EPR Plasmid #890: pMB50
Sequence: download sequence
| Purpose of plasmid | |
| Depositing Lab |
Martin Parniske Ludwig-Maximilians-Universität München (LMU) |
| Depositing Scientist | Martin Bircheneder |
| Antibiotic Resistance | Kanamycin |
| Bacterial Growth Temp | 37 |
| Copy Number | High |
| Selectable Marker | Hygromycin |
| Final expression system: | Plant cells |
| Reference | This plasmid is from an unpublished project. Please acknowledge the depositing scientist. |
| MTA and Terms |
MTA (PDF download) |
How to cite this reagent:
In the Materials & Methods:
pMB50 was a gift from Martin Parniske (European Plasmid Repository plasmid #890).
In the References:
No reference available. (This plasmid is not yet published.)
pMB50 was a gift from Martin Parniske (European Plasmid Repository plasmid #890).
In the References:
No reference available. (This plasmid is not yet published.)
Sequence: download sequence
GTTGGCGGGTGTCGGGGCGCAGCCATGACCCAGTCACGTAGCGATAGCGGAGTGTATACTGGCTTAACTATGCGGCATCAGAGCAGATTGTACTGAGAGTGCACCATATGCGGTGTGAAATACCGCACAGATGCGTAAGGAGAAAATACCGCATCAGGCGCTCTTCCGCTTCCTCGCTCACTGACTCGCTGCGCTCGGTCGTTCGGCTGCGGCGAGCGGTATCAGCTCACTCAAAGGCGGTAATACGGTTATCCACAGAATCAGGGGATAACGCAGGAAAGAACATGTGAGCAAAAGGCCAGCAAAAGGCCAGGAACCGTAAAAAGGCCGCGTTGCTGGCGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCGACGCTCAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCCTGGAAGCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCTTTCTCCCTTCGGGAAGCGTGGCGCTTTCTCATAGCTCACGCTGTAGGTATCTCAGTTCGGTGTAGGTCGTTCGCTCCAAGCTGGGCTGTGTGCACGAACCCCCCGTTCAGCCCGACCGCTGCGCCTTATCCGGTAACTATCGTCTTGAGTCCAACCCGGTAAGACACGACTTATCGCCACTGGCAGCAGCCACTGGTAACAGGATTAGCAGAGCGAGGTATGTAGGCGGTGCTACAGAGTTCTTGAAGTGGTGGCCTAACTACGGCTACACTAGAAGGACAGTATTTGGTATCTGCGCTCTGCTGAAGCCAGTTACCTTCGGAAAAAGAGTTGGTAGCTCTTGATCCGGCAAACAAACCACCGCTGGTAGCGGTGGTTTTTTTGTTTGCAAGCAGCAGATTACGCGCAGAAAAAAAGGATCTCAAGAAGATCCTTTGATCTTTTCTACGGGGTCTGACGCTCATAAAATCATTATTTGCCATCCAGCTGATATCCCCTATAGTGAGTCGTATTACATGGTCATAGCTGTTTCCTGGCAGCTCTGGCCCGTGTCTCAAAATCTCTGATGTTACATTGCACAAGATAAAAATATATCATCATGAACAATAAAACTGTCTGCTTACATAAACAGTAATACAAGGGGTGTTATGAGCCATATTCAACGGGAAACGTCGAGGCCGCGATTAAATTCCAACATGGATGCTGATTTATATGGGTATAAATGGGCTCGCGATAATGTCGGGCAATCAGGTGCGACAATCTATCGCTTGTATGGGAAGCCCGATGCGCCAGAGTTGTTTCTGAAACATGGCAAAGGTAGCGTTGCCAATGATGTTACAGATGAGATGGTCAGACTAAACTGGCTGACGGAATTTATGCCTCTTCCGACCATCAAGCATTTTATCCGTACTCCTGATGATGCATGGTTACTCACCACTGCGATCCCCGGAAAAACAGCATTCCAGGTATTAGAAGAATATCCTGATTCAGGTGAAAATATTGTTGATGCGCTGGCAGTGTTCCTGCGCCGGTTGCATTCGATTCCTGTTTGTAATTGTCCTTTTAACAGCGATCGCGTATTTCGTCTTGCTCAGGCGCAATCACGAATGAATAACGGTTTGGTTGATGCGAGTGATTTTGATGACGAGCGTAATGGCTGGCCTGTTGAACAAGTCTGGAAAGAAATGCATAAACTTTTGCCATTCTCACCGGATTCAGTCGTCACTCATGGTGATTTCTCACTTGATAACCTTATTTTTGACGAGGGGAAATTAATAGGTTGTATTGATGTTGGACGAGTCGGAATCGCAGACCGATACCAGGATCTTGCCATCCTATGGAACTGCCTCGGTGAGTTTTCTCCTTCATTACAGAAACGGCTTTTTCAAAAATATGGTATTGATAATCCTGATATGAATAAATTGCAGTTTCATTTGATGCTCGATGAGTTTTTCTAATCAGAATTGGTTAATTGGTTGTAACACTGGCAGAGCATTACGCTGACTTGACGGGACGGCGCAAGCTCATGACCAAAATCCCTTAACGTGAGTTACGCGTCGTTCCACTGAGCGTCAGACCCCTGCCGGTAACATGAGCAAAGTCTGCCGCCTTACAACGGCTCTCCCGCTGACGCCGTCCCGGACTGATGGGCTGCCTGTATCGAGTGGTGATTTTGTGCCGAGCTGCCGGTCGGGGAGCTGTTGGCTGGCTGGTGGCAGGATATATTGTGGTGTAAACAAATTGACGCTTAGACGGATTACGGGTCTCAGCGGTACGGCGGGATCATGAGCGGAGAATTAAGGGAGTCACGTTATGACCCCCGCCGATGACGCGGGACAAGCCGTTTTACGTTTGGAACTGACAGAACCGCAACGTTGAAGGAGCCACTCAGCCGCGGGTTTCTGGAGTTTAATGAGCTAAGCACATACGTCAGAAACCATTATTGCGCGTTCAAAAGTCGCCTAAGGTCACTATCAGCTAGCAAATATTTCTTGTCAAAAATGCTCCACTGACGTTCCATAAATTCCCCTCGGTATCCAATTAGAGTCTCATATTCACTCTCAATCCAAATAATCTGCACCGGATCTTCTGGAAGCACCATGAAAAAGCCTGAACTCACCGCGACGTCTGTCGAGAAGTTTCTGATCGAAAAGTTCGACAGCGTgTCCGACCTGATGCAGCTCTCGGAGGGCGAAGAATCTCGTGCTTTCAGCTTCGATGTAGGAGGGCGTGGATATGTCCTGCGGGTAAATAGCTGCGCCGATGGTTTCTACAAAGATCGTTATGTTTATCGGCACTTTGCATCGGCCGCGCTCCCGATTCCGGAAGTGCTTGACATTGGGGAGTTTAGCGAGAGCCTGACCTATTGCATCTCCCGCCGTtCACAGGGTGTCACGTTGCAAGACCTGCCTGAAACCGAACTGCCCGCTGTTCTACAACCGGTCGCGGAGGCTATGGATGCGATCGCTGCGGCCGATCTTAGCCAGACGAGCGGGTTCGGCCCATTCGGACCGCAAGGAATCGGTCAATACACTACATGGCGTGATTTCATATGCGCGATTGCTGATCCCCATGTGTATCACTGGCAAACTGTGATGGACGACACCGTCAGTGCGTCCGTCGCGCAGGCTCTCGATGAGCTGATGCTTTGGGCCGAGGACTGCCCCGAAGTCCGGCACCTCGTGCACGCGGATTTCGGCTCCAACAATGTCCTGACGGACAATGGCCGCATAACAGCGGTCATTGACTGGAGCGAGGCGATGTTCGGGGATTCCCAATACGAGGTCGCCAACATCTTCTTCTGGAGGCCGTGGTTGGCTTGTATGGAGCAGCAGACGCGCTACTTCGAGCGGAGGCATCCGGAGCTTGCAGGATCGCCACGACTCCGGGCGTATATGCTCCGCATTGGTCTTGACCAACTCTATCAGAGCTTGGTTGACGGCAATTTCGATGATGCAGCTTGGGCGCAGGGTCGATGCGACGCAATCGTCCGATCCGGAGCCGGGACTGTCGGGCGTACACAAATCGCCCGCAGAAGCGCGGCCGTCTGGACCGATGGCTGTGTAGAAGTACTCGCCGATAGTGGAAACCGACGCCCCAGCACTCGTCCGAGGGCAAAGAAATAGAAGGGCTAAGACCTAAGCTAGCCTAGAATCCGGCCATGCTAGAGTCCGCAAAAATCACCAGTCTCTCTCTACAAATCTATCTCTCTCTATTTTTCTCCAGAATAATGTGTGAGTAGTTCCCAGATAAGGGAATTAGGGTTCTTATAGGGTTTCGCTCATGTGTTGAGCATATAAGAAACCCTTAGTATGTATTTGTATTTGTAAAATACTTCTATCAATAAAATTTCTAATTCCTAAAACCAAAATCCAGTGACCTTGAGTGACCTAAGCTAGCCTAGTGTCGGGCGCGGGGAGAGAGGATTTTGAGGAAATAATTAATTGAATTACTTGTATTATTGATAAAGTAATTTAGATAAGTTGTTAGTACAACTTATTGCAACTATGGATAGACAAAAATCACTTATTTTAAGGGGAGAAGTATATAACAACTTATTATAAAATTTCTGAGCAGCCGGCCTCCTCCAATCAATCATAGAGAGTGGAGCCCATTCTGGAAAACCAAGGAACCCCCAACTTGCACTGGTGCGGTGGCCCAATTCAAAAAAAACGGGGCCCAGCAAAGTAACGCCGTTGTAACGATTTATCAATCCAAACTCAAAAGGCGGCGAGATGCGTTTAACTCCGTGAAATTAACAAACCGCCAACAACTTGCAATTTGCAACTACCGTTTCCAAGAAGAACTCAACCACACAACGTATCCTATCCCAAACCACACGCAACTAGTGACGCGTCATAAGGACACGTGTCACAATTTGACTGGTTAATAATTTCACCGCTTTTGCTATAAATTACCTCCAATCCCCTTAGCTTCTTCACAATTCAGTTCCCAACCCTAACAATTCTTGTTCATATCGCTTCTCTCTACTTTCAAGGTATGATCCAATTTCTCTCTTCTTTCTCTGTAATCCTTTCGTTGAGTTTTGTTTCCGATCAATCATAGGTAGTTTTCTTGTTTCGAAGCATGAGATCTAGGAATTTTTTGTGATTTTCCAAAATTGAGATCGGTTTGAAATTGAATTTTACAGCTTGAATCTCAGATCTTGTTTTATCAATGTTTTCGATGGCTTGCGATGTAGATCTATGATAATTGTGGTTCAGTTTTGTTAGGAATCGATTTCGGTTTAGCAATTGCAGATTAATTAGGGTTTCCAATTGAATTCTTCAGATCCGTTATGGAATTATGTCAAATAATTTATTCAAATTGGAAATTATTGTTAGATCCACTCTTAATCTGTTTGATCCAAGCTTCAATTAGGGTTTTCACTTGTTTCAATTTCTTGTTATGGATTCTGATTTATCTGTTGATGTTAGATCCACTCTTAATCTGTTTGATCCAAGCGTTAATTAGGGTTTTCACTTGTTTCAATTTCGTGTGTTGGATTCTGATTTATCTGTTGTTGATGTGATTACAGTCTGAACAATGCTGCAGCCTAAGAAGAAGAGAAAGGTTGGAGGAGTCGACTCGAGTGCGGCCGCCACCATGAGCAAGCTCGAGAAGTTTACCAACTGCTACAGCCTCTCTAAGACCCTCAGGTTCAAGGCTATCCCTGTGGGAAAGACCCAAGAGAATATCGACAACAAGAGGCTCCTCGTCGAGGATGAGAAGAGAGCTGAAGATTACAAGGGCGTGAAGAAGCTCCTCGACAGGTACTACCTCAGCTTCATCAACGATGTGCTCCACAGCATCAAGCTCAAGAACCTCAACAACTACATCAGCCTCTTCCGTAAGAAAACCAGGACCGAGAAAGAGAACAAAGAGCTTGAGAACCTCGAGATCAACCTCCGTAAAGAGATCGCCAAGGCTTTCAAGGGAAACGAGGGATACAAGAGCCTCTTCAAGAAGGATATTATCGAGACAATCCTGCCTGAGTTCCTGGACGATAAGGATGAGATCGCTCTCGTGAACAGCTTCAACGGATTCACTACTGCCTTCACCGGATTCTTCAGAAACAGGGAAAACATGTTCAGCGAAGAGGCCAAGAGCACCTCTATCGCTTTCAGATGCATCAACGAGAACCTCACGCGTTACATCAGCAACATGGACATCTTCGAGAAGGTGGACGCCATCTTCGATAAGCACGAGGTGCAAGAAATCAAAGAGAAGATCCTCAACAGCGACTACGACGTCGAGGACTTTTTTGAAGGGGAGTTCTTCAACTTCGTTCTCACCCAAGAGGGCATCGACGTGTACAACGCTATTATCGGAGGATTCGTGACCGAGTCTGGGGAGAAGATTAAGGGACTCAACGAGTACATCAACCTGTACAACCAGAAAACGAAGCAGAAGCTCCCGAAGTTCAAGCCGCTCTACAAGCAGGTTCTCTCTGATCGTGAGAGCCTCTCATTTTACGGTGAGGGTTACACCTCTGACGAGGAAGTGCTTGAGGTTTTCCGTAACACCCTCAACAAGAACAGCGAGATCTTCTCGTCCATCAAGAAGTTGGAGAAGCTTTTCAAGAACTTCGACGAGTACAGCAGCGCTGGGATCTTCGTTAAGAACGGACCTGCTATCAGCACCATCAGCAAGGATATTTTCGGCGAGTGGAACGTGATCAGGGACAAGTGGAATGCTGAGTACGATGACATCCACCTCAAGAAGAAGGCTGTCGTCACTGAGAAGTACGAGGATGACAGGCGTAAGTCGTTCAAGAAGATCGGCTCTTTCAGCCTCGAGCAGCTTCAAGAATACGCTGATGCTGATCTCAGCGTGGTCGAGAAGCTCAAAGAGATCATCATCCAGAAGGTCGACGAGATCTACAAGGTGTACGGGTCCTCTGAGAAGTTGTTCGATGCTGATTTCGTCCTCGAGAAGAGTCTGAAGAAGAACGACGCTGTCGTCGCGATCATGAAGGATTTGCTCGACAGCGTGAAGTCCTTCGAGAACTATATCAAGGCCTTCTTCGGAGAGGGCAAAGAGACTAATAGGGACGAGTCTTTCTACGGGGATTTCGTGCTCGCTTACGATATCCTCCTCAAGGTGGACCATATCTACGACGCCATCAGAAACTACGTGACCCAGAAGCCTTACAGCAAGGACAAGTTCAAGTTGTACTTTCAGAACCCGCAGTTCATGGGCGGATGGGACAAAGACAAAGAGACAGATTACAGGGCCACCATCCTCAGGTACGGGTCTAAGTACTACCTGGCCATCATGGACAAGAAATACGCCAAGTGCCTCCAAAAGATCGACAAGGATGACGTGAACGGGAACTATGAGAAGATCAACTACAAGCTCCTTCCGGGACCGAACAAGATGCTTCCTAAGGTGTTCTTCAGCAAGAAATGGATGGCCTACTACAACCCGTCTGAGGACATCCAGAAAATCTACAAGAACGGGACCTTCAAGAAAGGCGACATGTTCAACCTCAACGACTGCCACAAGCTCATCGATTTCTTCAAGGACAGCATCTCGCGTTACCCGAAGTGGTCTAACGCTTACGACTTTAACTTCAGCGAGACAGAAAAGTACAAGGATATCGCCGGGTTCTACCGTGAGGTTGAGGAACAGGGTTACAAGGTTAGCTTCGAGAGCGCCTCCAAGAAAGAGGTTGACAAGTTGGTCGAAGAGGGCAAGCTCTACATGTTCCAGATCTATAACAAGGACTTCTCCGACAAGAGCCACGGAACTCCTAACCTCCATACGATGTACTTCAAGCTGCTTTTCGACGAGAACAACCACGGGCAGATCAGACTTTCTGGTGGTGCTGAACTCTTCATGCGTAGGGCCTCACTCAAGAAAGAAGAGTTGGTTGTTCACCCGGCCAACTCTCCAATCGCTAACAAGAATCCTGACAACCCGAAAAAGACCACCACGCTGTCTTACGACGTCTACAAGGACAAAAGGTTCAGCGAGGACCAGTACGAGCTTCATATCCCGATCGCTATCAACAAGTGCCCGAAGAACATCTTCAAGATCAATACCGAGGTGAGGGTGCTGCTCAAGCACGATGATAACCCTTACGTGATCGGAATCGATCGTGGTGAGAGAAACCTCCTCTACATCGTTGTGGTGGACGGAAAGGGAAACATCGTCGAGCAGTACAGCCTGAACGAGATTATCAACAATTTCAACGGCATCAGGATCAAGACCGACTACCACTCACTCCTCGATAAGAAAGAAAAAGAGCGTTTCGAGGCCAGGCAGAACTGGACTTCTATCGAAAACATCAAAGAGTTGAAGGCCGGCTACATCTCTCAGGTGGTGCATAAGATCTGCGAGCTGGTGGAAAAGTACGATGCTGTGATCGCTCTTGAGGACCTCAACTCTGGGTTCAAGAACAGTAGAGTGAAGGTTGAGAAGCAGGTCTACCAAAAGTTCGAGAAGATGCTCATCGACAAGCTCAACTACATGGTGGACAAAAAGAGCAACCCTTGCGCTACCGGTGGTGCTCTTAAGGGATACCAGATCACGAACAAGTTCGAGTCCTTCAAGAGCATGAGCACCCAGAACGGCTTCATCTTCTATATCCCTGCTTGGCTCACCAGCAAGATCGATCCTTCTACTGGTTTCGTGAACCTGCTCAAGACCAAGTACACCTCGATCGCCGACAGCAAGAAGTTCATCTCGTCTTTCGACAGGATCATGTACGTGCCGGAAGAGGATCTTTTCGAGTTCGCTCTCGACTATAAGAACTTCAGCAGGACCGACGCCGACTACATTAAGAAGTGGAAGCTCTACTCCTACGGGAACCGTATCAGGATCTTCCGAAATCCGAAGAAAAACAACGTGTTCGACTGGGAAGAAGTGTGCCTCACCTCTGCCTACAAAGAACTGTTCAACAAGTACGGCATCAACTACCAGCAGGGTGATATCAGGGCTCTTTTGTGCGAGCAGAGCGACAAGGCATTCTACAGCTCATTCATGGCCCTCATGTCTCTCATGCTCCAGATGAGGAACTCTATCACCGGAAGGACCGATGTGGACTTCCTTATCTCTCCGGTCAAGAACTCTGACGGGATCTTCTACGACAGCCGTAACTATGAGGCTCAAGAGAACGCTATCCTGCCGAAGAATGCTGATGCAAACGGGGCTTACAACATTGCGAGAAAGGTTCTCTGGGCTATCGGGCAGTTTAAGAAAGCGGAAGATGAGAAGCTGGACAAGGTGAAGATCGCCATCTCCAACAAAGAGTGGCTTGAGTACGCTCAGACCTCCGTTAAGCACAAGAGGCCTGCTGCTACTAAGAAAGCTGGTCAGGCTAAGAAGAAGAAATGAAAGGGCTAAGACCTAAGCTAGCCTAGAATCatacagcattcccagaaagagaaacagaagaaatatacaaactttcattttgagagcagcacctcgtctattgattgcagataatatgcttctcatttgtatttccttttgattatttttgtttctatccctttgtttgagtcaatctcaaatattcggtcattgttggtatgaaaaatcaagcagttcatgttaagagtcaatttaaaattaatatttttatatagagttgtatgtgaaatgatgttgtgatttggtatatatggataaagagcttgtcagttcattttGCtctcatttttttggtatccaaataagaaacacaaaagggatatgtccctctactatcaaatattagttataagtattcatgttatactattcgatattttctaccccaatcgttacctatttaaaagtatttacccctccatctatcaaacccctggacccagctttcctattacatgtggcttcatcttaagcccccaaacctttttcttatttttgatttttaaaggctcatcttaaaatttattactcaaattaatacctcttaataacccacctcaaggacccagtaattaaatatccaattagctccagtaattggggttcatattagctccagtcttaaattttaaaggcgatgatcgtattcctccacttggttcatttatactcaaagaatactcaatgtctttagtgtttagataactttttgtaaatcatatagattgttttaacaaaaaacaattcaatagtagattttcacatgaaagttacataaaaattctttaaaattactttctcaaaaaattgttccaaacatattatcccacaattaaactcaatctgtttttcgaaacctaaatcaaaaccaatccaactaccttatataatatataatcaatacattgtaaagaactgcatgttcttttaaattttgggggcaaagttattccgtacgttcacacatgtactaataggaggtaataaatgatatgtgaaacaatcgaggtgtaaacaagctagcatTGAGCAGGCCTCCCAGCTTTCGTCCGTATCATCGGTTTCGACAACGTTCGTCAAGTTCAATGCATCAGTTTCATTGCCCACACACCAGAATCCTACTAAGTTTGAGTATTATGGCATTGGAAAAGCTGTTTTCTTCTATCATTTGTTCTGCTTGTAATTTACTGTGTTCTTTCAGTTTTTGTTTTCGGACATCAAAATGCAAATGGATGGATAAGAGTTAATAAATGATATGGTCCTTTTGTTCATTCTCAAATTATTATTATCTGTTGTTTTTACTTTAATGGGTTGAATTTAAGTAAGAAAGGAACTAACAGTGTGATATTAAGGTGCAATGTTAGACATATAAAACAGTCTTTCACCTCTCTTTGGTTATGTCTTGAATTGGTTTGTTTCTTCACTTATCTGTGTAATCAAGTTTACTATGAGTCTATGATCAAGTAATTATGCAATCAAGTTAAGTACAGTATAGGCTTTGTCATTGGCGGCTTTTTTTCTTCTTCTTCGTTCATACAGTTTTTTTTTGTTTATCAGCTTACATTTTCTTGAACCGTAGCTTTCGTTTTCTTCTTTTTAACTTTCCATTCGGAGTTTTTGTATCTTGTTTCATAGTTTGTCCCAGGATTAGAATGATTAGGCATCGAACCTTCAAGAATTTGATTGAATAAAACATCTTCATTCTTAAGATATGAAGATAATCTTCAAAAGGCCCCTGGGAATCTGAAAGAAGAGAAGCAGGCCCATTTATATGGGAAAGAACAATAGTATTTCTTATATAGGCCCATTTAAGTTGAAAACAATCTTCAAAAGTCCCACATCGCTTAGATAAGAAAACGAAGCTGAGTTTATATACAGCTAGAGTCGAAGTAGTGATTGAAATTACTGATGAGTCCGTGAGGACGAAACGAGTAAGCTCGTCTAATTTCTACTAAGTGTAGATAGGGGGACTATGTGAGTCGTGGCCGGCATGGTCCCAGCCTCCTCGCTGGCGCCGGCTGGGCAACATGCTTCGGCATGGCGAATGGGACTTTTTTGACGCTAGCCTAGTTCGCTTTTTTTCTTCTTCTTCGTTCATACAGTTTTTTTTTGTTTATCAGCTTACATTTTCTTGAACCGTAGCTTTCGTTTTCTTCTTTTTAACTTTCCATTCGGAGTTTTTGTATCTTGTTTCATAGTTTGTCCCAGGATTAGAATGATTAGGCATCGAACCTTCAAGAATTTGATTGAATAAAACATCTTCATTCTTAAGATATGAAGATAATCTTCAAAAGGCCCCTGGGAATCTGAAAGAAGAGAAGCAGGCCCATTTATATGGGAAAGAACAATAGTATTTCTTATATAGGCCCATTTAAGTTGAAAACAATCTTCAAAAGTCCCACATCGCTTAGATAAGAAAACGAAGCTGAGTTTATATACAGCTAGAGTCGAAGTAGTGATTGAAATTACTGATGAGTCCGTGAGGACGAAACGAGTAAGCTCGTCTAATTTCTACTAAGTGTAGATATGAGTACCGGAATCACCAGGGCCGGCATGGTCCCAGCCTCCTCGCTGGCGCCGGCTGGGCAACATGCTTCGGCATGGCGAATGGGACTTTTTTGTCTGTCGCGGGGAGAGAGGATTTTGAGGAAATAATTAATTGAATTACTTGTATTATTGATAAAGTAATTTAGATAAGTTGTTAGTACAACTTATTGCAACTATGGATAGACAAAAATCACTTATTTTAAGGGGAGAAGTATATAACAACTTATTATAAAATTTCTGAGCAGCCGGCCTCCTCCAATCAATCATAGAGAGTGGAGCCCATTCTGGAAAACCAAGGAACCCCCAACTTGCACTGGTGCGGTGGCCCAATTCAAAAAAAACGGGGCCCAGCAAAGTAACGCCGTTGTAACGATTTATCAATCCAAACTCAAAAGGCGGCGAGATGCGTTTAACTCCGTGAAATTAACAAACCGCCAACAACTTGCAATTTGCAACTACCGTTTCCAAGAAGAACTCAACCACACAACGTATCCTATCCCAAACCACACGCAACTAGTGACGCGTCATAAGGACACGTGTCACAATTTGACTGGTTAATAATTTCACCGCTTTTGCTATAAATTACCTCCAATCCCCTTAGCTTCTTCACAATTCAGTTCCCAACCCTAACAATTCTTGTTCATATCGCTTCTCTCTACTTTCAAGGTATGATCCAATTTCTCTCTTCTTTCTCTGTAATCCTTTCGTTGAGTTTTGTTTCCGATCAATCATAGGTAGTTTTCTTGTTTCGAAGCATGAGATCTAGGAATTTTTTGTGATTTTCCAAAATTGAGATCGGTTTGAAATTGAATTTTACAGCTTGAATCTCAGATCTTGTTTTATCAATGTTTTCGATGGCTTGCGATGTAGATCTATGATAATTGTGGTTCAGTTTTGTTAGGAATCGATTTCGGTTTAGCAATTGCAGATTAATTAGGGTTTCCAATTGAATTCTTCAGATCCGTTATGGAATTATGTCAAATAATTTATTCAAATTGGAAATTATTGTTAGATCCACTCTTAATCTGTTTGATCCAAGCTTCAATTAGGGTTTTCACTTGTTTCAATTTCTTGTTATGGATTCTGATTTATCTGTTGATGTTAGATCCACTCTTAATCTGTTTGATCCAAGCGTTAATTAGGGTTTTCACTTGTTTCAATTTCGTGTGTTGGATTCTGATTTATCTGTTGTTGATGTGATTACAGTCTGAACAATGGGCAGCCACCACCACCACCACCACAGCGGAATGAGTTCACTGCCGTATAGGCAGCTAAGAAAATCAGGTGGCACCGGCGGCAGCGGCGGATCCATGGTGAGCAAGGGCGAGGAGCTGTTCACCGGGGTGGTGCCCATCCTGGTCGAGCTGGACGGCGACGTAAACGGCCACAAGTTCAGCGTGTCCGGCGAGGGCGAGGGCGATGCCACCTACGGCAAGCTGACCCTGAAGTTCATCTGCACCACCGGCAAGCTGCCCGTGCCCTGGCCCACCCTCGTGACCACCTTCGGCTACGGCCTGATGTGCTTCGCCCGCTACCCCGACCACATGAAGCAGCACGACTTCTTCAAGTCCGCCATGCCCGAAGGCTACGTCCAGGAGCGCACCATCTTCTTCAAGGACGACGGCAACTACAAGACCCGCGCCGAGGTGAAGTTCGAGGGCGACACCCTGGTGAACCGCATCGAGCTGAAGGGCATCGACTTCAAGGAGGACGGCAACATCCTGGGGCACAAGCTGGAGTACAACTACAACAGCCACAACGTCTATATCATGGCCGACAAGCAGAAGAACGGCATCAAGGTGAACTTCAAGATCCGCCACAACATCGAGGACGGCAGCGTGCAGCTCGCCGACCACTACCAGCAGAACACCCCCATCGGCGACGGCCCCGTGCTGCTGCCCGACAACCACTACCTGAGCTACCAGTCCAAGCTGAGCAAAGACCCCAACGAGAAGCGCGATCACATGGTCCTGCTGGAGTTCGTGACCGCCGCCGGGATCACTCTCGGCATGGACGAGCTGTACAAGAAGGGACTGCAGCCTAAGAAGAAGAGAAAGGTTGGAGGAGTCGACTCGAGTGCGTAAAATCGATCGTTCAAACATTTGGCAATAAAGTTTCTTAAGATTGAATCCTGTTGCCGGTCTTGCGATGATTATCATATAATTTCTGTTGAATTACGTTAAGCATGTAATAATTAACATGTAATGCATGACGTTATTTATGAGATGGGTTTTTATGATTAGAGTCCCGCAATTATACATTTAATACGCGATAGAAAACAAAATATAGCGCGCAAACTAGGATAAATTATCGCGCGCGGTGTCATCTATGTTACTAGATCTGAGTGACCTAAGCTAGCCTAGTGTCTGAGGCGGCCAACAAACTATTCGTCACGTCGACAAAATTTAGAACGAACTTAATTATGATCTCAAATACATTGATACATATCTCATCTAGATCTAGGTTATCATTATGTAAGAAAGTTTTGACGAATATGGCACGACAAAATGGCTAGACTCGATGTAATTGGTATCTCAACTCAACATTATACTTATACCAAACATTAGTTAGACAAAATTTAAACAACTATTTTTTATGTATGCAAGAGTCAGCATATGTATAATTGATTCAGAATCGTTTTGACGAGTTCGGATGTAGTAGTAGCCATTATTTAATGTACATACTAATCGTGAATAGTGAATATGATGAAACATTGTATCTTATTGTATAAATATCCATAAACACATCATGAAAGACACTTTCTTTCACGGTCTGAATTAATTATGATACAATTCTAATAGAAAACGAATTAAATTACGTTGAATTGTATGAAATCTAATTGAACAAGCCAACCACGACGACGACTAACGTTGCCTGGATTGACTCGGTTTAAGTTAACCACTAAAAAAACGGAGCTGTCATGTAACACGCGGATCGAGCAGGTCACAGTCATGAAGCCATCAAAGCAAAAGAACTAATCCAAGGGCTGAGATGATTAATTAGTTTAAAAATTAGTTAACACGAGGGAAAAGGCTGTCTGACAGCCAGGTCACGTTATCTTTACCTGTGGTCGAAATGATTCGTGTCTGTCGATTTTAATTATTTTTTTGAAAGGCCGAAAATAAAGTTGTAAGAGATAAACCCGCCTATATAAATTCATATATTTTCCTCTCCGCTTTGAATTGTCTCGTTGTCCTCCTCACTTTCATCAGCCGTTTTGAATCTCCGGCGACTTGACAGAGAAGAACAAGGAAGAAGtCTAAGAGAGAAAGTAAGAGATAATCCAGGAGATTCATTCTCCGTTTTGAATCTTCCTCAATCTCATCTTCTTCCGCTCTTTCTTTCCAAGGTAATAGGAACTTTCTGGATCTACTTTATTTGCTGGATCTCGATCTTGTTTTCTCAATTTCCTTGAGATCTGGAATTCGTTTAATTTGGATCTGTGAACCTCCACTAAATCTTTTGGTTTTACTAGAATCGATCTAAGTTGACCGATCAGTTAGCTCGATTATAGCTACCAGAATTTGGCTTGACCTTGATGGAGAGATCCATGTTCATGTTACCTGGGAAATGATTTGTATATGTGAATTGAAATCTGAACTGTTGAAGTTAGATTGAATCTGAACACTGTCAATGTTAGATTGAATCTGAACACTGTTTAAGGTTAGATGAAGTTTGTGTATAGATTCTTCGAAACTTTAGGATTTGTAGTGTCGTACGTTGAACAGAAAGCTATTTCTGATTCAATCAGGGTTTATTTGACTGTATTGAACTCTTTTTGTGTGTTTGCAGCTCATAAAAATCTGCCCACGACTCACATAGTCCCCCTCAAACACCATGGATCATTATCTTGATATTAGACTTAGACCTGATCCTGAATTTCCTCCTGCTCAACTTATGTCTGTTCTTTTTGGAAAGCTTCATCAAGCTCTTGTTGCTCAAGGAGGAGATAGAATTGGAGTTTCTTTTCCTGATCTTGATGAATCTAGATCTAGACTTGGAGAAAGACTTAGAATTCATGCTTCTGCTGATGATCTTAGAGCTCTTCTTGCTAGACCTTGGCTTGAAGGACTTAGAGATCATCTTCAATTTGGAGAACCTGCTGTTGTTCCTCATCCTACTCCTTATAGACAAGTTTCTAGAGTTCAAGCTAAGTCTAATCCTGAAAGACTTAGAAGAAGATTGATGAGGAGACATGATCTTTCTGAAGAAGAAGCTAGAAAGAGAATTCCTGATACTGTTGCTAGAGCTCTTGATCTTCCTTTTGTTACTCTTAGATCTCAATCTACTGGACAACATTTTAGACTTTTTATTAGACATGGACCTCTTCAAGTTACTGCTGAAGAAGGAGGATTTACTTGTTATGGACTTTCTAAGGGAGGATTTGTTCCTTGGTTTTGAAAGGTTTGATGAGTACCGGAATCACCAGGGGAATCATATGAAGATGAAGATGAAATATTTGGTGTGTCAAATAAAAAGCTTGTGTGCTTAAGTTTGTGTTTTTTTCTTGGCTTGTTGTGTTATGAATTTGTGGCTTTTTCTAATATTAAATGAATGTAAGATCTCATTATAATGAATAAACAAATGTTTCTATAATCCATTGTGAATGTTTTGTTGGATCTCTTCTGCAGCATATAACTACTGTATGTGCTATGGTATGGACTATGGAATATGATTAAAGATAAGTGAGTGACCTAAGCTAGCCTAGTGTCTCTGTCTGTGAGACCTCTGGTTTAAACTATCAGTGTTTGACAGGATATATTGGCGGGTAAACCTAAGAGAAAAGAGCGTTTATTAGAATAAtCGGATATTTAAAAGGGCGTGAAAAGGTTTATCCGTTCGTCCATTTGTATGTGCATGCCAACCACAGGGTTCCCCTCGGGATCAAAGTACTTTGATCCAACCCCTCCGCTGCTATAGTGCAGTCGGCTTCTGACGTTCAGTGCAGCCTTCTTCTGAAAACGACATGTCGCACAAGTCCTAAGTTACGCGACAGGCTGCCGCCCTGCCCTTTTCCTGGCGTTTTCTTGTCGCGTGTTTTAGTCGCATAAAGTAGAATACTTGCGACTAGAACCGGAGACATTACGCCATGAACAAGAGCGCCGCCGCTGGCCTGCTGGGCTATGCCCGCGTCAGCACCGACGACCAGGACTTGACCAACCAACGGGCCGAACTGCACGCGGCCGGCTGCACCAAGCTGTTTTCCGAGAAGATCACCGGCACCAGGCGCGACCGCCCGGAGCTGGCCAGGATGCTTGACCACCTACGCCCTGGCGACGTTGTGACAGTGACCAGGCTAGACCGCCTGGCCCGCAGCACCCGCGACCTACTGGACATTGCCGAGCGCATCCAGGAGGCCGGCGCGGGCCTGCGTAGCCTGGCAGAGCCGTGGGCCGACACCACCACGCCGGCCGGCCGCATGGTGTTGACCGTGTTCGCCGGCATTGCCGAGTTCGAGCGTTCCCTAATCATCGACCGCACCCGGAGCGGGCGCGAGGCCGCCAAGGCCCGAGGCGTGAAGTTTGGCCCCCGCCCTACCCTCACCCCGGCACAGATCGCGCACGCCCGCGAGCTGATCGACCAGGAAGGCCGCACCGTGAAAGAGGCGGCTGCACTGCTTGGCGTGCATCGCTCGACCCTGTACCGCGCACTTGAGCGCAGCGAGGAAGTGACGCCCACCGAGGCCAGGCGGCGCGGTGCCTTCCGTGAGGACGCATTGACCGAGGCCGACGCCCTGGCGGCCGCCGAGAATGAACGCCAAGAGGAACAAGCATGAAACCGCACCAGGACGGCCAGGACGAACCGTTTTTCATTACCGAAGAGATCGAGGCGGAGATGATCGCGGCCGGGTACGTGTTCGAGCCGCCCGCGCACATCTCAACCGTGCGGCTGCATGAAATCCTGGCCGGTTTGTCTGATGCCAAGCTGGCGGCCTGGCCGGCCAGCTTGGCCGCTGAAGAAACCGAGCGCCGCCGTCTAAAAAGGTGATGTGTATTTGAGTAAAACAGCTTGCGTCATGCGGTCGCTGCGTATATGATGCGATGAGTAAATAAACAAATACGCAAGGGGAACGCATGAAGGTTATCGCTGTACTTAACCAGAAAGGCGGGTCAGGCAAGACGACCATCGCAACCCATCTAGCCCGCGCCCTGCAACTCGCCGGGGCCGATGTTCTGTTAGTCGATTCCGATCCCCAGGGCAGTGCCCGCGATTGGGCGGCCGTGCGGGAAGATCAACCGCTAACCGTTGTCGGCATCGACCGCCCGACGATTGACCGCGACGTGAAGGCCATCGGCCGGCGCGACTTCGTAGTGATCGACGGAGCGCCCCAGGCGGCGGACTTGGCTGTGTCCGCGATCAAGGCAGCCGACTTCGTGCTGATTCCGGTGCAGCCAAGCCCTTACGACATATGGGCCACCGCCGACCTGGTGGAGCTGGTTAAGCAGCGCATTGAGGTCACGGATGGAAGGCTACAAGCGGCCTTTGTCGTGTCGCGGGCGATCAAAGGCACGCGCATCGGCGGTGAGGTTGCCGAGGCGCTGGCCGGGTACGAGCTGCCCATTCTTGAGTCCCGTATCACGCAGCGCGTGAGCTACCCAGGCACTGCCGCCGCCGGCACAACCGTTCTTGAATCAGAACCCGAGGGCGACGCTGCCCGCGAGGTCCAGGCGCTGGCCGCTGAAATTAAATCAAAACTCATTTGAGTTAATGAGGTAAAGAGAAAATGAGCAAAAGCACAAACACGCTAAGTGCCGGCCGTCCGAGCGCACGCAGCAGCAAGGCTGCAACGTTGGCCAGCCTGGCAGACACGCCAGCCATGAAGCGGGTCAACTTTCAGTTGCCGGCGGAGGATCACACCAAGCTGAAGATGTACGCGGTACGCCAAGGCAAGACCATTACCGAGCTGCTATCTGAATACATCGCGCAGCTACCAGAGTAAATGAGCAAATGAATAAATGAGTAGATGAATTTTAGCGGCTAAAGGAGGCGGCATGGAAAATCAAGAACAACCAGGCACCGACGCCGTGGAATGCCCCATGTGTGGAGGAACGGGCGGTTGGCCAGGCGTAAGCGGCTGGGTTGcCTGCCGGCCCTGCAATGGCACTGGAACCCCCAAGCCCGAGGAATCGGCGTGAgCGGTCGCAAACCATCCGGCCCGGTACAAATCGGCGCGGCGCTGGGTGATGACCTGGTGGAGAAGTTGAAGGCCGCGCAGGCCGCCCAGCGGCAACGCATCGAGGCAGAAGCACGCCCCGGTGAATCGTGGCAAGCGGCCGCTGATCGAATCCGCAAAGAATCCCGGCAACCGCCGGCAGCCGGTGCGCCGTCGATTAGGAAGCCGCCCAAGGGCGACGAGCAACCAGATTTTTTCGTTCCGATGCTCTATGACGTGGGCACCCGCGATAGTCGCAGCATCATGGACGTGGCCGTTTTCCGTCTGTCGAAGCGTGACCGACGAGCTGGCGAGGTGATCCGCTACGAGCTTCCAGACGGGCACGTAGAGGTTTCCGCAGGGCCGGCCGGCATGGCCAGTGTGTGGGATTACGACCTGGTACTGATGGCGGTTTCCCATCTAACCGAATCCATGAACCGATACCGGGAAGGGAAGGGAGACAAGCCCGGCCGCGTGTTCCGTCCACACGTTGCGGACGTACTCAAGTTCTGCCGGCGAGCCGATGGCGGAAAGCAGAAAGACGACCTGGTAGAAACCTGCATTCGGTTAAACACCACGCACGTTGCCATGCAGCGTACGAAGAAGGCCAAGAACGGCCGCCTGGTGACGGTATCCGAGGGTGAAGCCTTGATTAGCCGCTACAAGATCGTAAAGAGCGAAACCGGGCGGCCGGAGTACATCGAGATCGAGCTAGCTGATTGGATGTACCGCGAGATCACAGAAGGCAAGAACCCGGACGTGCTGACGGTTCACCCCGATTACTTTTTGATCGATCCCGGCATCGGCCGTTTTCTCTACCGCCTGGCACGCCGCGCCGCAGGCAAGGCAGAAGCCAGATGGTTGTTCAAGACGATCTACGAACGCAGTGGCAGCGCCGGAGAGTTCAAGAAGTTCTGTTTCACCGTGCGCAAGCTGATCGGGTCAAATGACCTGCCGGAGTACGATTTGAAGGAGGAGGCGGGGCAGGCTGGCCCGATCCTAGTCATGCGCTACCGCAACCTGATCGAGGGCGAAGCATCCGCCGGTTCCTAATGTACGGAGCAGATGCTAGGGCAAATTGCCCTAGCAGGGGAAAAAGGTCGAAAAGGACTCTTTCCTGTGGATAGCACGTACATTGGGAACCCAAAGCCGTACATTGGGAACCGGAACCCGTACATTGGGAACCCAAAGCCGTACATTGGGAACCGGTCACACATGTAAGTGACTGATATAAAAGAGAAAAAAGGCGATTTTTCCGCCTAAAACTCTTTAAAACTTATTAAAACTCTTAAAACCCGCCTGGCCTGTGCATAACTGTCTGGCCAGCGCACAGCCGAAGAGCTGCAAAAAGCGCCTACCCTTCGGTCGCTGCGCTCCCTACGCCCCGCCGCTTCGCGTCGGCCTATCGCGGCCGCTGGCCGCTCAAAAATGGCTGGCCTACGGCCAGGCAATCTACCAGGGCGCGGACAAGCCGCGCCGTCGCCACTCGACCGCCGGCGCCCACATCAAGGCACCCTGCCTCGCGCGTTTCGGTGATGACGGTGAAAACCTCTGACACATGCAGCTCCCGGAGACAGTCACAGCTTGTCTGTAAGCGGATGCCGGGAGCAGACAAGCCCGTCAGGGCGCGTCAGCGGGT
next plasmid