAGCGCGATTTGCTGGTGACCCAATGCGACCAGATGCTCCACGCCCAG TCGCGTACCGTCTTCATGGGAGAAAATAATACTGTTGATGGGTGTCTGGTCAGAGACATCAAGAAATAACGCCGGAACAT TAGTGCAGGCAGCTTCCACAGCAATGGCATCCTGGTCATCCAGCGGATAGTTAATGATCAGCCCACTGACGCGTTGCGCG AGAAGATTGTGCACCGCCGCTTTACAGGCTTCGACGCCGCTTCGTTCTACCATCGACACCACCACGCTGGCACCCAGTTG ATCGGCGCGAGATTTAATCGCCGCGACAATTTGCGACGGCGCGTGCAGGGCCAGACTGGAGGTGGCAACGCCAATCAGCA ACGACTGTTTGCCCGCCAGTTGTTGTGCCACGCGGTTGGGAATGTAATTCAGCTCCGCCATCGCCGCTTCCACTTTTTCC CGCGTTTTCGCAGAAACGTGGCTGGCCTGGTTCACCACGCGGGAAACGGTCTGATAAGAGACACCGGCATACTCTGCGAC ATCGTATAACGTTACTGGTTTCACATTCACCACCCTGAATTGACTCTCTTCCGGGCGCTATCATGCCATACCGCGAAAGG TTTTGCGCCATTCGATGGTGTCCGGGATCTCGACGCTCTCCCTTATGCGACTCCTGCATTAGGAAATTAATACGACTCAC TATA
