EPR Plasmid Kit #2: makeTCR murine TR*V modules
The makeTCR system enables 100% fidelity assembly TCR assembly into a range of pre-made TCR expression vectors including lentivirus, AAV, sleeping beauty, HDR cassettes, and the Gateway[TM] system.
TRAV, TRBV, TRGV and TRDV modules are available, as are a range of endogenous and engineered TCR constant domains. All modules have been domesticated to remove BsmBI, BsaI and SwaI sites. Modules can be further amplified by rolling circle amplification in plate format to facilitate propagation.
| Purpose | Murine TRAV and TRBV genes encoded as modules flanked with BsmBI sites for modular TCR cloning using the makeTCR system |
| Depositing Lab |
Michael Platten Deutsches Krebsforschungszentrum (DKFZ) |
| Depositing Scientist | Edward Green |
| Reference | This plasmid kit is from an unpublished project. Please acknowledge the depositing scientist. |
Detailed description
Murine TCRs can be rapidly and at low cost using pre-cloned TR*V and TR*C modules. The makeTCR system provides a software platform (https://makeTCR.com) to facilitate the process of cloning TCR expression vectors from TCR repertoire files.The makeTCR system enables 100% fidelity assembly TCR assembly into a range of pre-made TCR expression vectors including lentivirus, AAV, sleeping beauty, HDR cassettes, and the Gateway[TM] system.
TRAV, TRBV, TRGV and TRDV modules are available, as are a range of endogenous and engineered TCR constant domains. All modules have been domesticated to remove BsmBI, BsaI and SwaI sites. Modules can be further amplified by rolling circle amplification in plate format to facilitate propagation.
Documents
| Document | Description |
|---|---|
| Excel sheet with mapping of plasmids to plate wells |
EPR Plasmids_positions.xlsx
|
How to cite
In the Materials & Methods:
The plasmid kit "makeTCR murine TR*V modules" was a gift from Michael Platten (European Plasmid Repository kit #2).
In the References:
No reference available. (This plasmid is not yet published.)
The plasmid kit "makeTCR murine TR*V modules" was a gift from Michael Platten (European Plasmid Repository kit #2).
In the References:
No reference available. (This plasmid is not yet published.)
Plasmids
| Plasmid # | Plasmid name | MTA and Terms |
|---|---|---|
| 756 | Trbv31 |
MTA (PDF download) |
| 757 | Trav4-4-dv10 |
MTA (PDF download) |
| 758 | Trbv13-2 |
MTA (PDF download) |
| 759 | Trav16n |
MTA (PDF download) |
| 760 | Trbv19 |
MTA (PDF download) |
| 761 | Trav1 |
MTA (PDF download) |
| 762 | Trbv3 |
MTA (PDF download) |
| 763 | Trav7-3 |
MTA (PDF download) |
| 764 | Trav7d-6 |
MTA (PDF download) |
| 765 | Trav12-3 |
MTA (PDF download) |
| 766 | Trav14-3 |
MTA (PDF download) |
| 767 | Trav13d-2 |
MTA (PDF download) |
| 768 | Trbv12-1 |
MTA (PDF download) |
| 769 | Trav7d-2 |
MTA (PDF download) |
| 770 | Trbv16 |
MTA (PDF download) |
| 771 | Trav17 |
MTA (PDF download) |
| 772 | Trav6-6 |
MTA (PDF download) |
| 773 | Trav7-4 |
MTA (PDF download) |
| 774 | Trbv13-1 |
