EPR Plasmid #944: pNH605-Padh-AtAFB2(F74A)
Plasmid Details
| Purpose | |
| Depositing Lab |
Sebastian Schuck Heidelberg University |
| Depositing Scientist | Sebastian Schuck |
| Antibiotic Resistance | Ampicillin |
| Bacterial Growth Temp | 37 |
| Copy Number | High |
| Selectable Marker | |
| Reference |
An optimised system for rapid auxin-inducible protein degradation in budding yeast Schuck, S.; Hubbe, P.; Sharma, C.; Pajonk, O.; Peters, N.; Guschtschin-Schmidt, N.; Friemel, N. DOI See other plasmids from this preprint |
| MTA and Terms |
MTA (PDF download) |
How to Cite This Reagent
IN THE MATERIALS & METHODS:
pNH605-Padh-AtAFB2(F74A) was a gift from Sebastian Schuck (European Plasmid Repository plasmid #944).
pNH605-Padh-AtAFB2(F74A) was a gift from Sebastian Schuck (European Plasmid Repository plasmid #944).
IN THE REFERENCES:
Schuck, S.; Hubbe, P.; Sharma, C.; Pajonk, O.; Peters, N.; Guschtschin-Schmidt, N.; Friemel, N. . An optimised system for rapid auxin-inducible protein degradation in budding yeast . doi: 10.1101/2025.09.24.678194
Schuck, S.; Hubbe, P.; Sharma, C.; Pajonk, O.; Peters, N.; Guschtschin-Schmidt, N.; Friemel, N. . An optimised system for rapid auxin-inducible protein degradation in budding yeast . doi: 10.1101/2025.09.24.678194
Sequences
Provided by depositor
download sequence
ggccggccatttaaatgtttaaacggccacgctggccGAGGAGAACTTCTAGTATATCTACATACCTAATATTATTGCCTTATTAAAAATGGAATCCCAACAATTACATCAAAATCCACATTCTCTTCAAAATCAATTGTCCTGTACTTCCTTGTTCATGTGTGTTCAAAAACGTTATATTTATAGGATAATTATACTCTATTTCTCAACAAGTAATTGGTTGTTTGGCCGAGCGGTCTAAGGCGCCTGATTCAAGAAATATCTTGACCGCAGTTAACTGTGGGAATACTCAGGTATCGTAAGATGCAAGAGTTCGAATCTCTTAGCAACCATTATTTTTTTCCTCAACATAACGAGAACACACAGGGGCGCTATCGCACAGAATCAAATTCGATGACTGGAAATTTTTTGTTAATTTCAGAGGTCGCCTGACGCATATACCTTTTTCAACTGAAAAATTGGGAGAAAAAGGAAAGGTGAGAGCGCCGGAACCGGCTTTTCATATAGAATAGAGAAGCGTTCATGACTAAATGCTTGCATCACAATACTTGAAGTTGACAATATTATTTAAGGACCTATTGTTTTTTCCAATAGGTGGTTAGCAATCGTCTTACTTTCTAACTTTTCTTACCTTTTACATTTCAGCAATATATATATATATATTTCAAGGATATACCATTCTAATGTCTGCCCCTAAGTTACCAATAGATAGCGGTTTAATATAAGGTTTCAACAAGAAATATTTTGTGAAAATCAAAAGTTAATAGTTTCACTGTCGAAAATATGAAAGTTTAAGTTTTCAGTTCATCCCATTGTAGTTATCAAATTTCTGTGTTTCCCGTATATGTGTGCTCTGTATTCATCGCTATCTTGAAACTGGTTATGATCGTTGCGCATGTGACCTGTATCGCTCAAAAGAGCCCGGCACCGGTACCGGGCAAAAGCCCCTAGCAAGCTAAAATGATTCAACTTTCGTAGTAATTTGTTCAACCGGTCACAGTAAAGTCAATGACTAACTTTTGAATCGAGGATGTATTTCCAATTGAAAGCAATGTATATATAAACCATTGTGTTGAAGCTTGTCTTACAGTTTTCTTGTATCCTCCCATTGTTCATATAGTACTTAAAGTACCATAATCCTTAAAGAACAAACTTGAAAGAGAATACACTATACACAACTAATAAATAAAGAATAATCATGGCTGTGACCAAGACAATTGTAGTTCTACCAGGTGACCATGTTGGTCAAGAAATCACTGAAGAGGCCATTAAAGTTTTAAATGCTATTCAGGAATGTCGTCCAGACAAGGTCAATTTCAAGTTTGATCATCATTTGATCGGTGGTGCTGCAATTGATGCCACTGGTGTTCCATTACCAGACGAAGCTTTGGAGGCCTCTAAGAAAGCTGATGCCGTGCTTCTGGGTGCTGTTGGTGGTCCAAAATGGGGTACTGGCGCTGTCAGACCAGAACAAGGTCTTTTGAAAATCCGTAAGGAGTTGCAATTGTATGCTAATCTAAGACCATGTAATTTTGCATCTGATTCCTTACTAGATCTATCGCCATTGAAGCCTGAAATTGCAAGAGGTACAGATTTCGTCGTTGTTAGAGAACTAGTGGGTGGTATTTATTTCGGGGAGAGAAAAGAAGATGAAGGTGATGGTGTCGCCTGGGATAGCGAAAAGTATTCTGTGCCCGAAGTTCAAAGAATCACTAGAATGGCTGCTTTCATGGCTCTGCAACACAACCCACCTTTACCTATTTGGTCTCTAGATAAAGCCAACGTTCTAGCATCATCAAGATTATGGAGAAAAACAGTGGAAGAGACGATTAAGAATGAATTCCCACAATTGACTGTTCAACATCAATTGATTGATTCCGCTGCTATGATCTTGGTTAAGAACCCAACTCATCTAAATGGTATTATTATCACAAACAATATGTTTGGTGATATTATCTCCGATGAAGCCTCCGTGATCCCAGGTTCGTTAGGATTACTTCCATCTGCCTCCTTGGCATCTCTTCCAGATAAGAATACGGCATTTGGTTTATATGAGCCATGTCATGGCTCTGCTCCAGACTTACCAAAGGGAAAGGTTAACCCCGTAGCAACAATCCTATCTGCTGCTATGATGTTGAAGTTGTCTCTGGACCTGTTCGAAGAAGGTGAAATTATAGAACAAGCAGTCAAGAAAGTGTTGGATTCTGGTATCAGAACAGCGGATCTAAAGGGTACCAACTCTACTACTGAGGTCGGTGATGCAGTAGCGAAAGCTGTCAGGGAACTATTAGCTTAGACTTAGTAGATGTACAGTTGCATACATAAGTCTGAACAGAAAGAAGTAAATATCATATCTAGTTCGGTTTAGAAATCAGTGACTATATTGGTTTTGACCAGATCGCAATCCTTGCAAGCATTTAGTAATGACTATCAAACCGGTGAATCATCTGATAAGGCTATATTAGCTTGTGCATTCGCATGTATCGGGAAACGAACGGTACCGggcccATCCTTTTGTTGTTTCCGGGTGTACAATATGGACTTCCTCTTTTCTGGCAACCAAACCCATACATCGGGATTCCTATAATACCTTCGTTGGTCTCCCTAACATGTAGGTGGCGGAGGGGAGATATACAATAGAACAGATACCAGACAAGACATAATGGGCTAAACAAGACTACACCAATTACACTGCCTCATTGATGGTGGTACATAACGAACTAATACTGTAGCCCTAGACTTGATAGCCATCATCATATCGAAGTTTCACTACCCTTTTTCCATTTGCCATCTATTGAAGTAATAATAGGCGCATGCAACTTCTTTTCTTTTTTTTTCTTTTCTCTCTCCCCCGTTGTTGTCTCACCATATCCGCAATGACAAAAAAATGATGGAAGACACTAAAGGAAAAAATTAACGACAAAGACAGCACCAACAGATGTCGTTGTTCCAGAGCTGATGAGGGGTATCTCGAAGCACACGAAACTTTTTCCTTCCTTCATTCACGCACACTACTCTCTAATGAGCAACGGTATACGGCCTTCCTTCCAGTTACTTGAATTTGAAATAAAAAAAAGTTTGCTGTCTTGCTATCAAGTATAAATAGACCTGCAATTATTAATCTTTTGTTTCCTCGTCATTGTTCTCGTTCCCTTTCTTCCTTGTTTCTTTTTCTGCACAATATTTCAAGCTATACCAAGCATACAATCAACTCCAAGCTGGCCGCTctagaActagtATGAACTACTTTCCCGATGAGGTCATTGAGCACGTCTTTGATTTCGTCACAAGCCACAAGGATAGGAACGCCATTAGCCTGGTCTGTAAGAGCTGGTACAAGATCGAGCGGTATTCCAGACAGAAGGTGTTCATCGGCAACTGCTACGCCATCAATCCAGAGAGGCTGCTGCGGAGATTTCCCTGTCTGAAGAGCCTGACCCTGAAGGGCAAGCCCCACgccGCCGACTTTAACCTGGTGCCTCACGAGTGGGGAGGATTCGTGCTGCCATGGATCGAGGCCCTGGCACGGTCCAGAGTGGGCCTGGAGGAGCTGAGGCTGAAGCGCATGGTGGTGACAGACGAGTCTCTGGAGCTGCTGTCTCGGAGCTTCGTGAACTTCAAGAGCCTGGTGCTGGTGTCCTGCGAGGGCTTTACCACAGATGGCCTGGCAAGCATCGCAGCAAACTGTAGGCACCTGCGGGACCTGGACCTGCAGGAGAATGAGATCGACGATCACAGAGGCCAGTGGCTGTCCTGCTTCCCCGATACCTGTACCACACTGGTGACACTGAACTTTGCCTGCCTGGAGGGCGAGACAAATCTGGTGGCCCTGGAGAGGCTGGTGGCACGCTCTCCCAACCTGAAGAGCCTGAAGCTGAATAGGGCAGTGCCTCTGGACGCACTGGCAAGACTGATGGCATGCGCACCACAGATCGTGGATCTGGGCGTGGGCTCCTACGAGAACGACCCTGATTCCGAGTCTTATCTGAAGCTGATGGCCGTGATCAAGAAGTGTACCAGCCTGCGCAGCCTGTCCGGCTTCCTGGAGGCAGCACCTCACTGCCTGTCCGCCTTTCACCCAATCTGTCACAACCTGACATCCCTGAATCTGTCTTACGCCGCCGAGATCCACGGCTCCCACCTGATCAAGCTGATCCAGCACTGCAAGAAGCTGCAGAGGCTGTGGATCCTGGACTCCATCGGCGATAAGGGCCTGGAGGTGGTGGCCTCTACCTGTAAGGAGCTGCAGGAGCTGCGCGTGTTCCCATCTGACCTGCTGGGAGGAGGAAACACCGCAGTGACAGAGGAGGGCCTGGTGGCAATCTCTGCCGGATGCCCCAAGCTGCACAGCATCCTGTATTTTTGTCAGCAGATGACCAACGCCGCCCTGGTGACAGTGGCCAAGAACTGCCCCAATTTCATCAGGTTTCGCCTGTGCATCCTGGAGCCCAATAAGCCTGACCACGTGACATCTCAGCCTCTGGATGAGGGCTTCGGCGCCATCGTGAAGGCCTGCAAGAGCCTGAGGCGCCTGTCTCTGAGCGGCCTGCTGACCGACCAGGTGTTCCTGTACATCGGCATGTATGCCAACCAGCTGGAGATGCTGTCTATCGCCTTTGCCGGCGACACAGATAAGGGCATGCTGTACGTGCTGAATGGCTGTAAGAAGATGAAGAAGCTGGAGATCAGAGACAGCCCTTTTGGCGATACCGCCCTGCTGGCAGACGTGAGCAAGTATGAGACAATGCGGAGCCTGTGGATGAGCTCCTGCGAGGTGACCCTGAGCGGCTGTAAGAGACTGGCCGAGAAGGCCCCATGGCTGAACGTGGAGATCATCAACGAGAATGACAACAATAGGATGGAGGAGAATGGCCACGAGGGCCGCCAGAAGGTGGATAAGCTGTATCTGTATAGGACCGTCGTCGGGACTCGGATGGATGCCCCCCCCTTTGTCTGGATTCTGtgagcggcAAGCTTatcgataccgtcgacctcgagtcatgtaattagttatgtcacgcttacattcacgccctccccccacatccgctctaaccgaaaaggaaggagttagacaacctgaagtctaggtccctatttatttttttatagttatgttagtattaagaacgttatttatatttcaaatttttcttttttttctgtacagacgcgtgtacgcatgtaacattatactgaaaaccttgcttgagaaggttttgggacgctcgaaggctttaatttgctggCCACCGAAGTCGGTGATGCTGTCGCCGAAGAAGTTAAGAAAATCCTTGCTTAAAAAGATTCTCTTTTTTTATGATATTTGTACATAAACTTTATAAATGAAATTCATAATAGAAACGACACGAAATTACAAAATGGAATATGTTCATAGGGTAGACGAAACTATATACGCAATCTACATACATTTATCAAGAAGGAGAAAAAGGAGGATGTAAAGGAATACAGGTAAGCAAATTGATACTAATGGCTCAACGTGATAAGGAAAAAGAATTGCACTTTAACATTAATATTGACAAGGAGGAGGGCACCACACAAAAAGTTAGGTGTAACAGAAAATCATGAAACTATGATTCCTAATTTATATATTGGAGGATTTTCTCTAAAAAAAAAAAAATACAACAAATAAAAAACACTCAATGACCTGACCATTTGATGGAGTTTAAGTCAATACCTTCTTGAACCATTTCCCATAATGGTGAAAGTTCCCTCAAGAATTTTACTCTGTCAGAAACGGCCTTAACGACGTAGGCCGGCCATTTAAATGTTTaaacggccacgctggccCAATTCGCCCTATAGTGAGTCGTATTACAATTCACTGGCCGTCGTTTTACAACGTCGTGACTGGGAAAACCCTGGCGTTACCCAACTTAATCGCCTTGCAGCACATCCCCCtTTCGCCAGCTGGCGTAATAGCGAAGAGGCCCGCACCGATCGCCCTTCCCAACAGTTGCGCAGCCTGAATGGCGAATGGACGCGCCCTGTAGCGGCGCATTAAGCGCGGCGGGTGTGGTGGTTACGCGCAGCGTGACCGCTACACTTGCCAGCGCCCTAGCGCCCGCTCCTTTCGCTTTCTTCCCTTCCTTTCTCGCCACGTTCGCCGGCTTTCCCCGTCAAGCTCTAAATCGGGGGCTCCCTTTAGGGTTCCGATTTAGTGCTTTACGGCACCTCGACCCCAAAAAACTTGATTAGGGTGATGGTTCACGTAGTGGGCCATCGCCCTGATAGACGGTTTTTCGCCCTTTGACGTTGGAGTCCACGTTCTTTAATAGTGGACTCTTGTTCCAAACTGGAACAACACTCAACCCTATCTCGGTCTATTCTTTTGATTTATAAGGGATTTTGCCGATTTCGGCCTATTGGTTAAAAAATGAGCTGATTTAACAAAAATTTAACGCGAATTTTAACAAAATATTAACGcTTACAATTTCCTGATGCGGTATTTTCTCCTTACGCATCTGTGCGGTATTTCACACCGCatAGGGTAATAactgatataattaaattgaagctctaatttgtgagtttagtattaggatctctacccttggcgaaaagtcctctgccaacaatgatgatatctgatccaccacttacaacttcgtcgacggttctgtactgctgacccaatatgcgtatatataccaatctaagtctgtgctccttccttcgttcttccttctgttcggagattaccgaatcaaaaaaatttcaaggaaaccgaaatcaaaaaaaagaataaaaaaaaaatgatgaattgaaaaggtggtATGGTGCACTCTCAGTACAATCTGCTCTGATGCCGCATAGTTAAGCCAGCCCCGACACCCGCCAACACCCGCTGACGCGCCCTGACGGGCTTGTCTGCTCCCGGCATCCGCTTACAGACAAGCTGTGACCGTCTCCGGGAGCTGCATGTGTCAGAGGTTTTCACCGTCATCACCGAAACGCGCGAGACGAAAGGGCCTCGTGATACGCCTATTTTTATAGGTTAATGTCATGATAATAATGGTTTCTTAgacgtcggcgcgccGAGGACGTCAGGTGGCACTTTTCGGGGAAATGTGCGCGGAACCCCTATTTGTTTATTTTTCTAAATACATTCAAATATGTATCCGCTCATGAGACAATAACCCTGATAAATGCTTCAATAATATTGAAAAAGGAAGAGTATGAGTATTCAACATTTCCGTGTCGCCCTTATTCCCTTTTTTGCGGCATTTTGCCTTCCTGTTTTTGCTCACCCAGAAACGCTGGTGAAAGTAAAAGATGCTGAAGATCAGTTGGGTGCACGAGTGGGTTACATCGAACTGGATCTCAACAGCGGTAAGATCCTTGAGAGTTTTCGCCCCGAAGAACGTTTTCCAATGATGAGCACTTTTAAAGTTCTGCTATGTGGCGCGGTATTATCCCGTATTGACGCCGGGCAAGAGCAACTCGGTCGCCGCATACACTATTCTCAGAATGACTTGGTTGAGTACTCACCAGTCACAGAAAAGCATCTTACGGATGGCATGACAGTAAGAGAATTATGCAGTGCTGCCATAACCATGAGTGATAACACTGCGGCCAACTTACTTCTGACAACGATCGGAGGACCGAAGGAGCTAACCGCTTTTTTgCACAACATGGGGGATCATGTAACTCGCCTTGATCGTTGGGAACCGGAGCTGAATGAAGCCATACCAAACGACGAGCGTGACACCACGATGCCTGTAGCAATGGCAACAACGTTGCGCAAACTATTAACTGGCGAACTACTTACTCTAGCTTCCCGGCAACAATTAATAGACTGGATGGAGGCGGATAAAGTTGCAGGACCACTTCTGCGCTCGGCCCTTCCGGCTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGgAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAACTGTCAGACCAAGTTTACTCATATATACTTTAGATTGATTTAAAACTTCATTTTTAATTTAAAAGGATCTAGGTGAAGATCCTTTTTGATAATCTCATGACCAAAATCCCTTAACGTGAGTTTTCGTTCCACTGAGCGTCAGACCCCGTAGAAAAGATCAAAGGATCTTCTTGAGATCCTTTTTTTCTGCGCGTAATCTGCTGCTTGCAAACAAAAAAACCACCGCTACCAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTtCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCGCAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGCCCAGCTTGGAGCGAACGACCTACACCGAACTGAGATACCTACAGCGTGAGCtaTGAGAAAGCGCCACGCTTCCCGAAGGGAGAAAGGCGGACAGGTATCCGGTAAGCGGCAGGGTCGGAACAGGAGAGCGCACGAGGGAGCTTCCAGGGGGaAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCgGAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTCACATGTTCTTTCCTGCGTTATCCCCTGATTCTGTGGATAACCGTATTACCGCCTTTGAGTGAGCTGATACCGCTCGCCGCAGCCGAACGACCGAGCGCAGCGAGTCAGTGAGCGAGGAAGCGGAAGAGCGCCCAATACGCAAACCGCCTCTCCCCGCGCGTTGGCCGATTCATTAATGCAGCTGGCACGACAGGTTTCCCGACTGGAAAGCGGGCAGTGAGCGCAACGCAATTAATGTGAGTTAgCTCACTCATTAGGCACCCCAGGCTTTACACTTTATGCTTCCGGCTCCTATGTTGTGTGGAATTGTGAGCGGATAACAATTTCACACAGGAAACAGCTATGACCATGATTACGCCAAGCTCGGAATTAACCCTCACTAAAGGGAACAAAAGCTG
ggccggccatttaaatgtttaaacggccacgctggccGAGGAGAACTTCTAGTATATCTACATACCTAATATTATTGCCTTATTAAAAATGGAATCCCAACAATTACATCAAAATCCACATTCTCTTCAAAATCAATTGTCCTGTACTTCCTTGTTCATGTGTGTTCAAAAACGTTATATTTATAGGATAATTATACTCTATTTCTCAACAAGTAATTGGTTGTTTGGCCGAGCGGTCTAAGGCGCCTGATTCAAGAAATATCTTGACCGCAGTTAACTGTGGGAATACTCAGGTATCGTAAGATGCAAGAGTTCGAATCTCTTAGCAACCATTATTTTTTTCCTCAACATAACGAGAACACACAGGGGCGCTATCGCACAGAATCAAATTCGATGACTGGAAATTTTTTGTTAATTTCAGAGGTCGCCTGACGCATATACCTTTTTCAACTGAAAAATTGGGAGAAAAAGGAAAGGTGAGAGCGCCGGAACCGGCTTTTCATATAGAATAGAGAAGCGTTCATGACTAAATGCTTGCATCACAATACTTGAAGTTGACAATATTATTTAAGGACCTATTGTTTTTTCCAATAGGTGGTTAGCAATCGTCTTACTTTCTAACTTTTCTTACCTTTTACATTTCAGCAATATATATATATATATTTCAAGGATATACCATTCTAATGTCTGCCCCTAAGTTACCAATAGATAGCGGTTTAATATAAGGTTTCAACAAGAAATATTTTGTGAAAATCAAAAGTTAATAGTTTCACTGTCGAAAATATGAAAGTTTAAGTTTTCAGTTCATCCCATTGTAGTTATCAAATTTCTGTGTTTCCCGTATATGTGTGCTCTGTATTCATCGCTATCTTGAAACTGGTTATGATCGTTGCGCATGTGACCTGTATCGCTCAAAAGAGCCCGGCACCGGTACCGGGCAAAAGCCCCTAGCAAGCTAAAATGATTCAACTTTCGTAGTAATTTGTTCAACCGGTCACAGTAAAGTCAATGACTAACTTTTGAATCGAGGATGTATTTCCAATTGAAAGCAATGTATATATAAACCATTGTGTTGAAGCTTGTCTTACAGTTTTCTTGTATCCTCCCATTGTTCATATAGTACTTAAAGTACCATAATCCTTAAAGAACAAACTTGAAAGAGAATACACTATACACAACTAATAAATAAAGAATAATCATGGCTGTGACCAAGACAATTGTAGTTCTACCAGGTGACCATGTTGGTCAAGAAATCACTGAAGAGGCCATTAAAGTTTTAAATGCTATTCAGGAATGTCGTCCAGACAAGGTCAATTTCAAGTTTGATCATCATTTGATCGGTGGTGCTGCAATTGATGCCACTGGTGTTCCATTACCAGACGAAGCTTTGGAGGCCTCTAAGAAAGCTGATGCCGTGCTTCTGGGTGCTGTTGGTGGTCCAAAATGGGGTACTGGCGCTGTCAGACCAGAACAAGGTCTTTTGAAAATCCGTAAGGAGTTGCAATTGTATGCTAATCTAAGACCATGTAATTTTGCATCTGATTCCTTACTAGATCTATCGCCATTGAAGCCTGAAATTGCAAGAGGTACAGATTTCGTCGTTGTTAGAGAACTAGTGGGTGGTATTTATTTCGGGGAGAGAAAAGAAGATGAAGGTGATGGTGTCGCCTGGGATAGCGAAAAGTATTCTGTGCCCGAAGTTCAAAGAATCACTAGAATGGCTGCTTTCATGGCTCTGCAACACAACCCACCTTTACCTATTTGGTCTCTAGATAAAGCCAACGTTCTAGCATCATCAAGATTATGGAGAAAAACAGTGGAAGAGACGATTAAGAATGAATTCCCACAATTGACTGTTCAACATCAATTGATTGATTCCGCTGCTATGATCTTGGTTAAGAACCCAACTCATCTAAATGGTATTATTATCACAAACAATATGTTTGGTGATATTATCTCCGATGAAGCCTCCGTGATCCCAGGTTCGTTAGGATTACTTCCATCTGCCTCCTTGGCATCTCTTCCAGATAAGAATACGGCATTTGGTTTATATGAGCCATGTCATGGCTCTGCTCCAGACTTACCAAAGGGAAAGGTTAACCCCGTAGCAACAATCCTATCTGCTGCTATGATGTTGAAGTTGTCTCTGGACCTGTTCGAAGAAGGTGAAATTATAGAACAAGCAGTCAAGAAAGTGTTGGATTCTGGTATCAGAACAGCGGATCTAAAGGGTACCAACTCTACTACTGAGGTCGGTGATGCAGTAGCGAAAGCTGTCAGGGAACTATTAGCTTAGACTTAGTAGATGTACAGTTGCATACATAAGTCTGAACAGAAAGAAGTAAATATCATATCTAGTTCGGTTTAGAAATCAGTGACTATATTGGTTTTGACCAGATCGCAATCCTTGCAAGCATTTAGTAATGACTATCAAACCGGTGAATCATCTGATAAGGCTATATTAGCTTGTGCATTCGCATGTATCGGGAAACGAACGGTACCGggcccATCCTTTTGTTGTTTCCGGGTGTACAATATGGACTTCCTCTTTTCTGGCAACCAAACCCATACATCGGGATTCCTATAATACCTTCGTTGGTCTCCCTAACATGTAGGTGGCGGAGGGGAGATATACAATAGAACAGATACCAGACAAGACATAATGGGCTAAACAAGACTACACCAATTACACTGCCTCATTGATGGTGGTACATAACGAACTAATACTGTAGCCCTAGACTTGATAGCCATCATCATATCGAAGTTTCACTACCCTTTTTCCATTTGCCATCTATTGAAGTAATAATAGGCGCATGCAACTTCTTTTCTTTTTTTTTCTTTTCTCTCTCCCCCGTTGTTGTCTCACCATATCCGCAATGACAAAAAAATGATGGAAGACACTAAAGGAAAAAATTAACGACAAAGACAGCACCAACAGATGTCGTTGTTCCAGAGCTGATGAGGGGTATCTCGAAGCACACGAAACTTTTTCCTTCCTTCATTCACGCACACTACTCTCTAATGAGCAACGGTATACGGCCTTCCTTCCAGTTACTTGAATTTGAAATAAAAAAAAGTTTGCTGTCTTGCTATCAAGTATAAATAGACCTGCAATTATTAATCTTTTGTTTCCTCGTCATTGTTCTCGTTCCCTTTCTTCCTTGTTTCTTTTTCTGCACAATATTTCAAGCTATACCAAGCATACAATCAACTCCAAGCTGGCCGCTctagaActagtATGAACTACTTTCCCGATGAGGTCATTGAGCACGTCTTTGATTTCGTCACAAGCCACAAGGATAGGAACGCCATTAGCCTGGTCTGTAAGAGCTGGTACAAGATCGAGCGGTATTCCAGACAGAAGGTGTTCATCGGCAACTGCTACGCCATCAATCCAGAGAGGCTGCTGCGGAGATTTCCCTGTCTGAAGAGCCTGACCCTGAAGGGCAAGCCCCACgccGCCGACTTTAACCTGGTGCCTCACGAGTGGGGAGGATTCGTGCTGCCATGGATCGAGGCCCTGGCACGGTCCAGAGTGGGCCTGGAGGAGCTGAGGCTGAAGCGCATGGTGGTGACAGACGAGTCTCTGGAGCTGCTGTCTCGGAGCTTCGTGAACTTCAAGAGCCTGGTGCTGGTGTCCTGCGAGGGCTTTACCACAGATGGCCTGGCAAGCATCGCAGCAAACTGTAGGCACCTGCGGGACCTGGACCTGCAGGAGAATGAGATCGACGATCACAGAGGCCAGTGGCTGTCCTGCTTCCCCGATACCTGTACCACACTGGTGACACTGAACTTTGCCTGCCTGGAGGGCGAGACAAATCTGGTGGCCCTGGAGAGGCTGGTGGCACGCTCTCCCAACCTGAAGAGCCTGAAGCTGAATAGGGCAGTGCCTCTGGACGCACTGGCAAGACTGATGGCATGCGCACCACAGATCGTGGATCTGGGCGTGGGCTCCTACGAGAACGACCCTGATTCCGAGTCTTATCTGAAGCTGATGGCCGTGATCAAGAAGTGTACCAGCCTGCGCAGCCTGTCCGGCTTCCTGGAGGCAGCACCTCACTGCCTGTCCGCCTTTCACCCAATCTGTCACAACCTGACATCCCTGAATCTGTCTTACGCCGCCGAGATCCACGGCTCCCACCTGATCAAGCTGATCCAGCACTGCAAGAAGCTGCAGAGGCTGTGGATCCTGGACTCCATCGGCGATAAGGGCCTGGAGGTGGTGGCCTCTACCTGTAAGGAGCTGCAGGAGCTGCGCGTGTTCCCATCTGACCTGCTGGGAGGAGGAAACACCGCAGTGACAGAGGAGGGCCTGGTGGCAATCTCTGCCGGATGCCCCAAGCTGCACAGCATCCTGTATTTTTGTCAGCAGATGACCAACGCCGCCCTGGTGACAGTGGCCAAGAACTGCCCCAATTTCATCAGGTTTCGCCTGTGCATCCTGGAGCCCAATAAGCCTGACCACGTGACATCTCAGCCTCTGGATGAGGGCTTCGGCGCCATCGTGAAGGCCTGCAAGAGCCTGAGGCGCCTGTCTCTGAGCGGCCTGCTGACCGACCAGGTGTTCCTGTACATCGGCATGTATGCCAACCAGCTGGAGATGCTGTCTATCGCCTTTGCCGGCGACACAGATAAGGGCATGCTGTACGTGCTGAATGGCTGTAAGAAGATGAAGAAGCTGGAGATCAGAGACAGCCCTTTTGGCGATACCGCCCTGCTGGCAGACGTGAGCAAGTATGAGACAATGCGGAGCCTGTGGATGAGCTCCTGCGAGGTGACCCTGAGCGGCTGTAAGAGACTGGCCGAGAAGGCCCCATGGCTGAACGTGGAGATCATCAACGAGAATGACAACAATAGGATGGAGGAGAATGGCCACGAGGGCCGCCAGAAGGTGGATAAGCTGTATCTGTATAGGACCGTCGTCGGGACTCGGATGGATGCCCCCCCCTTTGTCTGGATTCTGtgagcggcAAGCTTatcgataccgtcgacctcgagtcatgtaattagttatgtcacgcttacattcacgccctccccccacatccgctctaaccgaaaaggaaggagttagacaacctgaagtctaggtccctatttatttttttatagttatgttagtattaagaacgttatttatatttcaaatttttcttttttttctgtacagacgcgtgtacgcatgtaacattatactgaaaaccttgcttgagaaggttttgggacgctcgaaggctttaatttgctggCCACCGAAGTCGGTGATGCTGTCGCCGAAGAAGTTAAGAAAATCCTTGCTTAAAAAGATTCTCTTTTTTTATGATATTTGTACATAAACTTTATAAATGAAATTCATAATAGAAACGACACGAAATTACAAAATGGAATATGTTCATAGGGTAGACGAAACTATATACGCAATCTACATACATTTATCAAGAAGGAGAAAAAGGAGGATGTAAAGGAATACAGGTAAGCAAATTGATACTAATGGCTCAACGTGATAAGGAAAAAGAATTGCACTTTAACATTAATATTGACAAGGAGGAGGGCACCACACAAAAAGTTAGGTGTAACAGAAAATCATGAAACTATGATTCCTAATTTATATATTGGAGGATTTTCTCTAAAAAAAAAAAAATACAACAAATAAAAAACACTCAATGACCTGACCATTTGATGGAGTTTAAGTCAATACCTTCTTGAACCATTTCCCATAATGGTGAAAGTTCCCTCAAGAATTTTACTCTGTCAGAAACGGCCTTAACGACGTAGGCCGGCCATTTAAATGTTTaaacggccacgctggccCAATTCGCCCTATAGTGAGTCGTATTACAATTCACTGGCCGTCGTTTTACAACGTCGTGACTGGGAAAACCCTGGCGTTACCCAACTTAATCGCCTTGCAGCACATCCCCCtTTCGCCAGCTGGCGTAATAGCGAAGAGGCCCGCACCGATCGCCCTTCCCAACAGTTGCGCAGCCTGAATGGCGAATGGACGCGCCCTGTAGCGGCGCATTAAGCGCGGCGGGTGTGGTGGTTACGCGCAGCGTGACCGCTACACTTGCCAGCGCCCTAGCGCCCGCTCCTTTCGCTTTCTTCCCTTCCTTTCTCGCCACGTTCGCCGGCTTTCCCCGTCAAGCTCTAAATCGGGGGCTCCCTTTAGGGTTCCGATTTAGTGCTTTACGGCACCTCGACCCCAAAAAACTTGATTAGGGTGATGGTTCACGTAGTGGGCCATCGCCCTGATAGACGGTTTTTCGCCCTTTGACGTTGGAGTCCACGTTCTTTAATAGTGGACTCTTGTTCCAAACTGGAACAACACTCAACCCTATCTCGGTCTATTCTTTTGATTTATAAGGGATTTTGCCGATTTCGGCCTATTGGTTAAAAAATGAGCTGATTTAACAAAAATTTAACGCGAATTTTAACAAAATATTAACGcTTACAATTTCCTGATGCGGTATTTTCTCCTTACGCATCTGTGCGGTATTTCACACCGCatAGGGTAATAactgatataattaaattgaagctctaatttgtgagtttagtattaggatctctacccttggcgaaaagtcctctgccaacaatgatgatatctgatccaccacttacaacttcgtcgacggttctgtactgctgacccaatatgcgtatatataccaatctaagtctgtgctccttccttcgttcttccttctgttcggagattaccgaatcaaaaaaatttcaaggaaaccgaaatcaaaaaaaagaataaaaaaaaaatgatgaattgaaaaggtggtATGGTGCACTCTCAGTACAATCTGCTCTGATGCCGCATAGTTAAGCCAGCCCCGACACCCGCCAACACCCGCTGACGCGCCCTGACGGGCTTGTCTGCTCCCGGCATCCGCTTACAGACAAGCTGTGACCGTCTCCGGGAGCTGCATGTGTCAGAGGTTTTCACCGTCATCACCGAAACGCGCGAGACGAAAGGGCCTCGTGATACGCCTATTTTTATAGGTTAATGTCATGATAATAATGGTTTCTTAgacgtcggcgcgccGAGGACGTCAGGTGGCACTTTTCGGGGAAATGTGCGCGGAACCCCTATTTGTTTATTTTTCTAAATACATTCAAATATGTATCCGCTCATGAGACAATAACCCTGATAAATGCTTCAATAATATTGAAAAAGGAAGAGTATGAGTATTCAACATTTCCGTGTCGCCCTTATTCCCTTTTTTGCGGCATTTTGCCTTCCTGTTTTTGCTCACCCAGAAACGCTGGTGAAAGTAAAAGATGCTGAAGATCAGTTGGGTGCACGAGTGGGTTACATCGAACTGGATCTCAACAGCGGTAAGATCCTTGAGAGTTTTCGCCCCGAAGAACGTTTTCCAATGATGAGCACTTTTAAAGTTCTGCTATGTGGCGCGGTATTATCCCGTATTGACGCCGGGCAAGAGCAACTCGGTCGCCGCATACACTATTCTCAGAATGACTTGGTTGAGTACTCACCAGTCACAGAAAAGCATCTTACGGATGGCATGACAGTAAGAGAATTATGCAGTGCTGCCATAACCATGAGTGATAACACTGCGGCCAACTTACTTCTGACAACGATCGGAGGACCGAAGGAGCTAACCGCTTTTTTgCACAACATGGGGGATCATGTAACTCGCCTTGATCGTTGGGAACCGGAGCTGAATGAAGCCATACCAAACGACGAGCGTGACACCACGATGCCTGTAGCAATGGCAACAACGTTGCGCAAACTATTAACTGGCGAACTACTTACTCTAGCTTCCCGGCAACAATTAATAGACTGGATGGAGGCGGATAAAGTTGCAGGACCACTTCTGCGCTCGGCCCTTCCGGCTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGgAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAACTGTCAGACCAAGTTTACTCATATATACTTTAGATTGATTTAAAACTTCATTTTTAATTTAAAAGGATCTAGGTGAAGATCCTTTTTGATAATCTCATGACCAAAATCCCTTAACGTGAGTTTTCGTTCCACTGAGCGTCAGACCCCGTAGAAAAGATCAAAGGATCTTCTTGAGATCCTTTTTTTCTGCGCGTAATCTGCTGCTTGCAAACAAAAAAACCACCGCTACCAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTtCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCGCAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGCCCAGCTTGGAGCGAACGACCTACACCGAACTGAGATACCTACAGCGTGAGCtaTGAGAAAGCGCCACGCTTCCCGAAGGGAGAAAGGCGGACAGGTATCCGGTAAGCGGCAGGGTCGGAACAGGAGAGCGCACGAGGGAGCTTCCAGGGGGaAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCgGAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTCACATGTTCTTTCCTGCGTTATCCCCTGATTCTGTGGATAACCGTATTACCGCCTTTGAGTGAGCTGATACCGCTCGCCGCAGCCGAACGACCGAGCGCAGCGAGTCAGTGAGCGAGGAAGCGGAAGAGCGCCCAATACGCAAACCGCCTCTCCCCGCGCGTTGGCCGATTCATTAATGCAGCTGGCACGACAGGTTTCCCGACTGGAAAGCGGGCAGTGAGCGCAACGCAATTAATGTGAGTTAgCTCACTCATTAGGCACCCCAGGCTTTACACTTTATGCTTCCGGCTCCTATGTTGTGTGGAATTGTGAGCGGATAACAATTTCACACAGGAAACAGCTATGACCATGATTACGCCAAGCTCGGAATTAACCCTCACTAAAGGGAACAAAAGCTG
