EPR Plasmid #457: pAP27
Plasmid Details
| Purpose | Generation of Knock-In donors containing mNeonGreen (Linker::3xFlag::mNeonGreen::HAtag::Linker). Insert in pUC19, no expression sequence |
| Depositing Lab |
Alexandre Paix European Molecular Biology Laboratory (EMBL) |
| Depositing Scientist | Alexandre Paix |
| Antibiotic Resistance | Ampicillin |
| Bacterial Growth Temp | 37 |
| Copy Number | High |
| Selectable Marker | |
| Reference |
Endogenous protein tagging in medaka using a simplified CRISPR/Cas9 knock-in approach. Seleit A et al. Elife 2021 PubMed DOI See other plasmids from this publication |
| MTA and Terms |
MTA (PDF download) |
How to Cite This Reagent
IN THE MATERIALS & METHODS:
pAP27 was a gift from Alexandre Paix (European Plasmid Repository plasmid #457).
pAP27 was a gift from Alexandre Paix (European Plasmid Repository plasmid #457).
IN THE REFERENCES:
Seleit A et al.. Endogenous protein tagging in medaka using a simplified CRISPR/Cas9 knock-in approach.. Elife 2021 doi:10.7554/eLife.75050 PubMed 34870593
Seleit A et al.. Endogenous protein tagging in medaka using a simplified CRISPR/Cas9 knock-in approach.. Elife 2021 doi:10.7554/eLife.75050 PubMed 34870593
Sequences
Provided by depositor
download sequence
TCTAGCGGACCTTCAGGTAGTTCGAGCGACTACAAGGACCACGACGGGGATTATAAAGATCATGATATAGATTATAAAGATGATGATGATAAAGTCTCCAAAGGGGAGGAAGACAACATGGCGTCCCTCCCAGCGACGCACGAACTCCATATCTTTGGGTCCATAAACGGTGTCGATTTCGACATGGTAGGACAAGGGACCGGAAATCCAAATGATGGATATGAGGAGCTAAACCTCAAGAGTACCAAGGGGGACCTCCAATTTTCCCCATGGATACTCGTCCCCCATATCGGGTACGGATTCCATCAATACCTACCATATCCAGATGGGATGTCCCCATTTCAAGCGGCCATGGTAGACGGAAGTGGATACCAAGTACATCGTACGATGCAATTTGAGGACGGGGCCTCCCTAACCGTCAACTATCGTTACACGTACGAGGGAAGTCACATCAAAGGAGAGGCGCAAGTCAAAGGAACTGGATTTCCAGCCGATGGACCAGTCATGACGAATTCCCTAACCGCCGCCGATTGGTGTCGTAGTAAAAAGACGTACCCAAATGACAAGACCATAATCTCCACGTTCAAGTGGTCCTATACCACCGGGAACGGAAAGCGGTACCGTAGTACGGCCCGGACGACGTACACGTTCGCCAAGCCCATGGCCGCTAATTACCTGAAGAATCAGCCAATGTATGTCTTCCGTAAAACCGAGCTAAAGCACTCCAAAACCGAGCTAAACTTCAAGGAATGGCAAAAGGCGTTCACGGACGTAATGGGAATGGACGAACTCTACAAGTACCCATATGATGTCCCGGATTACGCTAGCTCTGGTCCAAGTGGATCAAGCTCG
TCTAGCGGACCTTCAGGTAGTTCGAGCGACTACAAGGACCACGACGGGGATTATAAAGATCATGATATAGATTATAAAGATGATGATGATAAAGTCTCCAAAGGGGAGGAAGACAACATGGCGTCCCTCCCAGCGACGCACGAACTCCATATCTTTGGGTCCATAAACGGTGTCGATTTCGACATGGTAGGACAAGGGACCGGAAATCCAAATGATGGATATGAGGAGCTAAACCTCAAGAGTACCAAGGGGGACCTCCAATTTTCCCCATGGATACTCGTCCCCCATATCGGGTACGGATTCCATCAATACCTACCATATCCAGATGGGATGTCCCCATTTCAAGCGGCCATGGTAGACGGAAGTGGATACCAAGTACATCGTACGATGCAATTTGAGGACGGGGCCTCCCTAACCGTCAACTATCGTTACACGTACGAGGGAAGTCACATCAAAGGAGAGGCGCAAGTCAAAGGAACTGGATTTCCAGCCGATGGACCAGTCATGACGAATTCCCTAACCGCCGCCGATTGGTGTCGTAGTAAAAAGACGTACCCAAATGACAAGACCATAATCTCCACGTTCAAGTGGTCCTATACCACCGGGAACGGAAAGCGGTACCGTAGTACGGCCCGGACGACGTACACGTTCGCCAAGCCCATGGCCGCTAATTACCTGAAGAATCAGCCAATGTATGTCTTCCGTAAAACCGAGCTAAAGCACTCCAAAACCGAGCTAAACTTCAAGGAATGGCAAAAGGCGTTCACGGACGTAATGGGAATGGACGAACTCTACAAGTACCCATATGATGTCCCGGATTACGCTAGCTCTGGTCCAAGTGGATCAAGCTCG
