EPR Plasmid #420: ol_Il-1:GFP-T2A-Il-1-HA
Plasmid Details
| Purpose | Il-1 Reporter: Medaka interleukin-1 fused N-terminally to GFP via T2A and tagged with an HA tag. Il-1 is expressed under its own promoter (ca. 2 kb). The plasmid has I-SceI sites for meganuclease insertion |
| Depositing Lab |
Maria Leptin European Molecular Biology Laboratory (EMBL) |
| Depositing Scientist | Eva Hasel de Carvalho |
| Antibiotic Resistance | Ampicillin |
| Bacterial Growth Temp | 37 |
| Copy Number | High |
| Selectable Marker | |
| Reference |
Revisiting the origin of interleukin 1 in anamniotes and sub-functionalization of interleukin 1 in amniotes. Hasel de Carvalho E et al. Open Biol 2022 PubMed DOI See other plasmids from this publication |
| MTA and Terms |
MTA (PDF download) |
How to Cite This Reagent
IN THE MATERIALS & METHODS:
ol_Il-1:GFP-T2A-Il-1-HA was a gift from Maria Leptin (European Plasmid Repository plasmid #420).
ol_Il-1:GFP-T2A-Il-1-HA was a gift from Maria Leptin (European Plasmid Repository plasmid #420).
IN THE REFERENCES:
Hasel de Carvalho E et al.. Revisiting the origin of interleukin 1 in anamniotes and sub-functionalization of interleukin 1 in amniotes.. Open Biol 2022 doi:10.1098/rsob.220049 PubMed 35975650
Hasel de Carvalho E et al.. Revisiting the origin of interleukin 1 in anamniotes and sub-functionalization of interleukin 1 in amniotes.. Open Biol 2022 doi:10.1098/rsob.220049 PubMed 35975650
Sequences
Provided by depositor
download sequence
GTAAAACGACGGCCAGTGAGCGCGTAGGGATAACAGGGTAATGCGCGCGTAATACGACTCACTATANGGCGAATTGGGTACCGGGCCCCCCCTCGAGGAGGAGGAGTGGAAGTTCCAGAGCGGAGGAAGCAGAAATCATTTATGATCTGTAGTCAGCTGACTAATGTGTGTGTGGGGGAGGGGGGTCTTCTTTTACTACTTTAAACTCTGTCAGCAGTTTTTTCTTCTAGATTTGCTTTTCTATCTGCTTGTAGTTTTTCCTCATTTCTGAATGTGTTCTCCATCTTTGAATCTTCCTTTGTCCTAACAAAATTCTCTGAGGGCCTGAAAATAAAACCCAGTTGGAACTAAAAGCCACCATGATGACACTCCCACCGCCTGCACCGGAACAGGGAGCGACACGCTGAAAGGCCGGGAGCAGAGCCGGAAGTGAGCCGCTTTTGTCTCGGGTTCAATTCCAAAGGTGAGAAATTCCACAGCAGCCCCTCCCAGACGCACCGGGGACTTTTCTGGCTGAAACGGATCGGGGAGGGAATCAGGGCGTGTGCTGCTCAGAGGCCGATGCTCACCTGCACAGCTTTTCCTGACGGCTCCGTTCCAGGAATTCTGGCTGAGGGGGAAACTCTGCTCCGAGTCGGAGTTTCACCGGAAGAGTTACGCTGTGACGTGACCCTGAGGTCAAACCGCTGTGGGGGCCTCCATGATCCTGCTGAGACCAGATCTGGAAGAAGTGTGAACAGCCGGGCGTTAAATAAAGTTTATGAACCAAACTGCAAAAACTGGACTGAACAATAGATTTTTGTTGTTTATATAAGTCAAACAATTTTGTCTGCTGTTGTCTTATTTTGGTTTTCTACATTTGTTTTCACTTTTCCAAAATAGATTTCGACAAAAAGGACAACGCTGCAGACTTTTGTTTTCTTCTGAAATGTTCTTGATTATAAAGTTTTTTTCCTGGAAAATGATCCTTATTTCAGACTCCACCCGCCTTCATTACACTGCAAGGTTCATTTATTCAGTTCCAAACAGGAACGTTTCAAAGGTTCTGGTCACATCCTGGCTCCTTAGACATTTCTGAACAGATTCAAGGATCTTTAATTGTTGGGGTGAAACTAAACGAGTCCCTCTGGTTCTGGGCCGGCCAATCACAGAGCAAATAAAAATATGATTAAAAAAACGGAGCTAGATGCCATAAAAGAGTAGAAAAGCAACACTATAAATATAAACGGGTGTGTTGGTCCGTGGTTGTGGGTAAAGGGGGGTGAGGGGGTCAGCAGTCTTACAGCTTCCGGGTTGAAGCTGCTCCTTTAAGGTGGTGGTCCTGCTCTCTCTGGAGCATCGCTCTGATTGGTTAAAGTATTTGATCATTTTCACTGAGTCAGACGCGTCATTGCGCTTTTGCTGAGTCATGGAAATGGAAAAGTTTCTCATTTCTCTTCCTGTTTTGTGGCTTTACTAGGTTTGGATTTTTAGATGACCTCTTTGGGTTTTTTCTGAGATCAGATCCATTTTGCTGCCGTTACAATGAAGATTTATTTTGATTTCTACATTAAACTGATGAGGACCTGGACTTTGGTTCTGACTCGGTCTTTGGCCACTTTCACACTGGAATATTTGGTCCAGTTTGAAAGGGAATGCTGAGCTTTTGCCTCTTCCTGGTTGAAGAAAGTGTCTTTCTTCTCATGTTCTCGTCTTTGTAGAAGACTGTCTTTGTCTTTTTCCGGGACAGGAGTGTCTATTTGATCCTTTGTTCTTAGGCCTCTGCATGTTGGAGTCTAGAGCCGTGGCTTCAGAGGTTTTTATTTTCCTCCTCCAGGAGAATAGAGGAAGCTTTTGTCTCCTCGGCGTGGGCCTGCACGGACCTGCCTCGTCCAACCTGAATGTCCTGCAGCTGATCTCAACAGGATCATTTTGTCTTAGAAAAACTGGGGCGGAGATTTAGCGACGGATGTTTTTCATTCAGCTGTTGAAACCGTTTGTTCCTTTAAACACAGTTTACGAGGTTTCATCCTGGAAGCGTCCTTACAAAAAAAACAGGGACTTCTACAAGAAAAAGTCCAAGAGCAAAATCCAGATGACTTGTCCATTCGTGCAGGAAGTGAAGTACTGAACCATTAATGAATGTTTTCTGAAGATAATCATTTTGTTATTAAAAAGAATCTATCGGATCAGAACCGGATACAAAAACTTTAAAACTGTAATATGAATTAGGGGGTGAGATTATATAAATTCACTTCTTCCCGCTCCTTTTCAAGCAATAAATCAAACTTTACTAGATTTTAGTTACTGATCACTGTTTTTAATTAAGCAAAATGTGATTTAAGTGACTTTTGTTTGTTAAACTATGCATTTCAACATTTCTGTAATGAGTTTGATAAATATGTGTAAATTCTTTATTGCTTTGACTACAATGCTTGAGATAAATCAATAAATCAAATCAATCAATCAATCACTGCGCAGCACAGACAGAGTGGCGTGTAAATTCAGTCGCGTCTCTTCGTCACAGGACTTCCTCCTCCGCCCTGAAGCACGATGCAGATGCGAAAGTGTCCCTCCGGCGCTCAAATCAGCTGCAGATTGAAGCCGACAGGAAGTGCGGTATTAGTCAGGAGCAATTAAAACCTCAGGAATTCATGCAAACGCCATGAGGCTCAAACACGCCCTGGTCATGGAGGTCAAACACGATCAGACGGTGGAAGTCTGGGTGTGAAACACGTTTATGAGAGAAAACAGGGCAGCTGTGACAAAGAAGAAGAAAAAACAGAAAATAAGCAGGACTGCTGTCCAGCAGGCCTTTGAGGATGCACAGGTACAAACTCGCACAGGTGTGGTGGGAAATTCCTGAGTGGCAGTTCTGTGTTTGACCCAGATTAAAGGAAACAGTTTTCTAAAATCCTCTCATCAAACGTCTGACTGAAGCTGTGAAAGGAAAGAAAAGCTTTAAAACCTCAGGAACACCCCCCCCCCCCCCCCCCCCAAAAACCAANNNNNNNNNNCTCACAAAAACCAGAAGTTTATTTAGTAAAGTACACTTGAGTATAAGTATGGCAAGTATATACAGACCAAGTAAACAAACTGAATACTTCTTCAGACTGGAACGTTTCAGTTTGACTGCTTTCTGCTGCCTTCATATCGTGACTTTATTGTTGTTTCCATCATTCTGAACCTCACGGAGGAACAAGCTGAAACCAGAAAAACATGACAAAAGTGACACTTTTACTTCTTTTTTTTGGTTTTTCTCTCTTTCAAAATACAAATGTGCAGTTTTTGTCTTCCTGTTTCCCACAATTCAGAAGAATAAATCTGAAACCAAATGAACCTGAGACTTGTTTGTCCTTTTTTTTGGATGAAAACGTTTAACTTTTGCGTCTTTTGTTGTGTTTCTGTTTGCAGTCATGAAGCCAGTCGCCCCGATGTGTGAATGTTTGTTTTATGGTCCTATCAGTGCTTCATTTGGATCTTCTGAGGATCAACAAGTGAAAAGGTTGTTGATCTGAATCTTTCTAAAATGTCGACTTCTCCAGCTTGTGGTCGACTGGAGCAGCTGAAGATCCGGTTCATGGAACTGAAAGTTAAAGTTGGACATTTTGAACTCAAAATAAATCAAAGGGTTAACTCTGTATTTTCCGTGTTGTTCCCCCTCCCTGTTGTTGACTCAGATGAGCATCTCTTGTGTGATCATCGCTCTGAGGACTTTCATCAGTGACGTGCTTTGACTTCCTGAACAGATCCAGCCGTTTGGACCAGACCGGGTCTCTGCTGCTGACGCAGTTCTCCTTCGTCGCACACTAACGAGGATGTGAGGAAATCTGAGATTCAGCCGCTGTTGTGCAACAAACAAGCTTTTGAAGACTTGTGAAAACTTTTCTCACAACAAAAGAACCACAAAACTCCATAGGGTTAACCAAAGAGATGAGAAAAGTTTGGAAATAACAGAAGAAAAAGTCTCATTTCTTTAGGTGAGCATTTCCAAATTCACTGCAAGACGTGTTTTCAGATGATCTGGAGTGTTTTTTTTTTTACACGCAATAGTAATGGAAAGGGTTTCTTTCAATCGATCAGAACAAAAAACATCTTTTTGATCAACACAGAAACTAATTTAAAGAATCCTTCGGCTTTTCATCAGTAAAATAACTGCAATGACAGATTTACTCAAAGTCTTGATGCTGAGCTTTAGTTCTACAGCTGGATCAACAAATATCCCTAAAAAAACATTTGACAAACTTTTATTTCCTTTAGACCAAATGGATCATCTCACTAAATGTGAATATATGTTAAAAAAAAAATTTTTTATTGACGACAAGAGATGGAAAAAAAAGTTGTCCACTACAATTCCCAGAATCCCGTACTTTAACATTTGGAGTCTTGAAAAGCCCCAAATGTCCTCTGCAGAAACCAAAAGGAGTCCATTCAATAATCTGCGGTGGTTGGAGATGTTCAGGTGTGGAAATGTCCTCTACAGGGGACACATTTGTGGTAGGCAGGAGGTAAAAGAACTTTAAGACGTGCCCCAGGATACCAATCAAATCCAGAATGCTCTTTTTTTATCTTTAGAAAGAGTTTGCTATTAGCTCCATACATTTTTCTGCACTGGAAACCCTCCATCCCGTTGTTCCATTTGTGTCTCTCAAATATTCTGGAATAAGTGCTGCTGGAGGCACCCACGAACGATTGCATCGATCAGGTCATATGGCGTGCAAACACCTGCACCACAGACCATGCAGAAAGGTTTTCATTAGCATGAGCTGCAAAAGGCAAGAAAGTGTTCATGCAAATGTCAAATGTGATCCAAAATAAAGGCTGTTGCAGAATCCTGTTTTTGACTTTCCTTTTTCTAGATTCAAACCTTTTTCTCCCTTTACTGTTAAAGTTCCAATAATGGGATGACATCCCCAATCAAACTCTGAAATACCACTAAATAAAGTCTTGAAAGATTGAACAGTTTAAATTCTCCTGATCACCAGAGAAATAGCGCGGTGGTGGCAGCATCATGCTGTGTGTGTAGTTTGTTCATATTTAAACGAGTTTATGCATGTTGACAGTCTCCTGAAGTTTTGGGTCCCCCTTCACCGATACCTGACGACGCCTTGGTGTCGTTTAGGCAACATTTCCACCAGAAGATTGGTTTCTTCCTTAAAGTCCCACTCCGATCATCTTCTGATCTCTTCCCAAACCGGTCCCAGTCGTCTTTTCACGATGATGATGCCGCTGTTTTTAGCCAAAATTAAAGAAAAAAGGTGTTTTTTAGGACATAGTTTCTGCAGAGCGGCAGGAGTTTATCAGAAATTCACCTCTGAGTTGTGAACAGGACTGTTCCGCCCCTCTTTCCATCACCTATCTGCATAAACTTTCACCCACTAGCTTACAGCCCCTCAGAACCCCAAGCTAGCATTAGCGGTGCAGCAAAAATGGCTGCAATATCAGATCCATCCATCCGTCCGTCCGTCCAGTTCTGATCCAGATTCCAGCTCAGACGAGGAAAACAAAGACGATCATGGATCTGTTGGTCTGCAGGTGGAGGATCAGAAAGGGCGGAGCAGGGAGACTTTAGCCCCGCCCAGAGTATTTCCTACGATCTTTTTCAAACTGCATTATTTTCGTCTGCTCCTGGTTCACAGCGATTTGATTTGATTTGATTGATCTTTTTCAAGCATTGTAGTCAAAGCAATAAAGAATATACACATATTTATCAAACTCATTACAGAAATGATGAAATGCATAGATTAAAAAGAAAGAAAGAAAAAAACAAAACAAAAACATCACTTAAATCACTTTGCTTAATTAAATACAGCCATAATTAACTAAAGTAAAGTAGAGTTTGATTTATTGCTTGAAAAGGAGCGGGAAGAAGTGAGATTATATAATCCCCCCCCCCGACTGCATTCATTCATTTGATAGTTTTACAGTGTTTTAAATCCGGTTCTGATCCGATTCTCTTCAAGTAACAAAATCCGGTGGCTATTAATGATTGATGATCTTCAGAAAACATTCTTTCATGATTTCAGTAAACGGTAACCATTATGTAATGATCATTGATTATCATTAGAACACATGATCACAATAAACCAGTAATTATTGCTACACATTGCAGATCAAGAAAAGAGAATCAATAGTTATTGGTTGTAATTCAAACCTTTCAGTAATCATTAATGCACATTACACATTAATACTCATTGGTTGTAATTACAACAGCTTTGATTATTTCACCACAATCAAGTTCACACATGGATTTGAATTATTCAAACACCTGTTGATCCTTAAATCTTCTTATCTTCATATCCTGAAATCAGAGTTTCTTTCTACATTTTCTTGAATATTTGAATATTGTTTGAGCTCATTACTTAGACCGTTTAAAGAAGAAATACTCAGACATGCAGATTTTAAGCTGAATTTTCTTCATACATGTCCTCCGTCATCAGAAAAATGCTACAAGAATGTGTTAGAAACCCCAAAAACACTGTTTCCAACAGAGTGGAGCTTTAGGGAGGACTGGAGGACGTATCCAGGACATAATTCCAGGAAGGTCTTTTTTAGTTCAAACATCGTAAAAGATTGTTTTTCCAGGTTAAAGGAGAAATGTGACCAACGGTAAATTATAATCAACCATAAAAGAGTCATAACAGAGTTCACTGCTCTTTTAAAGGGTCTGAATTTTCAGTTTCTCTGCTTTGTTGACAGTGAAACCTTCCTCTCCTGACAGTTTATTGGGAATCATTTGTGATGATTCTCTGTTAACGTCTGTCCCAAAGAACTGACTGAAAGTGGTTCAAAGCCGGATCTTCTAATCCATGAAACCTGTCTGCAGTAAAAAAGCGTGAGTGTGAAGCTGCCTGCAGTGAAGCAACATGTGCAATTACAGCATTTCATCATCGCAGCGAGAGCGGCTGGTAGCCCGTCCCCGTCTGCGGATATAAAAGGCTGCAGGTCAGCACAGGCAGGCAGAACCGCAGCACAGCTCACAGCAAGCACACAGCAACACAGGATCTGACAGGTAACGGGACCTCTCTGCCTTCATCTGCAGGTTTGAGTCCGCAAAGAAGGTTGCTGTTGAAACTAAGAATGTCTCTGTTTGCCGGCACCATGGTGAGCAAGGGCGAGGAGCTGTTCACCGGGGTGGTGCCCATCCTGGTCGAGCTGGACGGCGACGTAAACGGCCACAAGTTCAGCGTGTCCGGCGAGGGCGAGGGCGATGCCACCTACGGCAAGCTGACCCTGAAGTTCATCTGCACCACCGGCAAGCTGCCCGTGCCCTGGCCCACCCTCGTGACCACCCTGACCTACGGCGTGCAGTGCTTCAGCCGCTACCCCGACCACATGAAGCAGCACGACTTCTTCAAGTCCGCCATGCCCGAAGGCTACGTCCAGGAGCGCACCATCTTCTTCAAGGACGACGGCAACTACAAGACCCGCGCCGAGGTGAAGTTCGAGGGCGACACCCTGGTGAACCGCATCGAGCTGAAGGGCATCGACTTCAAGGAGGACGGCAACATCCTGGGGCACAAGCTGGAGTACAACTACAACAGCCACAACGTCTATATCATGGCCGACAAGCAGAAGAACGGCATCAAGGTGAACTTCAAGATCCGCCACAACATCGAGGACGGCAGCGTGCAGCTCGCCGACCACTACCAGCAGAACACCCCCATCGGCGACGGCCCCGTGCTGCTGCCCGACAACCACTACCTGAGCACCCAGTCCGCCCTGAGCAAAGACCCCAACGAGAAGCGCGATCACATGGTCCTGCTGGAGTTCGTGACCGCCGCCGGGATCACTCTCGGCATGGACGAGCTGTACAAGGAGGGCAGAGGAAGTCTTCTAACATGCGGTGACGTGGAGGAGAATCCCGGCCCTATGGAATCTGAGATGGCGTGCCACAAGAGCGACACGTACAGAATGAAGATGTCCCACGGCCTGCATTTGGAGATCTCGCATCATCCTCACTCGATGAGGAGCGTTGTCAATCTCATCATTGCCATTGACCGCCTGAAGGCCGGCGGGATGGAGGAGGTGATGGGTACTGACTTCAGGGACGAGCACCTTCTCAGCGTCATGCTGGAGAGCATTTTGGAAGAGCGACATGTTGCTGAGAGGTCTTCTGCTCCCAGCCAGTTCAGCAGAACCGGGGAGTATCAGTGCAGCGTGACGGACAAGCAGAAGAGGAGCTTGGTTCTGGTCAAGAACAGCATGAAGCTGCTCGCCGTGATGCTGCAGGGAGGCAGCGACAGCCGCAAAGTCCAGCTGAACATGTCTACTTACCTTCACCGGGACCGCACGCCTGAGGCCAGGCCGGTGGCTCTGGGCATCAAGGACACCAAACTCTACCTGTCCTGCCACCAAGAAGGAGACAAACCAACTCTGTTTCTGGAGGAGGTGGAGGACAACAGCACCCTTTCCACAATTGGCCAGGAGAGCGAGAATGTGCGCTTCCTCTTCTACAAACAGGACACGGGTGGAAACCTCAGCACCCTGGCGTCCGCCCGCTTCCCCGGCTGGTACATCAGCACGGCGGCGATCGACAGTGAACCCGTGGAGATGTGCCAGGAGAGCGACAGCCGCTTCAGGACCTTCCACATCCGCCAGAGCGGCGGTTACCCATACGATGTTCCAGATTACGCTTAAACTAGTGACTGACTAGGATCTGGTTACCACTAAACCAGCCTCAAGAACACCCGAATGGAGTCTCTAAGCTACATAATACCNACTTACACTTTACAAAATGTTGTCCCCCAAAATGTAG
GTAAAACGACGGCCAGTGAGCGCGTAGGGATAACAGGGTAATGCGCGCGTAATACGACTCACTATANGGCGAATTGGGTACCGGGCCCCCCCTCGAGGAGGAGGAGTGGAAGTTCCAGAGCGGAGGAAGCAGAAATCATTTATGATCTGTAGTCAGCTGACTAATGTGTGTGTGGGGGAGGGGGGTCTTCTTTTACTACTTTAAACTCTGTCAGCAGTTTTTTCTTCTAGATTTGCTTTTCTATCTGCTTGTAGTTTTTCCTCATTTCTGAATGTGTTCTCCATCTTTGAATCTTCCTTTGTCCTAACAAAATTCTCTGAGGGCCTGAAAATAAAACCCAGTTGGAACTAAAAGCCACCATGATGACACTCCCACCGCCTGCACCGGAACAGGGAGCGACACGCTGAAAGGCCGGGAGCAGAGCCGGAAGTGAGCCGCTTTTGTCTCGGGTTCAATTCCAAAGGTGAGAAATTCCACAGCAGCCCCTCCCAGACGCACCGGGGACTTTTCTGGCTGAAACGGATCGGGGAGGGAATCAGGGCGTGTGCTGCTCAGAGGCCGATGCTCACCTGCACAGCTTTTCCTGACGGCTCCGTTCCAGGAATTCTGGCTGAGGGGGAAACTCTGCTCCGAGTCGGAGTTTCACCGGAAGAGTTACGCTGTGACGTGACCCTGAGGTCAAACCGCTGTGGGGGCCTCCATGATCCTGCTGAGACCAGATCTGGAAGAAGTGTGAACAGCCGGGCGTTAAATAAAGTTTATGAACCAAACTGCAAAAACTGGACTGAACAATAGATTTTTGTTGTTTATATAAGTCAAACAATTTTGTCTGCTGTTGTCTTATTTTGGTTTTCTACATTTGTTTTCACTTTTCCAAAATAGATTTCGACAAAAAGGACAACGCTGCAGACTTTTGTTTTCTTCTGAAATGTTCTTGATTATAAAGTTTTTTTCCTGGAAAATGATCCTTATTTCAGACTCCACCCGCCTTCATTACACTGCAAGGTTCATTTATTCAGTTCCAAACAGGAACGTTTCAAAGGTTCTGGTCACATCCTGGCTCCTTAGACATTTCTGAACAGATTCAAGGATCTTTAATTGTTGGGGTGAAACTAAACGAGTCCCTCTGGTTCTGGGCCGGCCAATCACAGAGCAAATAAAAATATGATTAAAAAAACGGAGCTAGATGCCATAAAAGAGTAGAAAAGCAACACTATAAATATAAACGGGTGTGTTGGTCCGTGGTTGTGGGTAAAGGGGGGTGAGGGGGTCAGCAGTCTTACAGCTTCCGGGTTGAAGCTGCTCCTTTAAGGTGGTGGTCCTGCTCTCTCTGGAGCATCGCTCTGATTGGTTAAAGTATTTGATCATTTTCACTGAGTCAGACGCGTCATTGCGCTTTTGCTGAGTCATGGAAATGGAAAAGTTTCTCATTTCTCTTCCTGTTTTGTGGCTTTACTAGGTTTGGATTTTTAGATGACCTCTTTGGGTTTTTTCTGAGATCAGATCCATTTTGCTGCCGTTACAATGAAGATTTATTTTGATTTCTACATTAAACTGATGAGGACCTGGACTTTGGTTCTGACTCGGTCTTTGGCCACTTTCACACTGGAATATTTGGTCCAGTTTGAAAGGGAATGCTGAGCTTTTGCCTCTTCCTGGTTGAAGAAAGTGTCTTTCTTCTCATGTTCTCGTCTTTGTAGAAGACTGTCTTTGTCTTTTTCCGGGACAGGAGTGTCTATTTGATCCTTTGTTCTTAGGCCTCTGCATGTTGGAGTCTAGAGCCGTGGCTTCAGAGGTTTTTATTTTCCTCCTCCAGGAGAATAGAGGAAGCTTTTGTCTCCTCGGCGTGGGCCTGCACGGACCTGCCTCGTCCAACCTGAATGTCCTGCAGCTGATCTCAACAGGATCATTTTGTCTTAGAAAAACTGGGGCGGAGATTTAGCGACGGATGTTTTTCATTCAGCTGTTGAAACCGTTTGTTCCTTTAAACACAGTTTACGAGGTTTCATCCTGGAAGCGTCCTTACAAAAAAAACAGGGACTTCTACAAGAAAAAGTCCAAGAGCAAAATCCAGATGACTTGTCCATTCGTGCAGGAAGTGAAGTACTGAACCATTAATGAATGTTTTCTGAAGATAATCATTTTGTTATTAAAAAGAATCTATCGGATCAGAACCGGATACAAAAACTTTAAAACTGTAATATGAATTAGGGGGTGAGATTATATAAATTCACTTCTTCCCGCTCCTTTTCAAGCAATAAATCAAACTTTACTAGATTTTAGTTACTGATCACTGTTTTTAATTAAGCAAAATGTGATTTAAGTGACTTTTGTTTGTTAAACTATGCATTTCAACATTTCTGTAATGAGTTTGATAAATATGTGTAAATTCTTTATTGCTTTGACTACAATGCTTGAGATAAATCAATAAATCAAATCAATCAATCAATCACTGCGCAGCACAGACAGAGTGGCGTGTAAATTCAGTCGCGTCTCTTCGTCACAGGACTTCCTCCTCCGCCCTGAAGCACGATGCAGATGCGAAAGTGTCCCTCCGGCGCTCAAATCAGCTGCAGATTGAAGCCGACAGGAAGTGCGGTATTAGTCAGGAGCAATTAAAACCTCAGGAATTCATGCAAACGCCATGAGGCTCAAACACGCCCTGGTCATGGAGGTCAAACACGATCAGACGGTGGAAGTCTGGGTGTGAAACACGTTTATGAGAGAAAACAGGGCAGCTGTGACAAAGAAGAAGAAAAAACAGAAAATAAGCAGGACTGCTGTCCAGCAGGCCTTTGAGGATGCACAGGTACAAACTCGCACAGGTGTGGTGGGAAATTCCTGAGTGGCAGTTCTGTGTTTGACCCAGATTAAAGGAAACAGTTTTCTAAAATCCTCTCATCAAACGTCTGACTGAAGCTGTGAAAGGAAAGAAAAGCTTTAAAACCTCAGGAACACCCCCCCCCCCCCCCCCCCCAAAAACCAANNNNNNNNNNCTCACAAAAACCAGAAGTTTATTTAGTAAAGTACACTTGAGTATAAGTATGGCAAGTATATACAGACCAAGTAAACAAACTGAATACTTCTTCAGACTGGAACGTTTCAGTTTGACTGCTTTCTGCTGCCTTCATATCGTGACTTTATTGTTGTTTCCATCATTCTGAACCTCACGGAGGAACAAGCTGAAACCAGAAAAACATGACAAAAGTGACACTTTTACTTCTTTTTTTTGGTTTTTCTCTCTTTCAAAATACAAATGTGCAGTTTTTGTCTTCCTGTTTCCCACAATTCAGAAGAATAAATCTGAAACCAAATGAACCTGAGACTTGTTTGTCCTTTTTTTTGGATGAAAACGTTTAACTTTTGCGTCTTTTGTTGTGTTTCTGTTTGCAGTCATGAAGCCAGTCGCCCCGATGTGTGAATGTTTGTTTTATGGTCCTATCAGTGCTTCATTTGGATCTTCTGAGGATCAACAAGTGAAAAGGTTGTTGATCTGAATCTTTCTAAAATGTCGACTTCTCCAGCTTGTGGTCGACTGGAGCAGCTGAAGATCCGGTTCATGGAACTGAAAGTTAAAGTTGGACATTTTGAACTCAAAATAAATCAAAGGGTTAACTCTGTATTTTCCGTGTTGTTCCCCCTCCCTGTTGTTGACTCAGATGAGCATCTCTTGTGTGATCATCGCTCTGAGGACTTTCATCAGTGACGTGCTTTGACTTCCTGAACAGATCCAGCCGTTTGGACCAGACCGGGTCTCTGCTGCTGACGCAGTTCTCCTTCGTCGCACACTAACGAGGATGTGAGGAAATCTGAGATTCAGCCGCTGTTGTGCAACAAACAAGCTTTTGAAGACTTGTGAAAACTTTTCTCACAACAAAAGAACCACAAAACTCCATAGGGTTAACCAAAGAGATGAGAAAAGTTTGGAAATAACAGAAGAAAAAGTCTCATTTCTTTAGGTGAGCATTTCCAAATTCACTGCAAGACGTGTTTTCAGATGATCTGGAGTGTTTTTTTTTTTACACGCAATAGTAATGGAAAGGGTTTCTTTCAATCGATCAGAACAAAAAACATCTTTTTGATCAACACAGAAACTAATTTAAAGAATCCTTCGGCTTTTCATCAGTAAAATAACTGCAATGACAGATTTACTCAAAGTCTTGATGCTGAGCTTTAGTTCTACAGCTGGATCAACAAATATCCCTAAAAAAACATTTGACAAACTTTTATTTCCTTTAGACCAAATGGATCATCTCACTAAATGTGAATATATGTTAAAAAAAAAATTTTTTATTGACGACAAGAGATGGAAAAAAAAGTTGTCCACTACAATTCCCAGAATCCCGTACTTTAACATTTGGAGTCTTGAAAAGCCCCAAATGTCCTCTGCAGAAACCAAAAGGAGTCCATTCAATAATCTGCGGTGGTTGGAGATGTTCAGGTGTGGAAATGTCCTCTACAGGGGACACATTTGTGGTAGGCAGGAGGTAAAAGAACTTTAAGACGTGCCCCAGGATACCAATCAAATCCAGAATGCTCTTTTTTTATCTTTAGAAAGAGTTTGCTATTAGCTCCATACATTTTTCTGCACTGGAAACCCTCCATCCCGTTGTTCCATTTGTGTCTCTCAAATATTCTGGAATAAGTGCTGCTGGAGGCACCCACGAACGATTGCATCGATCAGGTCATATGGCGTGCAAACACCTGCACCACAGACCATGCAGAAAGGTTTTCATTAGCATGAGCTGCAAAAGGCAAGAAAGTGTTCATGCAAATGTCAAATGTGATCCAAAATAAAGGCTGTTGCAGAATCCTGTTTTTGACTTTCCTTTTTCTAGATTCAAACCTTTTTCTCCCTTTACTGTTAAAGTTCCAATAATGGGATGACATCCCCAATCAAACTCTGAAATACCACTAAATAAAGTCTTGAAAGATTGAACAGTTTAAATTCTCCTGATCACCAGAGAAATAGCGCGGTGGTGGCAGCATCATGCTGTGTGTGTAGTTTGTTCATATTTAAACGAGTTTATGCATGTTGACAGTCTCCTGAAGTTTTGGGTCCCCCTTCACCGATACCTGACGACGCCTTGGTGTCGTTTAGGCAACATTTCCACCAGAAGATTGGTTTCTTCCTTAAAGTCCCACTCCGATCATCTTCTGATCTCTTCCCAAACCGGTCCCAGTCGTCTTTTCACGATGATGATGCCGCTGTTTTTAGCCAAAATTAAAGAAAAAAGGTGTTTTTTAGGACATAGTTTCTGCAGAGCGGCAGGAGTTTATCAGAAATTCACCTCTGAGTTGTGAACAGGACTGTTCCGCCCCTCTTTCCATCACCTATCTGCATAAACTTTCACCCACTAGCTTACAGCCCCTCAGAACCCCAAGCTAGCATTAGCGGTGCAGCAAAAATGGCTGCAATATCAGATCCATCCATCCGTCCGTCCGTCCAGTTCTGATCCAGATTCCAGCTCAGACGAGGAAAACAAAGACGATCATGGATCTGTTGGTCTGCAGGTGGAGGATCAGAAAGGGCGGAGCAGGGAGACTTTAGCCCCGCCCAGAGTATTTCCTACGATCTTTTTCAAACTGCATTATTTTCGTCTGCTCCTGGTTCACAGCGATTTGATTTGATTTGATTGATCTTTTTCAAGCATTGTAGTCAAAGCAATAAAGAATATACACATATTTATCAAACTCATTACAGAAATGATGAAATGCATAGATTAAAAAGAAAGAAAGAAAAAAACAAAACAAAAACATCACTTAAATCACTTTGCTTAATTAAATACAGCCATAATTAACTAAAGTAAAGTAGAGTTTGATTTATTGCTTGAAAAGGAGCGGGAAGAAGTGAGATTATATAATCCCCCCCCCCGACTGCATTCATTCATTTGATAGTTTTACAGTGTTTTAAATCCGGTTCTGATCCGATTCTCTTCAAGTAACAAAATCCGGTGGCTATTAATGATTGATGATCTTCAGAAAACATTCTTTCATGATTTCAGTAAACGGTAACCATTATGTAATGATCATTGATTATCATTAGAACACATGATCACAATAAACCAGTAATTATTGCTACACATTGCAGATCAAGAAAAGAGAATCAATAGTTATTGGTTGTAATTCAAACCTTTCAGTAATCATTAATGCACATTACACATTAATACTCATTGGTTGTAATTACAACAGCTTTGATTATTTCACCACAATCAAGTTCACACATGGATTTGAATTATTCAAACACCTGTTGATCCTTAAATCTTCTTATCTTCATATCCTGAAATCAGAGTTTCTTTCTACATTTTCTTGAATATTTGAATATTGTTTGAGCTCATTACTTAGACCGTTTAAAGAAGAAATACTCAGACATGCAGATTTTAAGCTGAATTTTCTTCATACATGTCCTCCGTCATCAGAAAAATGCTACAAGAATGTGTTAGAAACCCCAAAAACACTGTTTCCAACAGAGTGGAGCTTTAGGGAGGACTGGAGGACGTATCCAGGACATAATTCCAGGAAGGTCTTTTTTAGTTCAAACATCGTAAAAGATTGTTTTTCCAGGTTAAAGGAGAAATGTGACCAACGGTAAATTATAATCAACCATAAAAGAGTCATAACAGAGTTCACTGCTCTTTTAAAGGGTCTGAATTTTCAGTTTCTCTGCTTTGTTGACAGTGAAACCTTCCTCTCCTGACAGTTTATTGGGAATCATTTGTGATGATTCTCTGTTAACGTCTGTCCCAAAGAACTGACTGAAAGTGGTTCAAAGCCGGATCTTCTAATCCATGAAACCTGTCTGCAGTAAAAAAGCGTGAGTGTGAAGCTGCCTGCAGTGAAGCAACATGTGCAATTACAGCATTTCATCATCGCAGCGAGAGCGGCTGGTAGCCCGTCCCCGTCTGCGGATATAAAAGGCTGCAGGTCAGCACAGGCAGGCAGAACCGCAGCACAGCTCACAGCAAGCACACAGCAACACAGGATCTGACAGGTAACGGGACCTCTCTGCCTTCATCTGCAGGTTTGAGTCCGCAAAGAAGGTTGCTGTTGAAACTAAGAATGTCTCTGTTTGCCGGCACCATGGTGAGCAAGGGCGAGGAGCTGTTCACCGGGGTGGTGCCCATCCTGGTCGAGCTGGACGGCGACGTAAACGGCCACAAGTTCAGCGTGTCCGGCGAGGGCGAGGGCGATGCCACCTACGGCAAGCTGACCCTGAAGTTCATCTGCACCACCGGCAAGCTGCCCGTGCCCTGGCCCACCCTCGTGACCACCCTGACCTACGGCGTGCAGTGCTTCAGCCGCTACCCCGACCACATGAAGCAGCACGACTTCTTCAAGTCCGCCATGCCCGAAGGCTACGTCCAGGAGCGCACCATCTTCTTCAAGGACGACGGCAACTACAAGACCCGCGCCGAGGTGAAGTTCGAGGGCGACACCCTGGTGAACCGCATCGAGCTGAAGGGCATCGACTTCAAGGAGGACGGCAACATCCTGGGGCACAAGCTGGAGTACAACTACAACAGCCACAACGTCTATATCATGGCCGACAAGCAGAAGAACGGCATCAAGGTGAACTTCAAGATCCGCCACAACATCGAGGACGGCAGCGTGCAGCTCGCCGACCACTACCAGCAGAACACCCCCATCGGCGACGGCCCCGTGCTGCTGCCCGACAACCACTACCTGAGCACCCAGTCCGCCCTGAGCAAAGACCCCAACGAGAAGCGCGATCACATGGTCCTGCTGGAGTTCGTGACCGCCGCCGGGATCACTCTCGGCATGGACGAGCTGTACAAGGAGGGCAGAGGAAGTCTTCTAACATGCGGTGACGTGGAGGAGAATCCCGGCCCTATGGAATCTGAGATGGCGTGCCACAAGAGCGACACGTACAGAATGAAGATGTCCCACGGCCTGCATTTGGAGATCTCGCATCATCCTCACTCGATGAGGAGCGTTGTCAATCTCATCATTGCCATTGACCGCCTGAAGGCCGGCGGGATGGAGGAGGTGATGGGTACTGACTTCAGGGACGAGCACCTTCTCAGCGTCATGCTGGAGAGCATTTTGGAAGAGCGACATGTTGCTGAGAGGTCTTCTGCTCCCAGCCAGTTCAGCAGAACCGGGGAGTATCAGTGCAGCGTGACGGACAAGCAGAAGAGGAGCTTGGTTCTGGTCAAGAACAGCATGAAGCTGCTCGCCGTGATGCTGCAGGGAGGCAGCGACAGCCGCAAAGTCCAGCTGAACATGTCTACTTACCTTCACCGGGACCGCACGCCTGAGGCCAGGCCGGTGGCTCTGGGCATCAAGGACACCAAACTCTACCTGTCCTGCCACCAAGAAGGAGACAAACCAACTCTGTTTCTGGAGGAGGTGGAGGACAACAGCACCCTTTCCACAATTGGCCAGGAGAGCGAGAATGTGCGCTTCCTCTTCTACAAACAGGACACGGGTGGAAACCTCAGCACCCTGGCGTCCGCCCGCTTCCCCGGCTGGTACATCAGCACGGCGGCGATCGACAGTGAACCCGTGGAGATGTGCCAGGAGAGCGACAGCCGCTTCAGGACCTTCCACATCCGCCAGAGCGGCGGTTACCCATACGATGTTCCAGATTACGCTTAAACTAGTGACTGACTAGGATCTGGTTACCACTAAACCAGCCTCAAGAACACCCGAATGGAGTCTCTAAGCTACATAATACCNACTTACACTTTACAAAATGTTGTCCCCCAAAATGTAG