MTA (PDF download) |
| 775 | Trbv14 |
MTA (PDF download) |
| 776 | Trav16d-dv11 |
MTA (PDF download) |
| 777 | Trav6-2 |
MTA (PDF download) |
| 778 | Trav8d-1 |
MTA (PDF download) |
| 779 | Trbv13-3 |
MTA (PDF download) |
| 780 | Trav9n-4 |
MTA (PDF download) |
| 781 | Trav13-4-dv7 |
MTA (PDF download) |
| 782 | Trav6-1 |
MTA (PDF download) |
| 783 | Trav13n-1 |
MTA (PDF download) |
| 784 | Trav13-2 |
MTA (PDF download) |
| 785 | Trbv2 |
MTA (PDF download) |
| 786 | Trav14d-2 |
MTA (PDF download) |
| 787 | Trbv20 |
MTA (PDF download) |
| 788 | Trav14n-3 |
MTA (PDF download) |
| 789 | Trav7n-6 |
MTA (PDF download) |
| 790 | Trav3-4 |
MTA (PDF download) |
| 791 | Trav6d-7 |
MTA (PDF download) |
| 792 | Trav13-1 |
MTA (PDF download) |
| 793 | Trbv5 |
MTA (PDF download) |
| 794 | Trav16 |
MTA (PDF download) |
| 795 | Trav10 |
MTA (PDF download) |
| 796 | Trav6n-6 |
MTA (PDF download) |
| 797 | Trav13d-1 |
MTA (PDF download) |
| 798 | Trav4d-3 |
MTA (PDF download) |
| 799 | Trbv29 |
MTA (PDF download) |
| 800 | Trav12-1 |
MTA (PDF download) |
| 801 | Trav12d-2 |
MTA (PDF download) |
| 802 | Trav8n-2 |
MTA (PDF download) |
| 803 | Trbv30 |
MTA (PDF download) |
| 804 | Trbv4 |
MTA (PDF download) |
| 805 | Trav13d-4 |
MTA (PDF download) |
| 806 | Trav2 |
MTA (PDF download) |
| 807 | Trav10n |
MTA (PDF download) |
| 808 | Trav7-6 |
MTA (PDF download) |
| 809 | Trav6n-7 |
MTA (PDF download) |
| 810 | Trbv26 |
MTA (PDF download) |
| 811 | Trbv17 |
MTA (PDF download) |
| 812 | Trav7-1 |
MTA (PDF download) |
| 813 | Trav3-3 |
MTA (PDF download) |
| 814 | Trav5-1 |
MTA (PDF download) |
| 815 | Trav10d |
MTA (PDF download) |
| 816 | Trav6-5 |
MTA (PDF download) |
| 817 | Trav8d-2 |
MTA (PDF download) |
| 818 | Trav12d-3 |
MTA (PDF download) |
| 819 | Trav12d-1 |
MTA (PDF download) |
| 820 | Trbv23 |
MTA (PDF download) |
| 821 | Trav7d-5 |
MTA (PDF download) |
| 822 | Trav6-4 |
MTA (PDF download) |
| 823 | Trav12-2 |
MTA (PDF download) |
| 824 | Trav8-1 |
MTA (PDF download) |
| 825 | Trav6-3 |
MTA (PDF download) |
| 826 | Trav3-1 |
MTA (PDF download) |
| 827 | Trav4d-4 |
MTA (PDF download) |
| 828 | Trav19 |
MTA (PDF download) |
| 829 | Trav14-2 |
MTA (PDF download) |
| 830 | Trav4-3 |
MTA (PDF download) |
| 831 | Trav14-1 |
MTA (PDF download) |
| 832 | Trav14d-1 |
MTA (PDF download) |
| 833 | Trav13n-4 |
MTA (PDF download) |
| 834 | Trav6-7-dv9 |
MTA (PDF download) |
| 835 | Trav14d-3-dv8 |
MTA (PDF download) |
| 836 | Trav15-1-dv6-1 |
MTA (PDF download) |
| 837 | Trav15-2-dv6-2 |
MTA (PDF download) |
| 838 | Trav5-4 |
MTA (PDF download) |
| 839 | Trav5d-4 |
MTA (PDF download) |
| 840 | Trav3d-3 |
MTA (PDF download) |
| 841 | Trbv1 |
MTA (PDF download) |
| 842 | Trbv15 |
MTA (PDF download) |
| 843 | Trav9-1 |
MTA (PDF download) |
| 844 | Trav7-5 |
MTA (PDF download) |
| 845 | Trbv12-2 |
MTA (PDF download) |
| 846 | Trav6d-4 |
MTA (PDF download) |
| 847 | Trav9-2 |
MTA (PDF download) |
| 848 | Trav9d-3 |
MTA (PDF download) |
| 849 | Trav7-2 |
MTA (PDF download) |