EPR Plasmid #183: pMT-Flag-CG34449
Sequence: download sequence
| Purpose of plasmid | Copper-inducible expression of N-terminally FLAG-tagged Drosophila CG34449 (ZDHHC8) |
| Depositing Lab |
Aurelio Teleman Deutsches Krebsforschungszentrum (DKFZ) |
| Depositing Scientist | Aurelio Teleman |
| Antibiotic Resistance | Ampicillin |
| Bacterial Growth Temp | 37 |
| Copy Number | High |
| Selectable Marker | |
| Retransformation Info: | Retransforms well in XL1blue. |
| Reference |
Drosophila ZDHHC8 palmitoylates scribble and Ras64B and controls growth and viability. Strassburger K et al. PLoS One 2019 PubMed DOI |
| MTA and Terms |
MTA (PDF download) |
How to cite this reagent:
In the Materials & Methods:
pMT-Flag-CG34449 was a gift from Aurelio Teleman (European Plasmid Repository plasmid #183).
In the References:
Strassburger K et al.. Drosophila ZDHHC8 palmitoylates scribble and Ras64B and controls growth and viability.. PLoS One 2019 doi:10.1371/journal.pone.0198149 PubMed 30735487
pMT-Flag-CG34449 was a gift from Aurelio Teleman (European Plasmid Repository plasmid #183).
In the References:
Strassburger K et al.. Drosophila ZDHHC8 palmitoylates scribble and Ras64B and controls growth and viability.. PLoS One 2019 doi:10.1371/journal.pone.0198149 PubMed 30735487
Sequence: download sequence
ggcactggccgtcgttttacaacgtcgtgactgggaaaaccctggcgttacccaacttaatcgccttgcagcacatccccctttcgccagctggcgtaatagcgaagaggcccgcaccgatcgcccttcccaacagttgcgcagcctgaatggcgaatggcgcctgatgcggtattttctccttacgcatctgtgcggtatttcacaccgcatatggtgcactctcagtacaatctgctctgatgccgcatagttaagccagccccgacacccgccaacacccgctgacgcgccctgacgggcttgtctgctcccggcatccgcttacagacaagctgtgaccgtctccgggagctgcatgtgtcagaggttttcaccgtcatcaccgaaacgcgcgagacgaaagggcctcgtgatacgcctatttttataggttaatgtcatgataataatggtttcttagacgtcaggtggcacttttcggggaaatgtgcgcggaacccctatttgtttatttttctaaatacattcaaatatgtatccgctcatgagacaataaccctgataaatgcttcaataatattgaaaaaggaagagtatgagtattcaacatttccgtgtcgcccttattcccttttttgcggcattttgccttcctgtttttgctcacccagaaacgctggtgaaagtaaaagatgctgaagatcagttgggtgcacgagtgggttacatcgaactggatctcaacagcggtaagatccttgagagttttcgccccgaagaacgttttccaatgatgagcacttttaaagttctgctatgtggcgcggtattatcccgtattgacgccgggcaagagcaactcggtcgccgcatacactattctcagaatgacttggttgagtactcaccagtcacagaaaagcatcttacggatggcatgacagtaagagaattatgcagtgctgccataaccatgagtgataacactgcggccaacttacttctgacaacgatcggaggaccgaaggagctaaccgcttttttgcacaacatgggggatcatgtaactcgccttgatcgttgggaaccggagctgaatgaagccataccaaacgacgagcgtgacaccacgatgcctgtagcaatggcaacaacgttgcgcaaactattaactggcgaactacttactctagcttcccggcaacaattaatagactggatggaggcggataaagttgcaggaccacttctgcgctcggcccttccggctggctggtttattgctgataaatctggagccggtgagcgtgggtctcgcggtatcattgcagcactggggccagatggtaagccctcccgtatcgtagttatctacacgacggggagtcaggcaactatggatgaacgaaatagacagatcgctgagataggtgcctcactgattaagcattggtaactgtcagaccaagtttactcatatatactttagattgatttaaaacttcatttttaatttaaaaggatctaggtgaagatcctttttgataatctcatgaccaaaatcccttaacgtgagttttcgttccactgagcgtcagaccccgtagaaaagatcaaaggatcttcttgagatcctttttttctgcgcgtaatctgctgcttgcaaacaaaaaaaccaccgctaccagcggtggtttgtttgccggatcaagagctaccaactctttttccgaaggtaactggcttcagcagagcgcagataccaaatactgtccttctagtgtagccgtagttaggccaccacttcaagaactctgtagcaccgcctacatacctcgctctgctaatcctgttaccagtggctgctgccagtggcgataagtcgtgtcttaccgggttggactcaagacgatagttaccggataaggcgcagcggtcgggctgaacggggggttcgtgcacacagcccagcttggagcgaacgacctacaccgaactgagatacctacagcgtgagctatgagaaagcgccacgcttcccgaagggagaaaggcggacaggtatccggtaagcggcagggtcggaacaggagagcgcacgagggagcttccagggggaaacgcctggtatctttatagtcctgtcgggtttcgccacctctgacttgagcgtcgatttttgtgatgctcgtcaggggggcggagcctatggaaaaacgccagcaacgcggcctttttacggttcctggccttttgctggccttttgctcacatgttctttcctgcgttatcccctgattctgtggataaccgtattaccgcctttgagtgagctgataccgctcgccgcagccgaacgaccgagcgcagcgagtcagtgagcgaggaagcggaagagcgcccaatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggctcgtatgttgtgtggaattgtgagcggataacaatttcacacaggaaacagctatgacatgattaccgttgcaggacaggatgtggtgcccgatgtgactagctctttgctgcaggccgtcctatcctctggttccgataagagacccagaactccggccccccaccgcccaccgccacccccatacatatgtggtacgcaagtaagagtgcctgcgcatgccccatgtgccccaccaagagttttgcatcccatacaagtccccaaagtggagaaccgaaccaattcttcgcgggcagaacaaaagcttctgcacacgtctccactcgaatttggagccggccggcgtgtgcaaaagaggtgaatcgaacgaaagacccgtgtgtaaagccgcgtttccaaaatgtataaaaccgagagcatctggccaatgtgcatcagttgtggtcagcagcaaaatcaagtgaatcatctcagtgcaactaaagggaattATGGACTACAAGGACGACGACGACAAGGAATTCATGCCCAAATGCGATGTGAAAACGCGCTACATTCCTGCGACTTTCGCCTGGATTGTGCTGCTTCTGACCACATTTCTCTTCTTCTTCTATCCCTGCCAGTTCTATGTGAAGAGTCATCCATGGGTGCTGGCCTACCAGGGCGTGATCACATTCTTCGTGCTGGCCAACTTTACGCTGGCCACGTTTATGGATCCGGGCATCATCCCCAAAGCCTCGCCCGACGAAGACTGCGAGGAGGAGCTGCGCGCCCCGCTCTACAAGAATGCCGAGATCAACGGCATCACCGTCAAGATGAAGTGGTGCGTCACCTGTAAGTTCTATCGCCCGCCACGCTGTTCCCACTGCTCCGTTTGCAATCACTGCATAGAGACTTTCGATCATCACTGTCCGTGGGTGAACAACTGCATTGGCAGGCGGAATTACAGATTCTTTTTCTTCTTCCTCGTCTCACTGTCCATTCACATGCTGAGCATCTTCTCGCTGTGCCTGGTCTACGTGCTGAAGATTATGCCCAACATCAAGGACACGGCGCCCATCGTAGCCATTATCCTGATGGGCCTGGTGACCATTTTGGCCATCCCCATCTTCGGACTGACCGGCTTCCACATGGTACTGGTGTCGCGGGGTCGGACAACCAATGAGCAGGTGACGGGAAAATTCAAGGGCGGCTACAATCCGTTCTCGCGCGGCTGTTGGCACAACTGCTGCTACACACAGTTCGGACCTCAGTATCCCAGCCTCCTTAATCCGAAGAAGTACGCATCGCGTCGATCTCAGGTGCAAAACCAGGCGATCAGCACCATTTGCAATGACCGGAGCGGCCAGCAGACAGGCGCCGGGAGCGGTGCCGGCGGCAATGGTACCGCCGCTGTATCCGGTGGCGGCGGTGGAGTGGGCAGCGGTGGCGGTATGCGCGGCACTGCGGTGCAGTACTCACCACGCTCCTTCTACGACGCGAGTCGCGAGAAGCGGGGAATTCAGGTAAAGACATATATGGCCGAGGGCAATGGTTATAATCAACGGTCGGGCAGCACAACGCTTTACAGTAAGCTATCGCCCGGACGAGAGTGCTCCGATACAGACCTGGAGCCGCCGCCCGCATCCCAGAGTCAGGACTGTGAACCGACGCCACCGCTGCAGCGGCACAACTCCAGCAGCTTCTATCTGCCGCAGGTGAGCGATTCTGGCGGCCTTAACGGCAGTGTTTCCACGGGCGGCGGAGGAGGCGGCGACTCGCCGCGCCACATGCGTCTCTACCATCCGCGTCACAGTCCGCATGCGAGGCCCAGGGGTCTTGATCCGCAGCGGGGCTACACGAGCGACGCCCTATCGCCGGACCATCCCGTTGGCTATGGCGTTGGCGTCAATGGATCGCAGCAGCAACAGCAGCAGGCGCTGGCGGCGGCAGCAGCGGCGGCGGCGGTGGCAGCACAAAATCAACGCAGCGCGACGACCACGGCCACGCCCACCATGCAGCAGCGGATTAAGCCGCTGGGCGTGGCCACGCCCCTCGTGATGGCGAGTCCAGTGCGAAGATCTAACCCGGGCACGCCCACTCAACCAAGACGACCCGATTTCATCGGACTGAACGCACAGGCGGTGGCGCAGCAGCAGCAACAGCAACAGCAGGCTGCGGCGGCTGCAGCAGCTGCCTATTACGAATACACCAGTGGTCTGCCGCCGCAACATCCGCAGGCGCCCAGCATTCAACAGCAGCAGCAACAGCTGCTTCTCCAGCAGCAGCGTGTCCTCATGCAGCACCAGCAACAGCAGCAAGCTCTGCAGCAGCAGCAGCAGCAGCAGCAACAGGCGGCGGTACATGCGGCACATCCGCAACATGCCGCTTTAGCGCAGGCGGCGTACGGTGGCAGCCCACAGAGACGCTTCCTCTCCGAGGGCGAACTGGTGCGCCAGGGTGCTGGAGGAGGCGTTGCCGGCGGAGAGTTGTCCTACGCACGATCCAACAATACGGTGGATAATATACGAGAGCTCGCCGGCAGTCCGCAGCGCGGCGTTTACATGTGGAAGGATACGTCGCCGGGATTCACCAACAACGCCGGACAGCAGCAGCAGCAACAGCAGCAGGCACAACAGGTGGTCAGCAGCGGGATTGGCAGCAGTGCGGGCACGCTGTCCAGCAGCGGAGCCGCCGCCGGAGTGATGCCGCATGCCCAGTACATGACGGCGGGCGGAGGAGCACATCCGCTGATTATGACGCACTCGCGACTCCAGGACTACACCATCCAGCAGCAGCAGCAGCAGCAGCAACAACAGGCGGCGGCTGCGGCAGCAGCCTCATATCATCGCTCCAATCCCACGAGTCCCACCACCATGCCACAGGTGTCCGGAGCTGGCCAGAGCTATATACTGCGCTACGGAGGCGGCGGAGCGGTTGGAGCAGCCGGCAGCAGTGGCAGTTTAGCTAGCGTGACAGCCAGTAATCCCGCCACTGGAGGCGGCGGCTATCAGCCGGCATTGCGCGGCGGAGTGGCCGTATTTCCGCCCAATCCTATGGGAAATCAGGGCGGTGGCAATCTGCAGACGCAGCCCTCGCCACAGATCAAGCGGAAACAGACGCCAACGCGACCCATGAGCTTTGTGCGAGCGCTGGAGATGGCCGACTCCATGGAGATGCAGTCACTGGAACAGCAGCAAAACGGTGGCATTCCCGGCAGCGGGTCAGTGGGAAACAATCAACAGGCGGGCGGCGGTGGCGGTGGTCACCTAATGCAGAGCAACGCCTCCAATTCGGGCACTCCGGATAGAGCCAGCATCTACGACATGAACTACGAGATCTCCGTATAAcccgtggtggtgcctgtgctcctgatgccacccgccagccaagctccggggacagcagtcgctgctccaaccgctgccgttgctccagctgccatcgccggcccacagccgcaggggcagttttacaatgcccaactggagccggctgcctatgcctgtgttcctgtcttgcccctcaaaccgataagcaatggagtgggaaccaatagtcatcagcaccatcatcatcatcaccaacagcagcagcatcatcatcaccaacgtccgcaccagaacatccacaatcacaaccagattcagaaccagaaccagaaccagaaccagaacaaacgcaaattttcaaccttcttttagggaactaagggaactagccctagttcgtgaccttttttttacgaaattatttggagtggagagcatgtaagggtggcggccgcggctcgagggtacccggggatcctctagagtcgacctgcaggcatgcaagctattcgatgcacactcacattcttctcctaatacgataataaaactttccatgaaaaatatggaaaaatatatgaaaattgagaaatccaaaaaactgataaacgctctacttaattaaaatagataaatgggagcggcaggaatggcggagcatggccaagttcctccgccaatcagtcgtaaaacagaagtcgtggaaagcggatagaaagaatgttcgatttgacgggcaagcatgtctgctatgtggcggattgcggaggaattgcactggagaccagcaaggttctcatgaccaagaatatagcggtgagtgagcgggaagctcggtttctgtccagatcgaactcaaaactagtccagccagtcgctgtcgaaactaattaagtaaatgagtttttcatgttagtttcgcgctgagcaacaattaagtttatgtttcagttcggcttagatttcgctgaaggacttgccactttcaatcaatactttagaacaaaatcaaaactcattctaatagcttggtgttcatctttttttttaatgataagcattttgtcgtttatactttttatatttcgatattaaaccacctatgaagttcattttaatcgccagataagcaatatattgtgtaaatatttgtattctttatcaggaaattcagggagacggggaagttactatctactaaaagccaaacaatttcttacagttttactctctctactctagagtagctt
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