| Purpose | Catch & Release vectors streamline cloning and transgenic plant selection through the Fluorescence-Accumulating Seed Technology (FAST), which marks positive transformants with a strongly fluorescing seed coat. Each plasmid consists of four, easy to exchange, modules: a plant promoter, a cloning dropout marker, protease cleavage sites, and seven different epitope tags, including an innovative dual-fluorescent tag, providing flexibility for diverse experimental needs. |
| Depositing Lab |
Hans-Henning Kunz Ludwig-Maximilians-Universität München (LMU) |
| Depositing Scientist | Benjamin Brandt |
| Reference |
Catch & Release - rapid cost-effective protein purification from plants using a DIY GFP-Trap-protease approach Schwartz, S.; Engstler, C.; Muehlbauer, S.; Windenbach, E.; Wunder, T.; Kunz, H.-H.; Brandt, B. DOI |
Detailed description
Detailed description of the contents and a cloning guide can be found in the publicationDocuments
| Document | Description |
|---|---|
| Excel sheet with mapping of plasmids to plate wells |
Plate layout.xlsx
|
How to cite
In the Materials & Methods:
The plasmid kit "Catch & Release vector set" was a gift from Hans-Henning Kunz (European Plasmid Repository kit #3).
In the References:
Schwartz, S.; Engstler, C.; Muehlbauer, S.; Windenbach, E.; Wunder, T.; Kunz, H.-H.; Brandt, B. . Catch & Release - rapid cost-effective protein purification from plants using a DIY GFP-Trap-protease approach . doi: 10.1101/2025.05.15.654187
The plasmid kit "Catch & Release vector set" was a gift from Hans-Henning Kunz (European Plasmid Repository kit #3).
In the References:
Schwartz, S.; Engstler, C.; Muehlbauer, S.; Windenbach, E.; Wunder, T.; Kunz, H.-H.; Brandt, B. . Catch & Release - rapid cost-effective protein purification from plants using a DIY GFP-Trap-protease approach . doi: 10.1101/2025.05.15.654187