EPR Plasmid #566: GA06_CMV-Mb3Cas12a-hU6-BbsI-CMV-GFP
Plasmid Details
| Purpose | Expression of Mb3Cas12a and one gRNA under a hU6 promoter. BbsI entry site to incorporate gRNA. Expression as eGFP as reporter. |
| Depositing Lab |
Ana Martin-Villalba Deutsches Krebsforschungszentrum (DKFZ) |
| Depositing Scientist | Katrin Volk |
| Antibiotic Resistance | Ampicillin |
| Bacterial Growth Temp | 37 |
| Copy Number | High |
| Selectable Marker | |
| Reference |
This plasmid is from an unpublished project. Please acknowledge the depositing scientist.
See other plasmids from this project |
| MTA and Terms |
MTA (PDF download) |
How to Cite This Reagent
IN THE MATERIALS & METHODS:
GA06_CMV-Mb3Cas12a-hU6-BbsI-CMV-GFP was a gift from Ana Martin-Villalba (European Plasmid Repository plasmid #566).
GA06_CMV-Mb3Cas12a-hU6-BbsI-CMV-GFP was a gift from Ana Martin-Villalba (European Plasmid Repository plasmid #566).
IN THE REFERENCES:
No reference available. (This plasmid is not yet published.)
No reference available. (This plasmid is not yet published.)
Sequences
Provided by depositor
download sequence
cctagagctcgctgatcagcctcgactgtgccttctagttgccagccatctgttgtttgcccctcccccgtgccttccttgaccctggaaggtgccactcccactgtcctttcctaataaaatgaggaaattgcatcgcattgtctgagtaggtgtcattctattctggggggtggggtggggcaggacagcaagggggaggattgggaagagaatagcaggcatgctggggagcggccgccgcgttgacattgattattgactagttattaatagtaatcaattacggggtcattagttcatagcccatatatggagttccgcgttacataacttacggtaaatggcccgcctggctgaccgcccaacgacccccgcccattgacgtcaataatgacgtatgttcccatagtaacgccaatagggactttccattgacgtcaatgggtggagtatttacggtaaactgcccacttggcagtacatcaagtgtatcatatgccaagtacgccccctattgacgtcaatgacggtaaatggcccgcctggcattatgcccagtacatgaccttatgggactttcctacttggcagtacatctacgtattagtcatcgctattaccatggtgatgcggttttggcagtacatcaatgggcgtggatagcggtttgactcacggggatttccaagtctccaccccattgacgtcaatgggagtttgttttggcaccaaaatcaacgggactttccaaaatgtcgtaacaactccgccccattgacgcaaatgggcggtaggcgtgtacggtgggaggtctatataagcagagctctctggctaactagaGAACCCACTGCGCCGCCACCATGGTGAGCAAGGGCGAGGAGCTGTTCACCGGGGTGGTGCCCATCCTGGTCGAGCTGGACGGCGACGTAAACGGCCACAAGTTCAGCGTGTCCGGCGAGGGCGAGGGCGATGCCACCTACGGCAAGCTGACCCTGAAGTTCATCTGCACCACCGGCAAGCTGCCCGTGCCCTGGCCCACCCTCGTGACCACCCTGACCTACGGCGTGCAGTGCTTCAGCCGCTACCCCGACCACATGAAGCAGCACGACTTCTTCAAGTCCGCCATGCCCGAAGGCTACGTCCAGGAGCGCACCATCTTCTTCAAGGACGACGGCAACTACAAGACCCGCGCCGAGGTGAAGTTCGAGGGCGACACCCTGGTGAACCGCATCGAGCTGAAGGGCATCGACTTCAAGGAGGACGGCAACATCCTGGGGCACAAGCTGGAGTACAACTACAACAGCCACAACGTCTATATCATGGCCGACAAGCAGAAGAACGGCATCAAGGTGAACTTCAAGATCCGCCACAACATCGAGGACGGCAGCGTGCAGCTCGCCGACCACTACCAGCAGAACACCCCCATCGGCGACGGCCCCGTGCTGCTGCCCGACAACCACTACCTGAGCACCCAGTCCGCCCTGAGCAAAGACCCCAACGAGAAGCGCGATCACATGGTCCTGCTGGAGTTCGTGACCGCCGCCGGGATCACTCTCGGCATGGACGAGCTGTACAAGTAAgagcagacatgataagatacattgatgagtttggacaaaccacaactagaatgcagtgaaaaaaatgctttatttgtgaaatttgtgatgctattgctttatttgtaaccattataagctgcaataaacaagttaacaacaacaattgcattcattttatgtttcaggttcagggggaggtgtgggaggttttttaaagcaagtaaaacctctacaaatgtggtagcggccgcaggaacccctagtgatggagttggccactccctctctgcgcgctcgctcgctcactgaggccgggcgaccaaaggtcgcccgacgcccgggctttgcccgggcggcctcagtgagcgagcgagcgcgcagctgcctgcaggggcgcctgatgcggtattttctccttacgcatctgtgcggtatttcacaccgcatacgtcaaagcaaccatagtacgcgccctgtagcggcgcattaagcgcggcgggtgtggtggttacgcgcagcgtgaccgctacacttgccagcgccttagcgcccgctcctttcgctttcttcccttcctttctcgccacgttcgccggctttccccgtcaagctctaaatcgggggctccctttagggttccgatttagtgctttacggcacctcgaccccaaaaaacttgatttgggtgatggttcacgtagtgggccatcgccctgatagacggtttttcgccctttgacgttggagtccacgttctttaatagtggactcttgttccaaactggaacaacactcaactctatctcgggctattcttttgatttataagggattttgccgatttcggtctattggttaaaaaatgagctgatttaacaaaaatttaacgcgaattttaacaaaatattaacgtttacaattttatggtgcactctcagtacaatctgctctgatgccgcatagttaagccagccccgacacccgccaacacccgctgacgcgccctgacgggcttgtctgctcccggcatccgcttacagacaagctgtgaccgtctccgggagctgcatgtgtcagaggttttcaccgtcatcaccgaaacgcgcgagacgaaagggcctcgtgatacgcctatttttataggttaatgtcatgataataatggtttcttagacgtcaggtggcacttttcggggaaatgtgcgcggaacccctatttgtttatttttctaaatacattcaaatatgtatccgctcatgagacaataaccctgataaatgcttcaataatattgaaaaaggaagagtatgagtattcaacatttccgtgtcgcccttattcccttttttgcggcattttgccttcctgtttttgctcacccagaaacgctggtgaaagtaaaagatgctgaagatcagttgggtgcacgagtgggttacatcgaactggatctcaacagcggtaagatccttgagagttttcgccccgaagaacgttttccaatgatgagcacttttaaagttctgctatgtggcgcggtattatcccgtattgacgccgggcaagagcaactcggtcgccgcatacactattctcagaatgacttggttgagtactcaccagtcacagaaaagcatcttacggatggcatgacagtaagagaattatgcagtgctgccataaccatgagtgataacactgcggccaacttacttctgacaacgatcggaggaccgaaggagctaaccgcttttttgcacaacatgggggatcatgtaactcgccttgatcgttgggaaccggagctgaatgaagccataccaaacgacgagcgtgacaccacgatgcctgtagcaatggcaacaacgttgcgcaaactattaactggcgaactacttactctagcttcccggcaacaattaatagactggatggaggcggataaagttgcaggaccacttctgcgctcggcccttccggctggctggtttattgctgataaatctggagccggtgagcgtggaagccgcggtatcattgcagcactggggccagatggtaagccctcccgtatcgtagttatctacacgacggggagtcaggcaactatggatgaacgaaatagacagatcgctgagataggtgcctcactgattaagcattggtaactgtcagaccaagtttactcatatatactttagattgatttaaaacttcatttttaatttaaaaggatctaggtgaagatcctttttgataatctcatgaccaaaatcccttaacgtgagttttcgttccactgagcgtcagaccccgtagaaaagatcaaaggatcttcttgagatcctttttttctgcgcgtaatctgctgcttgcaaacaaaaaaaccaccgctaccagcggtggtttgtttgccggatcaagagctaccaactctttttccgaaggtaactggcttcagcagagcgcagataccaaatactgttcttctagtgtagccgtagttaggccaccacttcaagaactctgtagcaccgcctacatacctcgctctgctaatcctgttaccagtggctgctgccagtggcgataagtcgtgtcttaccgggttggactcaagacgatagttaccggataaggcgcagcggtcgggctgaacggggggttcgtgcacacagcccagcttggagcgaacgacctacaccgaactgagatacctacagcgtgagctatgagaaagcgccacgcttcccgaagggagaaaggcggacaggtatccggtaagcggcagggtcggaacaggagagcgcacgagggagcttccagggggaaacgcctggtatctttatagtcctgtcgggtttcgccacctctgacttgagcgtcgatttttgtgatgctcgtcaggggggcggagcctatggaaaaacgccagcaacgcggcctttttacggttcctggccttttgctggccttttgctcagctagcGTCATCATTAGTGTgTAcATGCCACCCCGAAAATACCGCCTAGAAATGTCTATGATTGAAAACGACTGCTAAATCCGCGTGACTCGAGgagggcctatttcccatgattccttcatatttgcatatacgatacaaggctgttagagagataattggaattaatttgactgtaaacacaaagatattagtacaaaatacgtgacgtagaaagtaataatttcttgggtagtttgcagttttaaaattatgttttaaaatggactatcatatgcttaccgtaacttgaaagtatttcgatttcttggctttatatatcttgtggaaaggacgaaacaccgggtcttcgagaagacctTTTTTTTTTATCTCTCCGTCCGAGCACGATAGAGAACgccgcgttgacattgattattgactagttattaatagtaatcaattacggggtcattagttcatagcccatatatggagttccgcgttacataacttacggtaaatggcccgcctggctgaccgcccaacgacccccgcccattgacgtcaataatgacgtatgttcccatagtaacgccaatagggactttccattgacgtcaatgggtggagtatttacggtaaactgcccacttggcagtacatcaagtgtatcatatgccaagtacgccccctattgacgtcaatgacggtaaatggcccgcctggcattatgcccagtacatgaccttatgggactttcctacttggcagtacatctacgtattagtcatcgctattaccatggtgatgcggttttggcagtacatcaatgggcgtggatagcggtttgactcacggggatttccaagtctccaccccattgacgtcaatgggagtttgttttggcaccaaaatcaacgggactttccaaaatgtcgtaacaactccgccccattgacgcaaatgggcggtaggcgtgtacggtgggaggtctatataagcagagctcctctggctaactaccggtcGCCACCatgctgttccaggacttcacccacctgtaccccctgagcaagaccgtcagattcgagctgaagcccatcggcaagaccctggaacacatccacgccaagaactttctgaaccaggacgagacaatggccgacatgtaccagaaagtgaaggccatcctggacgactaccaccgggactttatcgccgacatgatgggcgaagtgaagctgaccaagctggccgagttctacgacgtgtacctgaagttccggaagaaccccaaggacgacggcctgcagaagcagctgaaagacctgcaggctgtgctgcggaaagaaatcgtgaagcctatcggaaacggcggcaagtacaaggccggctacgacagactgttcggcgccaagctgttcaaggacggcaaagagctgggcgacctggccaagttcgtgatcgcccaggaaggcgagagcagccctaagctggcccacctggcccatttcgagaagttcagcacctacttcaccggcttccacgacaaccgcaagaacatgtacagcgacgaggacaagcacaccgccattgcctaccggctgatccacgagaacctgccccggttcatcgacaacctgcagatcctggccaccatcaagcagaagcacagcgccctgtacgaccagatcatcaacgagctgaccgccagcggcctggatgtgtctctggcctctcacctggatggctaccacaagctgctgacacaggaaggcatcaccgcctacaacaccctgctgggcggaatctctggcgaggccggcagcagaaagatccagggcatcaatgaactgatcaacagccaccacaaccagcactgccacaagagcgagcggattgccaagctgcggcccctgcacaagcagattctgagcgacggaatgggcgtgtccttcctgcccagcaagttcgccgacgacagcgaagtgtgccaggccgtgaatgagttctaccggcactacgccgacgtgttcgccaaggtgcagagcctgttcgacggcttcgacgactatcagaaagacggcatctacgtggagtacaagaacctgaacgagctgtccaagcaggccttcggcgacttcgctctgctgggaagagtgctggacggctactatgtggacgtcgtgaaccccgagttcaacgagagatttgccaaggccaagaccgacaacgccaaagccaagctgacaaaagagaaggacaagttcatcaagggcgtgcactccctggcttctctggaacaggccatcgagcactacaccgccagacacgacgacgagtctgtgcaggccggaaagctgggccagtacttcaagcacggactggccggcgtggacaaccccatccagaagatccacaacaaccactctacaatcaagggcttcctggaaagagagcggcctgccggcgaaagagccctgcccaagatcaagagcgacaagagccccgagatcagacagctgaaagagctgctggacaacgccctgaacgtggcccacttcgccaaactgctgaccaccaagaccacactgcacaatcaggatggcaacttctacggcgagttcggagccctgtatgatgagctggccaagatcgccaccctgtacaacaaagtgcgggactacctgagccagaagcccttcagcaccgagaagtacaagctgaatttcggcaaccctaccctgctgaacggctgggacctgaacaaagagaaagataacttcggcgtgatcctgcagaaggacggctgctactacctggccctgctggataaggcccacaagaaggtgttcgataacgcccccaacaccggcaagtctgtgtatcagaagatgatctataagctgctgcctggccccaacaagatgctgcccaaggtgttcttcgccaagagcaacctggactactacaaccccagcgccgaactgctggacaaatacgcccagggcacacacaagaaaggcgacaacttcaacctgaaggactgccacgccctgatcgatttcttcaaggccggaatcaacaagcaccccgagtggcagcacttcggcttcaagttcagccccaccagcagctaccaggacctgagcgacttctacagagaggtggaaccccagggctaccaagtgaagttcgtggacatcaacgccgactacatcaatgagctggtggaacagggccagctgtacctgtttcagatctacaacaaggactttagccccaaggcccacggcaagcccaacctgcacaccctgtatttcaaggccctgtttagcgaggacaacctcgtgaatcccatctacaaactgaacggggaggccgagatcttctaccggaaggcctccctggacatgaacgagacaaccatccacagagccggcgaggtgctggaaaacaagaaccctgacaaccctaagaaacggcagttcgtgtacgacatcatcaaggacaagcggtacacccaggataagttcatgctgcacgtgcccatcaccatgaacttcggagtgcagggcatgaccatcaaagagttcaacaaaaaagtgaaccagagcatccagcagtacgacgaagtgaacgtgatcggcatcgaccggggcgagcggcatctgctgtatctgaccgtgatcaactccaagggcgagattctggaacagagatccctgaacgacatcaccaccgcctccgccaacggcacccagatgaccaccccttaccacaagatcctggacaagcgcgagatcgagcggctgaacgccagagtgggatggggagagatcgagacaatcaaagaactgaagtccggctacctgtcccacgtggtgcatcagatctcccagctgatgctgaagtacaacgccatcgtggtgctggaagatctgaattttggcttcaagaggggccggttcaaggtggaaaagcagatctaccagaacttcgagaatgccctgatcaagaaactgaaccacctggtgctgaaagacaaggccgacgacgagatcggcagctacaagaacgccctgcagctgactaacaacttcaccgatctgaagagtatcggcaagcagaccggctttctgttctacgtgcccgcctggaataccagcaagatcgaccccgagacaggcttcgtggacctgctgaagcctagatacgagaatatcgcccagagccaggccttcttcggcaagttcgacaagatctgctacaacgccgataggggctacttcgagttccacatcgactacgccaagttcaatgacaaggccaaaaacagccggcagatctggaaaatctgcagccacggcgataagcgctacgtgtacgataagaccgccaaccagaacaagggcgccaccatcggagtgaatgtgaacgatgagctgaagtccctgttcacccgctaccacatcaacgataagcagcccaatctcgtgatggacatctgccagaacaacgacaaagagtttcacaagagcctgatgtatctgctgaaaacactgctggctctgcggtacagcaacgccagctccgacgaggatttcatcctgagccccgtggccaacgacgagggcgtgttcttcaatagcgccctggccgacgatacccagccccagaatgccgatgccaacggcgcctaccacattgccctgaagggactgtggctgctgaacgaactgaagaacagcgacgatctgaacaaagtgaagctggccatcgacaaccagacctggctgaatttcgcccagaacagaaagaggccggcggccacgaagaaggccggccaggccaagaagaagaagggatcctacccatacgatgttccagattacgcttatccctacgacgtgcctgattatgcatacccatatgatgtccccgactatgcctaa
cctagagctcgctgatcagcctcgactgtgccttctagttgccagccatctgttgtttgcccctcccccgtgccttccttgaccctggaaggtgccactcccactgtcctttcctaataaaatgaggaaattgcatcgcattgtctgagtaggtgtcattctattctggggggtggggtggggcaggacagcaagggggaggattgggaagagaatagcaggcatgctggggagcggccgccgcgttgacattgattattgactagttattaatagtaatcaattacggggtcattagttcatagcccatatatggagttccgcgttacataacttacggtaaatggcccgcctggctgaccgcccaacgacccccgcccattgacgtcaataatgacgtatgttcccatagtaacgccaatagggactttccattgacgtcaatgggtggagtatttacggtaaactgcccacttggcagtacatcaagtgtatcatatgccaagtacgccccctattgacgtcaatgacggtaaatggcccgcctggcattatgcccagtacatgaccttatgggactttcctacttggcagtacatctacgtattagtcatcgctattaccatggtgatgcggttttggcagtacatcaatgggcgtggatagcggtttgactcacggggatttccaagtctccaccccattgacgtcaatgggagtttgttttggcaccaaaatcaacgggactttccaaaatgtcgtaacaactccgccccattgacgcaaatgggcggtaggcgtgtacggtgggaggtctatataagcagagctctctggctaactagaGAACCCACTGCGCCGCCACCATGGTGAGCAAGGGCGAGGAGCTGTTCACCGGGGTGGTGCCCATCCTGGTCGAGCTGGACGGCGACGTAAACGGCCACAAGTTCAGCGTGTCCGGCGAGGGCGAGGGCGATGCCACCTACGGCAAGCTGACCCTGAAGTTCATCTGCACCACCGGCAAGCTGCCCGTGCCCTGGCCCACCCTCGTGACCACCCTGACCTACGGCGTGCAGTGCTTCAGCCGCTACCCCGACCACATGAAGCAGCACGACTTCTTCAAGTCCGCCATGCCCGAAGGCTACGTCCAGGAGCGCACCATCTTCTTCAAGGACGACGGCAACTACAAGACCCGCGCCGAGGTGAAGTTCGAGGGCGACACCCTGGTGAACCGCATCGAGCTGAAGGGCATCGACTTCAAGGAGGACGGCAACATCCTGGGGCACAAGCTGGAGTACAACTACAACAGCCACAACGTCTATATCATGGCCGACAAGCAGAAGAACGGCATCAAGGTGAACTTCAAGATCCGCCACAACATCGAGGACGGCAGCGTGCAGCTCGCCGACCACTACCAGCAGAACACCCCCATCGGCGACGGCCCCGTGCTGCTGCCCGACAACCACTACCTGAGCACCCAGTCCGCCCTGAGCAAAGACCCCAACGAGAAGCGCGATCACATGGTCCTGCTGGAGTTCGTGACCGCCGCCGGGATCACTCTCGGCATGGACGAGCTGTACAAGTAAgagcagacatgataagatacattgatgagtttggacaaaccacaactagaatgcagtgaaaaaaatgctttatttgtgaaatttgtgatgctattgctttatttgtaaccattataagctgcaataaacaagttaacaacaacaattgcattcattttatgtttcaggttcagggggaggtgtgggaggttttttaaagcaagtaaaacctctacaaatgtggtagcggccgcaggaacccctagtgatggagttggccactccctctctgcgcgctcgctcgctcactgaggccgggcgaccaaaggtcgcccgacgcccgggctttgcccgggcggcctcagtgagcgagcgagcgcgcagctgcctgcaggggcgcctgatgcggtattttctccttacgcatctgtgcggtatttcacaccgcatacgtcaaagcaaccatagtacgcgccctgtagcggcgcattaagcgcggcgggtgtggtggttacgcgcagcgtgaccgctacacttgccagcgccttagcgcccgctcctttcgctttcttcccttcctttctcgccacgttcgccggctttccccgtcaagctctaaatcgggggctccctttagggttccgatttagtgctttacggcacctcgaccccaaaaaacttgatttgggtgatggttcacgtagtgggccatcgccctgatagacggtttttcgccctttgacgttggagtccacgttctttaatagtggactcttgttccaaactggaacaacactcaactctatctcgggctattcttttgatttataagggattttgccgatttcggtctattggttaaaaaatgagctgatttaacaaaaatttaacgcgaattttaacaaaatattaacgtttacaattttatggtgcactctcagtacaatctgctctgatgccgcatagttaagccagccccgacacccgccaacacccgctgacgcgccctgacgggcttgtctgctcccggcatccgcttacagacaagctgtgaccgtctccgggagctgcatgtgtcagaggttttcaccgtcatcaccgaaacgcgcgagacgaaagggcctcgtgatacgcctatttttataggttaatgtcatgataataatggtttcttagacgtcaggtggcacttttcggggaaatgtgcgcggaacccctatttgtttatttttctaaatacattcaaatatgtatccgctcatgagacaataaccctgataaatgcttcaataatattgaaaaaggaagagtatgagtattcaacatttccgtgtcgcccttattcccttttttgcggcattttgccttcctgtttttgctcacccagaaacgctggtgaaagtaaaagatgctgaagatcagttgggtgcacgagtgggttacatcgaactggatctcaacagcggtaagatccttgagagttttcgccccgaagaacgttttccaatgatgagcacttttaaagttctgctatgtggcgcggtattatcccgtattgacgccgggcaagagcaactcggtcgccgcatacactattctcagaatgacttggttgagtactcaccagtcacagaaaagcatcttacggatggcatgacagtaagagaattatgcagtgctgccataaccatgagtgataacactgcggccaacttacttctgacaacgatcggaggaccgaaggagctaaccgcttttttgcacaacatgggggatcatgtaactcgccttgatcgttgggaaccggagctgaatgaagccataccaaacgacgagcgtgacaccacgatgcctgtagcaatggcaacaacgttgcgcaaactattaactggcgaactacttactctagcttcccggcaacaattaatagactggatggaggcggataaagttgcaggaccacttctgcgctcggcccttccggctggctggtttattgctgataaatctggagccggtgagcgtggaagccgcggtatcattgcagcactggggccagatggtaagccctcccgtatcgtagttatctacacgacggggagtcaggcaactatggatgaacgaaatagacagatcgctgagataggtgcctcactgattaagcattggtaactgtcagaccaagtttactcatatatactttagattgatttaaaacttcatttttaatttaaaaggatctaggtgaagatcctttttgataatctcatgaccaaaatcccttaacgtgagttttcgttccactgagcgtcagaccccgtagaaaagatcaaaggatcttcttgagatcctttttttctgcgcgtaatctgctgcttgcaaacaaaaaaaccaccgctaccagcggtggtttgtttgccggatcaagagctaccaactctttttccgaaggtaactggcttcagcagagcgcagataccaaatactgttcttctagtgtagccgtagttaggccaccacttcaagaactctgtagcaccgcctacatacctcgctctgctaatcctgttaccagtggctgctgccagtggcgataagtcgtgtcttaccgggttggactcaagacgatagttaccggataaggcgcagcggtcgggctgaacggggggttcgtgcacacagcccagcttggagcgaacgacctacaccgaactgagatacctacagcgtgagctatgagaaagcgccacgcttcccgaagggagaaaggcggacaggtatccggtaagcggcagggtcggaacaggagagcgcacgagggagcttccagggggaaacgcctggtatctttatagtcctgtcgggtttcgccacctctgacttgagcgtcgatttttgtgatgctcgtcaggggggcggagcctatggaaaaacgccagcaacgcggcctttttacggttcctggccttttgctggccttttgctcagctagcGTCATCATTAGTGTgTAcATGCCACCCCGAAAATACCGCCTAGAAATGTCTATGATTGAAAACGACTGCTAAATCCGCGTGACTCGAGgagggcctatttcccatgattccttcatatttgcatatacgatacaaggctgttagagagataattggaattaatttgactgtaaacacaaagatattagtacaaaatacgtgacgtagaaagtaataatttcttgggtagtttgcagttttaaaattatgttttaaaatggactatcatatgcttaccgtaacttgaaagtatttcgatttcttggctttatatatcttgtggaaaggacgaaacaccgggtcttcgagaagacctTTTTTTTTTATCTCTCCGTCCGAGCACGATAGAGAACgccgcgttgacattgattattgactagttattaatagtaatcaattacggggtcattagttcatagcccatatatggagttccgcgttacataacttacggtaaatggcccgcctggctgaccgcccaacgacccccgcccattgacgtcaataatgacgtatgttcccatagtaacgccaatagggactttccattgacgtcaatgggtggagtatttacggtaaactgcccacttggcagtacatcaagtgtatcatatgccaagtacgccccctattgacgtcaatgacggtaaatggcccgcctggcattatgcccagtacatgaccttatgggactttcctacttggcagtacatctacgtattagtcatcgctattaccatggtgatgcggttttggcagtacatcaatgggcgtggatagcggtttgactcacggggatttccaagtctccaccccattgacgtcaatgggagtttgttttggcaccaaaatcaacgggactttccaaaatgtcgtaacaactccgccccattgacgcaaatgggcggtaggcgtgtacggtgggaggtctatataagcagagctcctctggctaactaccggtcGCCACCatgctgttccaggacttcacccacctgtaccccctgagcaagaccgtcagattcgagctgaagcccatcggcaagaccctggaacacatccacgccaagaactttctgaaccaggacgagacaatggccgacatgtaccagaaagtgaaggccatcctggacgactaccaccgggactttatcgccgacatgatgggcgaagtgaagctgaccaagctggccgagttctacgacgtgtacctgaagttccggaagaaccccaaggacgacggcctgcagaagcagctgaaagacctgcaggctgtgctgcggaaagaaatcgtgaagcctatcggaaacggcggcaagtacaaggccggctacgacagactgttcggcgccaagctgttcaaggacggcaaagagctgggcgacctggccaagttcgtgatcgcccaggaaggcgagagcagccctaagctggcccacctggcccatttcgagaagttcagcacctacttcaccggcttccacgacaaccgcaagaacatgtacagcgacgaggacaagcacaccgccattgcctaccggctgatccacgagaacctgccccggttcatcgacaacctgcagatcctggccaccatcaagcagaagcacagcgccctgtacgaccagatcatcaacgagctgaccgccagcggcctggatgtgtctctggcctctcacctggatggctaccacaagctgctgacacaggaaggcatcaccgcctacaacaccctgctgggcggaatctctggcgaggccggcagcagaaagatccagggcatcaatgaactgatcaacagccaccacaaccagcactgccacaagagcgagcggattgccaagctgcggcccctgcacaagcagattctgagcgacggaatgggcgtgtccttcctgcccagcaagttcgccgacgacagcgaagtgtgccaggccgtgaatgagttctaccggcactacgccgacgtgttcgccaaggtgcagagcctgttcgacggcttcgacgactatcagaaagacggcatctacgtggagtacaagaacctgaacgagctgtccaagcaggccttcggcgacttcgctctgctgggaagagtgctggacggctactatgtggacgtcgtgaaccccgagttcaacgagagatttgccaaggccaagaccgacaacgccaaagccaagctgacaaaagagaaggacaagttcatcaagggcgtgcactccctggcttctctggaacaggccatcgagcactacaccgccagacacgacgacgagtctgtgcaggccggaaagctgggccagtacttcaagcacggactggccggcgtggacaaccccatccagaagatccacaacaaccactctacaatcaagggcttcctggaaagagagcggcctgccggcgaaagagccctgcccaagatcaagagcgacaagagccccgagatcagacagctgaaagagctgctggacaacgccctgaacgtggcccacttcgccaaactgctgaccaccaagaccacactgcacaatcaggatggcaacttctacggcgagttcggagccctgtatgatgagctggccaagatcgccaccctgtacaacaaagtgcgggactacctgagccagaagcccttcagcaccgagaagtacaagctgaatttcggcaaccctaccctgctgaacggctgggacctgaacaaagagaaagataacttcggcgtgatcctgcagaaggacggctgctactacctggccctgctggataaggcccacaagaaggtgttcgataacgcccccaacaccggcaagtctgtgtatcagaagatgatctataagctgctgcctggccccaacaagatgctgcccaaggtgttcttcgccaagagcaacctggactactacaaccccagcgccgaactgctggacaaatacgcccagggcacacacaagaaaggcgacaacttcaacctgaaggactgccacgccctgatcgatttcttcaaggccggaatcaacaagcaccccgagtggcagcacttcggcttcaagttcagccccaccagcagctaccaggacctgagcgacttctacagagaggtggaaccccagggctaccaagtgaagttcgtggacatcaacgccgactacatcaatgagctggtggaacagggccagctgtacctgtttcagatctacaacaaggactttagccccaaggcccacggcaagcccaacctgcacaccctgtatttcaaggccctgtttagcgaggacaacctcgtgaatcccatctacaaactgaacggggaggccgagatcttctaccggaaggcctccctggacatgaacgagacaaccatccacagagccggcgaggtgctggaaaacaagaaccctgacaaccctaagaaacggcagttcgtgtacgacatcatcaaggacaagcggtacacccaggataagttcatgctgcacgtgcccatcaccatgaacttcggagtgcagggcatgaccatcaaagagttcaacaaaaaagtgaaccagagcatccagcagtacgacgaagtgaacgtgatcggcatcgaccggggcgagcggcatctgctgtatctgaccgtgatcaactccaagggcgagattctggaacagagatccctgaacgacatcaccaccgcctccgccaacggcacccagatgaccaccccttaccacaagatcctggacaagcgcgagatcgagcggctgaacgccagagtgggatggggagagatcgagacaatcaaagaactgaagtccggctacctgtcccacgtggtgcatcagatctcccagctgatgctgaagtacaacgccatcgtggtgctggaagatctgaattttggcttcaagaggggccggttcaaggtggaaaagcagatctaccagaacttcgagaatgccctgatcaagaaactgaaccacctggtgctgaaagacaaggccgacgacgagatcggcagctacaagaacgccctgcagctgactaacaacttcaccgatctgaagagtatcggcaagcagaccggctttctgttctacgtgcccgcctggaataccagcaagatcgaccccgagacaggcttcgtggacctgctgaagcctagatacgagaatatcgcccagagccaggccttcttcggcaagttcgacaagatctgctacaacgccgataggggctacttcgagttccacatcgactacgccaagttcaatgacaaggccaaaaacagccggcagatctggaaaatctgcagccacggcgataagcgctacgtgtacgataagaccgccaaccagaacaagggcgccaccatcggagtgaatgtgaacgatgagctgaagtccctgttcacccgctaccacatcaacgataagcagcccaatctcgtgatggacatctgccagaacaacgacaaagagtttcacaagagcctgatgtatctgctgaaaacactgctggctctgcggtacagcaacgccagctccgacgaggatttcatcctgagccccgtggccaacgacgagggcgtgttcttcaatagcgccctggccgacgatacccagccccagaatgccgatgccaacggcgcctaccacattgccctgaagggactgtggctgctgaacgaactgaagaacagcgacgatctgaacaaagtgaagctggccatcgacaaccagacctggctgaatttcgcccagaacagaaagaggccggcggccacgaagaaggccggccaggccaagaagaagaagggatcctacccatacgatgttccagattacgcttatccctacgacgtgcctgattatgcatacccatatgatgtccccgactatgcctaa
