|Purpose of plasmid||To express constitutively active (GTP-locked) FLAG-tagged RagA in human cells|
Deutsches Krebsforschungszentrum (DKFZ)
|Depositing Scientist||Aurelio Teleman|
|Bacterial Growth Temp||37|
|Selectable Marker||G418 (geneticin)|
Regulation of TORC1 in response to amino acid starvation via lysosomal recruitment of TSC2.
Demetriades C et al. Cell 2014
|MTA and Terms||
MTA (PDF download)
pcDNA3-FLAG-hRagA Q66L (GTP) was a gift from Aurelio Teleman (European Plasmid Repository plasmid #8).
In the References:
Demetriades C et al.. Regulation of TORC1 in response to amino acid starvation via lysosomal recruitment of TSC2.. Cell 2014 doi:10.1016/j.cell.2014.01.024 PubMed 24529380
Sequence: download sequence